Browsing by Author "Deger, Y."

Now showing 1 - 15 of 15

Activity, Acute and Sub-Acute Toxicity and Safety Assesment of the Hydroalcholic Root Extract of Diplotaenia Turcica
(Agricultural Research Communication Centre, 2018) Ozdek, U.; Basbugan, Y.; Yildirim, S.; Boga, M.; Firat, M.; Deger, Y.
Diplotaenia turcica is widely utilized in conventional treatment in the east of Turkey. Due to the insufficient data on the safety profile, the acute and sub acute toxicity of Diplotaenia turcica was determined. Furthermore, total phenolic content, flavonoid content and antioxidant activity of the extract were determined. The acute toxicity of hydroalcoholic root extract of Diplotaenia turcica (HREDT) was evaluated in mice after administration of single oral dose at the 5000 mg/kg (acute model) and rat after 28 days orally administration at the dose range of 250, 500 and 1000 mg/kg (subacute model). According to result, the LD50 value of HREDT was found to be greater than 5000 ing/kg. In sub acute toxicity study, no statistically significant differences were observed in the values of hematological and pathological parameters in comparison with control group. It was determined that the glucose, triglyceride, cholesterol and LDL levels exerted a significant effect depending on the HREDT doses. The results from the present study found out that HREDT did not produce any toxic effects or deaths in animals for both single and chronic administration. Additionally, HREDT showed moderate antioxidant activities and exhibited relatively notable total phenolic content.
Effects of Green Tea on Mineral Levels of Liver and Testis of Guinea Pigs Electromagnetic Field Emitted by Mobil Phones
(2009) Kiliçalp, D.; Dede, S.; Deger, Y.; Aslan, L.
It was reported that the effects of green tea on the mineral levels of testis and liver of Guinea pigs exposed to a 900 MHz electromagnetic field. Four experimental groups labeled as controls (Group A), irradiated (Group B), irradiated receiving green tea extract (Group C) and green tea only (Group D) were formed with seven randomly chosen animals of both sexes in each group. After exposure for one month, the animals were sacrificed by decapitation and testis and liver samples were collected for biochemical analysis. In female Guinea pigs irradiation with and without green tea as well as green tea alone caused significant changes of the iron levels in liver, but no significant changes of manganese, copper, zinc and the copper/zinc ratio. In males, irradiation caused significant increases of manganese and a decrease of the iron levels in liver and of manganese, copper, zinc in testis. Combined with green tea, electromagnetic radiation resulted in changes of manganese, iron, copper and copper/zinc ratio in liver and of manganese only in testis. Green tea alone changed the levels of hepatic iron, zinc and copper/zinc ratio and of testicular concentrations of iron and zinc. The highest levels of copper were found in the liver tissue of the irradiated animals that were also treated with green tea. From present findings we can state that testis tissue is more sensitive to electromagnetic radiation than liver tissue, showing greater changes in trace mineral metabolism. Green tea brings the trace element levels to near normal values; supporting the idea that green tea as a supplement has a protective effect against the damaging effects of electromagnetic radiation. © 2009 Academic Journals Inc.
Effects of Green Tea Upon Testis Weight and Epididymal Sperm Quality in Guinea Pigs Exposed To Electromagnetic Radiation
(Wiley-blackwell, 2012) Uslu, B. A.; Kilic, D. Kilicalp; Gulyuz, F.; Deger, Y.; Ucar, O.
Effects of Plantago Major Extract on Serum Levels of Antioxidant Vitamins and Minerals in Broiler
(Agricultural Research Communication Centre, 2018) Dede, S.; Bingol, N. T.; Kilinc, D. Kilicalp; Deger, Y.; Yoruk, I. H.; Ekici, P. Tanritanir; Karsli, M. A.
