Browsing by Author "Dalar, A."

Now showing 1 - 9 of 9

Analysis of Phytochemical Composition and Biological Activities of Helichrysum Pallasii (Sprengel) Ledeb Leaves
(Pamukkale University, 2023) Işık, N.; Mukemre, M.; Turker, R.S.; Zengin, G.; Dalar, A.
This study aimed at the investigation of in vitro biological activities (antioxidant and enzyme inhibition) and phytochemical composition of various extracts (ethanol maceration, aqueous infusion, and aqueous decoction) obtained from leaf organs of Helichrysum pallasii, which has been traditionally used as herbal tea and medicine in Eastern Anatolia. Experimental analysis showed that ethanol-based extract had higher total phenolic content (TPC: 103 mg GAE/gE) and showed superior antioxidant potentials (FRAP: 2205 μmol Fe2+/gE; ORAC: 2540 μmol Trolox Eq./gE; DPPH: IC50=0.58 mg/ml; CUPRAC: IC50=0.37 mg/ml; Phosphomolybdenum: IC50=1.34 mg/ml ve metal chelation: IC50=1.42 mg/ml) and enzyme inhibition (Acetylcholinesterase: IC50=1.49 mg/ml; Butyrylcholinesterase: IC50=1.98 mg/ml; Tyrosinase: IC50=0.68 mg/ml; Alpha-amylase: IC50=2.09 mg/ml; Alpha-glucosidase: IC50=0.51 mg/ml; and Pancreatic lipase: IC50=42.5 μg/ml) and contained higher amounts of phenolic (chlorogenic acid isomers and rutin) and fatty acid (palmitic, linoleic, and linolenic acids) compounds than traditional preparations (infusion and decoction). The current study's findings indicate that the leaves of the Helichrysum pallasii are a source of phytochemicals with strong antioxidant and enzyme inhibitory properties, implying that it could be a candidate for biotherapeutic agent research and development. © 2023 The authors.
Biological Activities and Chemical Composition of Xanthoria Lichens From Turkey
(Pamukkale University, 2021) Mukemre, M.; Zengin, G.; Turker, R.S.; Aslan, A.; Dalar, A.
This study presents the biopharmaceutical potential and bioactive composition of Xanthoria lichens (X. candelaria, X. elegans, X. parietina) that wildly grown and traditionally utilized as medicine in North Eastern Anatolia, Turkey, which has specific microclimatic and ecological zones. Chromatographic findings revealed significant levels of parietin compound (35 to 49 mg/g extract), low levels of various fatty acids and a volatile compound; α-terpinene in the extracts. The extracts exhibited pronounced antioxidant potential through reducing and scavenging mechanisms; FCR: 33-38 mg gallic acid equivalent, FRAP: 511-815 μ mol Fe2+, ORAC: 1032-1355 μ mol Trolox equivalent per gram extract, respectively and DPPH: IC50: 1.1-2.7, ABTS: IC50: 2-2.3, CUPRAC: IC50: 0.7-1.2, phosphomolybdenum: IC50: 2-2.9, metal chelation: IC50: 1.3-2.3 mg extract/ml, respectively. Concerning enzyme inhibitory activities, the extracts effectively suppressed the activity of acetylcholinesterase (IC50: 0.5-0.75 mg/ml), butyrylcholinesterase (IC50: 0.7-1.1 mg/ml), tyrosinase (IC50: 0.6-0.7 mg/ml), amylase (IC50: 1.7-2 mg/ml), glucosidase (IC50: 0.6-3 mg/ml) and lipase (IC50: 55-79 μg/ml) enzymes. These findings showed that Xanthoria lichens are dominated by parietin as the major key compound and high-tolerated lichen taxa towards to different ecological and climatic conditions. These lichens might be promising sources of novel antioxidant and enzyme inhibitory activities such as Xanthoria candelaria as antioxidant and antilipase, Xanthoria elegans as anticholinesterase, and Xanthoria parietina as antiamylase and antiglucosidase. © 2021 Acta Biomedica Scientifica. All rights reserved.
Gundelia Rosea Seed: Evaluation of Biopharmaceutical Potential and Bioactive Composition
(Elsevier, 2019) Dalar, A.; Zengin, G.; Mukemre, M.; Bengu, A. S.; Isler, S.
