Browsing by Author "Cibuk, Salih"

Now showing 1 - 12 of 12

Advanced Oxidation Protein Product (Aopp) Levels in Second- and Third-Degree Thermal Burns
(Oxford Univ Press, 2021) Mert, Handan; Acikkol, Suat; Calli, Iskan; Cibuk, Salih; Keskin, Siddik; Mert, Nihat
Burn is a systemic injury affecting the entire organism according to its etiology and severity. The aim of this study was to investigate plasma AOPP levels before and after treatment of second- and third-degree thermal burn patients and determine the changes in this parameter, and also, to find out the relationship between AOPP level and hospitalization period and total body surface area (TBSA). The study material consisted of pediatric patients with the complaint of second- and third-degree thermal burns aged between 1 and 18 years, with a burn area exceeding 10%. Blood samples were taken twice before and after treatment. AOPP level in blood plasma was measured in ELISA. It was observed that in the second-degree thermal burn group, AOPP level was 25.85 +/- 2.82 ng/ml before the treatment decreased to 22.16 +/- 3.62 ng/ml after treatment, whereas in the third-degree thermal burn group before the treatment AOPP was 25.96 +/- 3.49 ng/ml, and after the treatment dropped to 21.70 +/- 3.79 ng/ml, decreases were significantly important (P < .05). There was no statistically significant difference between the two groups in terms of AOPP levels (P > .05). Correlation analyses in the second- and third-degree thermal burn group did not show any correlation between AOPP levels and burn area and length of hospitalization period. As a result, AOPP level has been studied, for the first time, in burn cases. In both groups, the level of AOPP increased due to oxidative stress before treatment and decreased after treatment.
Antidiabetic Effect of Fig Seed Oil in Rats With Diabetes Induced by Streptozotocin
(Japan Oil Chemists Soc, 2024) Mert, Handan; Mert, Nihan; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
The anti -diabetic effect of Ficus carica (Fig) seed oil was investigated. 4 groups with 6 rats in each group were used in the experiment as control, diabetes (45 mg/kg streptozotocin), fig seed oil (FSO) (6 mL/ kg/day/rat by gavage) and diabetes+FSO groups. Glucose, urea, creatinine, ALT, AST, GSH, AOPP and MDA analyses were done. Pancreatic tissues were examined histopathologically. When fig seed oil was given to the diabetic group, the blood glucose level decreased. In the diabetes+FSO group, serum urea, creatinine, AOPP, MDA levels and ALT and AST activities decreased statistically significantly compared to the diabetes group, while GSH levels increased significantly, histopathological, immunohistochemical, and immunofluorescent improvements were observed. It has been shown for the first time that FSO has positive effects on blood glucose level and pancreatic health. It can be said that the protective effect of fig seed oil on tissues may be due to its antioxidant activity.
Antioxidant Effect of Chromium Picolinate on Chronic Exercise-Induced Oxidative Stress in Male Rats
(Nature Portfolio, 2025) Cibuk, Salih; Sarikaya, Mucahit; Ulema, Mustafa Sencer; Pancar, Zarife; Oge, Beyza; Embiyaoglu, Nuri Mert; Batrakoulis, Alexios
Chromium picolinate influences antioxidant mechanisms, potentially affecting oxidative stress levels during prolonged aerobic exercise. This study investigates the effects of chromium supplementation on Catalase (CAT) activity and Malondialdehyde (MDA), advanced oxidation protein products (AOPP), and reduced glutathione (GSH) levels in chronic aerobic exercise in male rats. Twenty-eight male Wistar albino rats were divided into 4 groups containing 7 mice each (control, exercise, chromium, and chromium + exercise). Supplemented rats received chromium picolinate (8 mu g/kg/day) daily for eight weeks. Exercise was performed on a rat treadmill at an average speed of 15 cm/s for 20 min, five days a week for eight weeks. At the end of the 8th week of the experimental period, blood samples were taken. CAT, MDA, AOPP, and GSH analyses were performed. It was observed that the chromium + exercise group induced a significant reduction in CAT activity compared to the other three groups (- 8.6 to - 12%, p < 0.05). MDA values meaningfully increased (18.2-25.7%, p < 0.001) in all groups, except the controls after the 8-week intervention. All groups demonstrated an increase in AOPP (8.1-12.3%, p < 0.001), but not the controls. In GSH, all experimental groups showed a significant elevation (30.3-45.8%, p < 0.001) compared to the control group (p < 0.001) following an 8-week intervention period. The present findings indicate that supplementation with chromium picolinate, whether administered alone or in conjunction with aerobic exercise, led to modulations in oxidative stress and redox status indices in male rats following an 8-week aerobic exercise regimen. The observed reduction in CAT activity may suggest a lowered oxidative challenge; however, this finding should be interpreted with caution, as decreased antioxidant enzyme activity can also reflect a potential limitation in defense capacity.
