Browsing by Author "Dogan, Abdulahad"

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Alterations in Histology and Antioxidant Defense System in the Testes of the Lake Van Fish (Alburnus Tarichi Guldenstadt, 1814)
(Springer, 2016) Kaptaner, Burak; Kankaya, Ertugrul; Dogan, Abdulahad; Durmus, Atilla
Recent reports have demonstrated gonadal abnormalities in the Lake Van fish (Alburnus tarichi) from Lake Van caused by increasing pollution. In the present study, the fish was collected from an area of Lake Van receiving mainly sewage treatment plant effluent at prespawning period (April) and from a river (Karasu) which is close to the polluted area of the lake and where the fish migrates at spawning period (May). Collected specimens were examined for testicular alterations, gonadosomatic index (GSI), condition factor (CF), and antioxidant defense system biomarkers based on comparison with a reference lake (Ercek) and a reference freshwater inlet (Memedik River). Histological examinations of the testes of fish from the polluted area and the connected river showed various alterations consisting of macrophage aggregates, vacuolation, pyknosis, germ cell degeneration, seminiferous tubule dilation, disorganization of tubules, reduced spermatozoa, and fibrosis. A lower GSI and CF were also observed. Moreover, alterations in the antioxidant system biomarkers were determined in the testis tissues of fish from the Lake Van and Karasu River, indicating oxidative stress. These results suggest that the abnormalities in the testes are causally related to the increased oxidative stress, and pollution in Lake Van may have adversely affected the reproductive health of the lake Van fish.
Ameliorative Effects of Scutellaria Pinnatifida Subsp. Pichleri (Stapf) Rech.f. Extract in Streptozotocin-Induced Diabetic Rats: Chemical Composition, Biochemical and Histopathological Evaluation
(Bmc, 2023) Bakac, Mehmet Salih; Dogan, Abdulahad; Yilmaz, Mustafa Abdullah; Altindag, Fikret; Donmez, Fatih; Battal, Abdulhamit
BackgroundsScutellaria Pinnatifida subsp. pichleri (Stapf) Rech.f. (SP) is used in folk medicine for the treatment of diabetes. The aim of the study was to determine the phenolic profile of SP extract (SPE) by LC-MS/MS and to investigate the antidiabetic, hepatoprotective and nephroprotective effects of SPE in streptozotosin (STZ)-induced diabetic rat model.MethodsForty-two rats were randomly divided into six groups (n = 7): Control (nondiabetic), diabetes mellitus (DM), DM + SP-100 (diabetic rats treated with SPE, 100 mg/kg/day), DM + SP-200 (diabetic rats treated with SPE, 200 mg/kg/day), DM + SP-400 (diabetic rats treated with SPE, 400 mg/kg/day) and DM + Gly-3 (diabetic rats treated with glibenclamide, 3 mg/kg/day). Live body weight, fasting blood glucose (FBG) level, antidiabetic, serum biochemical and lipid profile parameters, antioxidant defense system, malondyaldehyde (MDA) and histopathological examinations in liver, kidney and pancreas were evaluated.ResultsApigenin, luteolin, quinic acid, cosmosiin and epigallocatechin were determined to be the major phenolic compounds in the SPE. Administration of the highest dose of SP extract (400 mg/kg) resulted in a significant reduction in FBG levels and glycosylated hemoglobin levels in STZ-induced diabetic rats, indicating an antihyperglycemic effect. SPE (200 and 400 mg/kg) and glibenclamide significantly improved MDA in liver and kidney tissues. In addition, SPE contributed to the struggle against STZ-induced oxidative stress by stimulating antioxidant defense systems. STZ induction negatively affected liver, kidney and pancreas tissues according to histopathological findings. Treatment with 400 mg/kg and glibenclamide attenuated these negative effects.ConclusionsIn conclusion, the extract of the aerial part of Scutellaria pinnatifida subsp. pichleri has hepatoprotective, nephroprotective and insulin secretion stimulating effects against STZ-induced diabetes and its complications due to its antidiabetic and antioxidant phytochemicals such as apigenin, luteolin, quinic acid, cosmosiin and epigallocatechin.
