Browsing by Author "Demirer Durak, Emre"

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Antiviral and Antifungal Activity of Biologically Active Recombinant Bouganin Protein From Bougainvillea Spectabilis Willd
(Ankara Univ, Fac Agr, 2018) Guller, Abdullah; Siphioglu, Hikmet Murat; Usta, Mustafa; Demirer Durak, Emre
Bouganin antiviral protein (BAP) gene. one of the ribosome inactivating proteins, isolated from Bougainvillea speclabilis Willd. was cloned, expressed and the antiviral and antifungal activities were investigated. The full-length bouganin antiviral protein gene was amplified by reverse transcription-PCR using mRNA as template extracted from mature leaves. The coding region of bouganin gene was cloned into prokaryotic expression vector pETDuet-1 after amplification with end to end gene specific primers. The recombinant plasmid was transformed into Escherichia coli cells BL21(DE3)pLysS and the expression of BAP gene was induced by isopropyl beta-D thiogalactopyranoside (IPTG). Bouganin antiviral protein having a molecular mass of 28 kDa has been isolated from transformed bacterial colonies. Antiviral activity of bouganin was assayed against Zucchini yellow mosaic virus (ZYMV) by a mechanical inoculation test. The antifungal activity of purified recombinant protein was tested against pathogenic and non-pathogenic Rhizocionia solani. Trichoderma harzianum, and Fusarium arysporurn fungi using disc diffusion method. The increased amount of antiviral protein reduced the disease severity caused by ZYMV. The bouganin antiviral protein was inhibited the growth of R. solcati by 30.7% and of T harzianum by 20% after 72 h compared to control. No growth inhibition was observed for F oayporurn. All plants including controls treated with in vitro expressed BAP protein exhibited severe growth reduction compared with negative control (not treated) plants.
Biological Control of Rhizoctonia Solani on Potato by Using Indigenous Trichoderma Spp
(Amer inst Physics, 2016) Demirer Durak, Emre
At this study, it was aimed to determine the effect of Trichoderma isolates that was isolated from the soil samples taken from the different regions on black scurf and stem canker disease caused by Rhizoctonia solani Kuhn that has been one of the biggest problems of the potato cultivation. At the end of the soil isolations, totally 81 Trichoderma isolates were obtained and their species were identified. Of these isolates, T. harzianum (42%), T. virens (31%), T. asperellum (15%) and T. viride (12%). All of the isolates were tested in vitro for their antagonistic activity against the R. solani isolate. The isolates that show high inhibition rate was selected and tested against R. solani in vitro. Potato plants were grown in a greenhouse for about 10 weeks. Then the plants were evaluated according to the scale, plant height, shoot fresh and dry weights, root fresh and dry weights were noted. The experiment was conducted two times in three replications. At the in vitro tests, generally, it was determined that Trichoderma isolates have inhibited to R. solani and in vivo, they were reduced the effects of the disease and they were raised the development of the plant. In particular, it was determined that some isolates of the T. harzianum and T. virens have reduced the severity of the disease. It was determined that both in vitro and in vivo isolates have shown different efficiency against R. solani.
The Role of Arbuscular Mycorrhizal Fungi (AMF) in the Control of Rhizoctonia Root Rot in Local Bean Genotypes of the Van Lake Basin
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2025) Demirer Durak, Emre; Ekincialp, Aytekin; Gunes, Hasret; Erdinc, Ceknas
Beans (Phaseolus vulgaris L.), one of the main vegetables common in the Van Lake Basin, are frequently grown in the region with local genotypes. Rhizoctonia solani root rot, an important soil-borne disease that negatively affects beans, especially in this region, causes economic losses in our country and worldwide. Therefore, a viable and eco-friendly alternative to chemical control in the treatment of such significant soil-borne plant diseases is the application of Arbuscular Mycorrhizal Fungi (AMF). This study aimed to investigate the effects of commercial AMF (ERS) and Funneliformis mosseae (Fm) against R.solani (Rs) root rot disease in V29 and TR 50763 (T71) bean genotypes obtained from Van-Geva & scedil;. For this purpose, some plant growth parameters, total phenol, total antioxidant capacity, root colonization, and disease severity parameters were investigated. Fm and ERS significantly increased plant morphological parameters and reduced disease severity in both bean