Browsing by Author "Comba, B."

Now showing 1 - 8 of 8

Effect of Royal Jelly on Experimental Colitis Induced by Acetic Acid and Alteration of Mast Cell Distribution in the Colon of Rats
(Pagepress Publ, 2010) Karaca, T.; Bayiroglu, F.; Yoruk, M.; Kaya, M. S.; Uslu, S.; Comba, B.; Mis, L.
This study investigated the effects of royal jelly (RI) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg(-1) body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg(-1) body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory's triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RI was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RI treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RI could be used to treat colitis.
Effects of Acth Application on Kidney Function Tests, the Electrolytes and Hematological Parameters in Rats
(Chartered Inst. of Building Services Engineers, 2016) Comba, B.; Çinar, A.; Comba, A.; Gencer, Y.G.
This study was conducted to investigate the effects of ACTH application on kidney function tests, electrolytes, and hematological parameters in rats. In the research two groups were formed, including control and stress. Each group consisted of 6 male Sprague Dawley rats which were about 13 weeks old and 200-250 g in weight. Serum isotonic was administered at a dose of 0.5 ml/rat to control group, and ACTH was applied at a dose of 4 mg/kg to stress group at once intraperitoneally. Blood samples were taken 3 hours after the application. Concentrations of glucose, amylase, creatine, creatine kinase (CK), creatine kinase MB (CK-MB), urea, uric acid, lactate dehydrogenase (LDH), calcium (Ca), phosphorus (P), magnesium (Mg) sodium (Na), chlorine (Cl) and potas-sium (K) in serum; the numbers of total leukocytes (WBC), platelet (PLT) and erythrocytes (RBC) and the values of hemoglobin (HGB) and hematocrit (HCT), on whole blood were performed. The level of glucose, WBC, monocyte %, granulocyte %, p≤0.001 values of stress group were significantly higher than those of control group (p≤0.001) while the values of CK, CK-MB, urea, BUN were significantly higher (p≤0.05) while lymphocytes % (p≤0.001) and the levels of Na, Cl, and K (p≤0.05) were significantly lower in stress group compared to control. These results indicated that stress induced by ACTH had noticeable impact on the renal function test, electrolyte balance and hematological parameters. © 2016, Chartered Inst. of Building Services Engineers. All rights reserved.
Effects of Sildenafil Citrate on the Hematological Parameters in the Early Phase of Wound Healing in Diabetic Rats
(Academic Journals inc, 2011) Tas, A.; Karasu, A.; Comba, B.; Aksu, D. S.; Duz, E.; Tanritanir, P.
In this study Investigation of effects of sildenafil citrate on some of the hematological parameters in the early phase of wound healing in diabetic rats was aimed in this study. Total 30 rats were used in the study. Rats were divided into three groups equal in number (control-non diabetic, diabetic and diabetic+Sc). While no treatment was given to the control-non diabetic and diabetic groups, sildenafil citrate was given to the diabetic+Sc group intraperitoneally with a dosage of 0.7 mg/kg/day dosage for 3 days after creating wounds: under ketamine anesthesia, a circular, full thickness, standard wound was created on the back of each rat by using a 0.8 cm sterile punch. Cardiac blood was drawn in tubes containing EDTA from each group before the creation of wounds and on days 1, 2 and 3 following the creation of wounds. Differential leukocyte counts were determined in blood samples containing anticoagulant. For this purpose, blood smears were prepared from blood samples and stained with Giemsa method. It was found that, while neutrophil counts increased in all the groups during the wound-healing periods with the increasing number of days, lymphocyte counts reduced and in control and Sc+diabetic groups, monocyte counts increased. As a result, it was concluded that Sc plays an important role in eliminating the pressure on the cellular activities in the early period of wound healing and continuance of the inflammatory process and wound healing within normal limits and also that this could be demonstrated with hematological analyses.
How Long-Term Intake of Sodium Fluoride (Naf) in Different Doses and 7,12 Dimethylbenz(A)anhtracene (Dmba) Affect the Erythrocyte Parameters in Rats
(Pakistan Agricultural Scientists Forum, 2019) Comba, B.; Oto, G.; Arihan, O.; Comba, A.; Uyar, H.
