Browsing by Author "Cokmus, C"

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Characterization of Staphylococcus Species by Sds-Page of Whole-Cell and Extracellular Proteins
(Maik Nauka/interperiodica, 2003) Berber, I; Cokmus, C; Atalan, E
In this study, a total of fifteen staphylococcal strains belonging to different species were characterized by whole-cell and extracellular protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results are presented as dendrograms after quantitative analysis of the band patterns with a computer program. Visual inspection of protein bands and cluster analysis of protein patterns of to be used 15 strains, representing 10 Staphylococcus species, showed that whole-cell and extracellular protein profiles differed in several protein bands in Staphylacoccus aureus, S. epidermidis, S. simulans, and other species of Staphylococcus; however, the differences were insufficient for reliable differentiation of Staphylococcus species by the SDS-PAGE method.
The Influence of Pesticides on the Spore Viability, Toxin Stability and Larvicidal Activity of Bacillus Sphaericus 2362 Strain
(Parlar Scientific Publications (p S P), 2004) Berber, I; Atalan, E; Cokmus, C
In this study, 12 different commercial pesticides, including 5 fungicides, 5 herbicides, 1 aphicide and 1 acaricide, were investigated for their effects on spore viability, toxin stability and larvicidal activity of a mosquito pathogenic B. sphaericus 2362 strain. Minimal inhibitory concentrations (MICs) of each pesticide were determined after 48 h incubation of B. sphaericus 2362 strain. According to MIC results, all pesticides showed various inhibitory effects on the growth of the test bacterium. Particularly, the pesticides which contain TCA, hymexazol, copper sulphate, hexythiazox, 2,4-D Amine, copper inhibited spore germination significantly and bacterial growth even at the lowest tested concentrations. Our results indicated that various commercial pesticides in different concentrations reduced the spore viability significantly and heat-resistant spore numbers of B. sphaericus 2362 strain. In general, the pesticides decreased the spore numbers 10(4)-10(5)-fold compared to initial ones. Furthermore, it was observed that the larvicidal activity was completely lost when it was grown at the concentration of MIC values or higher. Indeed, SDS-PAGE analysis confirmed that the binary toxin proteins, 41.9 kDa and 51.4 kDa, were lost at the MIC values and higher concentrations although parasporal crystals did not disappear in the much lower pesticide concentration.