Browsing by Author "Dede, S"

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Anti-Oxidant Activity in Measles
(Maney Publishing, 2005) Cemek, M; Bayiroglu, F; Çaksen, H; Cemek, F; Dede, S
Background: Measles leads to serious complications, especially in developing countries. Aim: Our aim was to evaluate the effect of oxidative stress on the enzymatic and non-enzymatic anti-oxidant status in children with measles. Methods: Levels of superoxide dismutase, glutathione peroxidase, catalase, ceruloplasmin, albumin, total bilirubin and uric acid were studied in 17 children with measles and 29 healthy controls. Results and conclusions: There was a statistically significant difference between the groups for all parameters. All of the anti-oxidant activities except for albumin level were increased in the study group. Both enzymatic and nonenzymatic anti-oxidant defence systems were affected in measles.
The Effect of Nigella Sativa (Black Seed) and Vit. E Plus Selenium in the Prevention of Liver Necrosis Experimentally Induced With Carbon Tetrachloride (Ccl4) in Rats
(Tubitak Scientific & Technological Research Council Turkey, 2003) Sahin, A; Yener, Z; Dagoglu, G; Dede, S; Oto, G; Alkan, M
The preventive effect of Nigella sativa (black seed) was investigated in rats with hepatic necrosis experimentally induced by carbon tetrachloride (CCl4). To compare the antioxidant effect of black seed, well-known antioxidants, vitamin E and selenium mineral, were used. This study was conducted on 40 rats that were divided into five groups of eight. Group A was separated as a control group and treated intraperitoneally (i.p.) with only liquid paraffin. Group B was administered i.p CCl4 in liquid paraffin. Group C was administered i.p. CCl4 in liquid paraffin and intramuscularly (i.m.) treated with vitamin E + selenium. Group D was administered i.p. CCl4 in liquid paraffin, i.m. treated with vitamin E + Se and fed ad libitum with standard rat pellets containing 10% black seed. Group E was administered i.p. CCl4 in liquid paraffin and fed ad libitum with standard rat pellets containing 10% black seed. Blood samples were drawn from the heart of the rats four times for the experimental groups and five times for the control group, one of which was at the beginning of the experiment. In blood samples, malondialdehyde (MDA), glutathione peroxidase (GSH-px), superoxide dismutase (SOD), alanine transaminase (ALT), aspartic transaminase (AST), intracellular glutathione (GSH) and ceruloplasmin analyses were performed. In group B blood samples, lipid peroxidation and antioxidant enzymes were significantly increased. The closest enzyme activities to those of the control group were found in group E. The least weight loss percentages were in group E. The least necrosis was detected in the liver samples of group D. Histopathological results were consistent with biochemical results. In conclusion, a liver necrosis preventive effect of vitamin E + selenium and black seed was observed.
Effect of Protein Deficiency on Macroelement and Trace Element Levels of Weanling Rats' Small Intestine and Liver Tissues
(Humana Press inc, 2005) Kilicalp, D; Dede, S; Belge, F; Tatar, M
Protein energy malnutrition has become a major health issue in developing countries. In the present study, the effect of protein deficiency on the small intestine and liver tissue content of macroelements and trace elements was investigated in weanling rats. Forty-five male weanling Wistar albino rats were divided into three groups. The control group (C) was fed a standard diet containing 25% casein, whereas the two experimental groups E1 and E2 consumed 12% and 3% casein, respectively, over a period of 45 d. The tissue samples were analyzed for zinc, copper, iron, manganese, calcium, and magnesium by atomic absorption spectroscopy. The protein-deficient groups showed increased levels of iron in both tissues and decreased manganese in small intestine tissue from the E1 group. No other differences were found for the other elements. These results suggest that protein deficiency might cause iron accumulation in the liver and intestine and decreases of manganese in the small intestine.