Ameliorative Role of Cyanus Depressus (M.bieb.) Sojak Plant Extract Against Diabetes-Associated Oxidative-Stress Liver, Kidney, and Pancreas Damage in Rats
(Wiley, 2022) Duman, Kendal Erdem; Dogan, Abdulahad; Kaptaner, Burak
In this original article, we aimed to assess the ameliorative role of Cyanus depressus (CD) plant ethanolic extract treatment of streptozotocin (STZ)-induced liver, kidney, and pancreas damage in rats. The rats were divided into five groups (n = 7): control, CD, Diabetes mellitus (DM), DM + CD, and DM + glibenclamide (Gly). The DM groups were injected with a single dose of 50 mg/kg STZ intraperitoneally (i.p.). While the CD and DM + CD groups received 400 mg/kg/day intragastrically for 21 days, the DM + Gly group received 3 mg/kg/day of Gly intragastrically throughout the experiment. Statistically significance was accepted as p < .05. According to our liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) data, quinic acid, cosmosiin, nicotiflorin, apigenin, and protocatechuic acid were the major compounds, in descending order. Weekly blood glucose, serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and urea, malondialdehyde (MDA) (liver and pancreas), and blood glycosylated hemoglobin % (HbA1c %) were significantly decreased, whereas finally live body weights (LBWs), reduced glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) (pancreas), and pancreatic islet diameter and area were increased significantly in the CD-treated diabetic group. Moreover, CD administration was found to be effective in the protection of the histology of the liver, kidneys, and pancreatic islets in the STZ-induced rats. Consequently, we concluded that CD administration reduces hyperglycemia, oxidative stress, and histopathology in STZ-induced experimental rats by improving antioxidant defenses. Practical applications Today, the prevalence of diabetes is increasing rapidly throughout the world and it causes complications such as kidney damage, blindness, amputations, and cardiovascular diseases. Despite medical technological advances, people's interest in medicinal herbal products is gradually increasing. Biochemical and histopathological findings showed that the use of the plant CD at the determined dose (400 mg/kg/day) in rats with DM by STZ had strong antioxidant and antidiabetic effects. CD may have a drug potential in preventing DM and its complications because of its phytochemical content including some phenolic acids such as quinic acid, cosmosiin, nicotiflorin, apigenin, and protocatechuic acid. Isolation of bioactive compounds from CD and investigation of their therapeutic effects could be planned as further studies.
Anormal Gonadlı Van Balığının (Alburnus Tarichi Güldenstädt, 1814) Bazı Dokularında Oksidatif Hasarın Belirlenmesi
(2017) Ergöz, Burcu; Oğuz, Ahmet R; Özok, Necati; Dogan, Abdulahad
Van Gölü dünyanın en büyük sodalı gölü olup gölde ekstrem şartlara uyum sağlayan tek bir balık türü olan Van balığı yaşamaktadır. Son yıllarda, gölün kirliliğine ilişkin pek çok çalışma yapılmıştır. Özellikle balıklar arasında gözlenen gonad anormallikleri bunlardan en önemlisidir. Bu çalışmada, Van gölünde ovaryum anormalliği belirlenen Van balığı ve normal balıklarda malondialdehit (MDA) seviyeleri ve redükte glutatyon (GSH) düzeyleri ile superoksid dismutaz (SOD) ve glutatyon peroksidaz (GSH-px) gibi antioksidan enzim seviyeleri solungaç, karaciğer ve gonadlarda karşılaştırıldı. MDA seviyeleri anormal bireylerde normal bireylere göre bütün dokularda önemli oranda artış gösterdi (P<0.05). Anormal bireylerin solungaç ve ovaryum dokularındaki SOD aktivitesi ve karaciğer dokusundaki GSH-Px aktivitesi normal bireylere göre azalma önemli bulundu (P<0.05). GSH seviyesinde ise normal ve anormal dokuların karşılaştırılmasında fark bulunmadı. Sonuç olarak belirli bir dönemde kirleticilere maruz kaldığı düşünülen anormal ovaryumlu balıkların normal balıklarla karşılaştırıldığında antioksidant savunma sistemlerinin etkilendiği söyleyenebilir
