Browsing by Author "Coskun, Nuvit"

Now showing 1 - 3 of 3

Evaluation of the Relationship Between Inflammatory Reaction and Interleukins in Ovine Pulmonary Adenocarcinomas
(Urmia Univ, 2023) Karakurt, Emin; Coskun, Nuvit; Beytut, Enver; Keles, Omer Faruk; Dag, Serpil; Yilmaz, Volkan; Kurtbas, Emre
In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 -2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 -2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor -associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.(c) 2023 Urmia University. All rights reserved.
Seroprevalence of Bovine Leukemia Virus in Cattle and Buffaloes in the Border Provinces of the Eastern Anatolia Region, Türkiye: Insights Into the Eradication of Infection
(Urmia Univ, 2024) Babaoglu, Ali Riza; Oguz, Fatma Ertas; Yilmaz, Volkan; Coskun, Nuvit; Abounaaja, Fatima
Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, T & uuml;rkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a total of 1,033 serum samples were collected from 982 cattle and 51 buffaloes from the provinces of A & gbreve;r & imath; (n = 178), I & gbreve;d & imath;r (n = 252), Kars (n = 317), Van (n = 221), and Hakkari (n = 65) during 2021- 2022. In AGID and ELISA tests, seropositivity for BLV-specific antibodies was not detected in cattle and buffaloes from the mentioned provinces. This study revealed that BLV was not circulating in cattle and buffaloes in the easternmost border provinces of T & uuml;rkiye during the sampling period and contributed to determine the status of BLV in the mentioned region. Due to the presence of virus in other regions of T & uuml;rkiye and neighboring countries, Iran and Iraq, it is recommended to control animal movements, continue efforts to combat the transmission of the virus, and maintain control measures. (c) 2024 Urmia University. All rights reserved.
Striking Tick-Borne Virus Diversity and Potential Reservoirs Documented During One-Health Cross-Sectional Screening in Anatolia
(BMC, 2025) Dincer, Ender; Timurkan, Mehmet Ozkan; Oguz, Bekir; Ozan, Emre; Coskun, Nuvit; Kiziltepe, Semistan; Ergunay, Koray
An expansion of recently described human pathogenic tick-borne viruses from Central Asia toward Europe has been documented. Located on important bird migration routes, Anatolia is an intercontinental crossing hub with various climactic zones and with an abundance of endemic tick species. We sought to investigate tick-borne viruses utilizing a One Health approach encompassing host-removed ticks and host samples.MethodsWe collected host-attached ticks and accompanying plasma in 2023-2024 at locations in 20 provinces representing the 7 distinct geographical regions in Anatolia. The hosts comprised cattle, sheep, dogs, goats, and tortoises. The ticks were morphologically identified, processed in pools, and these pools, along with plasma from cattle, sheep and goats, were subjected to nucleic acid purification and complementary DNA synthesis. Viruses were screened by generic (nairovirus) and specific (Jingmen tick virus, JMTV; Tacheng tick virus 1, TcTV-1; Tacheng tick virus 2, TcTV-2; and Tamdy virus, TAMV) amplification assays and characterized by sequencing.ResultsA total of 93 animal plasma samples and 1265 samples from 11 tick species were screened in 192 pools. Crimean-Congo hemorrhagic fever virus (CCHFV) was detected in five tick species in ten pools (5.2%). Three distinct virus lineages, including Europe 1 and 2, as well as Africa 1, were noted. TcTV-1 was identified in 6 tick species in 12 pools (6.3%) and in a cattle plasma sample. Analysis of the nucleoprotein-encoding sequences revealed two separate virus clades, distinct from those reported from Asia and Europe. TAMV was identified in two tick species (1%). We further detected JMTV in 7 pools (3.6%), with sequences forming a new clade phylogenetically closer to viruses of Asian origin than local strains. Finally, highly divergent sequences of a novel nairovirus, forming a distinct group sharing ancestors with TcTV-1, TAMV, and pangolin/tick-associated nairoviruses, was observed in four pools (2%), comprising Haemaphysalis parva ticks.ConclusionsWe described a previously undocumented diversity of tick-borne viral pathogens, CCHFV, TcTV-1, and JMTV, in Anatolia. Possible animal reservoirs of TcTV-1 were identified. These pathogens and TAMV should be considered in the diagnostic workup of cases with symptoms associated with tick bites and in future surveillance efforts.