Browsing by Author "Donmez, F."

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Exploring the Protective Effects of Chinar (Platanus Orientalis L.) Leaf on Testes Damage Induced by Ethanol in Rat Model
(Pakistan Agricultural Scientists Forum, 2024) Yaman, T.; Dogan, A.; Keles, O. F.; Donmez, F.; Battal, A.; Anuk, O. O.
The aim of this study was to investigate the protective effects of an infusion obtained from dried Chinar (Platanus orientalis L.; PO) leaves against ethanol (EtOH)-induced damage in rat testes. Thirty male Wistar rats were randomly and equally divided into 5 groups, as the Control, EtOH 20%, EtOH 20% + Silymarin (10 mg/kg/day), EtOH 20% + PO20 mg/mL infusion, and EtOH 20% + PO -60 mg/mL infusion. Histopathologically, treatment with PO -60 leaf infusion resulted in improvements in EtOH-induced damage in the testes by inhibiting the depletion of germ cells and loss of spermatozoa. Immunohistochemically, an increase in the expression of endothelial nitric oxide synthase (eNOS) and caspase-3 was observed in the EtOH group. Treatment with the PO extract markedly reduced the EtOH-induced expression of eNOS and caspase-3 in the EtOH 20% + PO -60 group. According to the biochemical results, both of the PO infusion treatments caused a decrease in the malondialdehyde levels compared to the EtOH group. On the other hand, the glutathione contents in the treatment groups were significantly higher than ethanol group. Fluctuations in antioxidant defense enzyme activities were determined. PO leaf might have a protective role against EtOH-induced testicular damage in rats by inducing apoptosis via the upregulation of eNOS expression and preventing lipid peroxidation.
Histopathological and Biochemical Alterations in the Adrenal Gland of Male Rats Exposed To Bisphenol F
(Pleiades Publishing inc, 2024) Kaptaner, B.; Dogan, A.; Yilmaz, C.; Aykut, H.; Dogan, E.; Babat, C. Fidan; Donmez, F.
Bisphenol F (BPF) is a bisphenol A derivative that is widely used in the manufacturing of industrial and consumer products. The presented study was conducted to explore the adverse effects of BPF on the adrenal glands of rats. Toward this aim, twenty-eight male albino Wistar rats were randomly divided into four groups, each containing seven individuals. The animals were administered a vehicle (control) or BPF at 3 different doses comprising 20, 100, and 500 mg/kg of body weight (bw) via oral gavage for 28 days. Next, the effects of BPF on the gland were evaluated in terms of histopathological alterations, serum adrenocorticotropic hormone (ACTH) and cortisol levels, and the contents of reduced glutathione (GSH) and malondialdehyde (MDA). The zona fasciculata of the adrenal cortex showed prominent histopathological lesions such as vacuolar degeneration and necrotic degeneration in the experimental groups. BPF caused noticeable elevations in the serum ACTH and cortisol levels. Moreover, decreases in levels of GSH and increments in the contents of MDA were determined, indicating oxidative stress. In conclusion, BPF showed toxic effects on the adrenal gland that may result in disruptions in its vital functions in the body. The results herein suggest that attention should be paid to the utilization of BPF in manufacturing processes due to its harmful effects and risks to the health of organisms.
Investigation of the Effects of Three Different Generations of Fluoroquinolone Derivatives on Antioxidant and Immunotoxic Enzyme Levels in Different Rat Tissues
(Taylor and Francis Ltd., 2022) Donmez, F.; Dogan, A.
Fluoroquinolones (FQs) are synthetic and broad-spectrum antimicrobial drugs derived from nalidixic acid. FQs are used against SARS-CoV-2 in our country, and for the treatment of some urinary tract diseases, gastrointestinal diseases, respiratory tract diseases, sexually transmitted diseases, and dermatological diseases. The present study investigated the effect of 1-,7-,14-day treatments of three different FQ derivatives; ciprofloxacin (CIP) 80 mg/kg/day, levofloxacin (LVX) 40 mg/kg/day, and moxifloxacin (MXF) 40 mg/kg/day, on biochemical parameters, lipid peroxidation, antioxidant enzymes, and immunotoxicity. 72 Wistar albino male rats were distributed to four groups including 18 rats in each group and were sacrificed on three different time points. The 14-day treatment of MXF significantly reduced the levels of aspartate aminotransferase (AST), glucose, reduced glutathione (GSH), malondialdehyde (MDA), catalase (CAT), myeloperoxidase (MPO), adenosine deaminase (ADA), and glutathione peroxidase (GPx). Furthermore, 14-day treatment of LVX increased liver [GSH, MPO, ADA, superoxide dismutase (SOD)], and GSH (erythrocyte) levels; whereas it significantly reduced the levels of AST, TG (triglycerides) and associated parameters levels in all the tissues (MDA), erythrocytes, and liver (MPO, CAT, SOD, GPx). After 14-day treatment of CIP; the erythrocyte levels of GSH, MPO, GPx, and CAT significantly decreased; whereas the levels of glucose, creatinine, MPO (liver), and GST (kidney and erythrocyte) significantly increased. It has been concluded that FQ derivatives used in this experiment did not display any correlation in terms of the efficacies in the different time points and tissues. Thus, it is recommended to use such FQ derivatives considering the duration of use and target tissue. © 2021 Informa UK Limited, trading as Taylor & Francis Group.