Determination of Total Lipid and Fatty Acid Composition of Pearl Mullet (Chalcalburnus Tarichi, Pallas 1811)
(Central Fisheries Research inst, 2013) Misir, Gulsum Balcik; Kutlu, Sebahattin; Cibuk, Salih
Total lipid content and fatty acid composition of pearl mullet, which is an endemic species occurring in Lake Van, Eastern Anatolia, Turkey, and the streams feeding the Lake, were investigated. Average total lipid content of samples collected in September and November 2012 and January 2013 was 2.19g. Average Sigma SFA, Sigma MUFA and Sigma PUFA were determined as 21.88% (0.41g/100g), 44.37% (0.85g/100g) and 29.19% (0.57g/100g), respectively. Predominant SFA and MUFA were Palmitic acid (16: 0) 12.9% (0.25g/100g) and Oleic acid (18: 1n-9) 27.2% (0.53g/100g) in order. Average Sigma n-3 and DHA+EPA values were calculated as 23.88% (0.46g/100g), and 17.07% (0.33g/100g) respectively. Recommended as a good index in revealing the nutritional value of fish, n-3/n-6 ratio was calculated as 3.70g/100g in average. As a result, possessing especially high values of DHA and EPA, pearl mullet found to be a rich source of n-3.
The Effect of Evening Primrose Oil (Oenothera Biennis) on the Level of Adiponectin and Some Biochemical Parameters in Rats With Fructose Induced Metabolic Syndrome
(Taylor & Francis Ltd, 2022) Mert, Handan; Irak, Kivanc; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
The effect of evening primrose oil on adiponectin level and some biochemical parameters in model of fructose-induced metabolic syndrome were investigated. The rats were divided into 4 groups: control, evening primrose oil, fructose, fructose + evening primrose oil. Body weight, daily feed and water consumptions and systolic blood pressures of animals were measured. At the end of trial, blood samples were taken, livers were excised and histopathological examination was performed. Glucose, uric acid, triglyceride, T.cholesterol, LDL, HDL, VLDL, ALT, AST, ALP, LDH, adiponectin, insulin, IL-6, TNF-alpha, TAC, and TOS levels were analysed. Some analysed parameters and systolic blood pressure of fructose + evening primrose oil group decreased significantly compared to fructose group and adiponectin, TAC, and HDL levels were significantly increased. As conclusion, evening primrose oil can be considered as antioxidant agent by reducing oxidative stress, increasing adiponectin levels and insulin sensitivity, anti-inflammatory properties, exhibiting anti-atherogenic effect by regulating dyslipidemia and systolic blood pressure.
