Browsing by Author "Cemek, Mustafa"

Now showing 1 - 11 of 11

Altered Antioxidant Status and Increased Lipid Peroxidation in Children With Acute Gastroenteritis Admitted To a Pediatric Emergency Service
(Elsevier Science inc, 2009) Bayiroglu, Fahri; Cemek, Mustafa; Caksen, Hueseyin; Cemek, Fatma; Dede, Semiha
Acute gastroenteritis is a common illness worldwide and has a great impact on children. Our aim was to examine possible alterations in the antioxidant defense in pediatric gastroenteritis. To comprehensively examine the reaction of the antioxidant system, all possible components of the system were measured. The whole blood malondialdehyde and reduced glutathione, serum beta-carotene, retinol, vitamin C, vitamin E, catalase, ceruloplasmin, albumin, total bilirubin, uric acid, erythrocyte superoxide dismutase, and glutathione peroxidase levels were studied. Superoxide dismutase and glutathione peroxidase antioxidant enzyme activities and malondialdehyde levels were found to be increased, however, beta-carotene, retinol, vitamin C, vitamin E, reduced glutathione, and albumin levels were observed to be significantly decreased. Catalase activity remained unchanged, whereas some of the other non-enzymatic antioxidants such as ceruloplasmin, total billirubin, and uric acid levels were increased compared to the control group. We have shown an association between antioxidant levels and gastroenteritis in children. Further study is needed to assess whether antioxidant supplementation will be beneficial as an adjunct to conventional relevant therapy of the disease. (C) 2009 Elsevier Inc.
Bio-Element Status in Children With Acute Rheumatic Fever: Before Treatment and After Clinical Improvement
(Springer, 2010) Cemek, Mustafa; Buyukokuroglu, Mehmet Emin; Buyukben, Ahmet; Aymelek, Fatih; Yilmaz, Fatma; Dogan, Murat; Yildirim, Derya
Acute rheumatic fever (ARF) is an autoimmune multisystem disease. Bio-elements are required in different quantities by an organism to maintain its physiologic function. Monitoring the status of bio-elements is critical in human health. This study aimed to determine possible changes in levels of bio-elements in children with ARF before and after treatment. Levels of trace and major elements in children with ARF were investigated. The study included 33 children with ARF (17 boys and 16 girls) and 20 healthy control children (11 boys and 9 girls). The ages ranged from 5 to 16 years (mean 11.4 +/- A 3.82 years) in the study group and from 6 to 15 years (mean, 10.7 +/- A 3.22 years) in the control group. Trace and major element concentrations (total of 14 elements) in the serum were measured by inductively coupled plasma-optical emission spectroscopy. Before treatment, the levels of the major elements potassium (K) and magnesium (Mg) in children with ARF were higher than in the control group, whereas the calcium (Ca) level was lower. Before treatment, the levels of trace elements iron (Fe), selenium (Se), zinc (Zn), aluminum (Al), and barium (Ba) were lower, whereas the copper (Cu), beryllium (Be), cadmium (Cd), chromium (Cr), gallium (Ga), and strontium (Sr) levels were higher in the serum of the patients with ARF than in the control patients. The major findings show that the homeostasis of some trace and major elements were altered in the children with ARF and that these alterations may be a contributing factor in the pathogenesis of this disease.
Effect of Grape Seed Extract on Lipid Peroxidation, Antioxidant Activity and Peripheral Blood Lymphocytes in Rats Exposed To X-Radiation
(Wiley, 2007) Enginar, Hueseyin; Cemek, Mustafa; Karaca, Turan; Unak, Perihan
The present studies were designed to evaluate supplemental grape seed extract (GSE) and vitamin E supplements on lipid peroxidation, on antioxidant systems and peripheral blood lymphocytes in rats exposed to x-rays. Three groups of rats were investigated: a control group (CG) received intraperitoneal (i.p.)physiological serum I mL/day (n = 10), i.p.; a vitamin E group (VG) received 50 mg/kg/day (n = 10); an i.p. grape seed extract group received 50 mg/kg/day (n = 10). Four weeks later, a 6 Gy radiation dose was given to the rats. Blood samples were taken 24h later after irradiation and lymphocyte, malondialdehyde (MDA), reduced glutathione (GSH), nitrate, nitrite, reduced ascorbic acid, retinol, beta-carotene and ceruloplasmin concentrations were analysed. The levels of GSH (p < 0.05), retinol (p < 0.001), fi-carotene (p < 0.05) and ceruloplasmin concentration (p < 0.001) in the GSE group were found to be higher than in the control group but the level of MDA (p < 0.001) and nitrite concentration (p < 0.05) in rats supplemented with GSE were found to be lower than in the control group. The results indicate that GSE enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in blood samples of rats exposed to x-radiation. The antioxidant effect of GSE given to animals was more effective than vitamin E administered before whole-body irradiation in rats. Copyright (c) 2007 John Wiley & Sons, Ltd.