Effect of Short-Term Hypothermia on Lipid Peroxidation and Antioxidant Enzyme Activity in Rats
(Wiley-blackwell, 2002) Dede, S; Deger, Y; Meral, I
This experiment was carried out to determine the effect of short-term hypothermia on blood malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G-6-PD) concentrations in rats. Twenty Sprague-Dawley rats were used weighing 180-200 g and on average 3.5 months old. They were randomly divided into two experimental groups: control (without cooling) and hypothermic (with cooling). The rats of the hypothermic group were cooled by immersion into cold water (10-12 degrees C), and the control rats were immersed into water of body temperature (37 degrees C) up to the neck without using any anaesthetic or tranquilizer for 3 min Rectal body temperatures of both groups were measured and blood samples to analyse MDA, GSH, SOD, GSH, GSH-Px and G-6-PD were collected immediately after the treatment. It was found that the MDA level was higher and the GSH and G-6-PD levels were lower in the hypothermic group than those in the controls. There was no difference between the control or hypothermic group regarding SOD or GSH-Px levels. It is concluded that acute hypothermia increased the lipid peroxidation and decreased the GSH and G-6-PD levels in rats.
Effects of Gestation and Lactation on Lipid Peroxidation and Antioxidant System in Sheep
(indian veterinary Journal, 2003) Yur, F; Dede, S; Deger, Y; Mert, N
Pregnancy is accompanied by increased energy demands of many body systems. Production of reactive oxygen species (ROS) is proportional to the rate of oxygen consumption. Therefore, a concomitant increase in the activity of antioxidants during pregnancy could be expected (Mover-Lev and Ar., 1997). Malondialdehyde (MDA) is a major breakdown product split off from lipid peroxides (Mihailovic et al., 2000). The antioxidant systems protect the cells against lipid peroxidation which is the basis of many pathologic processes (Bray and Bettger, 1990). Superoxide dismutase (SOD) are metalloenzyme, and important initial component in the cellular defense against oxygen toxicity (Fridovich, 1986). Glutathione peroxides (GPx) are enzymes that catalyze the reduction of organic hydroperoxides or hydrogen peroxide by glutathione (Knapen, 2000). Antioxidant vitamins like E, C and A protect the cells from damage against free oxygen radicals. The present study examined the level of MDA and antioxidative substances such as SOD, GPx, GSH, vit E, C, beta-carotene, retinol, during the gestation and, lactation period of sheep.
Effects of Nigella Sativa and Vitamin E+se in Ccl4 Treated Rats
(indian veterinary Journal, 2004) Deger, Y; Sahin, A; Dede, S; Kilicalp, D; Cemek, M
Carbontetrachloride (CCl4), a selective hepatotoxic chemical agent has been utilized to produce liver cirrhosis in experimental animals (Parola et al., 1992). Vitamin E and Se are well-known liver preservative and hepatotrophic compounds (Zhang et al., 1996). Nigella sativa (black seed) is a seed of capsulated plants and belongs to the Ranunculaceae spp. Recently researches have shown the therapeutic effect of N. Sativa extracts such as bronchodilator, immunomodulative, antibacterial, hypotensive, choleretic, antitumoral, antifungal and anthelmentic (Salomi et al., 1992). The trace elements such as copper and zinc are co-factors of some enzymes, such as SOD which participate in antioxidant process by inhibiting reactive oxygen production (Bray and Bettger, 1990). The aim of this study was to determine the effect of N. sativa, well known therapeutic agent, vitamin E+Se administration on serum antioxidant vitamins (vitamin E, vitamin C, vitamin A, beta-carotene and retinol) and trace elements (Zn, Cu) concentrations on CCl4 treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats
(Wiley, 2003) Kanter, M; Meral, I; Dede, S; Cemek, M; Ozbek, H; Uygan, I; Gunduz, H
This study was designed to investigate the effects Nigella sativa L. (NS) and Urtica dioica L. (UD) on lipid peroxidation, antioxidant enzyme systems and some liver enzymes in carbon tetrachloride (CCl4)-treated rats. A total of 60 healthy male Sprague-Dawley rats were utilized in this study. The rats were randomly allotted into one of four experimental groups: A (CCl4 -only treated), B (CCl4 + UD treated), C (CCl4 + NS treated) and D (CCl4 + UD + NS treated), each containing 15 animals. All groups received CCl4 [0.8 ml/kg of body weight, subcutaneously, twice a week for 90 days starting day 1]. In addition, B, C and D groups also received daily intraperitoneal injections of 0.2 ml/kg NS or/and 2 ml/kg UD oils for 45 days starting day 46. Group A, on the other hand, received only 2 ml/kg normal saline solution for 45 days starting day 46. Blood samples for the biochemical analysis were taken by cardiac puncture from five