The Effect of Protocatechuic Acid on Nephrotoxicity Induced by Gentamicin in Rats
(Springer, 2025) Mert, Handan; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
Gentamicin (GM) is an aminoglycoside antibiotic widely used to treat gram-negative infections. Oxidative stress is known to play an important role in the nephrotoxicity of gentamicin. Therefore, the aim of this study was to investigate the possible protective effect of protocatechuic acid (PCA), which is believed to have antioxidant properties, on nephrotoxicity induced by gentamicin. For this purpose, 32 rats were randomly divided into four groups: control (oral physiological saline), PCA (20 mg/kg orally), GM (80 mg/kg/day/i.p.), GM+PCA (80 mg/kg/day/i.p. GM and 20 mg/kg PCA orally). The sampling period was eight days. Blood samples were collected for biochemical analysis and kidney samples for immunohistochemical and histopathological examination. Serum levels of urea, creatinine, Na, K and Cl were measured using an autoanalyzer, while analyses of malondialdehyde (MDA), advanced oxidation protein products (AOPP), gutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were analyzed by ELISA. While the values of urea (p<0.001), creatinine (p<0.001), MDA (p<0.05) and AOPP (p<0.05) decreased in the GM+PCA group compared to the GM group, the values of GSH (p<0.05) and GPx activity (p<0.05) increased. In conclusion, in GM-induced nephrotoxicity, PCA prevented lipid peroxidation and protein oxidation, increased GSH levels and GPx activity, and reduced tubular epithelial necrosis, glomerular atrophy, 8-OHdG and Kim-1 expression in renal cells, according to histopathological and immunohistochemical results. This study once again highlighted that PCA is a good antioxidant, and it can be said that PCA has a protective effect against nephrotoxicity caused by GM.
The Effect of Royal Jelly on Levels of Tnf-Alpha and Oxidant-Antioxidant Systems in Experimental Rats Hypothyroidism
(Parlar Scientific Publications (p S P), 2020) Cibuk, Salih; Mert, Handan; Aksu, Devrim Saripinar; Mert, Nihat
Royal jelly is a product of bees and contains proteins, carbohydrates, fats, free amino acids, vitamins and minerals. In this study, the effect of royal jelly on TNF-alpha levels and oxidant-antioxidant systems in hypothyroid rats induced by propylthiouracil (PTU) were investigated. Forty rats were randomly divided into four groups each containing 10 rats as control, royal jelly, PTU and PTU + royal jelly groups. At the end of the experiment, hematological parameters, sT3, sT4 and serum lipid profile, levels of TSH, TNF-alpha, GSH, MDA, GSH-Px, SOD and CAT were determined. The levels of sT3 (p < 0.001) and sT4 (p < 0.001) were significantly decreased and TSH levels (p < 0.05) increased in PTU and PTU + royal jelly groups compared to control group. There was a decrease in triglyceride levels (p < 0.05) and an increase in LDL (p < 0.01), RBC (p < 0.05), HCT (p < 0.01) and HGB (p < 0.01) in PTU + royal jelly group compared to the PTU group. Levels of TNF-alpha, GSH and GSH-Px, CAT, SOD were not significantly different between groups. As a result, increase in oxidative stress was not detected in hypothyroid rats induced by PTU. Therefore we could not observe the effect of royal jelly on oxidative stress. In hypothyroidism, royal jelly had positive effects on triglycerides, RBC, HCT and HGB. Especially for the treatment of anemia in hypothyroidism royal jelly may be recommended. This is a preliminary study that shows the effect of royal jelly on hypothyroidism. There is a need for further studies on this issue.