The aim of this study was to evaluate the effects of Plantago major liquid extract on serum antioxidant vitamin and mineral concentrations of broiler. The experiment consisted of the control and 2 treatment groups, composed of 28 Ross 308 broiler chicks (Total, 84). A basal (control) diet was formulated. P Major was added to the control diet as following: PM1 (5 g/kg feed), PM2 (10 g/kg feed). The chicks were feed for 42 days ad libitum. The serum micronutrients (Retinol, alpha-tocopherol, vitamin D, calcium, magnesium, iron, manganese, and zinc) levels were determined. The retinol, alpha-tocopherol, Fe, Mg, Mn levels were not affected, and the Zn levels decreased in the PM1 and PM2. The supplementation of P. major has affected the serum vitamin D and Zn levels for this study, and should be analyzed for the alterations on the metabolism in subsequent studies.
Effects of the Replacement of Soybean Meal With Pea as Dietary Protein Source on the Serum Protein Fractions of Broilers
(Facta-fundacio Arnco Ciencia Tecnologia Avicolas, 2016) Bingol, N. T.; Dede, S.; Karsli, M. A.; Deger, Y.; Kilinc, Kilicalp D.; Cetin, S.
The aim of this study was to determine the effects of the replacement of different levels of protein derived from soybean meal with that from peas in broiler diets on serum protein fractions. A corn-soybean meal basal diet was formulated as the control diet (Control=C) (NRC, 1994), and then pea was added to the control diet to replace 20% (P20) or 40% (P40) of the crude protein of the control diet. The diets were randomly fed to 12 pens per treatment, each housing five birds, for 42 days. Blood samples were collected from 36 birds (3 birds x 4 pens x3 treatments) and the serum protein fractions were separated. Gamma-globulin percentage was higher in group P20 compared with C and P40 groups. Total protein, beta-globulin, and gamma-globulin concentrations were significantly higher in group P20 compared with those of both control and P40 group (p<0.05).
The Impact of Chitosan on Oxidative Stress in Liver of Rats Loaded With Lead Acetate
(Hellenic veterinary Medical Soc, 2022) Ozdek, U.; Toz, H.; Komuroglu, A. U.; Mis, L.; Deger, Y.
This study was conducted to determine the impact of chitosan on lead (Pb)-induced hepatotoxicity. Thus, lead acetate was administered intraperitoneally (50 mg/kg for 5 days) and chitosan was given as 200 mg/kg via oral gavage for 28 days. When the trial was terminated, it was determined that aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in serum as well as Pb, 8-hydroxy-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels and catalase (CAT) activity in liver tissue significantly increased, however reduced glutathione (GSH) and ceruloplasmin (Cp) levels in liver and high density lipoproteins (HDL) in serum had a significant decrease in the Pb group when compared to the control group. The administration of chitosan significantly prevented Pb-induced changes in serum liver enzyme activities, 8-OHdG and MDA levels. In addition, chitosan showed a statistically insignificant effect on reduced GSH, Cp, HDL levels and CAT activity. Accordingly, administration of chitosan can strengthen the antioxidant defence system of liver tissue and may decrease oxidative stress.
Investigation of the Effects of Carrying Heavy Load on Prooxidation/Antioxidant Status and Vitamin D3 in Healthy Horses
(Academic Journals inc, 2009) Ceylan, E.; Dede, S.; Deger, Y.; Yoeruek, I.
The aim of the study was to investigate the effect of carrying heavy load for a long time on lipid peroxidation (MDA: malondialdehyde), NO2 (nitrite), NO3 (nitrate), antioxidants (GSH: reduced glutathione, retinol, alpha-tocopherol) and vitamin D-3 in healthy horses. Blood samples from seventeen native 3-5 years age and 450-500 kg live weight Anatolian horses carried a load which comprised at least 30% of their body weight and for 4 h on mountainous terrain (hard working) were evaluated. Blood samples were collected in the morning before the animals started to carrying load and immediately after they finished carrying (working). It is observed that the level of MDA, NO2 and NO3 increased significantly (p<0.05) after working. While GSH concentration, increased after working; levels of retinol, alpha-tocopherol and vitamin D-3 levels decreased significantly (p<0.05). On the other hand, the vitamin D-3 levels were affected by hard working as other lipid soluble vitamins. There were a correlation between the physiological response to hard-working and some oxidant markers in healthy-hard working horses. These observations provide evidence that hard-working increases oxygen consumption and cause a disturbance of intracellular prooxidant-antioxidant homeostasis.