Antidiabetic Properties of Lyophilized Extract of Acorn (Quercus Brantii Lindl.) on Experimentally Stz-Induced Diabetic Rats
(Elsevier Ireland Ltd, 2015) Dogan, Abdulahad; Celik, Ismail; Kaya, Mehmet Salih
Ethnopharmacological relevance: Acorn obtained from the Quercus brantii Lindl. (QB) tree has been used in Turkish folk medicine. Some studies have reported as an antioxidant, antimicrobial, anti-inflammatory, gastroprotective, antitumoural of QB properties previously, however, its effect on the management of type diabetic 2 and oxidative stress complications is still unexplored. The aims of our study were the evaluation of the protective effect and antioxidant role of acorn lyophilized seed plant extract against STZ-induced diabetic complications as oxidative stress, hepatotoxicity and nephropathy, lipidemia and serum biomarkers of diabetes (SBD). Materials and methods: In-vivo studies were performed on STZ-induced diabetic rats Experiment was designed as I [Normal Control (NC)], II [Diabetes mellitus (DM)], III [DM+Acarbose (20 mg/kg b.w) (DM+AC-20)], IV [DM+ QB (100 mg/kg b.w) (DM+QB-100)], V [DM+QB (250 mg/kg b.w) (DM+ QB-250)] and VI [DM+QB (500 mg/kg b.w) (DM+QB-500)] groups. Results: This study showed that the biochemical analysis showed a considerable increase in the HRDB, DB, LP, MDA and fluctuated ADSC in the II group as compared to that of control group whereas, AC and the plant lyophilized seed plant extract supplementations diet restored the STZ-induced diabetic complications towards the control. alpha-glycosidase activity in DM group showed statistically significant increase with respect to control group in small intestine. Moreover, in accordance with the effects of seed extract; in diabetic rat groups to whom acorn seed extract and acarbose were given, the levels of almost all the concerned parameters were reached to the ones measured at control group. As a result, it was concluded that acorn seed extract had certain healing effects on many complications caused by diabetes. (c) 2015 Elsevier Ireland Ltd. All rights reserved.
Bisfenol F’nin Sıçan Pankreası Üzerindeki Toksik Etkileri
(2022) Dogan, Abdulahad; Doğan, Emine; Kaptaner, Burak
Bu çalışmada, bisfenol F (BPF)’nin sıçan pankreası üzerindeki toksik etkilerinin belirlenmesi amaçlandı. Bu amaç doğrultusunda yirmi sekiz adet Wistar albino erkek sıçan, BPF’ye 0, 20, 100 ve 500 mg/kg vücut ağırlığı (v.a.) konsantrasyonlarda, 28 gün boyunca oral gavaj ile maruz bırakıldı. Histolojik incelemelere göre BPF maruziyeti sonrasında, pankreatik Langerhans adacıklarında vakuolar dejenerasyon gösteren hücrelerin varlığı tespit edildi. Histomorfolojik ölçümler, BPF’nin 100 ve 500 mg/kg v.a. konsantrasyonlarına maruz bırakılan gruplarda, adacık çap ve alan ortalamalarının düştüğünü gösterdi. Ek olarak, adacıklarda immunohistokimyasal olarak boyayan insülin pozitif  hücre yüzdesinin, BPF uygulanan bütün gruplarda, anlamlı bir şekilde azaldığı tespit edildi. Serum açlık glukoz, total kan HbA1c ve serum C-peptid ile insülin seviyelerinin, BPF maruziyeti sonrasında anlamlı değişimler göstermedikleri gözlendi. BPF’nin malondialdehit düzeyi dışında, pankreas antioksidan savunma sistemi belirteçlerinde anlamlı değişimlere yol açtığı belirlendi. Elde edilen sonuçlar BPF’nin endüstriyel alanlarda, bisfenol A yerine güvenilir bir alternatif olarak, kullanımına daha fazla dikkat edilmesi gerektiğini göstermektedir.
