Browsing by Author "Deger, Y"

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Effect of Short-Term Hypothermia on Lipid Peroxidation and Antioxidant Enzyme Activity in Rats
(Wiley-blackwell, 2002) Dede, S; Deger, Y; Meral, I
This experiment was carried out to determine the effect of short-term hypothermia on blood malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glucose-6-phosphate dehydrogenase (G-6-PD) concentrations in rats. Twenty Sprague-Dawley rats were used weighing 180-200 g and on average 3.5 months old. They were randomly divided into two experimental groups: control (without cooling) and hypothermic (with cooling). The rats of the hypothermic group were cooled by immersion into cold water (10-12 degrees C), and the control rats were immersed into water of body temperature (37 degrees C) up to the neck without using any anaesthetic or tranquilizer for 3 min Rectal body temperatures of both groups were measured and blood samples to analyse MDA, GSH, SOD, GSH, GSH-Px and G-6-PD were collected immediately after the treatment. It was found that the MDA level was higher and the GSH and G-6-PD levels were lower in the hypothermic group than those in the controls. There was no difference between the control or hypothermic group regarding SOD or GSH-Px levels. It is concluded that acute hypothermia increased the lipid peroxidation and decreased the GSH and G-6-PD levels in rats.
Effects of Gestation and Lactation on Lipid Peroxidation and Antioxidant System in Sheep
(indian veterinary Journal, 2003) Yur, F; Dede, S; Deger, Y; Mert, N
Pregnancy is accompanied by increased energy demands of many body systems. Production of reactive oxygen species (ROS) is proportional to the rate of oxygen consumption. Therefore, a concomitant increase in the activity of antioxidants during pregnancy could be expected (Mover-Lev and Ar., 1997). Malondialdehyde (MDA) is a major breakdown product split off from lipid peroxides (Mihailovic et al., 2000). The antioxidant systems protect the cells against lipid peroxidation which is the basis of many pathologic processes (Bray and Bettger, 1990). Superoxide dismutase (SOD) are metalloenzyme, and important initial component in the cellular defense against oxygen toxicity (Fridovich, 1986). Glutathione peroxides (GPx) are enzymes that catalyze the reduction of organic hydroperoxides or hydrogen peroxide by glutathione (Knapen, 2000). Antioxidant vitamins like E, C and A protect the cells from damage against free oxygen radicals. The present study examined the level of MDA and antioxidative substances such as SOD, GPx, GSH, vit E, C, beta-carotene, retinol, during the gestation and, lactation period of sheep.
Effects of Nigella Sativa and Vitamin E+se in Ccl4 Treated Rats
(indian veterinary Journal, 2004) Deger, Y; Sahin, A; Dede, S; Kilicalp, D; Cemek, M
Carbontetrachloride (CCl4), a selective hepatotoxic chemical agent has been utilized to produce liver cirrhosis in experimental animals (Parola et al., 1992). Vitamin E and Se are well-known liver preservative and hepatotrophic compounds (Zhang et al., 1996). Nigella sativa (black seed) is a seed of capsulated plants and belongs to the Ranunculaceae spp. Recently researches have shown the therapeutic effect of N. Sativa extracts such as bronchodilator, immunomodulative, antibacterial, hypotensive, choleretic, antitumoral, antifungal and anthelmentic (Salomi et al., 1992). The trace elements such as copper and zinc are co-factors of some enzymes, such as SOD which participate in antioxidant process by inhibiting reactive oxygen production (Bray and Bettger, 1990). The aim of this study was to determine the effect of N. sativa, well known therapeutic agent, vitamin E+Se administration on serum antioxidant vitamins (vitamin E, vitamin C, vitamin A, beta-carotene and retinol) and trace elements (Zn, Cu) concentrations on CCl4 treated rats.
Effects of X-Ray Radiation on Lipid Peroxidation and Antioxidant Systems in Rabbits Treated With Antioxidant Compounds
(Humana Press inc, 2003) Deger, Y; Dede, S; Belge, A; Mert, N; Kahraman, T; Alkan, A
The aim of this study was to investigate the effects of supplemental antioxidant vitamins and minerals on lipid peroxidation and on the antioxidant systems in rabbits exposed to X-rays. The rabbits were divided into two experimental groups and one control group, each group containing seven rabbits. The first group (VG) received daily oral doses of vitamin E (460 mg/kg live weight) and vitamin C (100 mg/kg live weight). The second group (MG) was fed a mineral-enriched diet that contained 60 mg manganese chloride, 40 mg zinc sulfate, and 5 mg copper sulfate per kilogram of feed. The third group served as controls and received only a standard diet. Blood samples were obtained before and after the supplementation with vitamins or minerals, as well as before and after irradiation with a total dose of 550-rad X-rays. The blood samples were analyzed for their content of malondialdehyde (MDA), plasma vitamins C and E, retinol, reduced glutathione (GSH), and glutathione peroxidase activity (GPx). After irradiation, the control group showed increased levels of MDA and activity of GPx (p<0.05), whereas the levels of GSH, vitamin C, and vitamin E were decreased. In the VG, the concentration of MDA was lower (p<0.05), and the concentration of GSH and vitamins C and E were higher (p<0.05) when compared to controls. In the MG, the concentrations of MDA, GSH, vitamin C, and retinol were not affected by the mineral administration and radiation. The level of vitamin E in the MG increased with mineral administration (p<0.05), but decreased after irradiation (p<0.05). For the control group, the level of GSH was higher than in the two experimental groups. After irradiation, the VG animals had vitamin E and C levels that were higher than in MG and control groups (p<0.05). The activity of GPx was not affected by vitamin or mineral supplementation or by irradiation. We conclude that the supplementation with antioxidant vitamins and minerals may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by X-rays.
