Browsing by Author "Deǧer, Y."

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Determination of the Status of Lipid Peroxidation and Antioxidants in Cattle Infected With Dictyocaulus Viviparus
(2008) Deǧer, S.; Deǧer, Y.; Ertekin, A.; Gül, A.; Biçek, K.; Ozdal, N.
Endoparasites cause significant economic losses and health problems in domestic animals. In this study, lipid peroxidation and the antioxidant status were investigated in the lung tissue taken from twenty cattle infected with Dictyocaulus viviparus and ten healthy cattle. Malondialdehyde superoxide dismutase, catalase, glutathione, vitamin C and beta-carotene were measured. In comparison to the control group, the concentration of MDA was high (p < 0.001), but the activities of Cu-Zn-SOD and CAT, and the concentration of GSH, vitamin C and beta-carotene were low (Cu-Zn-SOD, CAT, GSH, vitamin C, p < 0.001 and beta-carotene p < 0.05) in the parasite infected group. These results suggested that endoparasitic infection is among the major causes of oxidative stress. Lipid peroxidation was observed and the activities and concentrations of antioxidants systems were decreased in the lungs of cattle infected with Dictyocaulus viviparus.
The Effect of Draxxin Treatment on Blood Gases Levels of Montofon Calves With Pneumonia
(2010) Tanritanir, P.; Raǧbeth, C.; Deǧer, Y.; Ceylan, E.
The purpose of this study was to investigate examine changes in venous blood gases of Brown Swiss calves with pneumonia. For this purpose, 30 calves exhibiting having clinical sings of pneumonia and 20 healthy calves were used. Venous blood samples were collected from the healthy and diseased calves before and after treatment with Draxxin (tulathromycin-Pfizer) in order to determine pH, pCO2, pO2, tCO2 and HCO3 levels by Blood Gas Analyzer. Compared to the controls group, in the diseased animals, there was a significant increase of pCO2 and HCO3 levels, together with a significant decrease of pH and pO2 levels (p<0.05). One week after treatment, pH, pCO2 and HCO3 levels were significantly changed. Despite this change, these levels were still similar with other groups. Also, the venous blood pO2 level went back increased to be statistically the same as the healthy animals. The tCO2 level was not found statistically different among the three groups. It is concluded that pneumonia in calves impairs gas exchange, but that gas exchange improves rapidly after treatment. © 2010 Academic Journals Inc.
Evaluation of Oxidant/Antioxidant Markers, Cytokines, DNA Damage and Trace Mineral Levels in Goats Naturally Single and Co-Infected with Theileria Ovis and Anaplasma Ovis
(Alexandria University, Faculty of Veterinary Medicine, 2024) Mi̇s, L.; Deǧer, Y.; Oğuz, B.
The impact of co-infections with Theileria ovis and Anaplasma ovis on caprine health is very limited knowledge and is rarely documented in the literature. Türkiye is endemic for tick-borne diseases (TBDs), posing a risk of co-infections in goats. To evaluate the impact of single and co-infection with T. ovis and A. ovis on caprine health, four groups of goats were examined: healthy goats, goats infected with T. ovis, goats infected with A. ovis and goats co-infected with both species. Sera from blood samples was tested for oxidant/antioxidant markers [malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST)], cytokines [interleukins (IL)-6, IL-1, tumor necrosis factor-α (TNF-α)], DNA damage [8-hydroxy-2'-deoxyguanosine (8-OHdG)] and trace minerals [(copper (Cu), zinc (Zn), manganese (Mn), selenium (Se)]. MDA, 8-OHdG, TNF-α, IL-1 and IL-6 levels, CAT and GST enzyme activities were found to be significantly increased in both Theileria ovis and Anaplasma ovis single- and co-infections (P<0.05). In goats, single and co-infected with A. ovis showed a statistically significant increase in SOD enzyme activity compared to goats infected only with T. ovis (P<0.05). In goats co-infected with A. ovis and T. ovis, considerable decrease in GPx enzyme activity were observed (P<0.05). Zn and Se levels in single infected with A. ovis indicated a significant decrease. Also, Cu, Zn, and Se levels in co-infected animals decreased significantly (P<0.05). Significant increase in lipid peroxidation and antioxidant enzyme activity in goats with single and double infections indicates impaired erythrocyte membrane integrity due to oxidative stress. Moreover, increased proinflammatory cytokine concentrations in all groups indicate that a high degree of systemic inflammation is induced during parasitaemia. © 2024 Alexandria University, Faculty of Veterinary Medicine. All rights reserved.