Ccı4 İle Oksidatif Stres Oluşturulan Sıçanlarda Pholiota Aurivella Liyofilize Ekstrenin Bazı Biyokimyasal ve Hematolojik Parametrelere Etkisi
(2018) Dogan, Abdulahad
Mantarlar, organizmaları bazı zararlı bileşiklerin toksik etkilerinden korumak için önemli işlev görürler. Bu çalışmada, karbon tetraklorür (CCl4) ile oksidatif stres oluşturulan sıçanlarda P.aurivella liyofilize ekstrenin kan parametreleri üzerindeki etkilerinin değerlendirilmesi amaçlanmıştır. Ratlar, toksisite testi sonrası dört deney grubuna ayrıldı; Kontrol, CCI4, CCI4+P.aurivella (100 mg/kg ekstre) ve CCI4+P.aurivella (500 mg/kg ekstre) grupları. 4 haftalık muamele sonunda, CCI4’un oksidatif stresine karşı P.aurivella ekstraktının oral alımının hematolojik parametrelere, eritrosit farjilitesine ve sıçanların eritrositlerinde antioksidan savunma unsurlarından redükte glutatyon (GSH), glutatyon redüktaz (GR), glutatyon S-transferaz (GST), katalaz (CAT), glutatyon peroksidaz (GPx), süperoksid dismutaz (SOD) aktiviteleri ve malondialdehit (MDA) içeriği tespit edilerek değerlendirilmiştir. Sonuçlara göre, CCI4 ve CCI4+P.aurivella ekstresi uygulanan gruplarda GSH düzeyi Kontrol grubuna göre önemli düşüş gösterdi. Diğer yandan, CCI4 grubundaki GPx aktivitesi Kontrol ve CCI4+P.aurivella gruplarına göre önemli düşüş gösterdi. Ayrıca, CCI4 ve CCI4+P.aurivella gruplarında eritrosit frajilitesi (özellikle % 0.6 NaCl konsantrasyonunda) Kontrol grubuna göre önemli artış gözlendi. CCI4 grubunda RBC, HGB, HCT, WBC, PLT and PCT gibi bazı hematolojik parametrelerde önemli azalma gösterirken RDWs de ise önemli artma belirlendi. Bakılan parametreler üzerinde CCI4’ün zararlı etkileri olmasına rağmen bunu P.aurivella için söylemek zordur. Fakat, P.aurivella eritrosit hemolizine katkı sunduğu için zararlı olabilir. Bu yüzden insanların bu mantarı tüketiminde dikkatli davranması gerektiği sonucuna varılmıştır.
Consumption of the Syrian Mesquite Plant (Prosopis Farcta) Fruit and Seed Lyophilized Extracts May Have Both Protective and Toxic Effects in Stz-Induced Diabetic Rats
(Taylor & Francis Ltd, 2022) Agirman, Esvet; Celik, Ismail; Dogan, Abdulahad
The aim of study was to investigate the antidiabetic and antioxidant properties of extracts obtained from dried Prosopis farcta fruit and seeds against streptozotocin-induced diabetes in rats. According to the results, glucose, haemoglobin A1c, alpha-glycosidase activity, liver and kidney damage biomarkers, and malondialdehyde contents of all of the diabetic groups were found to have increased significantly according to the control group. Furthermore, the insulin and C-peptide secretions increased, and liver malondialdehyde level decreased, which were determined as the result of fluctuations in the antioxidant enzyme activities with a dose of 400 mg/kg fruit extract, while seed extract dosages of 100 and 400 mg/kg caused an increase in hepatic demage biomarkers. It was concluded that fruit extract may have insulin secretion stimulating and lipid peroxidation inhibitory effects, whereas seed extract might have caused hepatocyte damage changes to the transport functions and membrane permeability of these cells, thus causing enzymes to leak.
Determination of Hepatoprotective and Antioxidant Role of Walnuts Against Ethanol-Induced Oxidative Stress in Rats
(Humana Press inc, 2015) Bati, Bedia; Celik, Ismail; Dogan, Abdulahad
The aims of our study were the evaluation of the hepatoprotective effect and antioxidant role of walnuts against ethanol-induced oxidative stress. The hepatoprotective and antioxidant role of the walnuts supplementation feed against ethanol-induced oxidative stress were evaluated by measuring liver damage serum marker enzymes; aspartate aminotransferase (AST); alanine aminotransferase (ALT); gamma glutamyl transpeptidase (GGT); lactate dehydrogenase (LDH); and antioxidant defense systems such as reduced glutathione, glutathione reductase, superoxide dismutase, glutathione-S-transferase, catalase, and glutathione peroxidase and Malondialdehyde (MDA) content in various tissues of rats. Rats were divided into six experimental groups: I (control), II (20 % ethanol), III (10 % walnuts), IV (20 % ethanol + 10 % walnuts), V (5 % walnuts), and VI (20 % ethanol + 5 % walnuts). According to the results, the biochemical analysis showed a considerable increase in the serum aspartate AST, ALT, GGT, and LDH in the group II as compared to that of group I, whereas decreased in group IVas compared to that of group II. In addition, administration of walnuts supplementation restored the ethanol-induced imbalance between MDA and fluctuated antioxidant system toward close control group particularly in the tissues. The results indicated that walnuts could be as important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the lipid oxidation or inhibiting the production of ethanol-induced free radicals in rats.
Determination of Tissue Protective and Antioxidant Activities of Two Medicinal Mushrooms From Turkey Against Ccl4-Lnduced Experimental Oxidative Stress in Rats
(Begell House inc, 2020) Hasar, Songul; Dogan, Abdulahad; Demirel, Kenan
This study aimed at determining hepatoprotective and antioxidants effects of Lactarius deliciosus and Agrocybe cylindracea against CCl4-induced oxidative stress (OS) in rats. Herein, 36 rats were divided into 6 groups: (I) Control, (II) CCl4, (III) CCl4 + L. deliciosus, (IV) L. deliciosus, (V) CCl4 + A. cylindracea, and (VI) A. cylindracea. According to the 7-week study results, aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione S-transferase (GST) (brain), superoxide dismutase (SOD) (kidney), malondialdehyde (MDA) (kidney), MDA (spleen), MDA (erythrocyte), GST (erythrocyte), and GST (liver) in group III (at 10%, 26%, 34%, 57%, 7%, 25%, 42%, 27%, and 35%, respectively) were significantly lower than in group II. Moreover, the reduced glutathione (GSH) (spleen) and catalase (spleen) in group III (at 20% and 38%, respectively) were significantly higher than in group II. AST, ALT, tri-glyceride, glutathione reductase (GR) (brain), GR (kidney), SOD (spleen), and SOD (liver) in group V (at 194%, 147%, 38%, 36%, 66%, 8%, and 7%, respectively) were significantly higher than in group II. However, MDA (kidney), MDA (erythrocyte), (GSH) (erythrocyte), MDA (liver), GSH (liver), and GST (liver) in group V (at 48%, 35%, 34%, 15%, 30%, and 41%, respectively) were significantly lower than in group II. Hence, while L. deliciosus may exhibit tissue-pro-tective against CCl4-induced OS in rats, A. cylindracea does not.
Editorial: Model Organisms in Predictive Toxicology 2023
(Frontiers Media Sa, 2025) Luceri, Cristina; Dogan, Abdulahad; Leon-Buitimea, Angel
Evaluation of the Erythrocyte Fragility, Haematological Parameters and Antioxidant Properties of Platanus Orientalis Leaf Infusion Against Ethanol Toxicity in Rats
(Adem Yavuz Sonmez, 2020) Dogan, Abdulahad; Donmez, Fatih; Anuk, Ozgur Ozan
The aim of this study was to evaluate the protective effects of the leaf infusion of chinar (Platanus orientalis L.) on erythrocyte fragility, haematological parameters and antioxidant status against ethanol-induced oxidative stress in rats. Thirty male rats were divided into five groups: Control, Ethanol, Ethanol+Silymarin (10 mg kg(-1)), Ethanol+PO-20 mg mL(-1) infusion, and Ethanol+PO-60 mg mL(-1) infusion. According to the results, in the Ethanol group, erythrocyte counts, red cells distribution, plateletcrit, platelet and lymphocyte levels significantly decreased compared to the Control group, while PO-60 dose-fed group showed a significant increase in haematocrit and haemoglobin values compared to the Ethanol group. There were significant changes in erythrocyte fragility of Ethanol and Ethanol-treatment groups at different NaCl concentrations of 0.3 and 0.6 according to Control group. It was observed that PO Leaf infusion reduced the hemolysis caused by ethanol at a concentration of 0.3% NaCl, thus reducing the values to the control values. In addition, PO leaf infusion caused a significant increase in total antioxidant status against ethanol toxicity and a significant decrease in total oxidative status and oxidative stress index. It was concluded that PO leaf infusion may have antihematotoxic effect, reducing erythrocyte fragility and increase antioxidant capacity against ethanol toxicity.
Evaluation of the in Vitro and in Vivo Effects of the Ethanolic Lyophilized Extract Obtained From Pholiota Aurivella Mushroom on Cci4-Induced Toxicity in Rats
(Parlar Scientific Publications (p S P), 2019) Dogan, Abdulahad; Dalar, Abdullah; Battal, Abdulhamit; Uyar, Ahmet; Sadullahoglu, Cemil; Celik, Ismail; Uzun, Yusuf
The aim of this study was to evaluate Pholiota aurivella extract's chemical and mineral composition, total antioxidant capacity, histopathological changes in liver and kidney, as well as its possible protective/harmful role against carbon tetrachloride (CCl4)-induced oxidative stress in rats. After the toxicity test, twenty four rats were divided into four experimental groups: Control, CCl4, CCl4+P. aurivella (100 mg/kg, extract) and CCl4+P.aurivella (500 mg/kg, extract) groups. Some biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) in serum, malondialdehyde (MDA) and antioxidant defense system (ADS) constituents such as reduced glutathione (GSH) levels, glutathione reductase (GR), glutathione S-transferase (GST), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activities in the liver, brain and kidney tissues, histopathological changes in liver and kidney of rats were observed. P.aurivella extract was rich in phenolic (p-coumaric and protocatechuic acids) and fatty acid (linoleic, oleic and palmitic acids) compounds and high level of Arsenic. In vivo studies revealed that P.aurivella extract suppressed the levels of ADS enzymes and increased the MDA content and AST, ALT and LDH in treated groups. According to histopathological investigation, liver and kidney revealed severe histopathological changes both in CCl4 group and P.aurivella- treated groups. These results have shown that oral administration of P. aurivella extract caused a significant toxic effects in rats. Therefore, in the context of the data obtained from this study the P. aurivella could not be regarded as a safe food source.
