Browsing by Author "Dall'Acqua, Stefano"

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Analgesic Compounds From Scorzonera Latifolia (Fisch and Mey.) Dc
(Elsevier Ireland Ltd, 2010) Bahadir, Ozlem; Citoglu, Guelcin Saltan; Smejkal, Karel; Dall'Acqua, Stefano; Ozbek, Hanefi; Cvacka, Josef; Zemlicka, Milan
Ethnopharmacological relevance: A traditional mastic named yaki sakizi prepared from the roots of Scorzonera latifolia (Fisch. and Mey.) DC. is used as a folk remedy for treatment of pain in Turkish folk medicine. Aim of the study: To isolate and identify the compounds responsible for the antinociceptive activity of S. latifolia using bioassay-guided fractionation. Materials and methods: The methanolic extract of the S. latifolia roots was prepared and subjected to isolation procedures such as solvent-solvent partitioning and column chromatography. Writhing and tail-flick tests were used to determine the antinociceptive activity. Results: The n-hexane fraction of the S. latifolia root methanolic extract showed potent antinociceptive activity in both writhing and tail-flick tests. Three compounds were isolated from n-hexane fraction using bioassay-guided chromatographic purification. Isolated compounds were the triterpene taraxasteryl myristate, taraxasteryl acetate, and fern-7-en-3-beta-one, structures were elucidated by means of MS and NMR techniques. Both taraxasterol derivatives showed promising antinociceptive activity when compared to reference compounds. Conclusion: Results of the present study support the usage of S. (Walla in Turkish folk medicine. Both plant root extract and the isolated compounds showed promising antinociceptive activities. Our results suggested that antinociceptive activity of the plant extract is probably caused by the synergistic interaction of the isolated compounds. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Hepatoprotective and Tnf-Α Inhibitory Activity of Zosima Absinthifolia Extracts and Coumarins
(Elsevier, 2011) Bahadir, Ozlem; Citoglu, Gulcin Saltan; Ozbek, Hanefi; Dall'Acqua, Stefano; Hosek, Jan; Smejkal, Karel
Zosima absinthifolia (ZA) extracts and the coumarins (+)-columbianadin and (-)-deltoin were evaluated for their potential hepatoprotective and antiinflamatory effects in a CCl4-induced hepatotoxicity assay in rats and by the inhibition of TNF-alpha production on LPS-stimulated THP-1 macrophages, respectively. Both the ZA extracts and the coumarins showed hepatoprotective activity confirmed by monitoring the ALT/AST levels and by histopathological examination. The antiinflamatory activity, proved by the inhibition of TNF-alpha production, was found to be higher for the n-hexane root extract than for coumarins, suggesting synergic potential of the extract. The concentration of (-)-deltoin and (+)-columbianadin in extracts was determined by HPLC analysis. (c) 2011 Elsevier B.V. All rights reserved.
Phytochemical Analysis of Podospermum and Scorzonera N-Hexane Extracts and the Hplc Quantitation of Triterpenes
(Mdpi, 2018) Bahadir-Acikara, Ozlem; Ozbilgin, Serkan; Saltan-Iscan, Gulcin; Dall'Acqua, Stefano; Rjaskova, Veronika; Ozgokce, Fevzi; Smejkal, Karel
Previously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of -amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3--hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3--hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of -amyrin, 3-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae.