Browsing by Author "Cengiz, Zeynep Tas"

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Comparison of Microscopy, Elisa, and Real-Time Pcr for Detection of Giardia Intestinalis in Human Stool Specimens
(Tubitak Scientific & Technological Research Council Turkey, 2017) Beyhan, Yunus Emre; Cengiz, Zeynep Tas
Background/aim: This study included patients who had digestive system complaints between August 2015 and October 2015. The research was designed to compare conventional microscopy with an antigen detection ELISA kit and the TaqMan-based real-time PCR (RT-PCR) technique for detection of Giardia intestinalis in human stool specimens. Materials and methods: Samples were concentrated by formalin-ether sedimentation technique and microscopic examinations were carried out on wet mount slides. A commercially available ELISA kit (Giardia CELISA, Cellabs, Brookvale, Australia) was used for immunoassay. DNA was extracted from fecal samples of about 200 mg using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, Hilden, Germany) and the LightCycler Nano system (Roche Diagnostics, Mannheim, Germany) was used for the TaqMan-based RT-PCR assay. Results: A total of 94 stool samples, 38 of them diagnosed positive (40.4%) and 56 of them diagnosed negative by microscopy, were selected for evaluation by antigen detection and molecular assays. The prevalence of G. intestinalis infection was found as 46.8% (n: 44) and 79.8% (n: 75) by ELISA and RT-PCR, respectively. RT-PCR revealed by far the highest positivity rate compared to the other two methods. The difference between these methods was found to be statistically significant (P < 0.05). In comparison to PCR, the sensitivity and specificity of microscopy and ELISA were 50.7% and 100% and 53.3% and 79%, respectively. Conclusion: RT-PCR seems to be much more sensitive and beneficial for rapid and accurate diagnosis of G. intestinalis in human stools.
Demodex Species Positivity Among Patients With Cancer, on Hemodialysis and With Diabetes Mellitus
(Southeast Asian Ministers Educ Organization, 2017) Mutlu, Fatma Yola; Cengiz, Zeynep Tas
The objective of the present study was to determine the prevalence of Demodex sp among patients with cancer, on hemodialysis and diabetes mellitus and among controls in order to investigate if there are significance differences in Demodex sp positivity. The study was conducted at Ataturk University Research and Application Hospital, Turkey and the Yuzuncu Yil University Parasitology Laboratory, Turkey between 22 August 2011 and 31 May 2016. Study subjects consisted of 50 patients with cancer, 50 patients diagnosed with chronic renal failure and were on hemodialysis, 50 patients with diabetes mellitus and 75 healthy controls. Each patient and each control had a skin surface biopsy using a slide with cyanoacrylate applied wet to the skin until it dried and then was removed and examined under light microscopy at x10 and x40 magnification. A positive sample was one in which mites / 1 cm(2) of skin were identified. Twenty percent of the total patient group (n=150) and 5.3% of the control group (n=75) had a positive result. Among the patient groups, 26% of cancer patients, 22% of diabetes mellitus patients and 12% of patients on hemodialysis were positive. A significant association was seen between a positive skin biopsy for Demodex sp and cancer (p<0.01) and between a positive biopsy and diabetes mellitus (p<0.01). In conclusion, patients with cancer and diabetes mellitus are more likely to have a positive skin biopsy for Demodex sp than controls.
Evaluation of Some Chronical Diseases in Etiopathogenesis of Demodicosis
(Elsevier Taiwan, 2017) Cengiz, Zeynep Tas; Ozkol, Hatice Uce; Beyhan, Yunus Emre; Ozturk, Murat; Yilmaz, Hasan
Background: The aim of this study is to assess the effect of chronic diseases on the etiopathogenesis of demodicosis through the determination of Demodex sp. positivity in the face and eyelashes of the people with the chronic disease. Methods: This study is conducted on the patients who were referred to the Laboratory of Parasitology from the Yuzuncu Yil University, Faculty of Medicine, Outpatient Clinic of Dermatology. The samples were taken from the patients with standard skin surface biopsy. Furthermore, eyelashes of the patients were examined for Demodex sp. Result: When the patient groups are considered, demodicosis was found in the ratio of 18.5% of the patients with diabetes mellitus (p < 0.05), in 27% of those with hypertension (p < 0.01), in 25% of those with cancer (p < 0.01), in 32.5% of those with chronic renal failure (p < 0.01), in 23.7% of those with hypothyroidism (p < 0.05) and in 22.2% of the patients with hepatitis B (p < 0.05). Demodex sp. positivity was determined in the eyelashes of eight patients (p > 0.05). Conclusion: It is concluded that, in such patients the presence of this parasite should be taken into consideration in case of dermatoses occurring on the face, and these patients could be under the risk of occurring blepharitis as well. Copyright (C) 2017, Taiwanese Dermatological Association. Published by Elsevier Taiwan LLC.
