Browsing by Author "Cokluk, Erdem"

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Association of Cord Blood Ischemia-Modified Albumin Level With Abnormal Foetal Doppler Parameters in Intrauterine Growth-Restricted Foetuses
(Taylor & Francis Ltd, 2021) Andic, Esra; Karaman, Erbil; Kolusari, Ali; Cokluk, Erdem
Objective: To investigate cord blood ischemia-modified albumin (IMA) levels in pregnancies with intrauterine growth restriction (IUGR) and to determine its association with abnormal fetal Doppler findings.Methods: Umbilical cord IMA levels were assessed in 34 pregnant women with IUGR and 32 pregnancies with normal fetal development. Associations of IMA with abnormal umbilical artery Doppler findings, preeclampsia, and oligohydramnios were investigated. IMA was measured using a colorimetric test based on a decrease in cobalt binding.Results: No significant between group differences in maternal age, body mass index, gravida, and parity were identified. The mean gestational age at delivery was earlier in the IUGR group than in the control group (35.73.2 versus 38.4 +/- 1.2, respectively). The mean cord blood IMA values for the IUGR group were significantly increased compared to those in the control group (0.565 +/- 0.22 versus 0.250 +/- 0.12, respectively, p=.001). There was a significant positive correlation between umbilical artery pulsatility index and IMA levels in the IUGR group. Patients with preeclampsia, oligohydramnios, and abnormal nonstress test results in the IUGR group had significantly higher IMA levels. Patients with systolic to diastolic ratios >3 and pulsatility index (PI) above the 95th percentile in the IUGR group had significantly higher cord blood IMA levels (p=.001 and p=.007, respectively).Conclusions: Cord blood IMA values may be a useful marker for perinatal asphyxia. Abnormal Doppler findings are associated with increased IMA levels in complicated pregnancies with IUGR.
Betatrophin Association With Serum Triglyceride Levels in Obstructive Sleep Apnea Patients
(Wolters Kluwer Medknow Publications, 2019) Sertogullarindan, Bunyamin; Komuroglu, Ahmet Ufuk; Ucler, Rifki; Gunbatar, Hulya; Sunnetcioglu, Aysel; Cokluk, Erdem
Background: Obstructive sleep apnea (OSA) is a common sleep problem, in which patients are at increased risk for metabolic and cardiovascular problems, including metabolic syndrome, diabetes mellitus (DM), and dyslipidemia. Betatrophin is a novel protein that regulates fatty acid and triglyceride (TG) metabolism and is related to obesity and metabolic abnormalities, including metabolic syndrome, DM, and dyslipidemia. Although OSA and betatrophin share common abnormalities, their relationship has not been investigated. AIM: The aim of this study is to investigate the relationships among betatrophin, OSA, and the serum lipid profile. METHODS: Ninety consecutive patients with suspected OSA underwent polysomnography (PSG) to confirm OSA. Plasma betatrophin, leptin, adiponectin, and the full lipid profile were analyzed. The patients were categorized as OSA or control based on the apnea-hypopnea index (AHI). RESULTS: About 61% of patients had OSA, and 39% had normal PSG. The levels of betatrophin, leptin, and adiponectin were higher in patients with OSA (256.59 +/- 29.35, 374.20 +/- 37.93, and 17.86 +/- 2.63 mu g/mL, respectively) compared to the controls (141.86 +/- 26.20, 205.53 +/- 14.75, and 7.52 +/- 1.02 mu g/mL, respectively). Betatrophin levels were correlated with the AHI, leptin (r = 0.413, P = 0.002, r = 0.782, P = 0.000). TG levels were significantly higher, and high-density lipoprotein cholesterol (HDL-C) levels were lower, in OSA patients compared to controls (244 +/- 20.33 vs. 138 +/- 14.89, and 37.21 +/- 1.26 vs. 43.78 +/- 1.62, respectively). The TG level was correlated with betatrophin (r = 0.353, P = 0.013). Multiple regression analysis showed that the AHI, leptin, and arousals were independent predictors of betatrophin level (B = 1.70 P = 0.046 95%, B = 0.56 P < 0.005, and B = 1, 2, P = 0.003, respectively). CONCLUSIONS: Our results suggest a complex relationship between OSA, betatrophin, TG, and HDL, as well as other adipokines. Our results require further investigation to assess this complex association and re-evaluate previous related studies.
