Browsing by Author "Comba, Arzu"

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Arpa Çiminin Diyabetli Ratlarda Antioksidan Kapasitesine ve Dna Hasarına Etkisi
(2023) Başbuğan, Yıldıray; Comba, Bahat; Aksu, Devrim Sarıpınar; Comba, Arzu; Mis, Leyla
Amaç: Dünya çapında fitoterapi yöntemleri büyük önem kazanmakta ve bu alandaki çalışmalar her geçen gün önemini artırmaktadır. Bu çalışmada deneysel olarak diyabet oluşturulan ratlarda toplam antioksidan, oksidan durum, oksidatif DNA hasarı, glikoz ve hemoglobin A1c düzeyleri ve arpa çimi suyunun bu parametreler üzerine etkisinin incelenmesi amaçlandı. Gereç ve yöntem: Çalışma için 4 grup oluşturuldu ve her grupta 250-350 gr ağırlığında 6 erkek rat kullanıldı. Kontrol grubu; serum fizyolojik periton içi yol ile enjekte edildi, Diyabet grubu; diyabet, streptozotosin kullanılarak oluşturuldu, Arpa çimi grubu; 4 hafta oral olarak arpa çimi suyu (3 ml/sıçan/gün) verildi, Diyabet+Arpa çimi grubu; streptozotosin ile diyabet oluşturuldu ve 4 hafta boyunca arpa çimi suyu oral olarak verildi. Bulgular: Arpa çimi suyu, streptozotosin kaynaklı diyabette kan şekeri, glikoz, HemoglobinA1c, toplam oksidatif durum ve oksidatif stres gösterge değerlerini düşürdüğü, toplam antioksidan değerini ve canlı ağırlıkları arttırdığı belirlendi. Ayrıca arpa çimi suyu ilavesinin bu parametreler üzerinde belirgin koruyucu etki ve iyileşme sağladığı tespit edildi. Sonuç: Bu bulgulardan yola çıkarak arpa çimi suyunun şeker hastalığında anti-diyabetik, anti-oksidan etki gösterdiği ve kilo kaybını önlediğini söyleyebiliriz.
Assessment of the Wheatgrass Effects on Antioxidant Capacity and Dna Damage in Rats With Experimental Renal Failure
(Parlar Scientific Publications (p S P), 2019) Mis, Leyla; Comba, Bahat; Basbugan, Yildiray; Yasar, Semih; Comba, Arzu
In this study, it was aimed to evaluate the effect of wheatgrass on antioxidant capacity and DNA damage in rats with renal failure. In the study, 200-300 g 24 healthy Wistar-Albino male rats obtained from YYU Experimental Animal Unit were used. The rats were randomly divided into 4 groups and each group was formed with 6 rats. Groups were allocated as; 1. Control Group, 2. Renal Failure Group (Genta Group) 3. Renal Failure + Wheatgrass Group (Genta +Wheatgrass) 4. Wheatgrass Group. Gentamicin sulfate was given intraperitoneal at a dose of 50 mg/kg for 1 week and once a day to the groups with renal failure (Groups 2 and 3). Wheat grass extract (10 ml/kg) was added to the drinking water of the wheatgrass group rats (3rd and 4th groups) once a day. This practice lasted for 4 weeks. Total oxidant, antioxidant quantities, DNA damage were evaluated at the end of 4 weeks. The result that wheatgrass could be useful in renal failure was reached.