Exploring of the Ameliorative Effects of Nerium (Nerium Oleander L.) Ethanolic Flower Extract in Streptozotocin Induced Diabetic Rats Via Biochemical, Histological and Molecular Aspects
(Springer, 2023) Battal, Abdulhamit; Dogan, Abdulahad; Uyar, Ahmet; Demir, Abdulbaki; Keles, oemer Faruk; Celik, Ismail; Aslan, Ali
Background Nerium oleander L. is ethnopharmacologically used for diabetes. Our aim was to investigate the ameliorative effects of ethanolic Nerium flower extract (NFE) in STZ-induced diabetic rats.Methods Seven random groups including control group, NFE group (50 mg/kg), diabetic group, glibenclamide group and NFE treated groups (25 mg/kg, 75 mg/kg, and 225 mg/kg) were composed of forty-nine rats. Blood glucose level, glycated hemoglobin (HbA1c), insulin level, liver damage parameters and lipid profile parameters were investigated. Antioxidant defense system enzyme activities and reduced glutathione (GSH) and malondialdehyde (MDA) contents and immunotoxic and neurotoxic parameters were determined in liver tissue. Additionally, the ameliorative effects of NFE were histopathologically examined in liver. mRNA levels of SLC2A2 gene encoding glucose transporter 2 protein were measured by quantitative real time PCR.Results NFE caused decrease in glucose level and HbA1c and increase in insulin and C-peptide levels. Additionally, NFE improved liver damage biomarkers and lipid profile parameters in serum. Moreover, lipid peroxidation was prevented and antioxidant enzyme activities in liver were regulated by NFE treatment. Furthermore, anti-immunotoxic and anti-neurotoxic effects of NFE were determined in liver tissue of diabetic rats. Histopathogically, significant liver damages were observed in the diabetic rats. Histopathological changes were decreased partially in the 225 mg/kg NFE treated group. SLC2A2 gene expression in liver of diabetic rats significantly reduced compared to healthy rats and NFE treatment (25 mg/kg) caused increase in gene expression.Conclusion Flower extract of Nerium plant may have an antidiabetic potential due to its high phytochemical content. [Graphics] .
Exploring Phytochemical Constituents of Achillea Arabica Kotschy. Ethanolic Flower Extract by Lc-ms/Ms and Its Possible Antioxidant and Antidiabetic Effects in Diabetic Rats
(Walter de Gruyter Gmbh, 2023) Hanalp, Hanife Ceren; Dogan, Abdulahad; Saygi, Tuba Kusman; Donmez, Fatih; Battal, Abdulhamit
The aim of this study was to reveal the antidiabetic and antioxidant effects of ethanolic lyophilized extract of Achillea arabica flower extract against streptozotosine (STZ)-induced in diabetic rats and to determine its phytochemical content by liquid chromatography with tandem mass spectrometry (LC-MS/MS). After toxicity test, 35 female rats were divided into five groups. Control, diabetes mellitus (DM), A.arabica (400 mg/kg) extract, DM + A. arabica (400 mg/kg) extract and DM + Glibenclamide (2 mg/kg). It was determined that while diabetic rats treated A.arabica plant extract significantly decreased blood glucose level, serum glucose, HbA1c, liver and kidney damage biomarker levels, and malondialdehyde (MDA) content compared to the DM group, it caused fluctuations in antioxidant enzyme levels. According to LC-MS/MS results of A. arabica flower extract, quinic acid (2439.9 mu g/g), cyranoside (858.4 mu g/g), chlorogenic acid (698.7 mu g/g), and cosmosiin (347.8 mu g/g) were determined as major compounds, respectively. In addition, two new compounds were determined in this extract according to nuclear magnetic resonance (NMR) and Mass analyses and these compounds were named edremitine and achillosine, respectively. Thus, A.arabica flower extract has possible therapeutic effects to prevent high blood glucose level and oxidative stress caused by DM in liver and kidney via its high phenolic content.