In Vivo Radioprotective Effects of Nigella Sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats
(Wiley-blackwell, 2006) Cemek, Mustafa; Enginar, Huseyin; Karaca, Turan; Unak, Perihan
Radiotherapy is one of the most common therapies for treating human cancers. Several studies have indicated that irradiation induces reactive oxygen species (ROS), which play an important role in radiation damage of the cell. It has been shown that Nigella sativa L. (NS) and reduced glutathione (GSH) have both an antiperoxidative effect on different tissues and a scavenger effect on ROS. The purpose of this study was to determine the antioxidant and radioprotective roles of INS and GSH against irradiation-induced oxidative injury in an experimental model. The INS group was administrated INS (1 mL/kg body weight), the GSH group was injected GSH (150 mg/kg body weight) and the control group was given physiologic saline solution (1 mL/kg body weight) for 30 consecutive days before exposure to a single dose of 6 Gy of radiation. Animals were sacrificed after irradiation. Malondialdehyde, nitrate, nitrite (oxidative stress markers) and ascorbic acid, retinol, beta-carotene, GSH and ceruloplasmin (nonenzymatic antioxidant markers) levels and peripheral blood lymphocytes were measured in all groups. There were statistically significant differences between the groups for all parameters (P < 0.05). Whole-body irradiation caused a significant increase in blood malondial-dehyde, nitrate and nitrite levels. The blood oxidative stress marker levels in irradiated rats that were pretreated with NS and GSH were significantly decreased; however, nonenzymatic antioxidant levels were significantly increased. Also, our results suggest that NS and GSH administration prior to irradiation prevent the number of alpha-naphthyl acetate esterase peripheral blood T lymphocytes from declining. These results clearly show that NS and GSH treatment significantly antagonize the effects of radiation. Therefore, NS and GSH may be a beneficial agent in protection against ionizing radiation-related tissue injury.
Investigation of Oxidant and Antioxidant Pathway Changes in Acute Rheumatic Fever
(Taylor & Francis Ltd, 2010) Uner, Abdurrahman; Sal, Ertan; Dogan, Murat; Sanli, Fatih M.; Acikgoz, Mehmet; Cemek, Mustafa; Cagan, Eren
Aim - The purpose of this study was to investigate the role of oxidant stress in the clinical process and pathogenesis of acute rheumatic fever (ARF). Material and methods - The study included 33 children with ARF and 20 healthy control subjects. The diagnosis of ARF was established according to the Jones Criteria. Malondialdehyde (MDA), reduced glutathione (GSH), alpha-tocopherol, ascorbic acid, retinol and beta-carotene levels were measured as markers of oxidative stress together with some antioxidant markers. Results - Our study includes 33 (19 male, 14 female) children with ARF and 20 (11 male, 9 female) healthy control subjects. The mean age ranged between 5-16 years and 5-15 years in the study and control groups, respectively. MDA was measured as 2.1 +/- 0.9 nmol/mL in the control group, 3.3 +/- 2.7 nmol/mL in the study group before treatment, and 2.1 +/- 1.2 nmol/mL after treatment. Blood GSH levels were 48.2 +/- 12.7 mg/dL in the control group, 24.7 +/- 16 mg/dL in the study group before treatment, and 40.6 +/- 21.3 mg/dL in the study group after treatment. MDA and GSH levels prior to the treatment were found to be significantly high and low as compared with the levels of the control group, respectively (P < 0.05, P < 0.001). After treatment, statistically important decrements and increments were determined in the levels of MDA (P < 0.05) and GSH (P < 0.01), respectively. Furthermore, alpha-tocopherol, retinol and beta-carotene levels prior to treatment in the study group, were significantly lower in comparison with control group levels (P = 0.05, P < 0.05, P < 0.01, respectively). Conclusion - We suggested that tissue damage in ARF may not only occur in the presence of increased oxidative stress, but also as a consequence of decreased antioxidant markers.
