Browsing by Author "Cetin, S."

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The Correlation Between Neopterin, Myeloperoxidase and Oxidative DNA Damage in Sheep With Natural Babesiosis
(Selcuk Universitesi, Veteriner Fakultesi, 2024) Cetin, S.; Komuroglu, A.U.; Yuksek, V.; Dede, S.; Kilinc, O.O.; Yuksek, N.
Aim: The present study was designed to investigate the correlation between neopterin, myeloperoxidase (MPO), and DNA damage in sheep naturally infected with babesiosis. Materials and Methods: The study included 40 sheep Akkaraman, 1-3 years aged, with 20 healthy individuals (control group) and 20 naturally infected with babesiosis (patient group). Blood samples were collected from the jugular vein into biochemical tubes from all sheep. The cause of the disease was confirmed through smear tests and PCR analysis. Neopterin, myeloperoxidase (MPO), and oxidative DNA damage (8-OHdG) levels were measured using an ELISA method. Results: Neopterin and 8-OHdG levels were significantly lower in the control group compared to the patient group (P<0.05). Although myeloperoxidase were higher in the patient group, the difference was not statistically significant (P>0.05) Conclusion: In this study, a significant increase in neopterin and oxidative DNA damage, which are indicators of an immunological response, was observed in sheep with babesiosis. Neopterin in particular can be considered as an important marker of the immunological response to babesiosis. © 2024 Selcuk Universitesi, Veteriner Fakultesi. All rights reserved.
Effect of Lycopene on Serum Nitrite-Nitrate Levels in Diabetic Rats
(Medknow Publications & Media Pvt Ltd, 2015) Yegin, Sevim C.; Yur, Fatmagul; Cetin, S.; Guder, A.
The effect of lycopene on serum nitrate-nitrite levels was investigated in diabetic rats. In this investigation, 28 Wistar rats were divided into 4 groups, each of seven rats. These groups were control group, diabetes group, diabetes-lycopene group and lycopene group. The concentration of nitrite and nitrate was detected at high levels in diabetes group, diabetes-lycopene group and lycopene group as compared with the control group (P < 0.05). Especially, the increase in the levels of nitrate in diabetes group and lycopene group was statistically significant when compared with diabetes-lycopene group and control group (P < 0.05). In addition, we also determined the proportion of nitrite/nitrate for nitric oxide radical formation. Therefore, it is important to investigate the recovery and stability of nitrite and nitrate in samples. As a result of this study, it was observed that the amounts of nitrate and nitrite increased due to oxidative stress in diabetes and also application of antioxidant lycopene caused an increase in the amounts of nitrate and nitrite levels.
Effects of the Replacement of Soybean Meal With Pea as Dietary Protein Source on the Serum Protein Fractions of Broilers
(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2016) Bingol, N. T.; Dede, S.; Karsli, M. A.; Deger, Y.; Kilinc, Kilicalp D.; Cetin, S.
The aim of this study was to determine the effects of the replacement of different levels of protein derived from soybean meal with that from peas in broiler diets on serum protein fractions. A corn-soybean meal basal diet was formulated as the control diet (Control=C) (NRC, 1994), and then pea was added to the control diet to replace 20% (P20) or 40% (P40) of the crude protein of the control diet. The diets were randomly fed to 12 pens per treatment, each housing five birds, for 42 days. Blood samples were collected from 36 birds (3 birds x 4 pens x3 treatments) and the serum protein fractions were separated. Gamma-globulin percentage was higher in group P20 compared with C and P40 groups. Total protein, beta-globulin, and gamma-globulin concentrations were significantly higher in group P20 compared with those of both control and P40 group (p<0.05).
Expression Levels of Some Apoptotic and Oxidative Genes in Sheep With Sarcocystosis
(Hellenic veterinary Medical Soc, 2022) Yuksek, V.; Kilinc, O. O.; Dede, S.; Cetin, S.; Ayan, A.