Gundelia species are among significant key medicinal plants extensively utilized in folk medicine of Middle Eastern countries. This study focused on researching the biopharmaceutical potency and bioactive compounds of Gundelia rosea seed. Hereby, traditional knowledge-based preparing methods (infusion and decoction) and ethanol-based lyophilized extracts obtained from Gundelia rosea seeds were assessed for (i) antioxidant capacities, (ii) enzyme inhibitory activities, (iii) HPLC-MS/MS and (iv) GC-MS studies. Phytochemical analysis revealed that ethanol extract which primarily compromised of mainly phenolics (4-Caffeoylquinic acid and luteolin hexoside) and several fatty acids (palmitic, stearic, oleic and linoleic acids), was superior to those of infusion and decoction extracts. Antioxidant activities findings revealed that ethanol extract contained a high level of total phenolics (55.3 mg Gallic acid Eq./g extract) and had high capacities of reducing (1683 mu mol Fe2+ and 214.1 mg Trolox Eq./g extract for FRAP and CUPRAC respectively) and radical scavenging (ORAC: 2241.9 mu mol, DPPH: 91.7 mg, ABTS: 141.2 mg Trolox Eq./g extract) and total antioxidant (Phosphomolybdenum: 1.39 mmol Trolox Eq./g extract) properties. The suppressive abilities of the extracts against selected isolated enzymes revealed that ethanol extract had pronounced levels of inhibitory activities against AChE (4.3 mg Galanthamine Eq.), BChE (3.4 mg Galanthamine Eq.), tyrosinase (120 mg Kojic acid Eq.), amylase (0.61 mmol Acarbose Eq.), glucosidase (11.91 mmol Acarbose Eq.) and lipase (53.4 mu mol Orlistat Eq.) per gram extract. Findings obtained within this study confirmed the traditional utilization of Gundelia rosea and suggest its potential as a novel candidate of biopharmaceutical agents for public health problems. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.
Health Attributes of Ethnic Vegetables Consumed in the Eastern Anatolia Region of Turkey: Antioxidant and Enzyme-Inhibitory Properties
(Elsevier B.V., 2016) Dalar, A.; Uzun, Y.; Turker, M.; Mukemre, M.; Konczak, I.
Background Four ethnic vegetables from the Eastern Anatolia region of Turkey, Malva neglecta Wallr., Plantago lanceolata L., Cichorium intybus L. and Eryngium bornmuelleri Nab. are commonly used by the local population for food preparation. This study aimed at understanding their potential health attributes. Methods Hydrophilic extracts obtained from roots, stems, leaves and flowers were evaluated for their antioxidant capacities [total phenolics (TP), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) assays] and suppression of two isolated key enzymes relevant to metabolic syndrome: α-glucosidase and pancreatic lipase. Phytochemical composition of extracts was investigated by high performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC-MS). Results The evaluated extracts exhibited pronounced antioxidant capacities, comparable to those of common spices and herbs, and effectively suppressed the activities of isolated α-glucosidase and pancreatic lipase enzymes. These activities correlated well with total phenolics contents. Plantago lanceolata was an effective inhibitor of α-glucosidase and C. intybus of pancreatic lipase enzyme. High performance liquid chromatography mass spectrometry analyses revealed the dominance of luteolin glycosides in P. lanceolata. The same compound was present in C. intybus, where it was accompanied by significant amounts of cichoric, chlorogenic and caftaric acid. Malva neglecta and E. bornmuelleri contained the lowest levels of phenolic compounds and exhibited the lowest antioxidant and enzyme inhibitory activities. Conclusions Among the investigated ethnic vegetables, P. lanceolata and C. intybus represent a valuable source of antioxidant phytochemicals of phenolic nature that modulated in vitro the activities of digestive enzymes. These ethnic food sources diversify diet and enhance health attributes of foods. © 2016 Korea Food Research Institute
The Phenolic Profile and Biological Activities of Common Scorzonera Species From Eastern Anatolia
(Pamukkale University, 2022) Babakr, S.H.; Emre, M.; Mukemre, M.; Dalar, A.
The present study focused on chemical composition and base therapeutic potential of common three Scorzonera species (Scorzonera mollis M.Bieb. subsp. mollis, Scorzonera papposa DC. and Scorzonera semicana DC), which have been utilized as food and medicine by local people of Eastern Anatolia for a long time. Comparative analytical studies were performed on ethanol-based extracts and traditional preparations (infusions) through chromatographic (HPLC-MS/MS) and reagent-based antioxidant and enzyme inhibitory assays. Results revealed that leaf extracts were rich in phenolics, particularly hydroxycinnamic acids that were confirmed by HPLC-MS/MS, chlorogenic acids and luteolin were the major phytochemical compounds of extracts. With regards to biological activities findings, it was determined that ethanol-based extracts showed better antioxidant activities and effectively suppressed the activities of α-glucosidase. In addition, both of the extracts were found as strong suppressive agents of pancreatic lipase activity so Scorzonera species were rich sources of bioactive compounds that able to deactivate reactive oxygen species and free radicals and as well as suppress the activities of α-glucosidase and pancreatic lipase. Finally, obtained findings reveal base data of Scorzonera species for researches that focused on novel candidates of nutraceuticals and biotherapeutics. © IJSM 2022.