Investigation of Thiol-Disulphide Homeostasis, Total Oxidant-Antioxidant and Ischaemia-Modified Albumin Levels in Dogs With Babesiosis
(Alexandria University, Faculty of Veterinary Medicine, 2023) Tarhan, M.; Deǧer, Y.; Oğuz, B.; Ozdek, U.
This study was designed to reveal the diagnostic efficiency of total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), thiol/disulphide homeostasis (TDH), albumin (ALB) and ischaemia-modified albumin (IMA) parameters in the assessment of oxidative stress in dogs with babesiosis. While the babesiosis group consisted of a total of 7 dogs with clinical and parasitological (ELISA) diagnoses of babesiosis, the control group consisted of 7 healthy dogs. Blood samples were taken from both groups. While the levels of TAC, TOC and ALB in the blood serums of the groups were measured using a commercial kit, the levels of total thiol (TT), native thiol (NT) and IMA were measured using the method. The OSI and disulphide (Ds) levels were calculated using the formula. When compared with the control group, TAC and ALB levels significantly decreased, whereas the levels of TOC and OSI elevated in the babesiosis group (p<0.05). No difference was found between the groups in terms of NT, TT, Ds, and IMA levels and the ratios of NT/TT, Ds/TT, and Ds/NT (p>0.05). A negative significant correlation was found between TAC and OSI and between Ds/NT and NT, TT and NT/TT (p<0.05; p<0.001, respectively). A significant positive correlation was found between NT and TT and NT/TT and between TT and NT/TT (p<0.001). The areas under the ROC curve of the variables TAC, TOC, OSI and ALB were found to be 0.918, 0.796, 0.980 and 1, respectively. The results showed that TAC, TOC, OSI and ALB performed diagnostically in the assessment of elevated oxidative stress in dogs with babesiosis. © 2023 Alexandria University, Faculty of Veterinary Medicine. All rights reserved.
Lipid Peroxidation and Antioxidant Potential of Sheep Liver Infected Naturally With Distomatosis
(2008) Deǧer, Y.; Ertekin, A.; Deǧer, S.; Mert, H.
The aim of this study was to assess the effects of natural distomatosis infections on sheep liver malondialdehyde (MDA) concentration, activities of enzymatic antioxidants (glutathione peroxidase (GPx), superoxide dismutase (Cu, Zn-SOD), catalase (CAT)) and concentrations of non-enzymatic antioxidants (reduced glutathione (GSH), vitamin C, and beta-carotene). Eighteen Akkaraman sheep naturally infected with Fasciola sp and Dicrocoelium dentriticum (D. dentriticum) and ten healthy Akkaraman sheep were included in the study Liver samples for the analysis of MDA, GPx, Cu, Zn-SOD, CAT, GSH, vitamin C, and beta-carotene and blood samples for the measurement of alanine aminotransferase and aspartate aminotransferase were collected immediately after sheep in the two groups were slaughtered. The concentration of MDA and activity of GPx in the group with distomatosis were higher than in the control group (P < 0.001). However, the Cu, Zn-SOD, CAT activities and the GSH, vitamin C concentrations in the infected group were significantly lower than in the control group (P < 0.001). The serum beta-carotene was not found to be statistically different in the two groups (P > 0.05). ALT and AST serum activities of the group with distomatosis were significantly higher in comparison to the control group (P < 0.001). In this study it was demonstrated that lipid peroxidation increased and activities or/and concentrations of antioxidant compounds were significantly changed in the liver of sheep with distomatosis.