The Effect of Barley Grass on Antioxidant Capacity and Dna Damage in Rat With Renal Failure
(Parlar Scientific Publications (p S P), 2017) Comba, Bahat; Mis, Leyla; Uslu, Sema; Comba, Arzu
This study was aimed to search the effect of barley grass (BG) on the Total Antioxidant (TAS)-Oxidant Status (TOS) and DNA damage (8OHdG) in rat with renal failure. The rats which are used in the study were randomly divided into 4 groups that each of has 8 rats: Control (C) group; injected i.p. with physiological saline once a day for 7 days, GM group; Gentamicin (80 mg/kg/day) was injected i.p. for 7 days, BG group; was given oral BG (250 mg/kg/day) for 4 weeks, GM+BG group was injected gentamicin (80 mg/kg/day) with i.p. for 7 days and BG (250 mg/kg/day) was given by oral for 4 weeks. After from the process of experiment for 4 weeks, blood sample and kidney tissue were taken. The analyses of urea and creatinine were done by autoanalyser; TAS, TOS levels by colorimetric kits; 8OHdG level by ELISA kits in serum. The kidney tissues were examined histologically. In the GM+BG group was statistically decreased levels of urea, creatinine, TOS (p<0.05) and OSI (p<0.01) compared to GM group. In the group of BG was determined the levels of TAS p<0.05 statistically increased other groups. The statistical significance was not found in the level of serum 8OHdG differences between the groups. BG decreased widespread damage to the renal glomerulus and proximal tubulus. As a result, adverse effects of high dose gentamicin application such as kidney damage can be reduced when used barley grass.
The Effect of Chemical and Surgical Castration on the Live Weight Gain and Some Hormones of Male Norduz Sheep
(Zoological Soc Pakistan, 2017) Karakus, Kadir; Comba, Bahat; Tas, Abuzer; Sancak, Tunahan; Comba, Arzu; Aksu, Devrim Saripinar; Tariq, Mohammad Masood
The aim of this study was to investigate the effects of surgical and chemical castration on body weight and some hormones. In the present study, 2.5-3-month-old weaned male Norduz sheep were used. The sheep were obtained in the year 2015 from Livestock Application and Research Directorate of Yuzuncu Yil University. The sheep were randomly allocated into three groups each of which contained 10 sheep. The groups were formed as; Group 1 The Control Group (No castration), Group 2 (Surgical castration group) and Group 3 (Chemical castration group), respectively. Blood sample was taken from vena jugularis of the animals before the application and every two months for a total of 4 times for 6 months and hormone analyses were performed. The order of statistical significance for TSH (thyroid stimulating hormone) were found as Surgical > Control > Formol (P<0.05). The order of statistical significance for growth hormone was Formol = Surgery > Control (P<0.05). In August month, the application factor was found to be significant only in testosterone (P<0.05). No significant difference between the control and surgical groups in testosterone was noted. In this study, although the application of different castration methods in 4-month-old male Norduz sheep resulted in changes in the blood hormone levels, it was determined that these changes did not affect body weight. In conclusion, the evaluation of the process in castrated six-month-old sheep revealed an increase in the body weight which was similar to that in the control group.
Effect of Chronic Exposure To Sodium Fluoride and 7,12-Dimethylbenz[a]anthracene on Some Blood Parameters and Hepatic, Renal, and Cardiac Histopathology in Rats
(int Soc Fluoride Research, 2018) Yildirim, Serkan; Ekin, Suat; Huyut, Zubeyir; Oto, Gokhan; Comba, Arzu; Uyar, Hasan; Cinar, D. Ali
This study aimed to investigate the effects of both sodium fluoride (NaF) and 7,12-dimethylbenz[a]anthracene (DMBA), both separately and in combination, on some blood parameters and hepatic, renal, and cardiac histopathology in rats. Forty male Wistar albino rats, weighing 250-300 g, were randomly divided into one control and three experimental groups (i) a NaF group who received 15 ppm of NaF in their drinking water for 90 days, (ii) a DMBA group who received 10 mg DMBA/kg body weight/po/ weekly for 90 days, and (iii) a NaF+DMBA group who received 15 ppm NaF in their drinking water plus 10 mg DMBA/kg bw/po/weekly for 90 days. The animals in the groups were sacrificed at the end of the 90 days. The AST, ALT, LDH, CK, creatinine, troponin I, and MDA levels increased in the NaF, DMBA, and NaF+DMBA groups compared to the control group, while the WBC, K, Na, Cl, urea, SOD, GSH-Px, CAT, and GSH values showed a statistically significant decrease (p<0.05). In addition, the CK-MB significantly increased in the DMBA and NaF+DMBA groups compared to the control group (p<0.05). The histological structure of the liver, kidney, and heart tissues in the control group was normal. In the NaF and DMBA groups, degenerative and necrotic changes were detected. In the NaF+DMBA group: (i) the liver exhibited hydropic degeneration and coagulation necrosis in hepatocytes, severe dilation in the sinusoids, congestion in the central and portal regions, and mononuclear cell infiltration in the portal region; (ii) the kidneys displayed congestion in the glomerulus and interstitial vessels, interstitial nephritis, diffuse hydropic degeneration, and coagulation necrosis in the tubule epithelium; (iii) the heart showed myocardial hyperemia, severe mononuclear cell infiltration in interstitial tissue, hyaline degeneration, and Zenker's necrosis in myocardium As a result of these blood and oxidative stress parameters and histopathological findings, it was determined that NaF, DMBA, and NaF+DMBA induce toxicity in the liver, kidney, and heart tissues and thus play an important role in the physiopathology of toxicity.
Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene
(Kafkas Univ, veteriner Fakultesi dergisi, 2016) Comba, Bahat; Oto, Gokhan; Mis, Leyla; Ozdemir, Hulya; Comba, Arzu
In this study was investigated effects of borax (BX) on inflammation markers, haematolojical parameters and total oxidant (TOS)-antioxidant status (TAS) in rats applied 3-methylcholanthrene (3-MC). In this research a total of 24 Wistar Albino rats were used. They were divided into 4 groups each containing 6 rats. 1st group was separated as a control group. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose to the 2nd group with i.p. way. BX was given to 3rd group 300mg/L/day dose with drinking water during 150 days. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose with i.p. way and BX were given with drinking water during 150 days to 4th group. At the end of the study blood analysis, tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) levels in 3-MC group; TOS and oxidative stress index (OSI), platelet (PLT) levels in 3-MC and 3-MC+BX groups showed significantly increases when compared to other groups. It was determined that lymphocytes % (LY%) of ever 3 groups were significantly higher; however, neutrophil % (NEU%) were significantly fewer according to control group. Haemoglobin (HGB) and hematocrit (HCT) values of 3-MC+BX groups showed significantly decrease according to other groups (P <= 0.05). Mean corpusculer volume (MCV) in 3-MC and 3-MC+BX groups showed significantly decrease when compared to other groups (P <= 0.05). As a result, in case of exposure to 3-MC, long-term use of BX with oral ways may not decrease oxidative stress, may changes haematolojical parameters such as, WBC, LY%, NEU%, PLT, HGB, HCT, MCV. However, these changes remain within physiological limits. Even so, in the use of BX should be considered use of iron. Furthermore, BX with the abovementioned dosage may be used to reduce the levels of TNF-alpha, IL-1 beta, IL-6 being inflammation and cancer markers.