Serum Biochemical Profile and Protein Fractions in Cattle With Theileriosis
(Ecole Nationale veterinaire Toulouse, 2014) Dede, S.; Altug, N.; Deger, Y.; Ozdal, N.; Ceylan, E.
Theileriosis encountered frequently in Van region and causes huge economic losses in cattle, sheep and goats. This study was carried out to examine the serum biochemical profile and serum protein fractions, determined before and 7 days after buparvaquone treatment, in 28 cattle, diagnosed to be infected with T. annulata and in 15 healthy control animals from the same region. The ALT (alanine amino transferase) and ALP (alkaline phosphatase) activities and the concentrations of bilirubin and urea were markedly increased in sera from infected animals whereas the concentrations of glucose, minerals (Na, K and Ca), total proteins, albumin and of a-globulins and the albumin/globulin (A/G) ratios were significantly depressed compared to the controls. In addition, buparvaquone treatment succeeded in alleviating the serum biochemical anomalies. These results show that liver and kidney failures occur during theileriosis leading to a global protein deficiency and that serum biochemistry and serum protein analysis may allow biochemical evaluation of treatment.
Serum Concentration of Copper, Zinc, Iron, and Cobalt and the Copper/Zinc Ratio in Horses With Equine Herpesvirus-1 (Biological Trace Element Research Doi: 10.1007/S12011-007-0023-y)
(2008) Yörük, I.; Deger, Y.; Mert, H.; Mert, N.
Serum Copper, Zinc, and Calcium Concentrations in Lice-Infested Sheep
(2002) Deger, Y.; Dede, S.; Deger, S.
The purpose of this study was to investigate changes in serum concentration of copper, zinc, and calcium in sheep naturally infested with lice (Bovicola caprae, Linognathus africanus, Linognatus ovillus, and Linognattus pedalis). Twenty sheep naturally infested with lice and 20 healthy sheep were used as subjects. Blood samples were collected from the sheep before and 8 and 15 d after treatment with Avermectin, a veterinary antiparasitic drug. The samples were analyzed for their serum copper, zinc, and calcium concentrations by atomic absorption spectrometry. The concentrations of these elements in the infested animals were lower than in the healthy controls, mainly because the general condition of the affected sheep was poor. When the infested animals were treated with an ectoparasitic drug, the serum levels of the studied elements rose to normal ranges while the health of the animals improved.
Serum Cytokine and Vitamin Levels in Experimental Diabetic Rats
(2011) Yazar, M.; Deger, Y.; Yur, F.
It is considered several cytokines are effective in pathogenesis and theraphy of diabetes mellitus. The aim of this study was to investigate serum concentrations of interleukin-1 β, interleukin-2, interleukin-6 and interleukin-10 cytokines thought to have relationship with diabetes mellitus and serum retinol, α-tocopherol and vitamin D3 levels notified they have constructive effects on diabetes in streptozotocyne induced diabetic rats. Male Wistar-Albino rats between 7-8 weeks old and weighing 180-210 g were used. Streptozotocin induced and blood glucose levels were found between 230-390 mg dL1 diabetic 25 rats formed the study group and 15 healthy rats constituted the control group. Serum cytokine levels in both groups were evaluated by solid phase sandwich ELISA method and serum vitamin levels were established by HPLC. Serum interleukin-1 β, interleukin-6 and interleukin-10 levels were not sufficient to constitute a statistical importance between groups. However, in diabetic group decreased interleukin-2 levels (p<0.05) were established. Besides, decreased retinol and vitamin D3 levels (p<0.05) were detected in diabetic group but reduction in levels of α-tocopherol did not reach statistical significance. In conclusion, decreased serum interleukin-2 levels established in diseased group may indicate this cytokine has a part in the pathogenesis of diabetes mellitus and reduced vitamin levels may exhibit they used for disposal of free oxygen radicals. Besides, increased vitamin D3 consumption may be due to its disposal in T cell regulation. © Medwell Journals, 2011.
Serum Total and Lipid-Bound Sialic Acid Concentrations in Sheep With Natural Babesiosis
(veterinarni A Farmaceuticka Univerzita Brno, 2007) Deger, Y.; Mert, H.; Dede, S.; Yur, F.; Mert, N.