Enzyme Activities in Fascioliosis of Cattle
(indian veterinary Journal, 2005) Bicek, K; Deger, Y; Deger, S
Significant pathological disorder occur in the liver and biliary tracts in fascioliasis due to the migration of parasites. Enzymes released from the affected hepatic cells pass into the blood and bring about some changes in the early stages of the disease (Baysu et al., 1971). An increase in the activities of secretion of enzymes like GOT and ALP is believed to be an important factor in acute cellular disorders, however this factor becomes much more conspicuous in the obstruction of hepatic and biliary tracts (Benjamin, 1978). ALP, GOT, GPT and LDH enzymes are found in abundance in metabolically active tissues. Since GOT, GPT and LDH are enzymes controlling the fundamental reactions in the metabolism, their activities in the serum exacerbated due to massive tissue disruption (Kramer, 1980). This investigations deals with the changes occurred in GOT, GPT, LDH, GGT and ALP activities in the serum of cattle infected with fascioliosis.
Enzyme Activity Changes in the Sera of Chickens Treated With Coccidiostatic Agents
(indian veterinary Journal, 2002) Deger, Y; Dede, S; Deger, S
An Investigation on Β-Carotene and Vitamin C Levels in Natural and Fruit Yogurts
(indian veterinary Journal, 2004) Agaoglu, S; Dede, S; Deger, Y; Cemek, M
Oxidation Products of Nitric Oxide and the Concentrations of Antioxidant Vitamins in Parasitized Goats
(Vysoka Skola veterinarni Farmaceuticka, 2002) Dede, S; Deger, Y; Kahraman, T; Deger, S; Alkan, M; Cemek, M
The status of nitric oxide oxidation products and antioxidant vitamins were investigated in goats infected with endoparasites and blood parasites (Trichostrongylidae sp. + Protostrongylidae sp. + Eimeria sp. + Babesia sp.), in this study. Eighteen goats were naturally infected with these parasites and ten healthy goats served as controls which had been treated with antiparasitic drugs after parasitological examinations were carried out. The concentrations of nitric oxide oxidation products (nitrate, nitrite) and antioxidant vitamins (vitamins E And C, beta-carotene and retinol) were determined spectrophotometrically in the blood serum of all Pats. The results were expressed as nitrate (mug/ml) 7.25 +/- 1.31-4.69 +/- 0.32; nitrite (mug/ml) 1.52 +/- 0.39-1.64 +/- 0.19; vitamin E (mg/100) 0.13 +/- 0.05-0.42 +/- 0.02; vitamin C (mg/100 ml) 1.49 +/- 0.26-1.46 +/- 0.15; retinol (g/100 ml) 201.51 +/- 15.69-234.081 +/- 45.15; beta-carotene (g/100 ml) 62.71 +/- 7.14-53.95 +/- 3.82. In conclusion, nitrate concentrations of the infected group were higher than controls (p < 0.05) whereas vitamin E levels of the infected group were lower than the control group (p < 0.05). The concentrations of the other indices examined were not statistically different between groups. These results suggest that the parasitic infections have direct effects on the concentrations of vitamin E, an important antioxidant, and on the increase of nitrate levels which may result from the pathophysiological effects of the parasitic infections.
Serum Profile of Calcium, Phosphore, Copper and Zinc in Healthy and Lice Infested Goats
(indian veterinary Journal, 2003) Dede, S; Deger, Y; Deger, S
Studies on the Effects of X-Ray on Erythrocyte Zinc and Copper Concentrations in Rabbits After Treatment With Antioxidants
(Humana Press inc, 2003) Dede, S; Deger, Y; Mert, N; Kahraman, T; Alkan, M; Keles, I
The aim of this study was to investigate the effect of supplemental antioxidant vitamins and minerals on the erythrocyte concentrations of zinc and copper in rabbits after exposure to X-rays. The animals were divided into two experimental and one control group (CG). The first group (VG) was given daily oral doses of vitamins E and C; supplemental amounts of manganese, zinc, and copper were mixed with the feed and given to the second group of experimental animals (MG). Blood samples were taken from all groups before and after 4 wk of vitamin and mineral administration and after irradiation with a total dose of 550-rad X-rays. The administration of minerals caused the most significant increases of Zn and Cu. Even after irradiation, the zinc levels in the irradiated animals were higher than in the nonirradiated vitamin-supplemented animals (p<0.05). The results suggest that supplementation with antioxidant vitamins and minerals may have a protective effect against X-ray-induced damage.