Exploring the Ameliorative Effects of Hypericum Scabrum L. on a Surgically-Induced Endometriosis Rat Model and Its Phytochemical Lc-ms/Ms- Profile
(Soc Stiinte Farmaceutice Romania, 2023) Ilhan, Mert; Battal, Abdulhamit; Kaptaner, Burak; Dogan, Abdulahad; Donmez, Fatih; Yilmaz, Mustafa abdullah; Eroglu, Huseyin
Endometriosis is described as the existence and expansion of functional endometrial-like tissues in another part of the uterine cavity. It affects not only postmenopausal women but also teenagers after menarche, causing subfertility, dysmenorrhea and severe pelvic pain. Since no specific treatment is acknowledged for this disease, the present study aimed to investigate the efficacy of H. scabrum in a surgically-induced endometriosis rat model. In order to test the efficacy of H. scabrum on endometriosis, n-hexane, ethyl acetate (EtOAc), methanol (MeOH) and aqueous extracts were prepared from the aerial parts of H. scabrum. The cytokine levels, antioxidant enzyme activities, the contents of malondialdehyde (MDA) and glutathione (GSH) and histopathological analyses were evaluated. In order to reveal the potential active compounds, liquid chromatography-mass/mass spectroscopy (LC-MS/MS) analysis was performed on the MeOH extract who presentedthe highest activity. The MeOH extract significantly decreased the adhesion scores, endometriotic implant volumes, cytokine levels and the antioxidant parameters. Furthermore, histological procedures supported those findings. According to LC-MS/MS analysis of the MeOH extract, the major compounds were mostly phenolic acids and flavonoids. We concluded that the MeOH extract from H. scabrum aerial parts could be used for the treatment of endometriosis, and its activity could be attributed to the chlorogenic acid and quercetin, detected in the highest amounts.
Healing Effects of Sumac (Rhus Coriaria) in Streptozotocin-Induced Diabetic Rats
(Taylor & Francis Ltd, 2016) Dogan, Abdulahad; Celik, Ismail
Context Sumac [Rhus coriaria L. (RC) (Anacardiaceae)] is used as a folk medicine in the treatment of diabetes in Turkey. Objective This study investigates the in vivo healing and protective effects of lyophilized extract sumac against streptozotocin (STZ)-induced diabetic complications. Materials and methods Toxicity test was conducted in three different dosages (250, 500 and 1000 mg/kg of plant extracts, respectively). Six groups of seven rats each were used in experiments. Groups were designed as Normal control, Diabetic (DM), DM + AC-20 mg/kg, DM + Extract-100 mg/ kg, DM + Extract 250 mg/kg and DM + Extract 500 mg/kg group. Experimental diabetes [50 mg/kg, intraperitoneal (i.p.)] was induced by STZ. The effects of oral administration of the extract for 21 d on the level of serum glucose, insulin, C-peptide, lipid profile (LP), hepatic and renal damage biomarkers (HRDB), diabetic serum biomarkers (DSB), glycosylated haemoglobin (HbA1c), antioxidant defence system constituents (ADSCs), malondialdehyde (MDA) and alpha-glucosidase activity in small intestine tissue were evaluated. Results The extract decreased the levels of blood glucose in diabetic groups (an average of 31%). Triglyceride, total cholesterol, high-density lipoprotein and low-density lipoprotein levels were balanced by plant extract (500 mg/kg) supplementation in the diabetic group. Decreased levels of aspartate aminotransferase (89%), alanine aminotransferase (91%), lactate dehydrogenase (35%), alkaline phosphatase (47%), creatinine (25%) and urea (29%) were detected in plant extract (500 mg/kg) supplemented diabetic group. Additionally, a considerable increase in the HRDB, DSB, LP, MDA and fluctuated ADSC levels were restored in RC-extract supplemented groups. Conclusion RC lyophilized extract has a healing effect on diabetes and diabetes-related complications.