A Comparison of the Effects of Thymoquinone, Silymarin and N-Acetylcysteine in an Experimental Hepatotoxicity
(Elsevier France-editions Scientifiques Medicales Elsevier, 2018) Demiroren, Kaan; Basunlu, Mehmet Turan; Erten, Remzi; Cokluk, Erdem
This study investigated the effects of thymoquinone, silymarin, and N-acetylcysteine in a rat model with carbon tetrachloride (CCl4)-induced hepatotoxicity. Although numerous similar studies are available, we aimed to compare the efficacy of these agents by considering N-acetylcysteine as a reference compound. A total of 50 male Wistar albino rats were randomly designated as 5 groups: Group I, CCl4; group II, thymoquinone and CCl4; group III, silymarin and CCl4; group IV, N-acetylcysteine and CCl4; group V, control group. CCl4 was administered intraperitoneally at a dose of 1.5 mL/kg (a mixture of CCl4: olive oil, 1:2) twice a week. Thymoquinone was administered at a dose of 10 mg/kg, silymarin was administered at a dose of 100 mg/kg, and N-acetylcysteine was administered at a dose of 100 mg/kg by daily intraperitoneal injection. At the end of four weeks, blood and liver tests were analyzed. The results were evaluated statistically via the one-way ANOVA test. A p-value < 0.05 was considered statistically significant. Thymoquinone, silymarin, and N-acetylcysteine improved the levels of alanine aminotransferase, tumor necrosis factor-alpha, platelet-derived growth factor-BB, and interleukin-6, which were increased by CCl4. Thymoquinone and silymarin showed the positive increase in liver glutathione levels. Thymoquinone, silymarin, and N-acetylcysteine improved blood total oxidant status. In the histological examinations of liver tissue, thymoquinone decreased necrosis, and inflammation. The most positive decrease in the alpha-smooth muscle actin-stained hepatic stellate cell count was only observed with thymoquinone. These findings suggest that thymoquinone, silymarin, and N-acetylcysteine have potential for the treatment of diseases causing liver injury. Among these agents, thymoquinone showed the best results on most of the parameters. Since TQ appears to be at least as effective as SM and NAC in our in-vitro study, we propose that it is time for clinical studies with thymoquinone on hepatotoxicity.
Determining Oxidant and Antioxidant Status in Patients With Genital Warts
(Taylor & Francis Ltd, 2015) Cokluk, Erdem; Sekeroglu, Mehmet Ramazan; Aslan, Mehmet; Balahoroglu, Ragip; Bilgili, Serap Gunes; Huyut, Zubeyir
Objectives: Warts are abnormal skin growths caused by human papilloma virus (HPV) infections within the skin of patients. Genital warts usually appear in the perianal and perigenital regions. Asymptomatic warts may be activated after years and may damage natural immunity. The inflammation that occurs during this process may lead to an imbalance between the prooxidant and the antioxidant systems. The aim of this study was to investigate erythrocyte glutathione peroxidase (GSH-Px) activity, serum paraoxonase enzyme levels, and oxidative stress levels in patients with genital warts. Patients and Methods: In total, 32 patients with genital warts and 35 healthy subjects were included in this study. Erythrocyte GSH-Px activity, serum catalase activity, and paraoxonase enzyme, and malondialdehyde (MDA) levels were determined. Results: Erythrocyte GSH-Px activity, serum MDA levels, and catalase activity were significantly higher in patients with genital warts than in controls (P < 0.01, P < 0.05, and P < 0.05, respectively). However, serum paraoxonase enzyme levels were not significantly different between groups (P > 0.05). Serum triglyceride levels were significantly lower in patients with genital warts than in controls (P < 0.01). However, there were no statistically significant differences between groups with respect to total cholesterol, high-density lipoprotein cholesterol, or low-density lipoprotein cholesterol levels (all P > 0.05). Conclusions: Our data suggest that oxidative stress is increased in genital warts. Increased oxidative stress levels may contribute to the pathogenesis of genital warts, and prolonged HPV infection due to chronic inflammation could also affect oxidative stress.