randomly chosen rats in each treatment group at beginning, at 45th and at 90th day of the experiment. The CCl4 treatment for 45 days increased the lipid peroxidation and liver enzymes, and also decreased the antioxidant enzyme levels. NS or UD treatments (alone or combination) for 45 days starting day 46 decreased the elevated lipid peroxidation and liver enzyme levels and also increased the reduced antioxidant enzyme levels. Live weights of the rats decreased in group A, and increased in groups B, C and D. It is concluded that NS and UD decrease the lipid peroxidation and liver enzymes, and increase the antioxidant defence system activity in the CCl4-treated rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on Lipid Peroxidation, Antioxidant Enzyme Systems and Some Liver Enzymes in Ccl4-Treated Rats (Vol 50, Pg 264, 2003)
(Blackwell verlag Gmbh, 2003) Kanter, M; Meral, I; Dede, S; Gunduz, H; Cemek, M; Ozbek, H; Uygan, I
Effects of X-Ray Radiation on Lipid Peroxidation and Antioxidant Systems in Rabbits Treated With Antioxidant Compounds
(Humana Press inc, 2003) Deger, Y; Dede, S; Belge, A; Mert, N; Kahraman, T; Alkan, A
The aim of this study was to investigate the effects of supplemental antioxidant vitamins and minerals on lipid peroxidation and on the antioxidant systems in rabbits exposed to X-rays. The rabbits were divided into two experimental groups and one control group, each group containing seven rabbits. The first group (VG) received daily oral doses of vitamin E (460 mg/kg live weight) and vitamin C (100 mg/kg live weight). The second group (MG) was fed a mineral-enriched diet that contained 60 mg manganese chloride, 40 mg zinc sulfate, and 5 mg copper sulfate per kilogram of feed. The third group served as controls and received only a standard diet. Blood samples were obtained before and after the supplementation with vitamins or minerals, as well as before and after irradiation with a total dose of 550-rad X-rays. The blood samples were analyzed for their content of malondialdehyde (MDA), plasma vitamins C and E, retinol, reduced glutathione (GSH), and glutathione peroxidase activity (GPx). After irradiation, the control group showed increased levels of MDA and activity of GPx (p<0.05), whereas the levels of GSH, vitamin C, and vitamin E were decreased. In the VG, the concentration of MDA was lower (p<0.05), and the concentration of GSH and vitamins C and E were higher (p<0.05) when compared to controls. In the MG, the concentrations of MDA, GSH, vitamin C, and retinol were not affected by the mineral administration and radiation. The level of vitamin E in the MG increased with mineral administration (p<0.05), but decreased after irradiation (p<0.05). For the control group, the level of GSH was higher than in the two experimental groups. After irradiation, the VG animals had vitamin E and C levels that were higher than in MG and control groups (p<0.05). The activity of GPx was not affected by vitamin or mineral supplementation or by irradiation. We conclude that the supplementation with antioxidant vitamins and minerals may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by X-rays.
Enzyme Activity Changes in the Sera of Chickens Treated With Coccidiostatic Agents
(indian veterinary Journal, 2002) Deger, Y; Dede, S; Deger, S
Evaluation of Antioxidant Status in Children With Acute Bacterial Meningitis and Encephalitis
(Taylor & Francis Ltd, 2003) Çaksen, H; Dede, S; Cemek, M; Dulger, H; Cemek, F
Antioxidant status was investigated in children with acute bacterial meningitis and encephalitis to investigate the possible role of free radicals in children with meningitis and encephalitis. Our study included 16 children with acute bacterial meningitis, 13 with encephalitis, and 17 control subjects. Serum ceruloplasmin, uric acid, albumin, bilirubin superoxide dismutase (SOD), catalase, and glutathione peroxidase (GPx) levels were studied in all subjects within 6 h of admission. There was a statistically significant difference between the groups for all parameters except for serum uric acid. All antioxidant activities except for albumin level were increased in the study groups. Albumin level was higher in the control group than those of meningitis and encephalitis groups. When the values of meningitis and encephalitis were compared, there was a statistically significant difference between the groups for serum SOD, GPx, ceruloplasmin, and albumin. In conclusion, our study showed that serum SOD, GPx, catalase, and ceruloplasmin were higher in children with acute bacterial meningitis and serum SOD, GPx, catalase, ceruloplasmin, and total bilirubin levels were increased in children with encephalitis. These findings suggest that antioxidant status was almost similar in both acute bacterial meningitis and encephalitis conditions in childhood.
