Browsing by Author "Cemek, M"

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Anti-Oxidant Activity in Measles
(Maney Publishing, 2005) Cemek, M; Bayiroglu, F; Çaksen, H; Cemek, F; Dede, S
Background: Measles leads to serious complications, especially in developing countries. Aim: Our aim was to evaluate the effect of oxidative stress on the enzymatic and non-enzymatic anti-oxidant status in children with measles. Methods: Levels of superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, albumin, total bilirubin and uric acid were studied in 17 children with measles and 29 healthy controls. Results and conclusions: There was a statistically significant difference between the groups for all parameters. All of the anti-oxidant activities except for albumin level were increased in the study group. Both enzymatic and nonenzymatic anti-oxidant defence systems were affected in measles.
Effects of Nigella Sativa and Vitamin E+se in Ccl4 Treated Rats
(indian veterinary Journal, 2004) Deger, Y; Sahin, A; Dede, S; Kilicalp, D; Cemek, M
Carbontetrachloride (CCl4), a selective hepatotoxic chemical agent has been utilized to produce liver cirrhosis in experimental animals (Parola et al., 1992). Vitamin E and Se are well-known liver preservative and hepatotrophic compounds (Zhang et al., 1996). Nigella sativa (black seed) is a seed of capsulated plants and belongs to the Ranunculaceae spp. Recently researches have shown the therapeutic effect of N. Sativa extracts such as bronchodilator, immunomodulative, antibacterial, hypotensive, choleretic, antitumoral, antifungal and anthelmentic (Salomi et al., 1992). The trace elements such as copper and zinc are co-factors of some enzymes, such as SOD which participate in antioxidant process by inhibiting reactive oxygen production (Bray and Bettger, 1990). The aim of this study was to determine the effect of N. sativa, well known therapeutic agent, vitamin E+Se administration on serum antioxidant vitamins (vitamin E, vitamin C, vitamin A, beta-carotene and retinol) and trace elements (Zn, Cu) concentrations on CCl4 treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats
(Wiley, 2003) Kanter, M; Meral, I; Dede, S; Cemek, M; Ozbek, H; Uygan, I; Gunduz, H
This study was designed to investigate the effects Nigella sativa L. (NS) and Urtica dioica L. (UD) on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in carbon tetrachloride (CCl4)-treated rats. A total of 60 healthy male Sprague-Dawley rats were utilized in this study. The rats were randomly allotted into one of four experimental groups: A (CCl4 -only treated), B (CCl4 + UD treated), C (CCl4 + NS treated) and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 [0.8 ml/kg of body weight, subcutaneously, twice a week for 90 days starting day 1]. In addition, B, C and D groups also received daily intraperitoneal injections of 0.2 ml/kg NS or/and 2 ml/kg UD oils for 45 days starting day 46. Group A, on the other hand, received only 2 ml/kg normal saline solution for 45 days starting day 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at beginning, at 45th and at 90th day of the experiment. The CCl4 treatment for 45 days increased the lipid peroxidation and liver enzymes, and also decreased the antioxidant enzyme levels. NS or UD treatments (alone or combination) for 45 days starting day 46 decreased the elevated lipid peroxidation and liver enzyme levels and also increased the reduced antioxidant enzyme levels. Live weights of the rats decreased in group A, and increased in groups B, C and D. It is concluded that NS and UD decrease the lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in the CCl4-treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats (Vol 50, Pg 264, 2003)
(Blackwell verlag Gmbh, 2003) Kanter, M; Meral, I; Dede, S; Gunduz, H; Cemek, M; Ozbek, H; Uygan, I
Evaluation of Antioxidant Status in Children With Acute Bacterial Meningitis and Encephalitis
(Taylor & Francis Ltd, 2003) Çaksen, H; Dede, S; Cemek, M; Dulger, H; Cemek, F
Antioxidant status was investigated in children with acute bacterial meningitis and encephalitis to investigate the possible role of free radicals in children with meningitis and encephalitis. Our study included 16 children with acute bacterial meningitis, 13 with encephalitis, and 17 control subjects. Serum ceruloplasmin, uric acid, albumin, bilirubin superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) levels were studied in all subjects within 6 h of admission. There was a statistically significant difference between the groups for all parameters except for serum uric acid. All antioxidant activities except for albumin level were increased in the study groups. Albumin level was higher in the control group than those of meningitis and encephalitis groups. When the values of meningitis and encephalitis were compared, there was a statistically significant difference between the groups for serum SOD, GPx, ceruloplasmin, and albumin. In conclusion, our study showed that serum SOD, GPx, catalase, and ceruloplasmin were higher in children with acute bacterial meningitis and serum SOD, GPx, catalase, ceruloplasmin, and total bilirubin levels were increased in children with encephalitis. These findings suggest that antioxidant status was almost similar in both acute bacterial meningitis and encephalitis conditions in childhood.
