Browsing by Author "Dulger, Haluk"

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Activity of Mannitol and Hypertonic Saline Therapy on the Oxidant and Antioxidant System During the Acute Term After Traumatic Brain Injury in the Rats
(Elsevier Science Bv, 2007) Yilmaz, Nebi; Dulger, Haluk; Kiymaz, Nejmi; Yilmaz, Cahide; Gudu, Burhan O.; Demir, Ismail
In this study, our objective is to investigate the effects of mannitol and 7.5% hypertonic saline (HS) therapy on the levels of malondialdehyde (MDA), catalase and glutathione peroxidase (GSH-Px) in the early stages of experimental head traumas in rats. Rats included in the study were divided into four groups: Group I Control, Group II Trauma, Group III Mannitol, and Group IV 7.5% Hypertonic Saline. Rats in Group 11 were subject to head trauma only. Mannitol was injected intraperitoneally to rats in Group III after head trauma and 7.5% HS was injected intraperitoneally to rats in Group IV after head trauma. Rats were sacrificed 4 h after administration of mannitol. or 7.5% HS, and the levels of MDA catalase and GSH-Px in brain tissues extracted from rats were determined. MDA levels in the trauma group were significantly increased compared with the control group (p<0.01), whereas there was a reduction in catalase and GSH-Px levels, although these differences were not significant. By contrast, in the mannitol group, MDA, catalase and GSH-Px levels were lower than the levels in the trauma group, and these reductions were statistically significant (p<0.05). The MDA, catalase and GSH-Px levels of the 7.5% HS group were lower than those of the trauma group; however, this reduction was not statistically significant. It was concluded that mannitol and 7.5% HS therapies that are used to reduce intracranial pressure and to increase the use of catalase, an antioxidant enzyme, and GSH-Px, are likely to reduce cellular damage by reducing the formation of MDA, the levels of which are known to be indicative of cellular level oxidant damage. (c) 2007 Elsevier B.V. All rights reserved.
Do Cardiac Neuropeptides Play a Role in the Occurrence of Atrial Fibrillation After Coronary Bypass Surgery
(Elsevier Science inc, 2007) Guler, Niyazi; Ozkara, Cenap; Dulger, Haluk; Kutay, Veysel; Sahin, Musa; Erbilen, Enver; Gumrukcuoglu, Hasan Ali
Background. One of the potential mechanisms to explain the occurrence of postoperative atrial fibrillation (AF) is imbalance of autonomic nervous system tone. The myocardium is innervated not only by cholinergic and adrenergic nerves but also by peptidergic nerves that synthesize and secrete neuropeptides. To investigate the possible role of cardiac neuropeptides in the development of AF after coronary artery bypass grafting (CABG), we analyzed the plasma levels of substance P (SubP), neuropeptide Y (NPY), and angiotensin II (Ang II) in patients who underwent elective on-pump CABG. Methods. This prospective study group included 83 consecutive patients scheduled for elective, on-pump CABG. Depressed left ventricular (LV)function ( ejection fraction [EF]less than 0.30), concomitant cardiac procedures, history of atrial fibrillation, second or third degree atrioventricular block, implanted pacemaker, postoperative myocardial infarction, use of class I or III antiarrhythmic drug, and hemodynamic deterioration were exclusion criteria. Preoperative and postoperative serum levels of SubP, NPY, and AngII were measured by radioimmunoassay technique. Results. Postoperative AF occurred in 27 patients (32.5%). Using multivariate logistic regression analyses, only a decrease in SubP level ( odds ratio [ OR] = 1.87, 95% confidence interval [CI] = 0.767 to 0.99, p = 0.031) and an increase in AngII level ( OR = 2.61, 95% CI = 1.002 to 1.021, p = 0.023) after CABG were found to be independently associated with AF. Increased age ( p = 0.02), diabetes mellitus ( p = 0.023), preoperative use of beta blocker ( p = 0.024), proximal right coronary artery involvement ( p = 0.024), low preoperative sodium levels ( p = 0.023), low LVEF ( p = 0.013), and increased mitral E wave deceleration time ( p = 0.044) were also associated with AF. Conclusions. These results indicate that the increase in AngII and the decrease in SubP after CABG may play a role in the occurrence of postoperative AF. Further studies are needed to define the physiologic and pathologic relevance of these substances at the occurrence of AF in patients who undergo CABG. (c) 2007 by The Society of Thoracic Surgeons.
