Browsing by Author "Cokluk, E."

Now showing 1 - 4 of 4

Biological Variation of Iron, Transferrin, Ferritin, Folate, Vitamin B12 and 25-Oh Vitamin D in Healthy Individuals
(Clin Lab Publ, 2021) Cokluk, E.; Akbay, Hi; Alp, Hh
Background: We aimed to evaluate the use of population-based-reference-intervals by calculating the individuality index and reference change value of iron, transferrin, ferritin, folate, vitamin B12, and 25-OH vitamin D parameters. Methods: A total of 5 venous blood samples were taken from 11 female and 10 male individuals on days 0, 7, 14, 21, and 28. All tests were performed on an autoanalyzer and CVI, CVG, II, and RCV values were calculated for both genders and the whole groups. Results: CVA (%)/CVI (%)/CVG (%) for iron, 0.67/27.3/32.3 transferrin, 0.62/3.60/10.27 ferritin, 2.27/6.21/105.6 folate 4.71/10.3/28.56 vitamin B12, 6.1/5.77/34.6, 25-OH Vitamin D 3.4/8.2/54.9 respectively. RCV calculated as a 2-tailed value at level of probability of significant change set at 0.95 - 0.99; 74.9/98.7 - 10.1/13.3 - 18.3/24.1 31.4/41.3 - 23.4/30.8 - 24.7/32.5 and II were 0.8-0.4-0.-0.4-0.17-0.1 respectively. Conclusions: CVI and CVG values for iron, transferrin, folate, vitamin B12, and 25-OH vitamin D are in accordance with the literature. Ferritin was calculated differently from the values in the database. This difference might be due to the characteristics of selected individuals for study. Evaluating the suitability and utility of the use of RR with values found for II. For iron, the use of RR was considered appropriate when taken as II < 0.6 and II > 1.4. However, the use of RCV is more appropriate for Transferrin, Folate, Vitamin B12, and 25-OH Vitamin D. (Clin. Lab. 2021;67:2048-2054. DOI: 10.7754/Clin.Lab.2021.210102)
Levels of Leukocyte Oxidative Dna Damage (8-Ohdg), Serum Coenzyme Q10 and Lipid Peroxidation in the Formation Attacks of Patients With Multiple Sclerosis
(Suleyman Demirel University, 2017) Cokluk, E.; Milanlıoğlu, A.; Huyut, Z.; Çilingir, V.; Alp, H.H.; Aydın, M.N.; Balahoroğlu, R.
Multiple sclerosis (MS) is a demyelinating disease of the nervous system. Evidence about oxidative stress plays an important role in the pathogenesis of MS is increasing day by day. In our study, we aimed to investigate the effect of oxidative DNA damage and oxidative stress in the pathogenesis of MS disease. Blood samples were obtained from during an attack (Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and 30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of oxidized lipids were detected using flourescence dedector with high pressure liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and patients with MS and then we measured 8-hydroxy-2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum MDA level of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). 8-OHdG/106 dG ratio of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared with group 2 and group 3 (p<0.001). In conclusion, we observed that oxidative DNA damage, lipid and mitochondria oxidative damage were high in blood of patients with MS. It seems that oxidative stress acts a play role the pathogenesis of MS patients as well as induces attacks. © 2017 Suleyman Demirel University. All rights reserved.
Thiol/Disulphide Homeostasis and Oxidative Stress in Patients With Peripheral Facial Paralysis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Demir, C.Y.; Bozan, N.; Kocak, O.F.; Cokluk, E.; Sultanoglu, Y.; Ersoz, M.E.
The main pathophysiological mechanism responsible from the peripheral facial paralysis (PFP) is the ischemia, inflammation and swelling of the facial nerve. The purpose of the present study was to assess the oxidative stress parameters including the thiol/disulphide homeostasis in patients with peripheral facial paralysis. A total of 32 patients with PFP and 32 healthy controls were recruited in this cross-sectional study. Serum samples were compared for thiol/disulphide homeostasis (TDH), total oxidant status (TOS), total antioxidant capacity (TAC), paraoxonase (PON), stimulated paraoxonase (SPON), arylesterase (ARES), ceruloplasmin (CLP), myeloperoxidase (MPO), and catalase (CAT) levels. There were no significant differences between PFP patients and the control group regarding age and gender distribution. Remarkably, TOS (p=0.034), CAT (p<0.001), ARES (p<0.001), native thiol (p<0.001), total thiol (p<0.001), and native thiol/total thiol ratio (p<0.001) were significantly higher in the control group. In contrast, serum ceruloplasmin level (p=0.005) as well as disulphide/native thiol (%) (p=0.001) and disulphide/total thiol (%) (p<0.001) ratios were found to be higher in PFP patients compared to the control group. Thiol/disulphide homeostasis that was suggested as a useful indicator of oxidant/antioxidant imbalance may be a practical marker in diagnosis and follow-up of PFP. Further studies are warranted to determine the effects of nutritional and therapeutic approaches for normalization of oxidative stress in treatment and prevention of PFP. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Tsh Levels in Pregnant Women With Iodine Deficiency Before Spontaneous Abortus
(Editura Acad Romane, 2014) Atmacai, M.; Ozturk, M.; Ozbay, M. F.; Ergenc, E.; Gonullu, E.; Cokluk, E.
Background. Iodine deficiency and/or thyroid autoimmunity are the most common causes of hypothyroidism development among pregnant women. In this study, we aimed to investigate the effect of iodine consumption and thyroid autoimmunity on TSH levels and abortus. The study sample consisted of 104 patients, 79 in abortus and 25 in control groups. TSH, free T4, free T3, anti-TPO, anti-Tg, spot urinary iodine concentrations, and thyroid volumes of the cases were measured by ultrasonography. The spot urine concentration was below 100 mu g/L in 93% of the cases included in the study. The TSH levels of the abortus group cases were significantly higher than those of the controls (p=0.025). The percentage of subclinical hypothyroid cases were significantly higher among the cases evaluated due to abortus compared to the control group (p<0.001). Abortus and control groups did not differ statistically with respect to the presence of autoimmune thyroid diseases (p=0.424). Spot urine iodine concentrations of abortus cases with subclinical hypothyroid were significantly lower than those with TSH levels below the defined range (p=0.001). Spot urine iodine concentrations of the cases with subclinical abortus with negative thyroid autoantibodies were also significantly lower than those with TSH levels below the defined range (p=0.017). TSH levels above 1 mu IU/mL for the first trimester and 2 mu IU/mL for the second trimester may be indicators of iodine nutrition in pregnancy losses.