Evaluation of the Relationship Between Histopathological Findings in Appendectomy Specimens and Enterobius Vermicularis Infection
(Erciyes Univ Sch Medicine, 2023) Aydemir, Selahattin; Gumus, Meryem; Inceman, Merve; Cengiz, Zeynep Tas; Bayir, Mehmet Hafit; Yilmaz, Hasan
Objective: This study aimed to assess the relationship between Enterobius vermicularis and appendicitis by determining the frequency of E. vermicularis in appendectomy specimens obtained after a preliminary diagnosis of appendicitis. Materials and Methods: Retrospective evaluation was conducted on the results of appendectomy specimens examined histopathologically at Van Training and Research Hospital Pathology Laboratory from March 2016 to March 2022. Hematoxylin-eosin stained slides from 7,814 appendectomy specimens were scrutinized for the presence of E. vermicularis, acute inflammation, fecaloid material, lymphoid hyperplasia, obliteration, perforation, phlegmonous appendicitis, and peritonitis. Furthermore, slides stained with hematoxylin-eosin (H&E) from patients testing positive for E. vermicularis were re-evaluated by the author in pathology.Results: E. vermicularis was identified in the appendectomy specimens of 50 (0.64%) out of 7,814 patients who underwent appendectomies. In histopathological examina-tions, the occurrence of lymphoid hyperplasia and fibrous obliteration was higher in E. vermicularis-positive patients compared to those who teste negative. Findings revealed that the mean white blood cell count (WBC) was lower in E. vermicularis-positive patients than in negative patients, while the mean eosinophil percentage (EO%) was higher.Conclusion: These findings imply that although the parasite is not a direct causative factor for appendicitis, it may contribute to the development of factors that result in obstruction in certain cases.
Evaluation of Toxoplasma Gondii in the Etiology of Cryptogenic Epilepsy: a Case-Control Study
(Galenos Publ House, 2024) Aydemir, Selahattin; Afshar, Milad Torkamanian; Afshar, Ozlem Sari Torkamanian; Cengiz, Zeynep Tas; Kiraz, Mustafa; Baydar, Caner; Yilmaz, Hasan
Objectives: This study was conducted to evaluate the relationship between toxoplasmosis and cryptogenic epilepsy (CE) by comparing the rate of Toxoplasma gondii seropositivity identified in CE patients with those without any neurological disorder. Methods: The study included 200 cryptogenic patients and 164 individuals without neurological disorders. T. gondii seropositivity was studied in individuals Results: The study found T. gondii IgG in 15.50% of CE patients and 28.66% of individuals without any neurological disorder. The difference between the two Conclusion: Toxoplasmosis did not increase the risk of epilepsy in the region where this study was conducted. However, for a better understanding of the CE-T. gondii relationship, we believe that seroprevalence studies should be conducted in larger populations.