Effect of X-Ray Exposure on Oxidative Stress in Liver and Kidney in Rats in Early Life: an Experimental Study
(Kafkas Univ, Veteriner Fakultesi Dergisi, 2025) Cibuk, Salih; Aras, Abbas
The aim of this study was to investigate the levels of oxidative stress and antioxidants in the liver and kidney tissue of baby rats exposed to whole-body x-ray by creating a newborn rat model. In this study, 60 baby rats obtained from 15 pregnant rats were used. Pregnant rats were randomly divided into five groups. The control group (Group I) was not subjected to X-ray. The 2(nd) and 3(rd) groups were subjected to both intrauterine and postnatal X-ray, and the 4(th) and 5(th) groups were subjected to only postnatal X-ray. At the end of the 4-week study period, oxidative stress markers were studied in the liver and kidney tissue. In all groups that received X-ray, an increase in the amounts of malondialdehyde (MDA) and advanced oxidation protein products (AOPP), a decrease in the amount of glutathione (GSH) and catalase (CAT) activity were detected in liver tissues (P<0.05), and an increase in the activities of MDA, AOPP and CAT, and a decrease in the amount of GSH were detected in kidney tissues (P<0.05). These findings indicate that X-ray exposure in early life disrupts the antioxidant defense system by inducing oxidative stress in liver and kidney tissues, highlighting the necessity of minimizing unnecessary radiation exposure in clinical practice.
Effects of X-Ray Application on Infertility in New-Born Rats
(Taylor & Francis Ltd, 2023) Cibuk, Salih; Mert, Handan; Mert, Nihat; Tuncer, Oguz; Altindag, Fikret; Karaman, Kamuran; Meydan, Ismet
In this study, the effect of early X-ray exposure on infertility was investigated by creating a newborn model with rats. Fifteen Pregnant rats were divided into five groups. After birth, the study was continued with 12 babies (6 males, 6 females) rat in each group. Different amounts of X-rays were applied to the experimental groups. At the end of the experiment, there was found that testosterone levels decreased in all experimental groups compared to the control group (P < 0.05). When the experimental groups were compared to the control group, there was a decrease in the number of spermatogoniums from all the experimental groups. The decrease in group II, group III and group IV was found to be statistically significant (P < 0.05). As a result, exposure to X-rays in new-borns and premature babies; It was observed that it caused disruption of caspase signaling in gonad organs, a serious decrease in hormonal activity, a significant decrease in spermatogonia number and a decrease in the number of primordial follicles. Considering these results, it can be predicted that exposure to X-rays in the neonatal period, especially in the premature period, may lead to infertility in later life.
Evaluation of Effectiveness of Neutral-Ph Superoxidized Solution (Nsos) With Peritoneal Lavage in Rat Fecal Peritonitis Model: an Experimental Study
(Kafkas Univ, veteriner Fakultesi dergisi, 2024) Aras, Abbas; Karaman, Erbil; Seckin, Hamdullah; Cibuk, Salih
This experimental study aimed to evaluate the effectiveness of NSOS (Neutral-pH superoxidized solution) with peritoneal lavage in rat fecal peritonitis models. Forty Wistar rats weighing between 250-300 g were used for the study. All rats were inducted for fecal peritonitis formation. The rats were divided into five groups as follows; Group 0: control, no intervention. Group 1: Application of 10 mg/kg NSOS into the peritoneal cavity after 6 h of induction of peritonitis. Group 2: 10 mg/kg NSOS application and repeated at 24 and 48 h. Group 3: NSOS + Antibiotic treatment (Seftriakson 30 mg/kg/ day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). Group 4: Only antibiotic treatment (Seftriakson 30 mg/kg/day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). The animals were examined for peritoneal and thoracal abscess formation, adherences, and any abnormality with inspection after sacrificing on the 7th day. The peritoneal lavage fluid culture for microbiological analysis and blood samples were taken for blood cultures, biochemical and infectious parameters of WBC, CRP, TNF- alpha, IL-6, IL-1 beta, and IL-10. Peritonitis was developed in all rats at the end of follow-up. No death was observed in rats on the seventh day of the experiment. Group 3 (NSOS + Antibiotic treatment) showed the most significant improvement in the infection of peritoneal fluid. NSOS and antibiotic together (Group 3) were found to be more effective against Klebsiella than Enterococcus sp. The blood cultures showed a significant reduction in all groups. The infectious parameters including IL-6, IL-1 beta, and IL-10 showed no significant difference in the first week of treatment between all groups. Only TNF- alpha was observed significantly lower in group 3 when compared to the other groups (P=0.001). Peritoneal lavage with neutral pH-superoxidized water plus an antibiotic regimen is the most effective treatment in the rat fecal peritonitis model. Further studies including human subjects are needed to investigate its effectiveness and validity
