Browsing by Author "Dönmez, HH"

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A Biochemical and Histochemical Study on the Activity of Acid Peptide Hydrolase in the Hypothalamus at Some Periods of Starvation and Refeeding After Starvation
(Mrs Pushpa Agarwal, 2005) Temur, A; Karadag, H; Askerov, FR; Dönmez, HH
This study was carried out to determine acid peptide hydrolyse activity in the homogenate of the lateral (feeding centre) and ventro-medial (satiety centre) nuclei of hypothalamus and fractions of the cells (mitochondria, nucleus, cystocoele) in those centres of rats fed after being starved for a five day period. One hundred and sixty Wistar Albino rats were used in this study. The rats were starved for periods of 1, 3, 5 and 7 d. The rats starved for 5 d were then allowed to cat for periods of 5, 15 and 30 d. The activity of acid peptide hydrolyse was biochemically determined in the feeding and satiety centres. In addition, histochemical study was also done in these centres. It was observed that the activity of acid peptide hydrolyse became closer to the levels of the control group in the satiety centre on the 30th d of access to food after a 5 d starvation. It was concluded that the feeding centre was more sensitive to starvation than the satiety centre and the satiety centre was restored faster than the feeding centre.
Effects of Increasing Zinc Supplementation in Drinking Water on Growth and Thyroid Gland Function and Histology in Broiler Chicks
(M H Schaper Gmbh Co Kg, 2002) Dönmez, HH; Karsli, MA; Meral, I; Dönmez, N; Simsek, N
The aim of the study was to examine the effects of increasing zinc supplementation on growth, feed efficiency and thyroid function and histology in broiler chicks. Sixty new born male broiler chicks were randomly allotted into one of four treatment groups and fed for 60 d. Zinc (Zn) was added into drinking water at the levels of 0, 125, 500, and 1000 mg Zn/L. Body weight gain were significantly higher and feed efficiency were significantly lower in chicks supplemented with 125 mg Zn/L compared with chicks supplemented with 500 or 1000 mg Zn/L at the end of the experiment. Serum Zn concentration linearly increased with the increasing level of Zn intake. Serum triiodothyronine and thyroxine levels and the diameters of follicles of thyroid gland were significantly reduced with high levels (500 and 1000 mg Zn/L) of Zn intake at the end of the experiment. It was concluded that chick receiving 1000 mg Zn/L as ZnSO4 . 7H(2)O in drinking water showed signs of Zn toxicity.
Effects of Protein Deficiency on Testosterone Levels, Semen Quality and Testicular Histology in the Developing Male Rat
(Scandinavian Federation Laboratory Animal Science, 2003) Karaca, F; Dönmez, HH; Karsli, MA
The aim of the study was to evaluate the effects of protein deficiency on plasma testosterone levels, epididymal sperm characteristics and testicular histology in the developing male rat. Twenty four Wistar albino rats were used in the study and were fed with a diet variously containing 3%, 10% and 20% protein. Plasma testosterone levels were significantly lower in rats fed 3% protein when compared to rats fed 10% and 20% protein (P<0.05). Testicular weight in protein deficient rats (rats fed 3% and 10% protein) was significantly lower than that of a control group (20% protein) (P<0.05). No sperm was present in the epididymal duct lumen, but germ cells were observed in some duct lumens in rats fed 3% protein. Epididymal weight decreased significantly in rats fed 3% protein compared to rats fed 10% and 20% protein (P<0.05). The results of this study clearly show that protein deficiency in developing male rats adversely affects gonadal endocrine function, testicular histology and spermatogenesis.
Effects of Zinc Supplementation To Ration on Some Hematological Parameters in Broiler Chicks
(Humana Press inc, 2002) Dönmez, N; Dönmez, HH; Keskin, E; Çelik, I
The aim of the study was to examine the effects of zinc supplementation on some hematological parameters. Sixty newborn male broiler chicks were utilized in the study. Zinc (Zn) was added into drinking water at levels of 0, 125, 500, and 1000 mg/kg. In the study, there was no significant difference between control and Zn-supplemented groups in erythrocyte count, hemoglobin amount, hematocrit levels, total leukocyte count, and differential leukocyte % levels, but the alpha-naphthyl acetate esterase ANAE(+) lymphocyte rate significantly (p< 0.05) increased in the 125-ppm Zn-supplemented group compared with the control group. In conclusion, the data obtained may be beneficial in demonstrating the effects of zinc on, at least, these parameters.
Efficacy of Polyvinylpolypyrrolidone in Reducing the Immunotoxicity of Aflatoxin in Growing Broilers
(Taylor & Francis Ltd, 2000) Celik, I; Oguz, H; Demet, Ö; Dönmez, HH; Boydak, M; Sur, E
1. Protective action of an enzyme-linked polyvinylpolypyrrolidone (PVPP, Mycofix(R) Plus) against the immunosuppressive effect of afatoxins (AF) was evaluated by determination of peripheral blood TP-lymphocyte proportions and splenic plasma cell counts. Histological changes in lymphoid organs were also investigated by light microscopy. One-d-old broiler chicks (Hybro) received 2.5 mg/kg diet AF (83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1), 1.12% AFG(2)) with or without PVPP (3 g/kg diet) until 21 d of age. 2. When compared with the controls, AF treatment significantly decreased peripheral T-lymphocyte counts. AF caused a slight decrease in splenic plasma cell counts. The addition of PVPP to an AF-containing diet significantly increased T-lymphocyte counts. Splenic plasma cell counts were numerically intermediate between control and AF groups. 3. The dietary addition of PVPP to AF-free diet did not significantly alter either T-lymphocyte or splenic plasma cell counts.
Embryotoxicity Assay of Aflatoxin Produced by Aspergillus Parasiticus Nrrl 2999
(Taylor & Francis Ltd, 2000) Celik, I; Oguz, H; Demet, Ö; Boydak, M; Dönmez, HH; Sur, E; Nizamlioglu, F
1. The embryotoxicity of mixed aflatoxins (AF) and aflatoxin B-1 (AFB(1)) were evaluated by a modified chick embryotoxicity screening test (CHEST). Adverse effects on the early embryonic development of thymus and bursa of Fabricius were also investigated by light microscopy. AF consisted of 83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1) and 1.12% AFG(2). 2. A total of 448 fertilised laying hens' eggs were used. AF and AFB(1) were injected into the eggs at doses of 10, 100 and 1000 ng/egg. Embryonic developmental stages were evaluated according to the Hamburger-Hamilton scale (HH-scale). 3. The results showed that AFB(1) given at 10 ng/egg had a significantly (P<0.05) greater embryotoxic effect than AF given at a similar dose. The higher doses of both AF and AFB(1) caused higher embryonic mortality and also an increase in early deaths. 4. In the groups receiving 100 ng/egg AF and AFB(1) an abnormal development was seen, with a protruded central region, corresponding to the area pellucida of the blastoderm. No other developmental abnormality attributable to AF or AFB(1) was found.