Browsing by Author "Deger, M. Serdar"

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First Reports of Sarconema Eurycerca and Trinoton Anserinum in the Whooper Swan (Cygnus Cygnus) in Van, Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Oguz, Bekir; Kilinc, Ozlem Orunc; Deger, M. Serdar
Whooper swan Cygnus cygnus (Linnaeus, 1758) with wounded wing that found in the Ercis district of Van province was brought into Directorship of Wild Animal Protection of University of Yuzuncu Yil. Despite the surgical interventions, the whooper swan could not be rescued. Five lice in the feather and three nematodes in the heart were found at examination of the whooper swan. These lice specimens were identified as Trinoton anserinum (Fabricius, 1805). After the necropsy, nematodes were found in the heart of the whooper swan. Nematodes were identified as Sarconema eurycerca according to their morphological pecularities. Sarconema eurycerca have been reported for the first time in Whooper swan in Van, Turkey.
Genetic Analysis of Toxocara Spp. in Stray Cats and Dogs in Van Province, Eastern Turkey
(Walter de Gruyter Gmbh, 2018) Oguz, Bekir; Ozdal, Nalan; Deger, M. Serdar
Introduction: Toxocara canis and Toxocara cati are roundworms of dogs and cats. The purpose of this study was to investigate the infection caused by these ascarids in cats and dogs, using microscopic and molecular analysis methods. Material and Methods: Adult ascarids were gathered from the faeces of dogs and cats in Van province, in 2015-2016. Existing keys and PCR sequencing of the ITS-2 fragment were used to identify the morphological features of the parasite species. Results: It was observed that out of 20 adult ascarids, 17 and 3 were found to be Toxocara canis and Toxocara cati, respectively. The ITS-2 gene region was amplified by PCR to perform molecular analysis. Genotyping indicated that the dogs and cats were infected with T. canis and T. cati, respectively, and none had Toxascaris leonina. Conclusion: To the best of our knowledge, this is the first report on the molecular characteristics of adult ascaridoid nematodes from cats and dogs in Turkey. The molecular approaches established in this study enable molecular identification and genetic structure studies of the ascaridoids.
Molecular Identification Using 18s Ribosomal Rna of Sarcocystis Spp. in Bovine Minced Meat in Van Province, Turkey
(Ankara Univ Press, 2021) Oguz, Bekir; Deger, M. Serdar; Kosal, Sena
This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from Ipekyolu, Tusba, Edremit, Ercis, and Gevas districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic Local Alignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study.
Preliminary Studies on the Prevalence and Genotyping of Echinococcus Granulosus Infection in Stray Dogs in Van Province, Turkey
(Walter de Gruyter Gmbh, 2018) Oguz, Bekir; Ozdal, Nalan; Kilinc, Ozlem Orunc; Deger, M. Serdar
Introduction: Echinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method. Material and Methods: This study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method. Results: Taeniidae eggs were found in 10 (10%) out of 100 faecal samples. E. granulosus was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1). Conclusion: Dogs in Van area are primarily infected with the livestock genotype of E. granulosus, which is thought to be a potential zoonotic threat to humans.
Relationship Between Cardiac Injury, Selected Biochemical Parameters, Dic, and Hemogram Levels in Cattle With Theileriosis
(Polish Soc veterinary Sciences Editorial office, 2018) Kilinc, Ozlem Orunc; Ozdal, Nalan; Bicek, Kamile; Deger, M. Serdar; Yuksek, Nazmi; Yilmaz, Ali Bilgin; Oguz, Bekir
This study was undertaken to investigate the effects of theileriosis on the cardiovascular system in cattle. Blood samples were collected from 50 cattle naturally infected with Theileria spp. (before and after treatment) as well as from 30 non-infected cattle. Hematological parameters and the serum concentrations of total cardiac troponin I (cTnI), aspartate aminotransferase (AST), creatine kinase (CK), and creatine kinase MB (CK-MB) were measured. Also coagulation parameters, plasma levels of activated partial thrombo plastin time (APTT), prothrombin time (PT) and D-dimer were measured. The presence of anemia and the levels of cTnI, CK-MB, CK and AST indicated statistically significant differences between infected and healthy cattle, but no statistically significant differences between the two groups were found in DD levels at the end of the study. Reduction in anemia and reduced levels of cTnI, CK-MB, CK, AST, APTT and PT were also found in post-treatment analyses. The results indicate that anemia, coagulation disorders and hypoxia in theileriosis in cattle can induce myocardial tissue damage.