The Frequency of Cryptosporidium Spp. in Immunocompromised Patients by Modified Acid-Fast Staining, Cassette Kit and Elisa Methods: Comparison of the Diagnostic Techniques
(Ahvaz Jundishapur Univ Med Sci, 2017) Cengiz, Zeynep Tas; Yilmaz, Hasan; Sahin, Ibrahim Halil; Kapmaz, Mahir; Ekici, Pinar
Background: Cryptosporidiosis has been reported in both immunocompetent and immunocompromised patients from over 40 countries in six continents. Objectives: This study was carried out to determine the prevalence of Cryptosporidium spp. in immunocompromised patients by methods of modified acid fast staining, ELISA and Cassette Kit, and to also compare the three methods. Methods: The patients in different age groups admitted to Bitlis state hospital between June 23, 2011 and January 13, 2015 were enrolled. The study group was composed of 300 immunocompromised volunteer patients and the control group of 100 volunteers with normal immune system. In the study group, patients with hemodialysis, chronic renal failure, diabetes mellitus, diabetes insipidus, and cancer were enrolled. Formalin-ethyl acetate, modified acid fast staining and native-lugol were used for all stool samples; they were also investigated for Cryptosporidium antigens by the ELISA and Cassette Kit. Results: The highest prevalence rate of Cryptosporidium spp. is found to be 11.3%, which is obtained by ELISA and the lowest (0.7%) by Cassette Kit in the study group. All patients with cryptosporidiosis had diarrhea. The highest prevalence rate (20%) was observed in patients with diabetes insipidus and the second highest in patients with chronic renal failure (11.5%) by ELISA. The sensitivities and specificities of Cassette Kit and modified acid fast staining were 5.9%, 100%; and 50%, 100%, respectively. Conclusions: It is concluded that Cryptosporidium spp. should be considered in immunocompromised diarrheal patients and ELISA must be chosen for detection of this parasite. Modified acid fast staining and Cassette Kit techniques could be performed if ELISA is not available. The single use of the Cassette Kit will be inadequate for the diagnosis of cryptosporidiosis.
Human Infection With Dicrocoelium Dendriticum in Turkey
(K Faisal Spec Hosp Res Centre, 2010) Cengiz, Zeynep Tas; Yilmaz, Hasan; Dulger, Ahmet Cumhur; Cicek, Mutalip
Human dicrocoeliosis is reported sporadically in various parts of the world. We report a case in a 21-year-old male, who had right upper abdominal pain, weight loss, and chronic relapsing watery diarrhea three to four times daily for four weeks. The patient had abdominal tenderness to palpation in the right upper quadrant. Alkaline phosphatase, alanine aminotransferase, and serum immunoglobulin E levels were slightly elevated; all other biochemical and hematological findings were in their normal ranges. The duodenal biopsy samples were normal and an abdominal ultrasonography showed no biliary or hepatic abnormality. Stool microscopy revealed numerous eggs of Dicrocoelium dendriticum. As pseudoparasitosis can result from eating raw, infected animal liver, the patient was given a liver-free diet for three days, to rule out that possibility. Subsequent stool examinations showed eggs in each of the samples indicating that the infection was genuine. The patient was treated with triclabendazole 10 mg/kg in a single dose. Four weeks later, no parasite eggs were detected in the microscopic examination of the stool samples. The patient got better gradually and the symptoms disappeared. Physicians should keep in mind parasitic diseases such as the rarely encountered dicrocoeliosis.
Investigation of Blastocystis Hominis Frequency in Patients With Diabetes by Microscopy and Conventional PCR Methods
(Iranian Scientific Society Medical Entomology, 2023) Sahin, Maksut; Cengiz, Zeynep Tas; Halidi, Ahmed Galip; Aydemir, Selahattin