Effect of Modified Fujita Technique Uvulopalatoplasty on Oxidative Dna Damage Levels in Patients With Obstructive Sleep Apnea Syndrome
(Lippincott Williams & Wilkins, 2015) Bakan, Ebubekir; Fidan, Vural; Hakan, Hamit; Baygutalp, Nurcan Kilic; Cokluk, Erdem
Snoring is a social hindrance problem and it can cause life threatening problems. Because of this it must be taken seriously and must be treated. Although there are many ways for treating this problem, still uvulopalatopharngoplasty (UPPP) which is an accepted classical method maintains its importance. Antioxidant status in patients with snoring have been investigated. All studies investigated the effect of CPAP treatment on the level of antioxidant agents. In this study we have examined the effect of UPPP on the level of antioxidant agents in patients with snoring.
Evaluation of Plasma Agmatine Level and Its Metabolic Pathway in Patients With Bipolar Disorder During Manic Episode and Remission Period
(Taylor & Francis Ltd, 2019) Yilmaz, Emine; Sekeroglu, M. Ramazan; Yilmaz, Ekrem; Cokluk, Erdem
Objectives: Agmatine is a cationic amine resulting from the decarboxylation of l-arginine. Agmatine has neuroprotective, anti-inflammatory, anti-stress, and anti-depressant properties. In this study, plasma agmatine, arginine decarboxylase, and agmatinase levels were measured during manic episode and remission period in patients with bipolar disorder. Methods: Thirty healthy volunteers and 30 patients who meet Bipolar Disorder Manic Episode diagnostic criteria were included in the study. Additionally, the changes in the patient group between manic episode and remission period were examined. We evaluated the relationship between levels of l-arginine and arginine decarboxylase in the agmatine synthesis pathway, and level of agmatinase that degrades agmatine. Results: Levels of agmatine and l-arginine were significantly increased than control group during manic episode (p < .01). All parameters were increased during manic episode compared to remission period (p < .05). Agmatinase was significantly decreased both during manic episode (p < .01) and remission period (p < .05) in comparison to the control group. Arginine decarboxylase levels did not show a significant difference between the groups (p > .05). Conclusions: This study indicate that there may be a relationship between bipolar disorder and agmatine and its metabolic pathway. Nonetheless, we believe more comprehensive studies are needed in order to reveal the role of agmatine in etiology of bipolar disorder.
Investigation of Galectin-3, Lipocalin 2, Retinol Binding Protein (Rbp), Small Dense Low-Density Lipoprotein (Sdldl) in Patients With Hirsutism
(Termedia Publishing House Ltd, 2019) Yavuz, Ibrahim Halil; Ozaydin Yavuz, Goknur; Cokluk, Erdem; Kurtoglu, Zehra; Bilgili, Serap Gunes
Introduction: Hirsutism is defined as excessive terminal hair in androgen-dependent areas in women. Adipose tissue is no longer regarded as a storage site for triglycerides or as a source of free fatty acids but is currently emerging as a key constituent of energy metabolism secreting numerous enzymes, cytokines, growth factors, and hormones. Aim: To evaluate serum levels of galectin-3 (Gal3), lipocalin-2 (LCN2), retinol binding protein (RBP), and small dense low-density lipoprotein (sdLDL) in patients with hirsutism and patients co-presenting with polycystic ovary syndrome (PCOS) and hirsutism. Material and methods: The study included 90 patients that were divided into three groups: (I) patients with idiopathic hirsutism (IH) (n = 30), (II) PCOS patients with hirsutism (H-PCOS) (n = 30), and (III) the control group (n = 30). Informed consent was obtained from each participant. Idiopathic hirsutism was scored using the modified Ferriman-Gallwey (mFG) scoring system and the diagnosis of PCOS was established based on the modified Rotterdam criteria. Human Gal3, LCN2, RBP, and sdLDL levels were analysed using a commercially available double-antigen sandwich enzyme-linked immunosorbent assay. Results: No significant difference was found between the three groups with regard to serum Gal3, LCN2, RBP, and sdLDL levels (p > 0.05). Median values were higher in the H-PCOS group than in the other two groups: body weight (median: 76.5 kg) (p < 0.001), waist circumference (median: 82.5 cm) (p = 0.001). Conclusions: No significant correlation was found between the adipokines that play a role in the aetiology of numerous diseases and some mediators of the lipid metabolism and hirsutism.