Effect of Depot Oral Cholecalciferol Treatment on Secondary Hyperparathyroidism in Stage 3 and Stage 4 Chronic Kidney Diseases Patients
(Taylor & Francis Ltd, 2008) Dogan, Ekrem; Erkoc, Reha; Sayarlioglu, Hayriye; Soyoral, Yasemin; Dulger, Haluk
By the time patients require dialysis replacement therapy, nearly all chronic kidney diseases (CKD) patients are affected with uremic bone diseases. High-turnover osteodystrophy can be prevented; patients with CKD should be monitored for imbalances in calcidiol (25 OH vitamin D), calcium, and phosphate homeostasis. We aimed to determine the effect of a monthly oral 300,000 IU vitamin D-3 (cholecalciferol) supplementation on the uremic bone diseases (UBD) markers such as iPTH and alkaline phosphatase in CKD patients. Among a total of 70 patients under treatment in the nephrology unit, 40 predialysis CKD patients (mean age of 49 14, male/female 20/20) were included the study. The patients were randomly divided into two groups. Treatment group included 20 patients (mean age of 51 +/- 14, male/female 9/11), and the control group comprised 20 patients (mean age of 47 +/- 14, male/female 9/11). Treatment group patients were given a single dose of Devit3 ampoule (300,000 U cholecalciferol) per month orally way. Patients in the control group did not take any vitamin D for a month. The level of calcidiol was lower than normal range in two groups. After a month, treatment group patient's calcidiol increased statistically significant (6.8 +/- 3.5 to 17.8 +/- 21.4 ng/mL, p < 0.001). After a month, iPTH level decreased in the treatment group statistically significantly (368 +/- 274 to 279 +/- 179 pg/ml, p < 0.001). At the 30(th) day of the treatment, in 9/20 of the treatment group patients (45%), the iPTH value decreased at least 30% (p < 0.001). We suggest that oral depot cholecalciferol treatment causes a statistically significant decrease of serum iPTH level but does not cause a statistically significant change in Ca, P, ratio of CaxP, or urinary calcium creatinine rate in UBD predialysis CKD. This treatment can be used safely for the predialysis CKD patients, along with the cautious control of serum calcium and phosphor.
The Effects of High-Rate Frequency Modulation Treatment on Malondialdehyde in Diabetic Polineuropaty
(Blackwell Publishing, 2006) Kavak, Tuyana; Kavak, Servet; Emre, Mustafa; Tulgar, Metin; Dulger, Haluk; Cankaya, Soner
Homocysteine Levels and Lipid Profile in Hemodialysis Patients
(Ortadogu Ad Pres & Publ Co, 2007) Dulger, Haluk; Gur, Tugba; Sayarlioglu, Hayriye; Sekeroglu, Ramazan; Erkoc, Reha; Begenik, Hueseyin
Objective: The aim of this study was to investigate the levels of homocysteine and lipid profiles in patients included in hemodialysis programs for chronic renal failure (CRF). Material and Methods: The patients were divided into 2 groups and each group consisted of 30 patients, leading to a total of 60 patients. On the other hand, 20 healthy people comprised the control group. The first group (nontreated CRF) included new patients without a previous history of dialysis or any medical treatment; the second group (treated CRF) included patients who have received medical treatment [vitamin 13,2 (1 mg/month) and folic acid (15 mg/week)] and dialysis for at least the last 5 years. Serum total homocysteine, vitamin B-12, folic acid, creatinine, triglyceride, total cholesterol, HDL, LDL, VLDL, cholesterol levels were measured in all patients and the control group. Results: Levels of homocysteine were high in group I and group 2 patients (respectively p < 0.01, p < 0.05), whereas serum HDL cholesterol levels in group 2 were low compared to those in the control group (p < 0.01). In addition, a negative correlation was observed between homocysteine and folic acid levels in group 2 patients (r= -0.48, p < 0.01). Conclusion: The results of this study showed that homocysteine levels in CRF increased and this increase was lower in group 2 patients. Administration of folic acid reduced the levels of homocycstein. Thus, we Suggest that folic acid may be a significant factor to prevent the progression of chronic renal failure.