This study was aimed to search the effect of Sodium Fluoride (NaF) and 7,12 dimethylbenz(a)anthracene (DMBA) to erythrocyte fragility and parameters in rats. The nine groups were formed and each group contained 8 animals. Group 1: Control group (without any treatment). Group 2: Sesame oil (vehicle for DMBA). Group 3: 1 ppm NaF. Group 4: 15 ppm NaF. Group 5: 30 ppm NaF. Group 6: DMBA.Group 7: 1 ppm NaF + DMBA. Group 8: 15 ppm NaF + DMBA. Group 9: 30 ppm NaF + DMBA. Fluoride was added into the animals' drinking water in the form of NaF once a day, for 12 weeks. DMBA (10 mg/kg) was administered once a week and in a total of 12 weeks with oral gavage. Erythrocyte fragility was analyzed with osmotic hemolysis method and erythrocyte parameters with blood cell counter in whole blood. At 0.4% NaCl concentration groups 4, 5, 6, 8, and 9 showed significantly higher erythrocyte fragility values than control group (p <= 0.05). At 0.5% NaCl concentration, groups 6, 8 and 9 showed significant increase in erythrocyte fragility compared to other groups (p <= 0.05). The erythrocyte and hematocrit values were found significantly high in group 5 (p<0.001) and group 4 (p<0.01) while it was found low in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) compared to control group. Hemoglobin amount in group 5(p <= 0.01) and group 4 (p <= 0.05) were significantly higher than other groups. MCV and MCH in group 5 were significantly lower and these values in all groups with DMBA (6, 7, 8, 9) were determined significantly high compared to other groups. RDWC in group 5 (p <= 0.001) and group 4 (p <= 0.01) and in all groups with DMBA (6, 7, 8, 9) (p <= 0.05) was significantly increased compared to other groups. As a result, exposure to high doses of floride and DMBA may cause augmented erythrocyte fragility, abnormal erythrocyte parameters and anemia. Therefore, measures must be taken to protect the health of all living organisms in area exposed to high levels of fluoride and DMBA.
Investigation of Effects of Fluorosis on Some Minerals and Hormones in Sheep
(Chartered Inst. of Building Services Engineers, 2016) Comba, B.; Çinar, A.
Summary: This study was carried out to determine the effects of fluorosis on plasma fluor (F), serum calcium (Ca), phosphorus (P), magnesium (Mg), calcitonin (CT), intact parathyroid hormone (iPTH) and vitamin D3 (Vit D3) in sheep. A total of 75 Morkaraman sheep, 50 with chronic fluorosis (Fluorotic group) from Tendürek Mountain region (altitude about 2000 m) and 25 without any fluorosis (Control group) from Van (altitude 1700m) were used, aged 3-4 years. Data collected 50 sheeps with chronic fluorosis in the in East Anatolia, Van-Agri, Turkey, were compared with data from 25 without any fluorosis sheeps from the Van region. Plasma F levels were measured with spesific fluride electrode, serum Ca, P and Mg levels were photometrically determined; serum CT and iPTH levels were determined using chemiluminescent immunometric assay, serum Vit D3 level were analysed with HPLC method. Serum P and Vit D3 levels were lower in fluorotic group than in control group (p<0.001), while serum CT (p<0.01) and iPTH (p<0.001) levels were higher. The differences for Ca and Mg values were found statistically nonsignificant. The results obtained were after a long term exposure of sheep to high amounts of fluorine and supports occurrence of osteomalatic conditions. In conclusion, clean water should be given to animal and people who live in this region because of irreversible pathological situation occurs after fluorosis develops. Otherwise, Ca, P, Mg and Vit D3 supplementations has to be given at proper doses to prevent negative effects of fluoride on teeth and bones. © 2016, Chartered Inst. of Building Services Engineers. All rights reserved.
Investigation of the Localisation of Estrogen Receptor Alpha and Progesterone Receptor B in the Ovaries of Goats During and Out of the Breeding Season
(Wiley-blackwell, 2015) Uslu, S.; Yoeruek, M.; Mis, L.; Comba, B.; Sendag, S.; Wehrend, A.
Investigation of the Long-Acting Single-Dose Gnrh Vaccine (repro-Bloc™) Effect on the Germinative and Endocrine Testicular Function in Male Rats
(Wiley-blackwell, 2015) Eski, F.; Sendag, S.; Tasal, I.; Mis, L.; Comba, B.; Bulut, G.; Wehrend, A.
Is There a Relationship Between Serum Minerals (Ca, Mg) and Trace Elements (Cu, Fe, Mn, Zn) at Mating and Pregnancy Rates in Fat-Tailed Morkaraman Sheep
(Wiley-blackwell, 2015) Uslu, B. A.; Mis, L.; Gulyuz, F.; Comba, B.; Comba, A.; Kosal, V.; Ucar, O.