The Levels of Nitrite, Nitrate and Lipid Peroxidation in Diabetic Mouse Brain: the Effect of Melatonin and Pentoxifylline
(Taylor & Francis Ltd, 2022) Yalcinkaya, Ahmet S.; Sekeroglu, Mehmet Ramazan; Huyut, Zubeyir; Cokluk, Erdem; Ozbek, Hanefi; Ozturk, Gurkan; Balahoroglu, Ragip
Objective: This study investigated the relationship between diabetes (DM) and nitrite, nitrate and MDA levels and effect of melatonin and pentoxifylline. Methods: Sixty mice were randomly divided into four groups. Control: no action; Diabetes group (DM): after fasting-blood-glucose (FBG) was measured, 150 mg/kg alloxane was applied intraperitoneally three-times every other day; Diabetes + Melatonin (DM + MLT) and Diabetes + Pentoxifylline groups (DM + PTX): following the same procedures with DM, 10 mg/kg melatonin and 50 mg/kg pentoxifylline were administered subcutaneously six days, respectively. Following FBG analysis, brain tissues were taken under the anaesthesia. Nitrite, nitrate and MDA levels were measured. Results: In the all groups with alloxane, FBG were higher than in before application (p < .05). Also, FBG, nitrite, nitrate and MDA levels in the DM + MLT and DM + PTX groups were lower than in the DM (p < .05). Conclusions: Nitrite and nitrate may be related to etiopathogenesis of DM, and pentoxifylline and especially melatonin relatively decrease nitrite, nitrate and lipid peroxidation.
The Relationship of Oxidation Sensitivity of Red Blood Cells and Carbonic Anhydrase Activity in Stored Human Blood: Effect of Certain Phenolic Compounds
(Hindawi Ltd, 2016) Huyut, Zubeyir; Sekeroglu, Mehmet Ramazan; Balahoroglu, Ragip; Karakoyun, Tahsin; Cokluk, Erdem
It has been reported that many modifications occur with the increase of oxidative stress during storage in erythrocytes. In order to delay these negative changes, we evaluated whether the addition of substances likely to protect antioxidant capacity in stored blood would be useful. Therefore, we investigated the effects of resveratrol, tannic acid, and caffeic acid in lipid peroxidation and antioxidant capacity of erythrocytes in stored blood. Donated blood was taken into four CPD containing blood bags. One bag was used as the control, and the others were supplemented with caffeic acid (30 mu g/mL), resveratrol (30 mu g/mL), and tannic acid (15 mu g/mL), respectively. Erythrocyte lipid peroxidation, sensitivity to oxidation, glutathione levels and carbonic anhydrase, glutathione peroxidase, and catalase activities were measured on days 0, 7, 14, 21, and 28. In the control group, erythrocyte malondialdehyde levels and sensitivity to oxidation were increased whereas glutathione, glutathione peroxidase, and catalase levels were decreased (P < 0.05). Resveratrol and caffeic acid prevented malondialdehyde accumulation and preserved glutathione, glutathione peroxidase, and catalase activities in erythrocytes. We demonstrated that resveratrol, caffeic acid, and tannic acid in stored blood could decrease the sensitivity to oxidation of erythrocytes in vitro but did not exhibit such effects on CA activity.