Investigation of Antioxidant Status in Children With Acute Otitis Media and Tonsillitis
(Elsevier Ireland Ltd, 2004) Cemek, M; Çaksen, H; Cemek, F; Bayiroglu, F; Dede, S; Dülger, H; Ustün, R
Objective: Antioxidant enzymes status was investigated in children with acute otitis media (AOM) and acute tonsillitis. The aim was to determine the effect of oxidative damage due to free radicals on the antioxidant enzymes status in children with AOM and acute tonsillitis. Methods: We measured erythrocyte levels of superoxide dismutase and glutathione peroxidase (GPx) and activity of serum catalase, ceruloplasmin, albumin and total bilirubin in 23 children with AOM, 27 with acute tonsillitis and 17 control subjects. Results: There was statistically significant difference between the groups for all parameters. ALL antioxidant activities except for albumin Level were increased in the studies groups. When the values of AOM and acute tonsillitis were compared, there was statistically significant difference between the groups for erythrocyte GPx. Conclusion: Our findings indicate that not only a few antioxidant enzymes but almost all antioxidant activities showed a significant enhancement in children with AOM and acute tonsillitis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
Investigation of the Lipid Peroxidation, Antioxidant Substances, Antioxidant Vitamins and Some Hematologic-Biochemical Parameter Levels in Gentamycine Induced Nephrotoxicosis in Dogs
(Tubitak Scientific & Technological Research Council Turkey, 2003) Ertekin, A; Karaca, M; Akkan, HA; Cemek, M; Ormanci, N
In our study, malondialdehyde, antioxidant vitamins, antioxidant substances and some hematologic-biochemical parameter levels in nephrotoxicosis were investigated. Six dogs were used in the study. Blood samples taken before the experiment were used as the control. Blood samples were taken on days 2, 5, 7 and 10 during the experiment. During analysis, we found that the statistical changes were not significant in the retinol and Vitamin C levels of the experiment group when compared to those of the control group. beta-carotene (P < 0.05) and Vitamin E (P < 0.001) levels were significantly decreased. Increases in the levels of malondialdehyde, seruloplasmin and urine gamma-glutamyl transferase (GGT) enzyme were P < 0.001, while decreases in the amounts of glutathione were P < 0.001. We also found that changes in erythrocyte and hemotocrit levels were not significant. On the other hand, increases in the levels of leucocyte (P < 0.01), creatinin and blood urea nitrogen (BUN) P < 0.001 were significant. In conclusion, oxidative damage can occur in cells in nephrotoxicosis, the use of antioxidants could be of benefit during treatment with chemotherapeutics.
An Investigation on Β-Carotene and Vitamin C Levels in Natural and Fruit Yogurts
(indian veterinary Journal, 2004) Agaoglu, S; Dede, S; Deger, Y; Cemek, M
Lipid Peroxidation and Antioxidant Levels, and Alpha Naphthyl Acetate Esterase Activity of Peripheral Blood Lymphocytes in Mallard, Muscovy and Pekin Ducks
(veterinarni A Farmaceuticka Univerzita Brno, 2006) Karaca, T; Cemek, M; Kanter, M
The aim of this study was to determine blood levels of malondialdehyde (MDA), reduced glutathione (GSH), ceruloplasmin and vitamin C, and the percentages of peripheral blood T-lymphocytes using the alpha-naphthyl acetate esterase (ANAE) method on Mallard (Anas platyrhynchos), Muscovy (Cairina moschata) and Pekin (Anas domestica) ducks. Blood samples were obtained from 8 adult ducks of each breed. The serum levels observed in Mallard, Muscovy and Pekin ducks respectively were 0.8, 1.07 and 1.3 nmol MDA per ml; 77.4, 66.9 and 78.7 mg GSH per 100 ml; 23.9, 26.1 and 24.1 mg ceruloplasmin per 100 ml; and 0.50, 0.52 and 0.70 mg vitamin C per 100 ml. The percentage of the ANAE (+) lymphocytes was 57.9%, 54.8% and 55.1% in Mallard, Muscovy and Pekin ducks, respectively. In this study, blood levels of lipid peroxidation and nonenzymatic antioxidants in the Mallard, Muscovy and Pekin ducks were determined.
