Browsing by Author "Demir, Derya Cay"

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Determination of Some Trace Elements and Heavy Metal Levels (Cu, Mn, Mg, Fe, Zn, Co, Pb, and Cd) in Blood Serum of Patients With Lip and Oral Cavity Cancers
(Polish Society Magnesium Research, 2023) Demir, Derya Cay; Demir, Halit; Bozan, Nazim; Belli, Seyda; Demir, Canan
This study investigated copper (Cu), manganese (Mn), magnesium (Mg), iron (Fe), zinc (Zn), cobalt (Co), lead (Pb) and cadmium (Cd) levels in the blood serum of patients with lip and oral cavity cancer. Our study aims to determine the relationship of some trace elements and heavy metals with lip and oral cavity cancer. Blood serum results of 21 individuals with lip and oral cavity cancer were compared with those in the control group consisting of 30 volunteer and healthy subjects. The serum levels of trace elements and heavy metals (Cu, Mn, Mg, Fe, Zn, Co, Pb and Cd) were determined by atomic absorption spectrophotometry at Yuzuncu Yil University Central Research Laboratory (Spectrometer: Thermo Scientific C103500100, China). In this study, when the descriptive statistics and comparison results for Cu, Mn, Mg, Fe, Zn, Co, Pb, and Cd were examined, the difference between the patient and healthy group means was found to be statistically significant (p<0.05). According to these results, Zn, Fe, Mn, Mg, and Cu levels which are essential trace elements for the human body, were significantly lower in patients than in healthy groups. On the other hand, Cd, Co, and Pb levels which are harmful and toxic to the human body were significantly higher in patients than in healthy persons (p<0.05). In conclusion, deficiencies of some trace elements, such as Cu, Mn, Fe, Zn, and Co, were detected in the blood serums of patients with lip and oral cavity cancer. Deficiencies of trace elements in patients with lip and oral cavity cancer may result from excessive nutrient con-sumption or malnutrition of cancer patients, in addition to which trace element and heavy metal levels (Cu, Mn, Mg, Fe, Zn, Co, Pb and Cd) can affect the etiopathogenesis of the disease.
Molecularly-Imprinted Pumice as a Selective Solid-Phase Extraction Adsorbent for Kojic Acid Determination in Cosmetic Samples
(Pergamon-elsevier Science Ltd, 2025) Zengin, Adem; Karakoyun, Necdet; Demir, Derya Cay; Bilici, Mustafa
Herein, a highly sensitive molecularly-imprinted pumice (MIP-PMC) was tailored by employed the surface imprinting method and utilized as a solid-phase extraction (SPE) adsorbent for the fast and selective separation of kojic acid (KA) from cosmetic samples. The MIP-PMC was characterized in a systematic manner and found it to be a well-grown polymer layer on the PMC. The adsorption capacity of MIP-PMC was 89.9 mg/g, with an equilibrium adsorption time of 30 min. Moreover, the imprinting factor of MIP-PMC towards KA was found to be 3.28, which further demonstrated its evident selectivity for KA. Under optimized conditions, KA was successfully isolated from cosmetic products using MIP-PMC and quantified via ultraviolet-visible (UV-vis) spectrophotometry. Method validation demonstrated excellent linearity within the range of 0.04-400 mg/L, satisfactory precision (less than 5 %), and high recoveries (98.0-100.4 %). Furthermore, the method exhibited a low limit of detection (LOD) of 12.8 mu g/L with a preconcentration factor of 4. Moreover, the sensitivity of the developed method was also checked by using HPLC and the method has comparable analytical performance. The findings indicate that the integration of MIP-PMC based SPE with UV-vis spectrophotometry offers a highly efficient approach for sample pretreatment and the accurate determination of KA in real samples.
Oxidative and Antioxidative Biomarker Profiles in Neonatal Hypoxic-Ischemic Encephalopathy: Insights for Pathophysiology and Treatment Strategies
(int Scientific information, inc, 2024) Aycan, Nur; Demir, Derya Cay; Yurekturk, Eyyup; Basaranoglu, Murat; Karaman, Serap; Tuncer, Oguz
Background: Neonatal hypoxic-ischemic encephalopathy (HIE) is a significant cause of perinatal and postnatal morbidity and mortality worldwide. Catalase (CAT) activity detection is used to determine levels of inflammation and oxidative stress. Glutathione (GSH) is the most critical non-enzymatic endogenous antioxidant. Lipid peroxidation levels marked after hypoxia can be detected based on the level of malondialdehyde (MDA). Ischemia-modified albumin (IMA) is considered a biomarker for cardiac ischemia and is known to increase in the liver, brain, and kidney in states of insufficient oxygenation. We aimed to explain the results and relations between the oxidant and antioxidants to detail oxidant-antioxidant balance and cellular mechanisms. Material/Methods: Serum levels of IMA and MDA, as an oxidative stress marker, and CAT and GSH, as antioxidant enzymes, were measured in first blood samples of 59 neonates diagnosed with HIE, with pH <7, base excess >12, and APGAR scores. Results: Neonates who were >= 37 weeks of gestation and had hypoxia were included. Compared with healthy newborns (n=32), CAT was statistically significantly lower in the hypoxia group (P=0.0001), P =0.0001), while MDA serum levels were significantly higher in neonates with hypoxia (P=0.01). P =0.01). There was no difference between hypoxic and healthy neonates in GSH and IMA measurements (P=0.054, P =0.054, P =0.19 respectively). Conclusions: HIE pathophysiology involves oxidative stress and mitochondrial energy production failure. Explaining the pathways between oxidant-antioxidant balance and cell death, which explains the pathophysiology of HIE, is essential to develop treatment strategies that will minimize the effects of oxygen deprivation on other body organs, especially the brain.