Browsing by Author "Deger, M.S."

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Molecular Detection and Phylogeny of Anaplasma Ovis in Goats in Van, Türkiye
(Springer Science and Business Media Deutschland GmbH, 2025) Oguz, B.; Deger, M.S.
One of the areas in Türkiye where animal husbandry is practiced extensively is the Eastern Anatolia Region. In the province of Van, which belongs to this region, depending on the sociocultural factors of the people, animal husbandry is carried out, especially in the form of small ruminant breeding. One of the most commonly reported intracellular tick-borne illnesses, Anaplasma ovis, infects ruminants worldwide and results in significant financial losses. The purpose of this study was to report the prevalence and phylogeny of this pathogen in goats that were obtained from Gevas, Van Province, Türkiye, via the msp4 gene. Blood samples were taken from the vena jugularis to the EDTA tubes from 205 randomly selected goats (apparently healthy) between April and June 2022. Using a pair of primers called MSP45/MSP43, the msp4 gene (major surface protein 4) of A. ovis was amplified from all the DNA samples via PCR. A total of 146 (71.2%) of the 205 samples amplified a fragment of 852 bp that was unique to the A. ovis msp4 gene. Sanger sequencing verified the presence of incomplete msp4 gene sequences, which were subsequently added to GenBank (PP625125–PP625128). None of the studied epidemiological characteristics (sex, age, infertility problem, history of abortion, or shelter conditions) were associated (P > 0.05) with A. ovis infection in the enrolled goats. Furthermore, there were variations in the prevalence of A. ovis in goats among the sampling sites. The analysis of the amplified partial msp4 sequence of A. ovis indicated that this gene is highly conserved, since all four sequences are phylogenetically closely related to each other and to the msp4 sequences amplified from other countries. The detection and phylogenetic analysis of A. ovis in this study have contributed to the existing knowledge of the parasite and provided valuable data on caprine anaplasmosis. Additionally, this research highlights the urgent need for further investigation and the implementation of targeted control measures in the region. © Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS) 2024.
Molecular Identification of Sarcocystis Species in Bovine Minced Meat Using Partial Cytochrome Oxidase Subunit 1 (Cox1) Gene in Van Province, Turkey
(Faculty of Veterinary Medicine, University of Forestry, 2022) Oguz, B.; Deger, M.S.
Sarcocystis species are obligate two-host protozoan parasites classified in the phylum Apicomplexa. Cattle are generally accepted to be the intermediate hosts for seven species, i.e., . S. cruzi, S. hirsuta, S. hominis, S. bovifelis, S. bovini, S. rommeli, and S. heydorni. Since it is not possible to differentiate between some species using amplification of the 18S ribosomal (rRNA) gen, the aim of this study was to reveal the molecular characterization of Sarcocystis species obtained from cattle minced meat amplifying partial cytochrome oxidase subunit 1 (Cox1) gene. Fifty DNA samples were used. Sequence analyzes of amplicons belonging to positive isolates were performed and their phylogenetic structures were investigated. While S. hominis was found in only one sample, it was molecularly confirmed that S. bovifelis was dominant species in other samples. We designed primer set in present study could not differentiate between S. bovini, S. rommeli and S. bovifelis species. Phylogenetic analyzes of isolates with GenBank records (OK041347-OK041353) were performed with similar isolates in the world. According to phylogenetic analysis, sequence of S. bovifelis (OK041347) was found closer to the isolates from cattle skeletal muscle in Argentina (KT900970 and KT900962). S. hominis (OK041352) isolate showed high genetic similarity to isolates from Netherlands and Italy (MK497840; MH021119). In conclusion, genetic characterization of S. bovifelis and S. hominis was performed for the first time in Van province of Turkey by partial cytochrome oxidase subunit 1 (Cox1) gene. © 2022 by the authors.
Molecular Investigation and Genotyping of Theileria Equi and Babesia Caballi in Horses in Mus Province, Turkey
(Hellenic Veterinary Medical Society, 2020) Oguz, B.; Özdal, N.; Deger, M.S.; Bicek, K.
Equine Piroplasmosis (EP) is a tick-borne disease caused by Theileria equi and Babesia caballi of the phylum Apicomplexa. In this study, 102 blood samples were randomly collected from the horses in Mus province of Turkey. PCR analysis, gene sequences, and phylogenetic analyses were carried out for detecting the presence and genotypic characteristics of species that cause piroplasmosis. Four (3.9%) of the 102 horses that were examined were found to be positive for T. equi, while B. caballi was not detected. Theileria equi isolates that were detected in the sequence analyses were found to be 100% identical to the isolates that were isolated from the horses in Turkey, the United States, and South Africa as well. In the phylogenetic analysis, all of the isolates were found to cluster with T. equi sequences in the genotype A. This study, in which we revealed intraspecies sequence heterogeneity of the parasite using the 18S rRNA gene region, provides important epidemiological data for equine piroplasmosis. However, we think that determining the characterization of genotypes that are common in different parts of our country is extremely important in terms of developing new diagnostic tools and vaccines. © 2020. All Rights Reserved.
Molecular Survey of Anaplasma Capra in Goats in Van Province, Eastern Türkiye
(Springer Science and Business Media Deutschland GmbH, 2024) Oguz, B.; Deger, M.S.; Al-Olayan, E.; El-Ashram, S.
Background: A newly discovered zoonotic infection carried by ixodid ticks, Anaplasma capra, affects a wide variety of hosts, including numerous mammals. A. capra most likely infects erythrocytes or endothelial cells in mammals. This study aimed to investigate the A. capra pathogen in goats in Türkiye’s Van province. Methods: A total of 200 goat blood samples were examined. Goat samples were subjected to partial amplification of the gltA gene fragment using a nested polymerase chain reaction. Results: A. capra DNA was detected in 0.5% of goat blood samples. Phylogenetic analysis of a partial gltA gene fragment showed that the Eastern Türkiye isolate, closely grouped with A. capra isolates reported from wild and domestic ruminants in France, Türkiye, and Kyrgyzstan, formed a distinct clade. Conclusions: This is the first report of A. capra in goats in Van province, Eastern Türkiye. © The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2023.