Hepatoprotective and Antioxidant Activities of Grapeseeds Against Ethanol-Induced Oxidative Stress in Rats
(Cambridge Univ Press, 2012) Dogan, Abdulahad; Celik, Ismail
The present study was carried out to evaluate the hepatoprotective effect and antioxidant role of grape (Vitis vinifera L.) seeds (GS) against ethanol-induced oxidative stress. The hepatoprotective and antioxidant roles of the GS supplementation feed against ethanol-induced oxidative stress were evaluated by measuring liver damage serum marker enzymes, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transpeptidase and lactate dehydrogenase, antioxidant defence system such as GSH, glutathione reductase, superoxide dismutase, glutathione S-transferase and glutathione peroxidase and malondialdehyde (MDA) content in various tissues of rats. Rats were divided into four experimental groups: I (control), 11 (20% ethanol), III (15% GS) and IV (20% ethanol + 15% GS). According to the results, the level of serum marker enzymes was significantly increased in group II as compared to that of group I, but decreased in group IV as compared to that of group II. Also, administration of GS-supplemented food restored the ethanol-induced MDA, which was increased near the control level. The results indicated that GS could be as important as diet-derived antioxidants in preventing oxidative damage in the tissues by reducing the lipid oxidation or inhibiting the production of ethanol-induced free radicals in rats.
Histochemical Changes, Cellular Dynamics and Antioxidant Responses in the Corpuscles of Stannius of the Female Lake Van Fish (Alburnus Tarichi Guldenstadt, 1814) (Cyprinidae) During Migration From Highly Alkaline and Brackish Water To Freshwater
(Wiley, 2022) Kaptaner, Burak; Dogan, Abdulahad; Aykut, Handan; Yildiz, Fatos; Donmez, Fatih; Dogan, Emine
The Lake Van fish (Alburnus tarichi) is an anadromous species, which is endemic to Lake Van Basin of Turkey. In spring, the fish migrates from Lake Van, which has highly alkaline and brackish water, to freshwater to spawn. During the anadromous migration of the female Lake Van fish, histochemical changes in the cell types, cellular dynamics, apoptosis, cell proliferation and antioxidant responses in the corpuscles of Stannius (CS) which are tiny endocrine glands related with calcium homeostasis were examined. When compared to the lake, calcium concentrations of ambient water and plasma increased considerably in freshwater. Freshwater dramatically increased the number of predominant type-I cells in the gland while decreased the type-II cells. A reduction in the cytoplasmic periodic acid-Schiff content of type-I cells was observed in freshwater. No cytoplasmic staining was found in cytoplasm of type-II cells in both lake and freshwater. Apoptotic cells were detected in fish sampled from both the lake and freshwater and a nonsignificant increase was observed in freshwater. The number of proliferating cells increased significantly in the freshwater, as well as antioxidant defences such as catalase, glutathione peroxidase and glutathione s-transferase activities, which indicates an elevated oxidative status. The findings suggest that type-I cells are possible source of a hypocalcin hormone and type-II cells are considered to be a likely precursor of type-I cells. Apoptosis and cell proliferation are basic mechanisms of gland remodelling. In addition antioxidant mechanisms play a role during the acclimation of the gland in different environments.
Investigation of Neurotoxic and Immunotoxic Effects of the Chinar (Platanus Orientalis L.) Tree Leaf Infusion Against Ethanol Toxicity in Rats
(2020) Donmez, Fatih; Battal, Abdulhamit; Dogan, Abdulahad; Rae, Iain Jonathan; Anuk, Özgür Ozan
In this study, immunotoxic and neurotoxic effects of leaf infusion of Platanus orientalis L. (PO) were investigated in rats ethanol-induced toxicity. Neurotoxic effects of ethanol were evaluated by measuring acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in rat brain tissue. Immunotoxic effects were evaluated by adenosine deaminase (ADA) and myeloperoxidase (MPO) biomarkers in rat liver, lung, erythrocyte and spleen tissues. Thirty male Wistar rats were divided into five groups after toxicity study. The groups are explained as follow: Control, Ethanol 20 %, Ethanol 20 % + Silymarin (10 mg kg-1), Ethanol 20 % + PO-20 mg mL-1 infusion and Ethanol 20 % + PO-60 mg mL-1 infusion. The results showed that AChE and BChE activities statistically decreased in the groups treated with PO leaf infusion. While there was a statistically significant decrease in ADA and MPO activities in liver tissue of groups treated with PO leaf infusion. Also, MPO activity of erythrocyte in ethanol group was significantlly increased according to silymarin and PO-60 groups. On the other hand, there was no statistically significant finding was found in lung and spleen tissues. It was concluded that the infusion prepared from P. orientalis leaves suppressed to ethanol-induced neurotoxicity and immunotoxicity.