The Importance of Oxidative Stress in Patients With Chronic Renal Failure Whose Hypertension Is Treated With Peritoneal Dialysis
(Wiley, 2011) Demirci, Serafettin; Sekeroglu, Mehmet Ramazan; Noyan, Tevfik; Koceroglu, Rusen; Soyoral, Yasemin Usul; Dulger, Haluk; Erkoc, Reha
Increased oxidative stress is a well-known phenomenon in dialysis patients. However, the contribution of hypertension to the oxidative stress in peritoneal dialysis patients has not yet been assessed. The present study aimed to investigate if hypertension had an additional effect on oxidative stress in peritoneal dialysis patients. A total of 50 patients treated with peritoneal dialysis were divided into two groups: The patients with mean of last three blood pressure results as 135/90 mmHg and above were considered hypertensive, the patients with lower blood pressure were considered normotensive. The control group included 25 healthy individuals. Serum malondialdehyde (MDA), advanced oxidation protein product (AOPP), myeloperoxidase (MPO), catalase (CAT) and glutathione peroxidase (GSH-Px) levels were measured in all groups. MDA level, an indicator of lipid peroxidation, was significantly higher in the hypertensive group compared to the control group, while the increase in the normotensive group was not significant. However, the difference between the hypertensive and normotensive groups was significant. The levels of AOPP, an indicator of protein oxidation level, and MPO, an indicator of neutrophil activation, were not different between the groups, while the activities of antioxidant CAT and GSH-Px decreased in both normotensive and hypertensive groups compared to the control group, and there was no significant difference between the patient groups. This study shows that both normotensive and hypertensive peritoneal dialysis patients have increased-oxidative stress and decreased antioxidant levels and hypertension might have an additional effect on oxidative stress by increasing MDA level in peritoneal dialysis patients. Copyright (C) 2011 John Wiley & Sons, Ltd.
Investigation of the Relationship Between Serum Levels of Cotinine and the Renal Function in Active and Passive Smokers
(informa Healthcare, 2011) Dulger, Haluk; Donder, Ahmet; Sekeroglu, Mehmet Ramazan; Erkoc, Reha; Ozbay, Bulent
Objective: We have investigated the effects of active and passive smoking on renal functions in terms of glomerular filtration rate, microalbuminuria, and beta-2 microglobulin excretion. Design and method: The volunteers included in this study were classified into three groups as active smokers (n = 24), passive smokers (n = 20), and controls (n = 20). Blood and urine samples were collected from all groups. Serum glucose, urea, creatinine, and cotinine levels in the collected blood samples were measured. Also, microalbumin, beta-2 microglobulin, and creatinine levels were measured in the collected urine samples. Results: Serum cotinine levels were found to be higher in both passive and active smokers when compared with controls (p < 0.01), whereas urinary microalbumin and creatinine levels were significantly higher in active smokers (p < 0.01). The urinary microalbumin/creatinine ratio was significantly increased in both active and passive smokers compared with controls. Conclusion: The kidney and the glomerular functions may be affected even by passive smoking. In addition, increased microalbumin/creatinine ratio may be a sign of increased atherosclerosis risk in these persons.