Effects of Boric Acid on Proinflammation Cytokines, Total Oxidative-Antioxidative Status and Hematological Parameters in Rats Applied Benzo(A)pyrene
(Parlar Scientific Publications (p S P), 2020) Comba, Arzu; Oto, Gokhan; Comba, Bahat; Ozdemir, Hulya; Keskin, Siddik; Akveran, Gonul Arslan
The aim of this study was to evaluate effects of boric acid (BA) on tumor necrosis factor-alfa (TNF-alpha), interleukin 1 beta (IL-beta), interleukin-6 (IL-6), total oxidative (TOS)-total anti oxidative status (TAS) and leucocyte (WBC), erythrocyte (RBC), thrombocyte (PLT) and their other parameters in rats applied benzo(a)pyrene (B(a)P). In this study, rats were divided into 4 groups and each group were containing 6 rats. 1st group was separated as control group. 100 mg/kg total dosage of B(a)P was applied to the 2nd group 2 times a week total in 4 equal dosage with i.p. way. Only BA 300 mg/L was applied to 3rd group in drinking water every day during 150 days. B(a)P was applied twice a week first 2 weeks, 25 mg/kg dose with i.p. way and BA were given with drinking water during 150 days to 4th group. In the end of study was determined TNF-alpha and IL-1 beta levels in B(a)P group, increases compared with other groups. there was a statistically significant increase in levels of WBC and LY in group B(a)P+BA compared to other groups (P <= 0.05). % LY level in other groups was higher; its % NEU level was lower according to control group. RBC and PLT values in BA group were higher, than the other groups. HGB was higher in group BA than group B(a)P and BA+ B(a)P; HCT was higher in group BA than group B(a)P , significantly (P <= 0.05) As a result, neutropenia and lenfositos develop in case exposure of B(a)P and BA; furthermore, combination of B(a)P and BA increases WBC. BA used alone having considerable effect on excitation of eritopoezis and thrombocytosis. BA has protective effects for organisms together with decrease in the TNF-alpha and IL-1 beta levels caused by BA cure as solution of B(a)P exposition.
Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs With White Muscle Disease
(Agricultural Research Communication Centre, 2021) Irak, Kivanc; Mert, Handan; Mert, Nihat; Aysin, Nesrullah; Sogutlu, Inci Dogan; Comba, Arzu
Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups.
Experimental Study of In-Vitro Bioanalysis and In-Vivo Living Tissue Biocompatibility of Mg-Zn Alloys
(Springer Heidelberg, 2023) Comba, Bahat; Cicek, Bunyamin; Comba, Arzu; Sancak, Tunahan; Akveran, Gonul Arslan; Koc, Erkan; Afshar, Milad Torkamanian
In this study in-vitro bioanalysis and in-vivo living tissue biocompatibility were evaluated. Pure Mg and Mg + x Zn (x = 1 and 3 wt%) alloys have been used in the study. In vitro immersion and potentiodynamic corrosion were applied to these alloys in Hank's and artificial seawater solution. The materials were kept in living tissue (gluteus-superficial) for 7 weeks in-vivo (albino-rat). After 4 and 7 week, the control and experimental groups' urine parameters, blood mineral substance levels, and radiographic image controls were evaluated. It was observed that the bio-dissolution level decreased as the Zn-ratio increased. As a result, successful results were obtained in the biocompatible and biodegradable material class of Zn-doped Mg alloys used in the study. The dissolution amount of 1.03 mm/y (for Mg + 3Zn alloy) in Hank's solution was calculated. The % vitality was determined at the level of 117%.
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimetilbenz(A)antrasen (Dmba) Affect Erythrocyte Fragility and Parameters in Rats
(Wiley, 2017) Comba, Bahat; Oto, Gokhan; Arihan, Okan; Comba, Arzu; Uyar, Hasan
Investigation of In-Vitro Biocompatibility and In-Vivo Biodegradability of Am Series Mg Alloys
(Taylor & Francis Ltd, 2022) Comba, Arzu; Cicek, Bunyamin; Comba, Bahat; Sancak, Tunahan; Akveran, Gonul Arslan; Sun, Yavuz; Afshar, Milad Torkamanian
In this study, in vitro/vivo biocompatibility experiments of Mg-Al-Mn alloy were carried out, and AM50 and AM60 alloys were used in the experiments. Immersion and potentiodynamic corrosion have been applied to these alloys in vitro. For biocompatibility, cytotoxicity test was performed. A total of 40 rats were used for the in-vivo experiment. AM series alloys were surgically placed in the femoral condyle as mini screws and monitored radiographically. AM alloys were removed together with the femur at the end of 7 weeks and further examined. After in-vivo application, the biodegradability level was successfully concluded with blood mineral analysis and urinalysis. In this study, the bioprocess of AM series Mg alloys with Al and Mn additions has been completed. As a result, the AM alloys used can be classified as biocompatible and biodegradable materials.