Lipid Peroxidation and Antioxidant Status in Children With Acute Purulent Meningitis and Encephalitis
(Taylor & Francis Ltd, 2004) Çaksen, H; Cemek, M; Dede, S; Dulger, H; Cemek, F
In this study, lipid peroxidation and antioxidant status were investigated in children with acute bacterial meningitis and encephalitis. The aim was to determine whether there was a possible role of free radicals in meningitis and encephalitis in childhood. Our study included 16 children with acute bacterial meningitis, 13 with encephalitis, and 17 control subjects. Serum malondialdehyde (MDA), reduced glutathione (GSH), vitamin C, vitamin E, beta-carotene, and retinol levels were studied in all subjects within 6 h of admission. There was a statistically significant difference for serum MDA, GSH, and vitamin C between the groups. Serum MDA and vitamin C levels were higher, and serum GSH levels were lower in the study groups compared to the control group. Vitamin C levels were similar in both the encephalitis and control groups, but they were significantly lower in the children with encephalitis than the meningitis group. In conclusion, our study showed that serum MDA and GSH levels were affected in children with both meningitis and encephalitis, but vitamin C level was affected only in children with meningitis. Serum vitamin E, beta-carotene, and retinol levels were not changed in childhood meningitis and encephalitis.
Liquid Products From Verbascum Stalk by Supercritical Fluid Extraction
(Pergamon-elsevier Science Ltd, 2001) Cemek, M; Küçük, MM
Verbascum stalk mill was converted to liquid products by using organic solvents, such as methanol, ethanol and acetone, with catalysts (10% NaOH or ZnCl2) and without catalyst in an autoclave at temperatures of 533, 553 and 573 K. The liquid products were extracted by liquid-liquid extraction using benzene and diethyl ether. The yields from supercritical methanol, ethanol and acetone extractions were 44.4, 43.3 and 60.5 wt%, respectively, at 573 K. In the catalytic runs with methanol and ethanol, the extracts were 52.4 and 44.8% using 10% NaOH and 55.5 and 60.6% using ZnCl2, respectively, at 573 K. The yields from supercritical methanol increased from 38.2 to 52.4% as the temperature was increased from 533 to 573 K in the catalytic run. (C) 2000 Elsevier Science Ltd. All rights reserved.
Oxidant and Antioxidant Levels in Children With Acute Otitis Media and Tonsillitis
(Elsevier Ireland Ltd, 2005) Cemek, M; Dede, S; Bayiroglu, F; Çaksen, H; Cemek, F; Yuca, K
Objective: Recurrent episodes of acute otitis media (AOM) and acute tonsillitis (AT) are a common problem in infectious disorders during childhood and are major cause of morbidity in children. The organism maintains defense systems including nonenzymatic antioxidants such as Vitamins A, E and C and reduced glutathione (GSH) against reactive oxygen species (ROS). In the present study, Lipid peroxidation status and nonenzymatic antioxidant capacity were investigated in children with AOM and AT. Our aim was to compare the lipid peroxidation and responses of the body's antioxidant status in the closely associated infections such as AOM and acute tonsillitis. Methods: The study included 23 (14 mates, 9 females) children with AOM, 27 (14 mates, 13 females) with AT and 29 (16 mates, 13 females) healthy control subjects. The ages of the study and control subjects were between 2 and 7 years. Serum beta-carotene, retinol, Vitamin E, Vitamin C, and whole blood malondialdehyde (MDA) (as an indicator of lipid peroxidation) and GSH Levels were studied in all subjects. Results: There was a statistically significant difference between the groups for all parameters (P < 0.05). All of the antioxidant vitamins such as beta-carotene, retinol, Vitamin E, and Vitamin C levels were observed to be significantly decreased in the both patient groups. Nevertheless, GSH levels were also decreased in the patient groups. MDA levels were found to be higher in children with AOM and AT than in the healthy control subjects. When compared the AOM and AT groups, there was statistically significant difference between the groups for whole blood MDA (P < 0.05). Conclusion: Even though they seem to react in a similar way against ROS, in the cases of AOM and AT, it is possible to see the different approaches in these tissues for the oxidative stress. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Oxidation Products of Nitric Oxide and the Concentrations of Antioxidant Vitamins in Parasitized Goats
(Vysoka Skola veterinarni Farmaceuticka, 2002) Dede, S; Deger, Y; Kahraman, T; Deger, S; Alkan, M; Cemek, M
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E And C, beta-carotene and retinol) were determined spectrophotometrically in the blood serum of all Pats. The results were expressed as nitrate (mug/ml) 7.25 +/- 1.31-4.69 +/- 0.32; nitrite (mug/ml) 1.52 +/- 0.39-1.64 +/- 0.19; vitamin E (mg/100) 0.13 +/- 0.05-0.42 +/- 0.02; vitamin C (mg/100 ml) 1.49 +/- 0.26-1.46 +/- 0.15; retinol (g/100 ml) 201.51 +/- 15.69-234.081 +/- 45.15; beta-carotene (g/100 ml) 62.71 +/- 7.14-53.95 +/- 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.