Phytochemical Profile and in Vitro and in Vivo Anticonvulsant and Antioxidant Activities of Epilobium Hirsutum
(Pamukkale University, 2020) Dzhafar, S.S.; Dalar, A.; Mukemre, M.; Ekin, S.; Yıldız, D.; Yunusoglu, O.
This study presents the phytochemical profile and in vitro and in vivo anticonvulsant and antioxidant activities of Epilobium hirsutum, which has been traditionally used in the treatment of epilepsy by local people of Turkey. In vitro studies revealed that the extract contained a pronounced amount of phenolics (206.3±0.9 mg Gallic acid Eq/g extract) and exhibited significant levels of antioxidant (FRAP; 6226 µmol Fe2+/g extract, ORAC; 6593 µmol Trolox Eq/g extract, DPPH; IC50:33.8 ug/mL and metal chelation; IC50:114 ug/mL) and anticonvulsant (AChE; IC50:71.2 ug/mL, BChE; IC50:92.5 ug/mL, GABA-T; IC50:94.7 ug/mL) activities. In vivo studies shown that the extract exhibited high anticonvulsant activities. In addition, the extracts regulated the behaviour, locomotion and mental activities of the mice tested. Biochemical evaluation of the brain tissue revealed that the extract inhibited the production of MDA and stimulated the increasing of antioxidant enzyme levels, which suggest the possible antioxidative role of the extract that worked as neuroprotective agents by scarfing the free radicals produced through PTZ seizure inducer and attenuate convulsions. Moreover the extract regulated serum biochemical parameters, total antioxidant, total oxidant, and ischemia-modified albumin levels. Chromatographic studies were revealed that gallic acid principally might be the major contributor of anticonvulsant and antioxidant activities with the additive contributions of fatty acids and mineral compounds. Findings obtained from this study partially justified the traditional use of Epilobium hirsutum in the treatment of epilepsy and suggest potential use of the extract as industrial or pharmaceutical agent. © IJSM 2020.
Sium Sisarum L. Var. Lancifolium ( Bieb.) Thell a Traditional Spice From Eastern Anatolia: Chemical Composition and Biological Activities
(Pamukkale University, 2022) Mukemre, M.; Dalar, A.; Taylan, S.B.; Ertas, M.
Traditionally consumed food and spices are significant sources in the daily life diet and constitute a large portion of the cuisine in Eastern Anatolia in Turkey. However, limited data available necessitate further analysis of their chemical composition and health attributing properties. This study aims to present phytochemical composition and biological activities of Sium sisarum var. lancifolium, a commonly consumed spice and food species in the region. Analytical studies to date have revealed the presence of high levels of phenolics (chlorogenic acid and isoquercetin) and volatiles (a-terpinene, camphene, cyclohexene, carene and p-cymene), which exhibits significant potential of digestive enzyme suppressive and antioxidant abilities. Data collected in this study suggest the use of Sium sisarum plant to obtain nutraceuticals and/or biotherapeutic agents that are able to regulate oxidative stress and enzyme activities. © IJSM 2022.
Traditional Medicinal Plants of Van Province, Eastern Anatolia
(Nova Science Publishers, Inc., 2020) Dalar, A.; Mukemre, M.