Lipid Peroxidation in Patients With Brain Tumor
(Taylor & Francis Ltd, 2006) Yilmaz, Nebi; Dulger, Haluk; Kiymaz, Nejmi; Yilmaz, Cahide; Bayram, Irfan; Ragip, Balahoroglu; Oger, Muzaffer
Molecular and genetic signatures may predict brain tumor behavior and may soon guide tumor classification, diagnosis, and tumor-specific treatment strategies. Free oxygen radicals ( FOR) are thought to take part in oncogenesis and cellular differentiation. This article explored the state of FORs and antioxidant system in patients with cerebral tumor. The serum concentrations of malondialdehyde (MDA), catalase, and glutathione peroxidase (GSH-Px) enzyme activities were measured in the serum of 35 patients with cerebral tumors ( 21 glioma, 14 meningioma) and 11 controls. MDA measurement was done with fluorometric method and catalase and GSH-Px enzyme activities were done with photometric method. Mean serum MDA levels, catalase, and GSH-Px enzyme activities were significantly higher for both glial and meningiomal tumor cases when compared to controls (p < .05). There is no significant difference between glioma and meningioma groups in terms of the aforementioned parameters ( p > .05). In conclusion, lipid peroxidation and antioxidant enzymes as assessed by MDA, catalase, and GSH-Px were increased in patients with brain tumors, for this respect there is no difference between gliomas and meningiomas.
Serum Cytokines and Bone Metabolism in Patients With Thyroid Dysfunction
(Health Communications inc, 2006) Sekeroglu, M. Ramazan; Altun, Z. Busra; Algun, Ekrem; Dulger, Haluk; Noyan, Tevfik; Balaharoglu, Ragip; Ozturk, Mustafa
Hyperthyroidism is associated with increased bone turnover. Besides the hormones of calcium metabolism , locally produced factors are important in maintaining normal bone metabolism. Interleukin-6 (IL-6), in particular, has a major influence on bone turnover. In this study, serum IL-6 and tumor necrosis factor-alpha (TNF-alpha) levels, as well as bone turnover markers and relationships between them, were investigated in hyperthyroidism and hypothyroidism. A total of 20 female patients with hyperthyroidism, 15 with subclinical hyperthyroidism, 16 with hypothyroidism, and 15 with subclinical hypothyroidism constituted the patient groups. In all, 15 age-matched healthy female volunteers were recruited as controls. When compared with controls, serum TNF-a levels showed no significant difference in any of the patient groups (P >.05). In the groups with hyperthyroidism and subclinical hyperthyroidism, IL-6 levels were significantly higher compared with control group values (P <.05). Hyperthyroid patients showed higher levels of alkaline phosphatase (ALP) and osteocalcin, and a higher urinary deoxypyridinoline/creatinine ratio, compared with controls (P <.05). In subclinical hyperthyroidism, only ALP was found to be higher compared with control values. No significant correlations were made in any group between serum IL-6 or TNF-a level and bone turnover markers. Results suggest that serum IL-6 level and markers of bone turnover rate seem to be increased in hyperthyroidism. This finding may support the role of IL-6 in induction of bone turnover in hyperthyroid states.
