Browsing by Author "Cilingir Yeltekin, Asli"

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Assesment of Hematotoxic, Oxidative and Genotoxic Damage Potentials of Fipronil in Rainbow Trout Oncorhynchus Mykiss, Walbaum
(Taylor & Francis Ltd, 2021) Ucar, Arzu; Parlak, Veysel; Cilingir Yeltekin, Asli; Ozgeris, Fatma Betul; Caglar, Ozge; Turkez, Hasan; Atamanalp, Muhammed
In this study, changes in the blood tissue of rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) caused by Fipronil (FP) insecticide were investigated using different biomarkers (Hematology parameters, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), malondialdehyde (MDA), paraoxonase (PON), arylesterase (ARE), myeleperoxidase (MPO), micronucleus (MN), 8-hydroxy-2-deoxyguanosine (8-OHdG)) level and caspase-3 activity. Statistically significant alterations in hematology parameters occurred with FP effect. In blood tissue, dose-dependent inhibition was determined in SOD-CAT-GPX-PON and ARE enzyme activities, but MDA and MPO were induced statistically significant. The results of MN assay were compared with the control group and it was obtained that genotoxicity of different dose groups was similar. The level of 8-OHdG and the activity and caspase-3 examined in blood tissue was increased depending on the dose. It was determined with different biomarkers that this insecticide caused physiological stress changes in the tissues examined.
Borax Attenuates Oxidative Stress, Inflammation, and Apoptosis by Modulating Nrf2/Ros Balance in Acrylamide-Induced Neurotoxicity in Rainbow Trout
(Taylor & Francis Ltd, 2025) Turkez, Hasan; Alak, Gonca; Ozgeris, Fatma Betul; Cilingir Yeltekin, Asli; Ucar, Arzu; Parlak, Veysel; Atamanalp, Muhammed
Acrylamide (ACR) can have adverse environmental effects because of its multiple applications. Relevant scientific literatures of the existence of ACR residues in foods following processing steps have raised concern in the biochemistry, chemistry and safety of this vinyl substance. The interest has focused on the hepatotoxicity of ACR in animals and humans and on the ACR content mitigation and its detoxification. Borax (BX), as a naturally occurring antioxidant featured boron compound, was selected in this investigation to assess its possible neuro-protective potential against ACR-induced neurotoxicity. Nrf2 axis signaling pathways and detoxification response to oxidative stress after exposure to ACR in brains of rainbow trout, and the effect of BX application on reducing ACR-induced neurotoxicity were investigated. Rainbow trout were acutely exposed to ACR (12.5 mg/L) alone or simultaneously treated with BX (0.75 mg/L) during 96h. The exposed fish were sampled at 48th and 96th and oxidative stress response endpoints, 8-OHdG, Nrf2, TNF-alpha, caspase-3, in addition to IL-6 activities and the levels of AChE and BDNF in brain tissues of rainbow trout (Oncorhynchus mykiss) were evaluated. Samples showed decreases in the levels of ACR-mediated biomarkers used to assess neural toxicity (SOD, CAT, GPx, AChE, BDNF, GSH), increased levels of MDA, MPO, DNA damage and apoptosis. ACR disrupted the Nrf2 pathway, and induced neurotoxicity. Inhibited activities' expressions under simultaneous administration experiments, revealed the protective effects of BX against ACR-induced toxicity damage. The obtained data allow the outline of early multi-parameter signaling pathways in rainbow trout
Toxic and Trace Element Levels in Salmo Trutta Macrostigma and Oncorhynchus Mykiss Trout Raised in Different Environments
(Hard, 2019) Cilingir Yeltekin, Asli; Saglamer, Emin
The increase in human population along with economic and technological developments has led to significant degrees of deterioration in water quality globally. As a result, it is important to determine the toxic metal concentrations in organisms living in water, especially in commonly consumed fish species. With this aim, due to the shallow water in our region we chose the important trout production location of the Catak River for sampling in our study, using natural Salmo trutta macrostigma and Oncorhynchus mykiss from the river and Salmo trutta macrostigma and Oncorhynchus mykiss bred in a trout production facility in Catak District. These fish had element levels (Be, B, Al, Ti, Cr, Mn, Co, Ni, Cu, Zn, Mo, Cd, Ba, Pb, Na, Mg, Si, K, Ca, Fe, As, Se, and Sb) measured in heart, muscle, liver, kidney, gills, gonads, brain, and intestinal tissue using an ICP-OES device. The results of the study are grouped as toxic (Be, Cd, Pb, Al, Ba, Ti), trace (Fe, Zn, Cu, Mn, Co, Ni, Cr, B, Si, Mo) and macro (Ca, Mg, K, Na) elements for assessment. As a result, the nutrient values of the trout grown on the farm and in the natural environment were high.