Leptin Levels and Lipids Profile Determination in Different Sheep Breeds
(Univ Agriculture, Fac veterinary Science, 2016) Comba, Arzu; Mert, Handan; Comba, Bahat
Leptin hormone is produced by fat cells and is believed to coordinate the control of body weight. This study was conducted to determine the levels of leptin, triglyceride, cholesterol, VLDL, LDL, HDL and glucose in serum apart from the body weight of animals, and to find out the correlations between them. Four breeds of sheep (Karakul; semi-fat tailed breed, Morkaraman and Norduz; fat tailed breeds, Tahirova; thin tailed breed) were used as research material under the same feeding conditions. Fifteen sheep of each group -for a total of 60- were selected as research material. ELISA tests were used to determine serum leptin levels; auto analyzer was used for the estimation of triglyceride, cholesterol, VLDL, LDL, HDL and glucose levels. Body weights were measured by a weighing scale. Leptin levels, along with triglyceride, cholesterol, VLDL, LDL, HDL levels and body weight, were higher in Tahirova sheep breed, and the difference was found statistically significant (P<0.05). There was no statistically significant difference between the HDL levels of sheep breeds. Glucose levels were high in Karakul breed and statistically significant difference was determined between Karakul, Norduz and Tahirova breeds (P<0.05). In conclusion, thin tailed sheep breeds were found to have a high leptin level, which shows that leptin levels are independent of the tail fat ratios. Leptin has been studied in detail in human medicine, but researches on animals were scarce. Apparently, further studies are needed to elucidate the factors of production of leptin in animals. (C) 2015 PVJ. All rights reserved
Leptin Levels and Lipit Profile Determination in Different Sheep Breeds
(2014) Comba, Arzu; Mert, Handan
Leptin, vücut ağırlığı kontrolünü koordine eden ve adipositler tarafından üretilen bir hormondur. Bu çalışma, Yüzüncü Yıl Üniversitesi Araştırma ve Uygulama Çiftliğinde yetiştirilen farklı koyun ırkları arasındaki serum leptin, trigliserit, kolesterol, VLDL, LDL, HDL, glukoz düzeyleri ile canlı ağırlıkların belirlenmesi ve aralarındaki korelasyonların saptanması amacıyla yapıldı. Aynı bakım besleme şartlarında sağlıklı 4 farklı koyun ırkı (Karagül, Morkaraman, Norduz, Tahirova) kullanıldı. Her bir koyun ırkından 15'er adet olmak üzere toplam 60 koyun çalışmaya dahil edildi. Serum leptin seviyeleri, koyun leptin ELISA kiti ile, trigliserit, kolesterol, VLDL, LDL, HDL ve glukoz düzeyleri otoanalizör ile canlı ağırlıkları da tartım yapılarak belirlendi. Koyunlardaki serum leptin, trigliserit, kolesterol, VLDL, LDL, HDL, glukoz seviyelerinin ve canlı ağırlıklarının ortalamaları sırası ile, Karagül ırkında, 12.08±0.82 ng/ml, 36.10±7.02 mg/dl, 70.40±7.84 mg/dl, 7.00±1.46 mg/dl, 17.60±4.01 mg/dl, 45.80±6.91 mg/dl, 57.80±11.53 mg/dl, 37.28±1.12 kg; Morkaraman ırkında, 6.50±3.35 ng/ml, 56.86±31.79 mg/dl, 120.73±48.75 mg/dl, 13.93±10.05 mg/dl, 25.87±6.22 mg/dl, 49.20±9.58 mg/dl, 50.40±10.35 mg/dl, 47.90±3.32 kg; Norduz ırkında, 5.04±2.61 ng/ml, 43.13±24.33 mg/dl, 83.53±16.40 mg/dl, 