Investigation of Antioxidant Status in Children With Acute Otitis Media and Tonsillitis
(Elsevier Ireland Ltd, 2004) Cemek, M; Çaksen, H; Cemek, F; Bayiroglu, F; Dede, S; Dülger, H; Ustün, R
Objective: Antioxidant enzymes status was investigated in children with acute otitis media (AOM) and acute tonsillitis. The aim was to determine the effect of oxidative damage due to free radicals on the antioxidant enzymes status in children with AOM and acute tonsillitis. Methods: We measured erythrocyte levels of superoxide dismutase and glutathione peroxidase (GPx) and activity of serum catalase, ceruloplasmin, albumin and total bilirubin in 23 children with AOM, 27 with acute tonsillitis and 17 control subjects. Results: There was statistically significant difference between the groups for all parameters. ALL antioxidant activities except for albumin Level were increased in the studies groups. When the values of AOM and acute tonsillitis were compared, there was statistically significant difference between the groups for erythrocyte GPx. Conclusion: Our findings indicate that not only a few antioxidant enzymes but almost all antioxidant activities showed a significant enhancement in children with AOM and acute tonsillitis. (C) 2004 Elsevier Ireland Ltd. All rights reserved.
An Investigation on Β-Carotene and Vitamin C Levels in Natural and Fruit Yogurts
(indian veterinary Journal, 2004) Agaoglu, S; Dede, S; Deger, Y; Cemek, M
Levels of Some Vitamins, Trace Elements and Electrolytes in Rams With Rumen Acidosis and Laminitis
(indian veterinary Journal, 2003) Ceylan, E; Dede, S; Tas, A; Donmez, N; Atasoy, N; Keles, I
Feeding lambs with high grain rations (80-95% concentrate) generally increases the incidence of digestive disturbances related with rumen acidosis (Britton and Stock, 1986). Rumen acidosis varies from subacute to acute from, and is associated with various secondary disordes such as laminitis, polioencephalomalacia, rumenitis, and liver abscesses (Brent, 1976; Nocek, 1988). The present study was aimed to investigate the levels of some vitamins, trace elements and electrolytes in rams with laminitis.
Lipid Peroxidation and Antioxidant Status in Children With Acute Purulent Meningitis and Encephalitis
(Taylor & Francis Ltd, 2004) Çaksen, H; Cemek, M; Dede, S; Dulger, H; Cemek, F
In this study, lipid peroxidation and antioxidant status were investigated in children with acute bacterial meningitis and encephalitis. The aim was to determine whether there was a possible role of free radicals in meningitis and encephalitis in childhood. Our study included 16 children with acute bacterial meningitis, 13 with encephalitis, and 17 control subjects. Serum malondialdehyde (MDA), reduced glutathione (GSH), vitamin C, vitamin E, beta-carotene, and retinol levels were studied in all subjects within 6 h of admission. There was a statistically significant difference for serum MDA, GSH, and vitamin C between the groups. Serum MDA and vitamin C levels were higher, and serum GSH levels were lower in the study groups compared to the control group. Vitamin C levels were similar in both the encephalitis and control groups, but they were significantly lower in the children with encephalitis than the meningitis group. In conclusion, our study showed that serum MDA and GSH levels were affected in children with both meningitis and encephalitis, but vitamin C level was affected only in children with meningitis. Serum vitamin E, beta-carotene, and retinol levels were not changed in childhood meningitis and encephalitis.