This chapter presents significant ethnomedicinal knowledge of plant taxa by local people of Van Province of Eastern Anatolia. The richness of the local flora, the traditional and pastoral farming based lifestyle of local tribes, rurality of the province and limited opportunities of modern medical facilities resulted in a unique traditional medicinal system in the province. Through the richness of medicinal plant utilization, there is a limited report on documentation and inventory analysis on plant taxa used in the traditional medicine of Van Province. Hence, this study aimed to (I) record accumulation of the traditional medicinal plant knowledge of Van Province comprehensively to preserve the valuable local medicines knowledge, which has been threatened by urbanization, (II) analyze data to determine the most efficient plant taxa according to the ailment categories for drug discovery, (III) analyze local knowledge of plant materials that can assist pharmaceutical studies and (IV) reveal the origin(s) of traditional knowledge utilized in Van Province. Traditional medicinal plant determination surveys were conducted during seven years (2013-2019) through structured face-to-face interviews with the local healers who are well known in the province for their long practice in folk medicine (672 informants). Demographic characteristics of the participants, names of the local plants, plant part(s) used, preparation and utilization methods were all evaluated and recorded properly. Additionally, to assess the reliability of ethnomedicinal plant knowledge specified by the local people, data analysis (informant consensus factor; ICF) was calculated. 336 plant taxa belong to 165 genus (specifically Salvia, Allium, Centaurea, Plantago, and Hypericum) and 55 families (principally Asteraceae, Lamiaceae, and Apiaceae) were detected in the province. Data analysis revealed that hormonal disorders (ICF: 0.9881) was the most common ailment category in the province, followed by respiratory tract or muscle and joint pain (ICF: 0.9867), analgesic (ICF: 0.9864), and dermatological (ICF: 0.9856) categories. According to ICF analysis, it can be suggested that plant taxa used in Van folk medicine might serve as promising pharmaceutical agents. For instance, Artemisia absinthium, Campanula glomerata, Diplotenia turcica, Gundelia colemerikensis, Helichrysum plicatum, Lathyrus tuberosus, and Onopordum turcicum might be scientifically evaluated for their antidiabetic capacity. In respiratory tract ailments treatment, the following herbal medicines can be evaluated: Alcea digitata, Anthemis cretica, Mentha longifolia, Prunus divaricata, Rosa canina, Rosa pisiformis, Tanacetum balsamitoides, and Thymus kotschyanus. Also, for muscle and joint pain, Alcea sp., as an analgesic, Scutelleria sp., for dermatological disorders treatment, Alcea kurdica, Allium sp., Artemisia vulgaris, Cephalaria microcephala, Ferula haussknechtii, Medicago sp., Onobrychis sp., Rhus coriaria, Salvia verticillata, Plantago sp., Trifolium sp., and Cichorium intybus can be appraised. The rich local flora combined with the isolated traditional tribe culture in Van Province resulted in a unique plant-based traditional medicine knowledge that was mainly based on the trial and error method and passed down orally. The semi-nomadic lifestyle and rich local flora present in Van Province provided an extensive utilization of plant sources as significant folk medicines and suggest an important database for scientific pharmacological studies. © 2020 by Nova Science Publishers, Inc.
Traditional Uses of Ethnotoxic Plants in Van Province of Eastern Anatolia
(Nova Science Publishers, Inc., 2020) Mukemre, M.; Konczak, I.; Dalar, A.
This chapter provides a summary of ethnopharmacological information on toxic plants traditionally used by the indigenous people of Van Province, Eastern Anatolia Region of Turkey. The Van Province located in the eastern part of Turkey is situated in a green, fertile oasis amid rocky mountains. The landscape of the Province is overwhelmingly characterized by numerous highlands, lowlands, and valleys which, with their specific microclimate zones, provided suitable conditions for evolvement of vast numbers of endemic plant species. For centuries, these conditions facilitated an establishment of rural settlements with pastoral farming and extensive uses of local wild plants as food and medicine by the local population. The rich local flora combined with isolated traditional tribe culture of Van Province facilitated the development of a unique plant-based ethnotoxic knowledge, that was based on trial and error and is orally passed from generation to generation. Although the local flora is considerably utilized to date, no inventory of the used toxic plants has ever been created. Numerous plants used in traditional medicine are toxic, however, no reports on their phytochemical composition and description of traditional uses have ever been published. Therefore, the objective of this study is to record the traditional knowledge of medicinal uses of toxic plants of Van Province to preserve this valuable information. The ethnobotanical data was collected over five years period (2014-2019) based on structured face-toface interviews with local healers (240 informants) recognized for their long practice in traditional medicine using toxic plants. In total, 46 plant taxa that belonging to 19 plant families (principally Apiaceae and Ranunculaceae) were recorded. The study revealed that in traditional medicine, only 33 vernacular (common) names were in use to describe these 46 plant taxa. Out of the traditionally known 46 ethnotoxic plants only 15 are utilized for medicinal purposes. The application of toxic plants in folk medicine is based on (i) strict dose and (ii) utilization of the second fraction of decoction (or infusion). The highest degree of consensus among the traditional healers was recorded in regards to the effects of toxic botanicals on nervous system (ICF: 0.99). The ethnobotanical data collected in this study may provide valuable leads for the identification of novel and efficient pharmaceutical agents. © 2020 by Nova Science Publishers, Inc.