Bu çalışmanın amacı, diyabetli hastalarda Blastocystis hominis sıklığını mikroskopi ve konvansiyonel PCR yöntemleri ile belirleyip bu yöntemleri karşılaştırmak ve bu hastalarda hangi subtipin daha yaygın olduğunu saptamaktır. Van Yüzüncü Yıl Üniversitesi Dursun Odabaş Tıp Merkezi'nin Dahiliye Polikliniklerinde muayene olan ya da servislerinde yatış yapan toplam 150 diyabetli hasta ve herhangi bir kronik hastalığı olmayan sağlıklı 100 kişinin dahil edildiği çalışma, 01.01.2020-30.11.2021 tarihleri arasında yürütüldü. Diyabetli hastaların 67 (%44,6)'si erkek, 83 (%55,3)'ü kadınlardan; kontrol grubunun 57 (%57)'si erkek, 43 (%43)'ü kadınlardan oluştu. Örnekler PCR ve mikroskopi (nativ-Lugol ve trikrom boyama) ile değerlendirildi. Çalışmamızda 150 diyabetli hastanın 38'inde (%25,3) PCR ile, 34'ünde (%22,7) nativ-Lugol ve trikrom boyama ile B. hominis saptandı. Kontrol grubunda 100 kişinin 14'ü (%14) PCR ile, 10'u (%10) nativ-Lugol ve trikrom boyama ile pozitif bulundu. Üç yöntem dikkate alındığında diyabetli hastaların 40'ında (%26,7), kontrol grubunun 14'ünde (%14) B. hominis pozitifliği saptandı (p= 0,011). Diyabetli hastalar ve kontrol grubundan oluşan toplam 250 olgunun %20,8'inde (52 olgu) PCR ile, %17,6'sında (44 olgu) nativ-Lugol ve trikrom boyama ile B. hominis saptandı. PCR altın standart kabul edildiğinde mikroskopinin sensitivitesi %80,76, spesifitesi %98,99; mikroskopi altın standart kabul edildiğinde PCR'ın sensitivitesi %95,45, spesifitesi %95,14 olarak hesaplandı. Yapılan istatistiksel değerlendirmede cinsiyet (p= 0,023), yaş (p= 0,045; ≤35 ve >35 karşılaştırması), diyabet süresi (p= 0,04) ve HbA1c değeri (p= 0,023; <8 ve ≥8 karşılaştırması) ile B. hominis pozitifliği arasında anlamlı fark bulundu. Diyabetli hastalara ait 13 örneğin 10'unda (%76,9) ST1, üçünde (%23,07) ST2 belirlendi. Sonuç olarak diyabetli hastalarda B. hominis'in dikkate alınması gereken bir etken olduğu; 10 yıldan daha uzun süre diyabetli olanların bu parazit için daha fazla risk altında olduğu; tanıda PCR'ın yüksek sensitivite ve spesifiteye sahip olduğu ve mümkünse nativ-Lugol ya da trikrom boyama ile beraber kullanılmasının uygun olacağı anlaşılmıştır. Çalışmamızda asemptomatik diyabetli hastalarda en sık saptanan alt tip ST1 olarak belirlenmiş olmakla beraber bu sonuç diyabetli hastalarda B. hominis'in patojenitesinde alt tip faktörünün önemini ortaya koymak için yeterli görülmemiştir. Bu kapsamda hem diyabetli hem de immünitesi bozulmuş diğer hasta gruplarında çok daha geniş kapsamlı çalışmalar yapılmasına ihtiyaç vardır. Anahtar Kelimeler: Blastocystis hominis, Diyabet, Mikroskopi, PCR, Alt tip
Investigation of the Presence of Enterocytozoon Bieneusi and Encephalitozoon Intestinalis in Immunosuppressed Patients With Diarrhea by Ifa and Real Time Pcr Methods
(Elsevier, 2023) Aydemir, Selahattin; Halidi, Ahmed Galip; Ekici, Abdurrahman; Cengiz, Zeynep Tas
Purpose: This study aimed to investigate the frequency of Enterocytozoon bieneusi and Encephalitozoon intestinalis in patients with diarrhea in the immunosuppressed.Methods: Patients between the ages of 18-85 who applied to different clinics of Mus, Bulanik and Bitlis State Hospitals and were referred to the microbiology or parasitology laboratory were selected for this study. A total of 200 individuals, including 88 immunosuppressed with diarrhea patients, 38 immunocompetent with diarrhea patients, 38 immunosuppressed without diarrhea patients, and 36 immunocompetent without diarrhea in-dividuals, were included. Collected stool samples were evaluated using IFA-MAbs and real-time PCR methods to determine the frequency of E.intestinalis and E.bieneusi.Results: E. intestinalis was detected in 59 (29.5%) of 200 samples and E. bieneusi was detected in 46 (23.0%) of them. Mixed infection was detected in 16 (8%) of the positive samples. While there was no statistically significant difference between E. intestinalis positivity and gender, age, diarrhea status and immune system status, a statis-tically significant relationship was determined between E. bieneusi positivity and diarrhea. When the real-time PCR method was accepted as the gold standard, the sensitivity of the IFA-MAbs method in the diagnosis of E.intestinalis was 94.54%, the specificity was 97.24, the sensitivity in the diagnosis of E. bieneusi was 95.45%, and the specificity was 98.72%. The overall accuracy of the IFA-MAbs method was 96.5% for the diagnosis of E.intestinalis and 98% for the diagnosis of E. bieneusi.Conclusions: The findings suggest that E. intestinalis and E. bieneusi should be considered in both immunosup-pressed and healthy individuals with diarrhea. IFA-MAbs method can be used in addition to the real-time PCR method to diagnose E. intestinalis and E. bieneusi.
