Browsing by Author "Demir, Ibrahim"

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Astragalus Guzelsuensis (Fabaceae: Galegeae), a New Species From Turkey
(Finnish Zoological Botanical Publishing Board, 2009) Ghahremaninejad, Farrokh; Behcet, Luetfi; Demir, Ibrahim
Astragalus guzelsuensis F. Ghahrem.-Nejad, Behcet & Demir sp. nova (Fabaceae), endemic to the eastern Anatolia, Turkey, is described here. It is placed in the sect. Incani due to its medifixed hairs, free stipules, bibracteolate flowers, and imparipinnate leaves, and it appears to be most closely related to A. siahcheshmehensis. Astragalus guzelsuensis differs, among other things, in having more leaflets (6-10 not 2-6 pairs), longer stipules (8-13 mm not 3-4 mm), a glabrous calyx (not hairy), a longer standard (29-31 not 25 mm), and a glabrous fruit (not hairy).
Comparative Karyological Analysis of Some Turkish Cuscuta L. (Convolvulaceae)
(Firenze Univ Press, 2022) Tasar, Neslihan; Tekbudak, Ilhan Kaya; Demir, Ibrahim; Acar, Mikail; Kursat, Murat
This study investigated the somatic chromosome numbers and morphomet-ric properties of 11 different taxa belonging to the genus Cuscuta L., which is one of the parasitic flowering plants and causes significant economic losses on agricultural products. For this purpose, the species were examined karyologically and compared statistically. Belonging to the genus Cuscuta, C. campestris Yunck., C. hyalina Roth, C. kotschyana Boiss., C. babylonica Aucher ex Choisy, C. europaea L., C. kurdica Engelm., C. brevistyla A.Braun ex A.Rich, C. planiflora Ten., C. approximata Bab., C. lupuliformis Krock. C. palaestina Boiss. the chromosome number and morphology of the species were investigated using karyological techniques. Chromosome numbers of the species; C. kotschyana, C. babylonica, C. europaea, C. kurdica, C.planiflora 2n=14; C. campes-tris, C. hyalina, C. approximata, C. lupuliformis, and C. palaestina 2n=28 and C. brevi-styla 2n=42 is determined. Also, the species' chromosome number, total chromosome length, relative length, arm ratio, centromere index and centromere states, and karyo-type asymmetry values were determined. Chromosome numbers of C. kotschyana and C. kurdica taxa were defined for the first time in this study. Thus, new data on the sys-tematics of these species have been revealed.
Effect of Priming Treatment on Germination at Stressful Temperatures, Fatty Acid, Sugar Content and Enzymatic Activity of Pepper Seeds
(Ankara Univ, Fac Agr, 2010) Kaya, Gamze; Demir, Ibrahim; Tekin, Aziz; Yasar, Fikret; Demir, Koeksal
This research was conducted to investigate the effects of priming (controlled hydration at 25 degrees C for 48 h) on germination at stressful temperatures (low 15 degrees C and high 35 degrees C), sugar content, total oil, fatty acid composition and enzymatic activities of pepper (Capsicum annuum L.) seeds in two consecutive runs. The experiments were conducted on pepper cultivars of Corbaci, Sera Demre 8 and Yalova Yaglik. Results revealed that priming enhanced germination of seeds of all cultivars at stressful temperatures. The highest increase in germination was determined in cv. Corbaci at (15 degrees C) with 12% and 35 degrees C with 21%. Priming decreased total oil content dependent upon cultivars. The highest decrease in oil content by priming was determined in Demre with 2.5-3.5%. On the other hand, fatty acid composition was not changed and the highest fatty acid of the seeds was linoleic acid (78.9%) followed by palmitic (9.16-11.79%) and oleic (7.28-11.40%). Priming resulted in decrease in sucrose which declined from 0.599% to 0.390% in Yalova Yaglik. Glucose was detected as trace in both control and primed seeds. The most important effects of priming on enzymatic activities were recorded in catalase which increased remarkably with priming in both runs. Catalase activity was measured in primed seeds with 11.2 mu mol min(-1) g(-1) and in control with 9.3 mu mol min(-1) g(-1) in run 1. They were determined as 16.6 mu mol min(-1) g(-1) and 9.6 mu mol min(-1) g(-1) in run 2, respectively. Even though not the same extent, priming also increased ascorbate peroxidase and superoxide dismutase activities.