Köpeklerde Gentamisin Nefrotoksikozisinde Lipit Peroksidasyonu Antioksidan Maddeler, Antioksidan Vitaminler ve Bazı Hematolojik- Biyokimyasal Parametre Düzeylerinin Araştırılması
(2003) Ormancı, Neslihan; Cemek, Mustafa; Akkan, Hasan Altan; Ertekin, Ali; Karaca, Mehmet
Bu çalışmada, nefrotoksikoziste malondialdehit (MDA), antioksidan vitaminler, antioksidan maddeler ve bazı hematolojik-biyokimyasal parametre seviyeleri araştırıldı. Çalışmayı altı sokak köpeği oluşturdu. Uygulama öncesi alınan kanlar kontrol grubunu oluşturmak amacıyla kullanıldı. Deneme süresince 2., 5., 7. ve 10. günlerde kan örnekleri alındı. Yapılan analizlerde kontrol grubu değerlerine göre deneme grubu retinol ve Vit C düzeylerindeki değişimlerin istatistik olarak anlamlı olmadığı, ß-karotin (P<0,05) ve Vit E' deki (P<0,001) düşüşlerin önemli olduğu tespit edildi. Malondialdehit, serüloplazmin ve idrar $\\gamma$- glutamil transferaz (GGT) enzim seviyelerinde gözlenen artışların P<0,001; glutatyon miktarlarında saptanan düşmelerin P<0,001 düzeylerinde anlam ifade ettikleri saptandı. Eritrosit ve hematokrit değerlerinde gözlenen değişimlerin önemli olmadığı, lökosit miktarlarındaki artışın P<0,01 ; kreatinin ve kan üre nitrojeni (BUN)' ndeki artışın ise P<0,001 düzeyinde önemli olduğu da gözlendi. Sonuç olarak, nefrotoksikoziste hücrelerde oksidatif hasarın şekillenebileceği ve bu nedenle hastalığın tedavisi süresince kullanılan ilaçlarla beraber antioksidanların da kullanılmasının yararlı olacağı kanaatine varıldı.
Oxidant and Antioxidant Status in Children With Subacute Sclerosing Panencephalitis
(Sage Publications inc, 2014) Caksen, Huseyin; Ozkan, Mustafa; Cemek, Mustafa; Cemek, Fatma
We analyzed serum alpha-tocopherol, beta-carotene, retinol, and ascorbic acid levels and malondialdehyde and reduced glutathione concentrations on erythrocyte and cerebrospinal fluid in 30 patients with subacute sclerosing panencephalitis to evaluate oxidant and antioxidant status. Serum alpha-tocopherol, beta-carotene, retinol, ascorbic acid levels, and erythrocyte and cerebrospinal fluid reduced glutathione concentrations were decreased; however, erythrocyte and cerebrospinal fluid malondialdehyde levels were increased in the patients. Cerebrospinal fluid malondialdehyde levels were different between clinical stages of the disease (P < .05). Higher cerebrospinal fluid malondialdehyde level was associated with the more severe clinical stage. A positive correlation was found between cerebrospinal fluid malondialdehyde level and clinical stages (r = 0.42; P < .05) and between erythrocyte malondialdehyde level and clinical stages (r = 0.40; P < .05). Our findings showed presence of oxidative damage in subacute sclerosing panencephalitis and that antioxidants were increased as defense mechanisms of the organism against oxidative damage.
Oxidant and Non-Enzymatic Antioxidant Status in Measles
(Oxford Univ Press, 2007) Cemek, Mustafa; Dede, Semiha; Bayiroglu, Fahri; Caksen, Hueseyin; Cemek, Fatma; Mert, Nihat
Measles is a highly contagious viral disease of childhood associated with serious complications and significant morbidity and mortality. In the present study, antioxidant and oxidant status was investigated in children with measles. Our aim was to evaluate the possible changes in non-enzymatic antioxidant levels and their relationship to the oxidative stress in measles. According to our knowledge, there are no data about the blood levels of malondialdehyde (MDA), reduced glutathione (GSH) and vitamin C in measles. We measured the whole blood MDA (as a marker of lipid peroxidation) and GSH, and serum beta-carotene, retinol, alpha-tocopherol and ascorbic acid levels in all subjects. There was a statistically significant difference between the groups for all parameters (p < 0.05). Non-enzymatic antioxidant status was found to be decreased but, lipid peroxidation was increased in the study group. As a conclusion, these findings suggested that oxidant and antioxidant defence system were altered in children with measles.