Oxidant and Antioxidant Levels in Children With Acute Otitis Media and Tonsillitis
(Elsevier Ireland Ltd, 2005) Cemek, M; Dede, S; Bayiroglu, F; Çaksen, H; Cemek, F; Yuca, K
Objective: Recurrent episodes of acute otitis media (AOM) and acute tonsillitis (AT) are a common problem in infectious disorders during childhood and are major cause of morbidity in children. The organism maintains defense systems including nonenzymatic antioxidants such as Vitamins A, E and C and reduced glutathione (GSH) against reactive oxygen species (ROS). In the present study, Lipid peroxidation status and nonenzymatic antioxidant capacity were investigated in children with AOM and AT. Our aim was to compare the lipid peroxidation and responses of the body's antioxidant status in the closely associated infections such as AOM and acute tonsillitis. Methods: The study included 23 (14 mates, 9 females) children with AOM, 27 (14 mates, 13 females) with AT and 29 (16 mates, 13 females) healthy control subjects. The ages of the study and control subjects were between 2 and 7 years. Serum beta-carotene, retinol, Vitamin E, Vitamin C, and whole blood malondialdehyde (MDA) (as an indicator of lipid peroxidation) and GSH Levels were studied in all subjects. Results: There was a statistically significant difference between the groups for all parameters (P < 0.05). All of the antioxidant vitamins such as beta-carotene, retinol, Vitamin E, and Vitamin C levels were observed to be significantly decreased in the both patient groups. Nevertheless, GSH levels were also decreased in the patient groups. MDA levels were found to be higher in children with AOM and AT than in the healthy control subjects. When compared the AOM and AT groups, there was statistically significant difference between the groups for whole blood MDA (P < 0.05). Conclusion: Even though they seem to react in a similar way against ROS, in the cases of AOM and AT, it is possible to see the different approaches in these tissues for the oxidative stress. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Oxidation Products of Nitric Oxide and the Concentrations of Antioxidant Vitamins in Parasitized Goats
(Vysoka Skola veterinarni Farmaceuticka, 2002) Dede, S; Deger, Y; Kahraman, T; Deger, S; Alkan, M; Cemek, M
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E And C, beta-carotene and retinol) were determined spectrophotometrically in the blood serum of all Pats. The results were expressed as nitrate (mug/ml) 7.25 +/- 1.31-4.69 +/- 0.32; nitrite (mug/ml) 1.52 +/- 0.39-1.64 +/- 0.19; vitamin E (mg/100) 0.13 +/- 0.05-0.42 +/- 0.02; vitamin C (mg/100 ml) 1.49 +/- 0.26-1.46 +/- 0.15; retinol (g/100 ml) 201.51 +/- 15.69-234.081 +/- 45.15; beta-carotene (g/100 ml) 62.71 +/- 7.14-53.95 +/- 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.
Serum Profile of Calcium, Phosphore, Copper and Zinc in Healthy and Lice Infested Goats
(indian veterinary Journal, 2003) Dede, S; Deger, Y; Deger, S
Serum Trace Elements Status of Rabbits Supplemented With Nigella Sativa, Vitamins C and E, and Selenium Against Damage by N-Methyl
(Humana Press inc, 2002) Gündüz, H; Dede, S; Agaoglu, ZT; Atasoy, N; Mert, N
In this study, we investigated the effects of Nigella sativa, vitamins C and E, and selenium on the levels of trace elements in the serum of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-injected rabbits. The rabbits were separated into one control and three experimental groups, each consisting of eight rabbits. MNNG was administered to all rabbits at a dose of 20 mg/kg. Group A received a suspension of N. sativa, group B received a combination of vitamins C and E and selenium, and group C received MNNG without any additional treatment. Group D did not receive any treatment and acted as control. The concentrations of serum zinc, copper, and iron were determined for groups A, B, C, and D. The zinc levels were 155.3 +/- 25.8, 304.7 +/- 14.22, 117.2 +/- 27.9, and 87.0 +/- 8 mu/dL for groups A-D, respectively; copper was measured at 234.8 +/- 31.9, 214.3 +/- 14.2, 196.5 +/- 19.3, and 359.2 +/- 19.9 mu/dL and iron levels were 276.3 +/- 10.71, 260.8 +/- 7.15, 211.2 +/- 13.47, and 223.4 +/- 9.5 mu/dL, in the stated group order. There were statistically significant differences between groups (p < 0.05).
Studies on the Effects of X-Ray on Erythrocyte Zinc and Copper Concentrations in Rabbits After Treatment With Antioxidants
(Humana Press inc, 2003) Dede, S; Deger, Y; Mert, N; Kahraman, T; Alkan, M; Keles, I
The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.