The Serum Interleukin-6 and Tumor Necrosis Factor-Α Levels and Their Relationship With Antithrombin-Iii and Von Willebrand Factor in Preeclampsia
(Galenos Yayincilik, 2006) Noyan, Tevfik; Bursal, Ercan; Sekeroglu, Mehmet Ramazan; Dulger, Haluk; Kamaci, Mansur
Objective: Preeclampsia, characterized by changes in the placenta and uteroplacental vasculature, is the most frequent complication of pregnancy. It is argued that an overproduction of placental cytokines may be associated with the pathophysiological changes found in preeclampsia. In this study, it is aimed to determine the levels of both pro-inflammatory (tumor necrosis factor-alpha) and immunoregulatory (interleukin-6) cytokines and their relationships with von Willebrand factor and anti-thrombin III from both preeclamptic and healthy pregnant women. Material and Methods: Twenty-five pregnant women with preeclampsia and 25 healthy pregnant women were included in the study. The fasting blood samples were obtained at eight o'clock in the morning from the both groups and interleukin-6, tumor necrosis factor-alpha, von Willebrand factor and anti-thrombin III levels were measured. From the preeclamptic patients blood samples were obtained at twelve o'clock midnight to determine the diurnal variation in these two cytokine levels. Results: As compared to healthy pregnant group, levels of interleukin-6 and anti-thrombin III were significantly lower and von Willebrand factor level was significantly higher in the preeclamptic group (p< 0.05). Tumor necrosis factor-alpha level did not differ significantly between the two groups. Interleukin-6 and tumor necrosis factor-alpha levels did not show significant diurnal variation in preeclamptic group. A significant positive correlation was found between the tumor necrosis factor-alpha levels measured in the morning and night samples in the preeclamptic group (p< 0.01, r=0.701). Also, a significant positive correlation was found between the tumor necrosis factor-alpha and von Willebrand factor levels in healthy pregnant group (p< 0.05, r=0.648). Conclusions: These findings suggest that preeclampsia is associated with decreased interleukin-6, and anti-thrombin III, and with increased von Willebrand factor levels.
Serum Lipid Concentrations in Obsessive-Compulsive Disorder Patients With and Without Panic Attacks
(Canadian Psychiatric Assoc, 2004) Agargun, Mehmet Yucel; Dulger, Haluk; Inci, Rifat; Kara, Hayrettin; Ozer, Omer Akil; Sekeroglu, Mehmet Ramazan; Besiroglu, Lutfullah
Objective: To examine serum lipid levels in patients with obsessive-compulsive disorder (OCD) and to test whether panic symptoms affect lipid concentrations in OCD patients. Methods: We assessed 33 OCD patients and 33 healthy control subjects matched for sex and age. Results: OCD patients had higher low-density lipoprotein, very-low-density lipoprotein, and tryglyceride levels, but lower high-density lipoprotein levels, than normal control subjects. We also found that only OCD patients with panic attacks had higher serum lipid concentrations, compared with normal control subjects. Serum lipid levels of pure OCD patients did not differ from control values. Conclusion: These findings suggest that high serum lipid concentrations are related to panic anxiety rather than other symptoms of the illness. (Can J Psychiatry 2004;49:776-778)
Vitamin D-Dependent Rickets: Eight Cases
(Modestum Ltd, 2016) Cesur, Yasar; Yuca, Sevil Ari; Bektas, Selcuk; Yilmaz, Cahide; Dulger, Haluk; Temel, Hayrettin
Objective: Vitamin D is essential for bone development and health, and deficiency resulting in rickets and skeletal deformities is seen mainly during rapid growth. Hereditary vitamin D dependent rickets type I and type II rickets is a very rare form of rickets, characterized by 1-alpha-hydroxylase deficiency or end-organ resistance to vitamin D. We aimed to investigate, clinical and laboratory characteristics of eight cases with Vitamin D-dependent rickets (VDRR). Method: The mean age of patients during diagnosis was 2.6 years. Excluding one patient, others were males (87.5%). Results: Mean laboratory values during referral was calcium 7.5 +/- 1,5 mg/dl, phosphorus 4 +/- 1.2 mg/dl, alkaline phosphatase (ALP) 1679 +/- 641 U/L and parathyroid hormone (PTH) 524 +/- 498 pg/ml. Patients received 1.2 mu g/kg/day calcitriol. During follow-ups serum ALP and PTH values of patients turned to normal levels. Conclusion: In rickets, cases with persistent increased serum ALP and PTH levels it will be appropriate to investigate serum 25 (OH) D levels in cases diagnosed with vitamin D-dependent rickets even though hypocalcaemia is absent. Administration of adequate doses of calcitriol in some cases is able to clinical and laboratory values return to normal.