The aim of this study was to determine the serum concentrations of total and lipid-bound sialic acid in sheep naturally infected with Babesia ovis before and after treatment. Thirty diseased sheep and ten control animals were used. Babesia infection was confirmed with Giemsa's staining of blood smears. Compared to the control animals (1.351 +/- 0.100 mmol center dot l(-1); 0.385 +/- 0.001 mmol center dot l(-1)), a marked increase of serum total and lipid bound sialic acid concentrations (1.929 +/- 0.101 mmol center dot l(-1); 0.479 +/- 0.004 mmol center dot l(-1), respectively) was obtained in infected sheep (p < 0.05). One week after treatment, serum total and lipid bound sialic acid concentrations significantly decreased (1.554 +/- 0.005 mmol center dot l(-1); 0.411 +/- 0.02 mmol center dot l(-1), respectively) (P < 0.05). Despite this decline, total and lipid bound sialic acid concentrations of treated animals were still significantly increased in sera compared to the control animals (p < 0.05). When the infected animals were treated with an antiparasitic drug (diminazene aceturate), the serum total and lipid bound sialic acid levels had a tendency to decrease to the normal ranges. It can be concluded that Babesia ovis infection induced marked and persistent elevations of serum total and lipid-bound sialic acid concentrations, suggesting that these indicators would indirectly promote the invasion and presence of the parasite in the host.
Total and Lipid-Bound Serum Sialic Acid and Hematological-Biochemical Gas Changes in Dogs With Dirofilariosis
(Hellenic veterinary Medical Soc, 2022) Kesici, H.; Deger, Y.; Oguz, B.; Ozdek, U.
The measurement of serum sialic acid concentration is of importance to indicate damage in cells or tissues. Dirofilaria immitis causes cardiovascular, pulmonary, hepatic and kidney lesions. Therefore, present study was planned to investigate the potential changes in the levels of serum total sialic acid (TSA), lipid-bound sialic acid (LSA) and hematological-biochemical parameters in the dogs naturally infected by Dirofilaria immitis.The patient group included the 7 dogs clinically and parasitologically (Modified Knott and PCR) diagnosed with Dirofilaria immitis while 7 healthy dogs were assigned as the control group. The biochemical parameters, blood gas parameters and hematological parameters were tested in the blood samples taken properly according to the guidelines from all the study animals using automated biochemistry analyzer, blood gas analyser and automated hematology analyzers, respectively. Serum TSA and LSA levels were measured spectrophotometrically using Sydow and Katapodis methods, respectively. Compared with the healthy group, the dogs diagnosed with dirofilariosis were found to have significantly increased levels of serum cardiactroponin, triglycerides, VLDL, LDL, BUN, urea and creatinine and enzymatic activities of CK-MB, ALT, AST, ALP and LDH, and statistically significantly decreased levels of cholesterol and HDL (p<0.05). A significantly decreased level of RBC and a significantly increased level of WBC was determined in the dogs with dirofilariosis (p<0.05). The differential leucocyte count test of the dogs with dirofilariosis indicated statistically significantly increased eosinophil count (p<0.05). In the dogs with dirofilariosis, the decreases in the levels of pCO(2), HCO3 and BE in venous blood were statistically significant (p<0.05). The levels of serum TSA and LSA of the dogs with dirofilariosis were found statistically significantly higher than the healthy group (p<0.05). As a conclusion, statistically significant differences were identified between the dogs with dirofilariosis and healthy dogs in terms of sialic acid levels and certain biochemical, venous blood gas and hematological parameters. On the other hand, the present study is the first to investigate the serum sialic acid levels in the dogs with dirofilariosis.
Trace Element Levels in the Serum and Liver of Sheep Infected With Fasciola Hepatica
(indian veterinary Journal, 2007) Deger, Y.; Deger, S.; Mert, H.
The aim of this study was to investigate the changes in the serum and liver concentration of copper, zinc, manganese and iron in the sheep naturally infected with Fasciola hepatica.
Trace Elements Level in the Serum and Liver of Sheep Infected With Fasciola Hepatica
(2007) Deger, Y.; Deger, S.; Mert, H.