The Susceptibility To Autoxidation of Erythrocytes in Diabetic Mice: Effects of Melatonin and Pentoxifylline
(Wiley, 2017) Sekeroglu, Mehmet Ramazan; Huyut, Zubeyir; Cokluk, Erdem; Ozbek, Hanefi; Alp, Hamit Hakan
Oxidative stress had a great importance in development of complications in diabetes. We investigated effects of melatonin and pentoxifylline in diabetic mice. Swiss albino mice (n=40) were divided into four groups: alloxan-induced diabetes mellitus (DM), alloxan-induced diabetes with melatonin supplementation (DM+MLT), alloxan-induced diabetes with pentoxifylline supplementation (DM+PTX), and control. Glutathione-peroxidase (GSH-Px) activity, malondialdehyde (MDA) and reduced glutathione (GSH) levels, and susceptibility to oxidation of erythrocytes were measured. MDA levels were higher than control in the DM and DM+MLT. The DM had more MDA level than the DM+MLT and DM+PTX (P<0.001). After in vitro oxidation, MDA levels of all groups were found higher than the control. However, they were significantly lower than the DM in DM+PTX and DM+MLT (P<0.001). Although GSH levels of the DM and DM+PTX were less than the control, GSH-Px activity of the DM was lower than the control and DM+PTX (P<0.05). We suggest that there is increased oxidative stress and compromised antioxidant status of erythrocytes in diabetes; however, it can be effectively prevented by melatonin or pentoxifylline supplementation.
Vitamin B12, Folate Levels and Somatoform Dissociation in Conversion Disorder
(Pakistan Medical Assoc, 2020) Ozdemir, Pinar Guzel; Gur, Tugba; Cokluk, Erdem; Isik, Mesut; Tapan, Suheda
Objective: To evaluate the association of folate and vitamin B12 levels in patients with conversion disorder, and to illuminate the aetiology of conversion disorder by examining depression and somatoform dissociation. Method: The case-control study was conducted from March 2014 to May 2015 at the Medical Centre of Yuzuncu Yil University, Van, Turkey, and comprised patients diagnosed with conversion disorder and healthy controls. Blood samples were taken from both groups for vitamin B12 and folate levels. Data was collected using the Beck Depression Inventory and Somatoform Dissociation Scale. Data was analysed using SPSS 18. Results: Of the 100 subjects, 55(55%) were cases with a mean age of 27.05 +/- 9.04 years and 45(45%) were controls with a mean age of 26.56 +/- 5.96 years. The mean level of B12 was 283.93 +/- 122.96 in cases and 324.62 +/- 128.82 in controls (p=0.05). The mean level of folic acid was 5.47 +/- 1.84 in cases and 6.07 +/- 2.26 in controls (p>0.05). Conclusions: Physicians need to be vigilant about vitamin B12 levels in patients with conversion symptoms.
Within-Subject and Between-Subject Biological Variation of First Morning Void Urine Amino Acids in 12 Healthy Subjects
(Walter de Gruyter Gmbh, 2020) Alp, Hamit Hakan; Akbay, Halil Ibrahim; Cokluk, Erdem; Huyut, Zubeyir; Keskin, Siddik; Sekeroglu, Mehmet Ramazan
Background: Urine amino acid analysis is used for the assessment of various diseases. The aim of this study was to estimate the valid biological variation (BV) components (within- and between-subjects) required for the safe clinical application of free urine amino acids. Methods: First morning void urine samples were taken from 12 healthy subjects (five females, seven males) once a week for 10 consecutive weeks, and amino acid analysis was performed using an Agilent 6470 triple quadrupole tandem mass spectrometer instrument. The obtained data were subjected to normality, outlier and variance homogeneity analyses prior to coefficient of variation (CV) analysis. Within- and between-subject BV values (CVI and CVG) of 39 amino acids were determined for all subjects. In addition, the index of individuality (II), reference change value (RCV), imprecision, bias and total error were estimated using BV data obtained from our study. Results: The CVI values ranged from 8.9 (histidine) to 36.8% (trans-4-hydroxyprolin), while the CVG values ranged from 25.0 (1-methyl-L-histidine) to 63.3% (phenylalanine). The II value of most amino acids was less than 0.6 and ranged between 0.21 and 0.88. The imprecision, bias and total error ranged between 4.45 and 16.6, between 7.69 and 16.6, and between 18.4 and 43.2, respectively. Conclusions: This study, designed according to a rigorous protocol, has the feature of being the first to give information about BV data of urine amino acids. We believe that the reference intervals have a limitation in the evaluation of consecutive results from an individual, so the use of RCV would be more appropriate.