Sarcocystosis is a zoonotic protozoon-related disease with a very broad intermediate host spectrum. These protozoon parasites lead to tissue loss in their intermediate hosts. The purpose of this study was to present the mRNA expression levels of some genes belonging to the oxidative stress and apoptosis pathway systems in tissue damage caused by sarcocystosis. In this study, the material consisted of infected tissue taken from sheep esophagus determined to be sarcocystosis-infected and esophageal tissues taken from healthy sheep. The expression levels of the GPX1, SOD1, SOD2, NCF1and Nos2 genes that play a role in the oxidative stress mechanism and the caspase 3, 8, 9 and BCL-2 genes that play a role in the apoptosis mechanism were determined by RT-qPCR. As a result of the study, it was determined that, with increased oxidative stress, the gene expressions related to the relevant enzyme systems also increased, and in relation to this increase, the caspase enzyme genes that are effective in cell death were up-regulated. These results may shed light on similar studies for understanding and preventing damage mechanisms that may form as a result of sarcocystosis. As a result, it is understood that increased oxidative stress parameters and increased apoptosis in sarcocystic tissue in sheep cause tissue loss. We think that understanding the molecular mechanisms of this disease is clinically important in the treatment of parasitic diseases and in the prevention of economic losses that may occur as a result of the disease.
In Vitro Evaluation of Glutathione Implementation on Oxidative DNA Damage and Oxidant Status in High Glucose Conditions
(Selcuk Universitesi, Veteriner Fakultesi, 2022) Yur, F.; Dede, S.; Cetin, S.; Taspinar, M.; Usta, A.
Aim: This study aimed to show the effects of glutathione, recognized by its antioxidant specialties, on the potential DNA damage (8-hydroxy-2-deoxyguanosine) and the antioxidant system changes upon its implementation in BHK-21 cells cultured with high glucose. Materials and Methods: BHK-21 cell line was regularly surpassed in vitro conditions (5% FBS, 10% horse serum, 1% L-Glutamine, 1% penicillin/ streptomycin in RPMI 1640 medium, and 5% CO2 and 95% humidity and 37oC) incubated. The control group determined glucose's IC50 value based on the viability tests executed on MTT cells. Cells were seeded in plates as each would have 2x106 cells. The control, the test, and the crossbreed test (glucose; (285 mM), glutathione (250 µM)) groups were prepared. After 24 hours of incubation, trypsinized cells were designed for analysis through vitrification. In the lysate of the cell culture that was procured, Oxidative DNA damage, TAS, TSO, and OSI were measured by the spectrophotometric system with ELISA. Results: It was observed that 8-OHdG levels increased significantly with glucose application. Moreover, the increase in the HG+GSH group was more significant when compared to the control group (p≤0.05). No difference with the control group was found only in the group where GSH was applied. As for TAS, whereas any difference was observed in GSH used groups, the increase in the HG+GSH group was significant compared to the control group (p≤0.05). that were the same as the control group. TOS and OSI considerably increased in HG + GSH implemented groups as to the control group (p≤0.05). Conclusion: According to the results, no protective impacts of glutathione at the cellular level in the doses mentioned above were observed on high-dose glucose implemented cells. On the other hand, it was revealed that the applied amounts of glutathione in the process did not cause any toxic effects. © 2022 Selcuk Universitesi, Veteriner Fakultesi. All Rights Reserved.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride
(Humana Press inc, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.
Prolonged exposure to high doses of fluoride causes chronic poisoning called fluorosis, which affects many tissues and causes serious health problems. This study was planned to investigate the apoptotic, autophagic, and necrotic molecular pathways of fluoride. Sodium fluoride (NaF) was administered to normal rat kidney epithelial (NRK-52E) cells. The NaF IC50 value was determined using the MTT assay. The expression of the genes in the autophagic, apoptotic, and necrotic pathways was determined by real-time PCR. It was determined that there were significant changes in NaF-induced molecular pathways depending on the time. There were no increases in apoptotic and necrotic pathway markers except for Atg3, an autophagy gene, at the 3rd and the 12th hours. However, there was an induction in all cell death signaling pathways at 24 h. The molecular mechanisms demonstrated NaF-induced cellular death in the NRK-52E cell line. It was concluded that these molecular mechanisms were activated with NaF, and different mechanisms accelerated the cellular death at the 24th hour.
In Vitro Evaluation of the Apoptotic, Autophagic, and Necrotic Molecular Pathways of Fluoride (Nov, 2020, 10.1007/S12011-020-02491-3)
(Springernature, 2021) Urut, F.; Dede, S.; Yuksek, V.; Cetin, S.; Usta, A.; Taspinar, M.