Browsing by Author "Cetin, Nebi"

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Anti-Mullerian Hormone Concentrations Can Be Reliably Determined by a Single Measurement, Irrespective of Cycle, in Synchronised Ewes During Non-Breeding Season
(Wiley, 2025) Cetin, Nebi; Koca, Davut
Improvement of yield characteristics in animal breeding is important in terms of increasing animal production and sustainability. Fertility is one of the most important yield traits affecting economic gain in sheep breeding. Anti-M & uuml;llerian Hormone (AMH) is widely recognised as a dependable biomarker for assessing ovarian reserves and fertility potential. The aim of this study was to evaluate the dynamics of AMH during different phases of the sexual cycle in Norduz ewes the non-breeding season. Additionally, the study sought to assess the effects of age and body condition score (BCS) on AMH concentrations during these phases. A total of 32 Norduz ewes with a body condition score (BCS) of 3-4.5 and aged between 2 and 4 years were used as animal material in the study. All experimental procedures were carried out outside the breeding season and when the ewes were lactating. In all ewes in anestrus, intravaginal sponges (Esponjavet, 60 mg MAP, Hipra, Turkey) were kept in the vagina for 7 days for estrus synchronisation. Intramuscular injections of PMSG (Oviser, 500 IU, Hipra, Turkey) and PGF2 alpha analog (Gestavet, 50 mu g, Hipra, Turkey) were administered 48 h prior to sponge removal. Twenty-four hours after sponge removal, ewes were exposed to the ram for estrus detection. Since 5 ewes did not show estrus, blood samples were collected regularly from animals (n = 27) in which estrus was detected at three different stages: one just before the insertion of vaginal sponges (anestrus), another when heat was detected exposing to the ram (estrus), and the final one 10 days after estrus (diestrus). The serum samples were assessed for the levels of AMH and progesterone through the electrochemiluminessence immunoassay technique (ECLIA). The results of the analyses showed that serum AMH concentration did not vary between anestrus, estrus and diestrus phases of the sexual cycle of Norduz ewes outside the breeding season (p > 0.05). Furthermore, age and BCS had no effect on progesterone and AMH levels in different phases of the sexual cycle (p > 0.05). In conclusion, this study shows that serum AMH levels are constant at any stage of the estrus cycle. This suggests that phenotypic evaluation of ewes can be performed with a single measurement and that AMH is a reproducible and dependable biomarker that can be measured at any stage of the estrus cycle at an arbitrary time point.
Dynamics of Oxidants, Antioxidants and Hormones During Different Phases of Pregnancy in Hairy Goats
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Cetin, Nebi; Eski, Funda; Mis, Leyla; Naseer, Zahid; Bolacali, Memis
The aim of the present study was to observe the variation in oxidant (MDA), antioxidants (SOD, GSH, GSH-Px) and hormones (P4 and E2) levels in pregnant hairy goats during breeding season. In the this study, twenty hairy goats were synchronized by using sponges containing progesterone (fluorogestone acetate). The animals showing oestrus were inseminated twice, first at 18th -24th h and second at 36th - 48th h of oestrus. On 35th and 42nd days after insemination, pregnancy diagnosis examination was performed with transrectal ultrasonography. The blood samples were collected from pregnant goats at 0,11, 24, 57, 100, 134, 141 days of gestation and immediately after the parturation. Serum samples collected at estrus and different stages of gestation were analyzed for MDA, SOD, GSH-Px, GSH, P4 and E2 concentrations using standard protocols. The results showed that MDA level did not change in pregnant goats. The SOD, GSH and GSH-Px levels also shown a similar pattern throughout pregnancy period. The peak (P<0.05) level of progesterone was recorded between 11th to 134th days of gestation. At time of estrus (day 0) and late gestation (day 134 and 141), the concentrations estradiol reached at maximal level (P<0.05) in hairy goats. In conclusion, the oxidants and antoixidants do not change with respect to dynamics of progesterone or estradiol level during gestation period in black hairy goats.
Effect of Barium Selenate Injections on Fertility of Pirlak Ewes Subjected To Estrus Synchronization During Non-Breeding Season
(Polish Soc veterinary Sciences Editorial office, 2017) Kuru, Mushap; Sogukpinar, Osman; Makav, Mustafa; Cetin, Nebi
This study was aimed at determining the effect of barium selenate injections on the fertility of Pirlak ewes which had been subjected to progesterone-assisted estrus synchronization during the non-breeding season. A total of 150 Pirlak ewes between the ages of 2 and 5 years were used in the study. Sponges containing progesterone were inserted into the vaginas of the ewes for 11 (groups I and III) or 14 days (groups II and IV) for the purpose of estrus synchronization, and 500 IU equine chorionic gonadotropin (eCG) was administered on the day of sponge removal. In addition, barium selenate was injected s.c. immediately before estrus synchronization protocol in groups I and III. After the sponges had been removed, estrus was observed for four days. The ewes were mated with fertile rams. The pregnancy was determined by transrectal ultrasonography 30 days after mating. It was observed that the estrus rate, pregnancy rate, conception rate, lambing rate, twinning rate, and litter size were not statistically different (P > 0.05) between the groups, but estrus onset was different (P < 0.001). In conclusion, barium selenate injections did not affect the fertility parameters before progesterone-assisted synchronization was applied to the Pirlak ewes during the non-breeding season.