Prevalence of Cystic Echinococcosis in Slaughtered Sheep and Goats in Ahar Abattoir, Northwest Part of Iran
(Kafkas Univ, veteriner Fakultesi dergisi, 2010) Lotfi, Alireza; Yusefkhani, Mansour; Samavatian, Ali; Yilmaz, Hasan; Cengiz, Zeynep Tas; Valilou, Mohammadreza
The aim of this study was to investigate the prevalence of cystic echinococcosis (CE) of slaughtered sheep and goats in Ahar Abattoir, Northwest of Iran. Out of 7.868 slaughtered animals examined for CE (hydatidosis) in liver and lungs (7.654 sheep and 214 goats), 25.40% were infected. The rates of lungs and liver CE were 15.28% and 6.08% in sheep, respectively, and 19.15% and 10.28% in goats, respectively. The infection rate was higher in lungs and was more pronounced in goats. Existence of infected stray dogs in grassland and ruminant grazing on infected pasture are two main reasons for high infection rates in Arasbaran region.
The Problem of Cystic Echinococcosis in Van Province
(Kafkas Univ, veteriner Fakultesi dergisi, 2009) Yilmaz, Hasan; Cengiz, Zeynep Tas; Cicek, Mutalip
This study was carried out prospectively in Van Meat Company and Van Municipality Slaughterhouse, Eastern Anatolian Region of Turkey. In the study, liver, lungs, spleen, hearth and other internal organs of 552 cattle and 1613 sheep slaughtered were inspected for cystic echinococcosis (CE). The organs were examined carefully with hands and the organs and cysts were cut with knife for confirmation of diagnose. It has been detected that 486 cattle and 1150 sheep slaughtered were entered to Van province from the Eastern border of Turkey. and 66 cattle and 463 sheep were breed in Van. Z test was used to evaluate the relationship of the CE's prevalence between the animals entered to Van province from the Eastern border of Turkey and breed in Van. CE was found in 22.63% of 486 cattle and 67.57% of 1150 sheep entered to Van province from Eastern border of the Country. The infection was determined in 6.06% of 66 cattle and in 28.94% of 463 sheep breed in Van. CE was totally detected in 20.65% of 552 cattle and in 56.48% of 1613 sheep. A significant independent relationship was found between the animals entered from the Eastern border of Turkey and breed in Van for CE's prevalence (P < 0.001).
Seroprevalence of Human Fascioliasis in Van Province, Turkey
(Aves, 2015) Cengiz, Zeynep Tas; Yilmaz, Hasan; Dulger, Ahmet Cumhur; Akdeniz, Hayrettin; Karahocagil, Mustafa Kasim; Cecek, Mutalip
Background/Aims: Fasciola hepatica is a rare zoonotic parasite that infects the liver of many mammals including humans. The aim of this study was to determine the seroprevalence of fascioliasis in Van province by ELISA (antibody detection) on the assumption that not all cases could be detected by stool examination alone. Materials and Methods: A total of randomly selected 1,600 patients, directed from affiliated outpatient clinics to Yuzuncu Yil University Medical Faculty Parasitology Laboratory, were enrolled in the study. Their mean age was 4.44 +/- 19.00 years. Blood samples were collected from all the patients, and their stool samples were examined. For the stool examination, native-lugol and sedimentation (in formalin-ethyl acetate) methods were employed. ELISA for F. hepatica was performed on the blood samples from all patients. Seropositive patients were treated with triclabendazole. Results: F. hepatica was detected by ELISA in 89 (5.6%) of the 1,600 patients, but eggs were identified on the stool examination in only 29 (1.8%) patients. The prevalence of F. hepatica was higher in females (7.2%) than in males (4.2%) and was higher in the >= 36-year age group (6.7%) than in the <= 35-year age group (4.4%). Abdominal pain (93.3%), fatigue (88.8%), and weight loss (69.7%) were the most common symptoms. Eosinophilia was present in 89.9% of the patients. All seropositive patients had a history of eating raw aquatic plants. Conclusion: Stool examination alone is not sufficient to diagnose F. hepatica. Serological tests such as ELISA must be used together with stool examination.