7.87±3.35 mg/dl, 18.16±3.84 mg/dl, 49.71±7.12 mg/dl, 46.00±9.05 mg/dl, 46.37±2.12 kg; Tahirova ırkında ise, 16.68±6.78 ng/ml, 179.08±113.08 mg/dl, 187.20±44.30 mg/dl, 35.80±22.66 mg/dl, 107.00±30.96 mg/dl, 44.40±11.36 mg/dl, 46.53±11.46 mg/dl, 55.14±2.46 kg olarak belirlendi. Serum leptin, trigliserit, kolesterol, VLDL, LDL seviyeleri ve canlı ağırlıkları Tahirova koyun ırkında en yüksek düzeyde ve istatistiksel önemde bulundu (p<0.001). Serum HDL miktarlarında istatistiksel fark saptanamadı. Glukoz düzeyi en yüksek Karagül ırkındaki koyunlarda bulundu ve Karagül ırkı ile Norduz ve Tahirova ırkı arasında p<0.05 düzeyinde önem tespit edildi. Sonuç olarak, ince kuyruklu koyunlarda leptin düzeyinin yüksek çıkması leptin üretiminin kuyruk yağ oranına bağlı olmadığını göstermektedir. İnsanlarda detaylı olarak çalışılmış ancak evcil hayvanlarda leptin konusunda yeterli çalışma bulunmadığından mekanistiksel olarak leptin üretiminde etkili ve düzenleyici faktörlerin açıklanabilmesi için daha fazla araştırmaya ihtiyaç vardır.
Retinol, Α-Tocopherol and Vitamin D3 in White Muscle Disease
(Polish Soc veterinary Sciences Editorial office, 2018) Mert, Handan; Yildirim, Serkan; Yoruk, Ibrahim Hakki; Irak, Kivanc; Comba, Bahat; Mert, Nihat; Comba, Arzu
Vitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fatsoluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions - like eyesight - and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White Muscle Disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, alpha-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum alpha-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p < 0.001) and alpha-tocopherol (p < 0.001) level differences were statistically relevant. Macroscopically, Zenker's necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker's necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of alpha-tocopherol and retinol (p < 0.001) compared to the sick lambs.
Sheep in Turkey From Wild Types To Domesticated Forms
(2015) Tas, Abuzer; Karakus, Kadir; Comba, Bahat; Koyun, Hasan; Comba, Arzu; Çınaroğlu, Selim
Ülkemiz Ortadoğu ve Avrupa' da yaban hayatı çeşitliliği ile en zengin potansiyele sahip olup, pek çok hayvan türü için gen kaynağı merkezi konumundadır. İnsanının en önemli faktör olarak görüldüğü küresel ısınma, doğal yaşam alanlarının tahrip edilmesi, avlanma nedenleri ile hızla tür, ırk ve gen kaynakları, yani biyoçeşitlilik azalmaktadır. Günümüz evcil koyunların kökenini olumsuz çevre şartlarına dayanıklı ve üreme yeteneği yüksek olarak bilinen Muflon, Arkar ve Argali yabani koyunlar oluşturmaktadır. Ekonomik öneme sahip birçok özelliğinde dolayı koyun, yüzyıllar boyunca evciltme ve buna paralel olarak ıslah çalışmalarına konu olan ilk türlerdendir. Bu süreç içerisinde yabani formda tanımlanan doğal çevreye adaptasyon, türe özgü davranış biçimleri, hayvan refahı ve haklarına ilişkin birçok özelliğin de olumsuz etkilendiğinin söylenmesi yanlış olmayacaktır. Gelecek nesiller için koyun türünün var olması, her şeyden önce olumsuz çevre koşullarına karşın yaşayabilmesi, üremesi için türe ait özelliklerini kaybettirmeden korunması, moleküler düzeyde genomik çalışmaların yapılması ve genotip - çevre ilişkilerinin dikkatle incelenmesi önemlidir