Investigation of Palinological Features of Taxa Belonging To the Genus Cuscuta Distributed in Turkey
(Friends Science Publ, 2017) Demir, Ibrahim; Kaya, Ilhan; Benli, Mehlika; Altinoglu, Mustafa Kemal
In this study the species belonging to Cuscuta L. genus showing the distribution of agricultural and non-agricultural lands in Turkey was examined palynological perspective. This study was carried out between the years 2013-2015. The pollen morphology of 15 taxa of Cuscuta, including C. campestris Yunck., C. hyalina Roth., C. lupuliformis Krock., C. monogyna Vahl subsp. monogyna, C. monogyna Vahl subsp. esqumata (Englm.) Plitm., C. kotschyana Boiss. subsp. caudata Bornm & Schwarz, C. babylonica Aucher ex Choisy var. babylonica, C. babylonica Aucher ex Choisy var. elegans (Boiss. & Bal.) Englm., C. europaea L., C. kurdica Engelm., C. epithymum L., C. brevistyla A. Braun ex A. Rich., C. planiflora Ten., C. approximata Bab. var. approximata, and C. approximata Bab. var. macranthera (Boiss.) Feinbr. & Greuter was investigated by using scanning electron microscope (SEM). C. campestris and C. brevistyla species were found 3 (-4) zonocolpate, C. planiflora 3 (-6) zonocolpate. Pollen structure of taxa belonging to Cuscuta L. genus were determined prolate, subprolate, perprolate and prolate-spheroidal. The apertures of the pollens of these taxa were found scabrate, scabrate-perforate, oscabrate-perforate, reticulate and ekinate-reticulate. It was concluded that the pollen characteristics are convenient and a reliable method in identifying species. (C) 2017 Friends Science Publishers
Molecular Cloning and Sequence Analysis of the Its Region of Nuclear Ribosomal Dna for Species Identification in Dodders (Cuscuta; Convolvulaceae)
(Friends Science Publ, 2017) Keskin, Fatma; Kaya, Ilhan; Usta, Mustafa; Demir, Ibrahim; Sipahioglu, Hikmet Murat; Nemli, Yildiz
Dodder (Cuscuta sp.) is an obligate parasitic plant that is very difficult to control. In plants the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal DNA (nrDNA) has been considered one of the most important sequences for phylogenetic analysis. Here we report the analysis of nrDNA's ITS sequences as an efficient tool to study the phylogeny of dodders collected from various provinces of Eastern Anatolia (Turkey). Genomic DNA of six dodder samples belonging to 4 distinct species was extracted from body tissue samples. The sequences of 18S rRNA, ITS-1, 5.8S rRNA, ITS-2 and 26S rRNA regions of 4 Cuscuta species were determined by molecular cloning and sequencing. The identity of cloned fragments was compared to determine sequence identity using NCBI database. Bootstrap analysis of nrDNA of C. approximate, C. lupuliformis, C. campestris and C. babylonica indicated high sequence identity with similar sequences belonging to different geographical origins of the world retrieved from NCBI database. Our results clearly showed that the most stable secondary structure derived from the sequences obtained by universal ITS4 and ITS5 primers is very efficient tool for identification of Cuscuta species when used in combination with phylogenetic analysis. (C) 2017 Friends Science Publishers
Molecular Phylogeny Based on Its Sequences of Nrdna of Some Species Belonging To Dodder (Cuscuta L.) Genus From Various Ecological Sites of Turkey
(Univ Agr Sci & veterinary Med Cluj-napoca, 2020) Demir, Ibrahim; Kaya, Ilhan; Usta, Mustafa; Sipahioglu, Hikmet M.
Nuclear ribosomal DNA (nrDNA) sequence data of the Cuscuta genus, which have been considered as one of the most popular sequences for phylogenetic inference in plants, have been studied from a phylogenetic perspective in agricultural and non-agricultural lands of Turkey. The samples of Cuscuta spp. were collected from different geographical regions of Turkey between the years of 2013-2015. Some other species, not available locally, were taken from the herbarium samples of some research units. In order to study the phylogenetic relations of collected species, DNA isolations were made from body tissue samples. Conserved regions on ribosomal DNA (rDNA) were amplified by universal primers via PCR method and cloned into a proper cloning vector. The cloned DNA fragments were sequenced and analysed by web-based and computer programs. DNA sequences of certain species were recorded to the National Center for Biotechnology Information (NCBI) database. Based on the morphological examination and molecular analyses of fresh and the herbarium specimen, 8 species were identified. The identified species were C. hyalina (Gene bank accession no. KY020420), C. monogyna (KY020421), C. europaea (KY020422), C. palaestina (KY020423), C. approximata (KY020424), C. kurdica (KY020427), C. kotschyana (KY020430) and C. babylonica (KY020431). The ITS (Internal Transcribed Spacer) region contains several indels in identified Cuscuta species with the length varying from 668 to 730 bp. Sequence divergence ranges from 1.00% to 8.00% within Cuscuta spp. Based on our findings, the ITS sequences provided phylogenetically informative results in combination with the secondary structures.
Seed Morphology of Cuscuta L. (Convolvulaceae) in Turkey and Its Systematic Importance
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Demir, Ibrahim; Angishan, Firat; Tekbudak, Ilhan Kaya
Seed morphologies of 14 Cuscuta taxa from Turkey were examined by patterns of macro and micro morphological features were determined. Seed epidermis cell shapes were generally either irregular or polygonal. The surface pattern was mostly reticulate. This research revealed that Cuscuta species in Turkey have different seed morphologies. The results obtained will aid in the taxonomic evaluation of morphologically closely related species.