Seroprevalence of Toxocariasis in Cryptogenic Epilepsy Patients
(Parlar Scientific Publications (p S P), 2021) Ekici, Abdurrahman; Cengiz, Zeynep Tas; Cagac, Aydin; Yilmaz, Hasan; Beyhan, Yunus Emre; Yilgor, Abdullah
This study was conducted to determine the seroprevalence of Toxocara canis and Toxocara call by ELISA in cryptogenic epilepsy patients. The research was conducted between May 2015 and January 2018 at the Van Yfizfincti Yil University Medical Faculty.Departments of Parasitology and Neurology. The study included 100 patients diagnosed with cryptogenic epilepsy at the Neurology Policlinic of the DooyuuOdxhuy Medical Center, Van Yfiziincii Yil University. The cryptogenic epilepsy patient and control groups consisted of individuals who did not exhibit other helminths on their stool examinations and had not received any previous parasite therapy. Blood samples from the cryptogenic epilepsy patients and control group were investigated for of anti T cants positivity using ELISA. Anti -T. cants positivity was detected in 24% of patients with cryptogenic epilepsy and in 12% of the control group. Blood samples of the cryptogenic epilepsy patients and control group with toxocariasis were also examined for Fasciola hepatica. F. hepatica was not detected in any of the subjects with toxocariasis. In this study, there was a statistically significant relationship between cryptogenic epilepsy and toxocariasis in the comparison of the patient and control groups (p < 0.05). There was also a statistically significant relationship between toxocariasis and gender and eosinophilia (p < 0.05). However, although some complaints and symptoms, such as urticaria, abdominal pain, chronic fatigue, headache, and nervousness, were detected in some patients, no statistically significant relationship was found between these complaints and toxocariasis. It was concluded that toxocariasis should also he considered in patients with cryptogenic epilepsy, and individuals should avoid contact with dog and cat feces, in order to protect themselves from contracting this disease. In addifion, fruits and vegetables suspected of possible contamination with the feces of these animals should be washed with an abundance of clean water. Additional future studies are required in this field.
Seroprevalence of Toxocariasis in Cryptogenic Epilepsy Patients (Vol 30, Pg 6371, 2021)
(Parlar Scientific Publications (p S P), 2022) Ekici, Abdurrahman; Cengiz, Zeynep Tas; Cagac, Aydin; Yilmaz, Hasan; Beyhan, Yunus Emre; Yilgor, Abdullah
Single-Strand Conformation Polymorphism-Based Genetic Characterization of the Cyclospora Cayetanensis Strains Collected From Different Provinces in Turkey
(inst Agricultural Medicine, 2021) Cicek, Muttalip; Yildirim, Ibrahim Halil; Cengiz, Zeynep Tas; Karaman, Ulku
Introduction and objective. Cyclospora cayetanensis, a coccidian protozoan species, has been recently found to cause diarrhea in all age groups in immunocompetent and immunocompromised individuals in most regions of the world. This study aimed to conduct the molecular detection of C. cayetanensis and to determine the genetic diversity of the 18S ribosomal RNA (rRNA) gene sequence of C. cayetanensis isolated from individuals living in different provinces in Turkey by using PCR-single-strand conformation polymorphism (SSCP). Materials and method. A total of 22 subjects were included in the study. Fourteen of the subjects were female and eight were male, with ages ranging between 7-65 years. Stool specimens were examined using wet mount and modified acid-fast staining methods, which revealed the presence of oocysts in the samples. The 18S rRNA ITS-1 Ccits37f-GCTTGCTATGTTTTAGCATGTGG and Ccits501r-GCACAATGAATGCACACACA gene regions were used as primers. The PCR products were analyzed by agarose gel electrophoresis and visualized on a UV transilluminator. For the SSCP, the PCR products were denatured with formamide, run for 16 h in 6% (49:1) polyacrylamide gel, and then imaged with silver staining. Results. SSCP assay was performed given that the DNA strands demonstrated different folds; the DNA strands contain different nucleotides based on the PCR-SSCP results for the Cyclospora strains collected in 4 provinces. Moreover, 3 different band profiles were observed in the investigated samples. A slight mutation difference was observed among the strains collected. Conclusions. Further comprehensive studies involving more C. cayetanensis-positive specimens and utilizing different mutation screening methods are warranted to demonstrate mutation differences in Cyclopora strains in Turkey.