The Effect of Hcg Administration on Reproductive Performance in Undernourished Lactating Hair Goats Synchronized During Non-Breeding Season
(veterinarni A Farmaceuticka Univerzita Brno, 2024) Cetin, Nebi; Kosal, Volkan; Kuru, Mushap; Oner, Ahmet Cihat; Eski, Funda
This study aimed to assess the effects of short and long synchronization protocols, combined with post-synchronization human chorionic gonadotropin (hCG) administration, on the reproductive performance of lactating hair goats during the non-breeding season, considering their inadequate pasture conditions. A total of 60 goats were randomly divided into four groups used for 5 days in G1 and G2 and 12 days in G3 and G4. All received a 500 IU pregnant mare's serum gonadotropin (PMSG) injection 48 h before sponge removal and were exposed to bucks 12 h later. Groups G2 and G4 received a 500 IU hCG injection on the eighth day after mating. Groups G1 and G3 did not receive any application after mating. Blood samples were collected on the 8th, 15th, and 22nd days for post-mating progesterone analysis, and pregnancy examinations were performed on the 35th day. The study showed a 90% total oestrus rate (54/60). However, there were no significant differences in conception, pregnancy, and kidding rates among the groups. Serum progesterone concentrations significantly increased on the 15th day in G2 and G4, where hCG was administered. In summary, hCG raised progesterone levels but did not significantly affect the reproductive performance of undernourished, lactating goats in a nonbreeding season, suggesting that environmental factors and animal nutrition play a crucial role in synchronization outcomes.
Effect of Oestrus Synchronization With Different Lengths of Progesterone-Impregnated Sponges and Equine Chorionic Gonadotropin on Reproductive Efficiency in Romanov Ewes During the Non-Breeding Season
(veterinarni A Farmaceuticka Univerzita Brno, 2022) Kuru, Mushap; Kuru, Buket Boga; Kacar, Cihan; Demir, Murat Can; Cetin, Nebi
This study aimed to determine the effects of different lengths of progesterone and equine chorionic gonadotropin (eCG) treatment on reproductive performance during the non-breeding season in ewes. Progesterone-impregnated sponges were inserted intravaginally for 7 d in Group 1 (G1, n = 24), 9 d in Group 2 (G2, n = 25), and 11 d in Group 3 (G3, n = 24). On the day of sponge removal, eCG (350 IU) was injected and 24 h later the ewes were exposed to rams. Pregnancy was diagnosed by transrectal ultrasonography 30 ?? 3 d after mating. Oestrus symptoms of G2 and G3 started earlier than G1, but there was only a significant difference between G1 and G3 (P = 0.013). In G1, G2, and G3, the oestrus responses were 100%, 100%, and 95.8%, pregnancy rates were 79.2%, 72%, and 75%, lambing rates were 79.8%, 72%, and 75%, litter sizes were 2.5, 2.2, and 2.4, and multiple birth rates were 86.7%, 80%, and 85.7%, respectively (P > 0.05). In conclusion, the short-term progesterone-impregnated sponge and eCG treatment provided excellent oestrus induction. The effects of different days (7, 9, or 11) of progesterone treatment on reproductive indicators were similar; therefore, the stress caused by the inserted sponge can be reduced by short-term use.