Sıçanlarda Acth Uygulamasının Böbrek Fonksiyon Testleri, Elektrolitler ve Hematolojik Parametreler Üzerine Etkileri
(2016) Çınar, Ali; Comba, Bahat; Comba, Arzu; Gencer, Yıldırım Gökhan
Bu çalışmada, sıçanlarda ACTH uygulamasının böbrek fonksiyon testlerine, elektrolitlere ve hematolojik parametreler üzerine etkilerini araştırmak amaçlandı. Araştırmada kontrol ve stres olmak üzere iki grup oluşturuldu. Her grupta 6'şar adet yaklaşık 13 haftalık, 200-250 g ağırlığında, Sprague Dawley ırkı erkek sıçan kullanıldı. Kontrol grubuna 0.5 ml serum izotonik/birey, stres grubuna ise 4 mg/kg dozunda ACTH intraperitoneal (i.p.) olarak bir kez uygulandı. Bu uygulamadan 3 saat sonra kan örnekleri alındı. Serumda glukoz, amilaz, kreatinin, kreatin kinaz (CK), kreatin kinaz MB (CK-MB), üre, ürik asit, kan üre azotu (BUN), laktat dehidrojenaz (LDH), kalsiyum (Ca), fosfor (P), magnezyum (Mg), sodyum (Na), klor (Cl), potasyum (K) analizleri, tam kanda ise total lökosit (WBC), eritrosit (RBC), hemoglobin (HGB), hematokrit (HCT), trombosit (PLT) ölçümleri yapıldı. Stres grubunda glukoz, WBC, % monosit ve % granülosit düzeyleri P<=0.001 önemde, CK, CK-MB, üre, BUN değerleri P<=0.05 önemde kontrollere göre yüksek bulunurken; % lenfosit (P<=0.001), Na, Cl, ve K (P<=0.05) seviyeleri ise düşük bulundu. Bu sonuçlar, ACTH ile oluşturulan stresin böbrek fonksiyon testleri, elektrolit dengesi ve hematolojik parametreler üzerine etkilerinin dikkate değer olduğunu gösterdi.
Some Biochemical and Haematological Parameters in the Second Trimester of Pregnancy
(2008) Comba, Arzu; Mert, Handan
Van İlinin kırsal kesiminde yaşayan ve gebeliği süresince herhangi bir ilaç (demir, folik asit, vitamin B12) ilavesi almayan, gebeliğinin ikinci trimesterindeki 60 gebe ile aynı yörede yaşayan ve gebe olmayan sağlıklı 30 kadında folik asit, vitamin B12, demir, demir bağlama kapasitesi, transferrin, ferritin, hemoglobin ve hematokrit düzeyleri ile beslenme durumları araştırıldı. Gebe ve kontrol grubunun folik asit ve ferritin ölçümü immunoassay yöntemi ile vitamin B12 analizi competitive chemiluminescent enzyme immunoassay yöntemi ile demir, demir bağlama kapasitesi ve transferrin ölçümleri immunoturbidimetrik yöntemi ile serum da yapılırken; hemoglobin ve hematokrit ölçümleri tam kanda empedans yöntemi ile yapıldı. Kontrol grubunun % 60'ı, gebelerin ise % 58'inin yetersiz beslendiği saptandı. Gebelerde folik asit, vitamin B12, demir, ferritin, hemoglobin ve hematokrit düzeyleri kontrollere göre istatistiksel olarak p < 0.001 önemle düşük bulunurken, demir bağlama kapasitesi ve transferrin p < 0.001 önemle yüksek bulundu. Kırsal ve sosyo-ekonomik olarak düşük şartlarda yaşayan gebe kadınların temelde beslenmeye bağlı olarak hemoglobin düzeylerinde saptanan nispi düşüklük muhtemel bir anemiye işarettir. Sonuç olarak, gebelikte aneminin tedavisi zaman aldığından, maternal ve fetal defektler oluşmasını önlemek için gebelik öncesi anemi teşhisinde kullanılan kritik parametreler ölçülüp, kadınlar anemi yönünden değerlendirmeli, mineral ve vitamin takviyeleri ile sorun engellenmelidir.