Possible Relationships Between Placenta and Serum Asprosin Levels and Pregnancy and Spontaneous Preterm Births
(BMC, 2025) Gul, Cihan; Uckan, Kazim; Cibuk, Salih
ObjectiveAsprosin, a newly characterised adipokine, has been implicated in several physiological and pathological pathways. This study aimed to explore the association between serum and placental asprosin concentrations and the occurrence of preterm births by evaluating their potential as predictive biomarkers.MethodsA total of 75 participants were enrolled in this cross-sectional study and categorised into four groups - early preterm (delivery before 34 weeks, n = 15), late preterm (34-37 weeks, n = 15), term delivery (37-42 weeks, n = 30) and nonpregnant controls (n = 15). Serum samples were collected from all individuals, and placental tissues were obtained postdelivery from pregnant participants. Asprosin concentrations were quantified using ELISA, and correlation analyses were conducted to determine associations with clinical variables.ResultsSignificantly elevated asprosin levels were detected in both the serum and placental samples of women with preterm births compared to term deliveries (p = 0.008 for placental samples, p = 0.001 for serum samples). The highest levels were noted in the early preterm group (placental: 18.88 +/- 2.12 ng/ml; serum: 19.04 +/- 3.15 ng/ml). Strong inverse correlations were identified between asprosin levels and gestational age (placental: r = -0.647, p = 0.01; serum: r = -0.716, p = 0.01) and between serum asprosin and neonatal birth weight (r = -0.683, p = 0.01). The ROC analysis indicated cutoff values of 16.58 ng/ml (placenta) and 15.22 ng/ml (serum) as potential thresholds for preterm birth prediction.ConclusionIncreased maternal serum and placental asprosin levels are linked to preterm birth, demonstrating inverse associations with gestational duration and infant birth weight. These results suggest a potential role for asprosin as a predictive biomarker for preterm birth, warranting further mechanistic investigation.
Severity of Mitral Valve Stenosis ― Possible Relationships With Blood Oxidant Markers and Antioxidants ―
(Japanese Circulation Soc, 2024) Duz, Ramazan; Cibuk, Salih
Background: This study examined whether the severity of mitral valve stenosis (MVS) is associated with oxidative stress (OS) markers in the blood, and other hematological and clinicodemographic parameters. Methods and Results: This prospective study was conducted between March and May 2022. Seventy-five patients with newly diagnosed MVS (25 mild, 25 moderate, 25 severe) were included. Mild, moderate, and severe MVS was defined as MV area >2, 1.5-2, and <1.5 cm(2), respectively. Various OS markers and laboratory parameters were determined in venous blood samples. For predictive analyses, 2 different analyses were performed to detect patients with severe MVS and those with moderate or severe (moderate/severe) MVS. Age (P=0.388) and sex (P=0.372) distribution were similar in the 3 groups. Multiple logistic regression analysis revealed that a high white blood cell (WBC) count (P=0.023) and high malondialdehyde (P=0.010), superoxide dismutase (SOD; P=0.008), and advanced oxidation protein products (AOPP; P=0.007) levels were independently associated with severe MVS. A low platelet count (P=0.030) and high malondialdehyde (P=0.018), SOD (P=0.008), and AOPP (P=0.001) levels were independently associated with having moderate/severe MVS. The best discriminatory factors for severe MVS were SOD (cut-off >315.5 ng/mL) and glutathione (cut-off >4.7 mu mol/L). Conclusions: MVS severity seems to be affected by oxidant markers (malondialdehyde and AOPP), antioxidant enzymes (SOD), and inflammation-related cells (WBC and platelets). Future studies are needed to examine these relationships in larger populations.