Effect of Two Prostaglandin F2α Injections Administered 24 Hours Apart on the Pregnancy Rate of Simmental Cows Subjected To the Ovsynch or Ovsynch Plus Controlled Internal Drug Release (Cidr) Protocols
(Polish Soc veterinary Sciences Editorial office, 2020) Kuru, Mushap; Kacar, Cihan; Oral, Hasan; Kaya, Semra; Cetin, Nebi; Kaya, Duygu; Demir, Murat Can
The aim of the present study was to determine the effects of one or two doses of prostaglandin F2 alpha (PGF) on the pregnancy rate (PR) in Simmental cows subjected to the Ovsynch or Ovsynch + Controlled internal drug release (CIDR) protocols. On d 0, 100 mu g gonadorelin diacetate tetrahydrate (GnRH) was injected into cows in group 1 (n = 50) and group 2 (n = 40) and CIDR was intravaginally inserted. On d 7, 25 mg dinoprost tromethamine (PGF2 alpha, PGF) was administered, and GnRH was injected 56 h later. Fixed-time artificial insemination (TAI) was performed 16 h after GnRH injection. In group 3 (n = 62) and group 4 (n = 63), PGF was administered 7 d following Gn RH treatment, and GnRH was injected 56 h later. TAI was performed 16 h after Gn RH injection. In groups 2 and 4, a second dose of PGF was injected 24 h after the first dose. Pregnancy was determined by transrectal ultrasonography on d 30 and 60 post-TAI. The pregnancy rate (PR) on d 30 post-TAI was significantly different among the groups: 46%, 55%, 29%, and 36.5%, in groups 1, 2, 3, and 4, respectively (P = 0.048). In addition, the PR was 36.3% and 43.7% in the single PGF and double PGF injection groups, respectively. There was no statistical difference in the PR on d 60 post-TAI (P > 0.05). Pregnancy loss was between 4.34% and 11.11% (P > 0.05). The highest PR in the prim iparous cows was observed in group 3 (57.7%). The PR was not significantly different among multiparous cows (P> 0.05). In conclusion, two injections of PGF administered 24 h apart in Simmental cows may increase the PR in the Ovsynch or Ovsynch + CIDR protocols.
Effects of Long-Term Release Gnrh Agonist "deslorelin" on Testicular Hsp Expression, Accessory Sex Glands and Testicular Functions in Adult Male Rats
(Elsevier Science inc, 2019) Eski, Funda; Cetin, Nebi; Uslu, Sema; Uslu, Baris Atalay; Sendag, Sait; Yoruk, Mecit; Shakeel, Muhammad
The objective of the present was to determine the effect of long-term release GnRH agonists "deslorelin" on suppression and restoration of testicular and accessory sex glands functions, and expression of HSP in testes of adult male rats. A group of twenty-eight male rats and fifty-six female rats were kept for eleven months. The male rats were subdivided into treatment (n = 18; deslorelin, an analogue of GnRH, 4.7 mg, S.C; six months) and control (n = 10; untreated), and the adult female rats were introduced with either treatment or control male rats at the 2nd, 6th and 11th months post implant insertion. At 6th month of deslorelin implants insertion, six male rats from treatment and five rats from control group were sacrificed. The remaining (twelve treatment and five control) male rats were sacrificed at 11 months. The testicular dimension were measured monthly in both treatment and control rats. The blood samples were collected for testosterone and HSP70 antibody, whereas, the testes and accessory glands were isolated for histological examination at each sacrificial time. The results showed that testicular dimension were significantly lesser in treatment group until 9 months post treatment. HSP70 protein expression was negligible at 6 months in treatment group but its intensity increased in spermatids 11 months of treatment similar to control group. Significantly lower testosterone concentrations with poor semen quality, and smaller litter size were observed in treatment group. The histological picture of accessory sex glands and seminiferous tubules shown a variable integrity in treatment group than control at 6 months implant insertion. In conclusion, the subcutaneous application of 4.7 mg of the GnRH-analogue deslorelin represents a practicable, like in the female rats, method to suppress testicular, accessory sex glands functions, testicular HSP expression and fertility in male rats. Moreover, the suppressive effects of deslorelin, continued until 11th months after removal of the implant. (C) 2019 Elsevier Inc. All rights reserved.
Mining for the Association of Bovine Mastitis Linked Genes To Pathological Signatures and Pathways
(Walter de Gruyter Gmbh, 2022) Khan, Muhammad Zahoor; Belhan, Saadet; Cetin, Nebi; Ayan, Adnan; Khan, Adnan; Ahmad, Irshad; Cao, Zhijun
Background: Bovine mastitis is a common infectious disease with a serious threat to the dairy industry and public health. Mastitis is a polygenetic trait under the control of many genes. In the current study, our research attempted to address the role of mastitis-associated genes in various signalings including parasitic, viral, cancer and fungal diseases by using online bioinformatics software. Methods: We selected mastitis-associated genes from already published data and using online bioinformatics tools including DAVID and String classify the pathological role of relevant genes. A Venn diagram was used to show the status of overlapping genes among different biological function processes. Result: This study revealed that the genes gathered in published resources of mastitis were significantly correlated with Influenza A, Chagas disease, Leishmaniasis, Toxoplasmosis, Tuberculosis, Cancer signaling, Hepatitis B, Type I &II diabetes mellitus and Prion diseases biological pathways. Based on our findings, we concluded that mastitis-linked genes could be used as markers for many other diseases. Moreover, the Bioinformatics tools applied in the current study might be helpful in screening the genes involved in one disease and their association with other diseases as well.