Oxidative Stress and Enzymic-Non Antioxidant Responses in Children With Acute Pneumonia
(Wiley, 2006) Cemek, Mustafa; Caksen, Huseyin; Bayiroglu, Fahri; Cemek, Fatma; Dede, Semiha
In this article, oxidative stress and enzymic-non-enzymic antioxidants status were investigated in children with acute pneumonia. Our study included 28 children with acute pneumonia and 29 control subjects. The age ranged from 2 to I I years (4.57 +/- 2.13 years) and 2 to 12 years (4.89 +/- 2.22 years) in the study and control groups, respectively. Whole blood malondialdehyde (MDA) and reduced glutathione (GSH), serum beta-carotene, retinol, vitamin C, vitamin E, catalase (CAT), ceruloplasmin (CLP), total bilirubin, erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels were studied in all subjects. There was a statistically significant difference between the groups for all parameters except for serum CAT. Whole blood MDA, serum CLP and total bilirubin levels were higher in the study group than those of the control group. However, SOD, GPx, beta-carotene, retinol, vitamin C, vitamin E and GSH levels were lower in the study group compared with the control group. All antioxidant vitamin activities were decreased in children with acute pneumonia. Our study demonstrated that oxidative stress was increased whereas enzymic and non-enzymic antioxidant activities were significantly decreased in children with acute pneumonia. Copyright (c) 2005 John Wiley & Sons, Ltd.
Protective Potential of Royal Jelly Against Carbon Tetrachloride Induced-Toxicity and Changes in the Serum Sialic Acid Levels
(Pergamon-elsevier Science Ltd, 2010) Cemek, Mustafa; Aymelek, Fatih; Buyukokuroglu, Mehmet Emin; Karaca, Turan; Buyukben, Ahmet; Yilmaz, Fatma
Royal Jelly (RJ) is used in the Turkish folk medicine for the treatment of number of disorders. The present study describes the hepatoprotective and antioxidant activities of the RJ against carbon tetrachloride (CCl4)-induced acute liver damage. Sprague-Dawley rats were used for the experiment. CCl4 (0.8 ml/kg; s.c.) and RJ (50, 100, 200 mg/kg: orally) were given every other day, for 20 days. Malondialdehyde, reduced glutathione in whole blood and tissues: ceruloplasmin, sialic acid, ascorbic acid, retinol, beta-carotene and liver enzymes levels in serum were measured. Additionally, histopathological alterations in the liver were examined. RJ exerted the significant protective effect on liver damage as well as on oxidative stress induced by CCl4, resulting in reduced lipid peroxidation and improved endogenous antioxidant defence systems. It also reduced the elevated levels of liver enzymes. Histopathological study further confirmed the hepatoprotective effect of RJ, when compared with the CCl4 treated control groups. In conclusion, present study reveals biological evidence that supports the use of RJ in the treatment of chemical-induced hepatotoxicity. (C) 2010 Elsevier Ltd. All rights reserved.
Relationship Between Antioxidant Capacity and Oxidative Stress in Children With Acute Hepatitis a
(Baishideng Publishing Group inc, 2006) Cemek, Mustafa; Dede, Semiha; Bayiroglu, Fahri; Caksen, Hueseyin; Cemek, Fatma; Mert, Nihat
AIM: To investigate in children with acute hepatitis A. According to our knowledge, there are no data about the blood levels of malondialdehyde (MDA, an indicator of oxidative stress) and nonenzymic antioxidants in children with acute hepatitis A. METHODS: Whole blood MDA and reduced glutathione (GSH), serum beta-carotene, retinol, vitamin E and vitamin C levels were studied in 19 (10 females, 9 males) children with acute hepatitis A and in 29 (13 females, 16 males) healthy control subjects. RESULTS: There was a statistically significant difference between patients and controls for all parameters (P < 0.05). Lipid peroxidation marker MDA was significantly elevated (P < 0.001), while antioxidants beta-carotene, retinol and GSH were significantly decreased (all P < 0.001) in patients compared to healthy subjects. In addition, a-tocopherol and ascorbic acid levels were significantly lower in patients when compared to age and sex matched controls (P < 0.05, P < 0.01, respectively). CONCLUSION: Our study shows that hepatitis A virus induces oxidative stress in childern with hepatitis A. This finding could be taken into consideration to improve the therapeutic approach in acute hepatitis A. (C) 2006 The WJG Press. All rights reserved.