Browsing by Author "Demirel, A.F."

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Investigation of Genetic Polymorphisms of Csn1s1 and Blg Genes in Norduz Sheep by Pcr-Rflp Method
(Hellenic Veterinary Medical Society, 2023) Çak, B.; Yılmaz, O.; Demirel, A.F.
This study was undertaken to examine genetic polymorphisms of Alpha-Si casein (CSN1S1) and β-Lactoglobulin (BLG) genes in Norduz sheep by PCR-RFLP method. CSN1S1 and BLG genes have known a significant effect on cheese making. Restriction fragment length polymorphism (RFLP) is a molecular biology tool that reveals the difference between samples of homologous DNA molecules from differing locations of restriction enzyme sites.10 ml blood samples from 102 heads of Norduz sheep was used in the study.The genotypes of CSN1S1 and BLG genes was determination by PCR-RFLP method. Statistical analysis, after calculating allele and genotype frequencies by direct gene counting method, the distribution of observed and expected genotypic frequencies was determined according to the Hardy-Weinberg Equilibrium, and whether it was compatible with the x2 test.In the study conducted in Norduz sheep, polymorphism was observed in CSN1S1 and BLG genes. AA, AB and BB genotype frequencies of BLG gene were found to be 17.6%, 69.6% and 12.7%, respectively. It was determined that the BLG gene was not in Hardy-Weinberg Equilibrium. AA, AC and CC genotype frequencies of CSN1S1 gene were determined as 0.0%, 2.9% and 97.1%, respectively. The CSN1S1 gene was determined to be in Hardy-Weinberg equilibrium. In conclusion, it was determined that the A allele and AB genotype of the BLG gene were more common, the C allele and the CC genotype of the CSN1S1 gene were more common however, the AA genotype was not observed in Norduz sheep. © 2023 B Çak, O Yılmaz, AF Demirel. All Rights Reserved.
A Study on Milk Compositions of Hair Goat and Saanen X Hair Goat Crossbreed (F1) Under Semi-Intensive Conditions
(Ankara University, 2021) Çak, B.; Yilmaz, O.; Ocak, E.; Demirel, A.F.
This study was carried out to evaluate compositions of milk from Hair goat and Saanen x Hair goat crossbreed (F1) under semi-intensive conditions. The research was conducted on 20 Hair goats and 20 Saanen x Hair goat crossbreed (F1) at Farm for Research and Application of Van Yuzuncu Yil University in Turkey. All the experimental goats were 3 years old and were raised under semi-intensive conditions. In the current study, the average fat, non-fat solid, protein, lactose, total solids, lactic acid, pH values of Hair and crossbreed goat milks were 3.98%, 3.80%; 9.54%, 9.24%; 3.59%, 3.48%; 5.24%, 5.08%; 13.51%, 13.04%; 0.24%, 0.25%; 6.62, 6.64, respectively. Lactation stage had a significant influence (P<0.05; P<0.001) on milk fat, non-fat solid, protein, lactose, total solids contents and lactic acid value in Hair and crossbreed goats' milk. Also, genotype had a significant influence (P<0.001) on non-fat solid, protein, lactose and total solids contents of the milk. In conclusion, Hair goats and crossbreed goats produce milk with a variable milk fat, non-fat solid, protein, lactose, total solids contents at the different stages of lactation. The mean values of milk non-fat solid, protein, lactose and total solids in Hair goats' milk were higher than those of crossbreed goats. Also, milk fat content, lactic acid and pH values in Hair and crossbreed goats' milk were similar. © Ankara University, Faculty of Agriculture.