Browsing by Author "Duman, Kendal Erdem"

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Ameliorative Role of Cyanus Depressus (M.bieb.) Sojak Plant Extract Against Diabetes-Associated Oxidative-Stress Liver, Kidney, and Pancreas Damage in Rats
(Wiley, 2022) Duman, Kendal Erdem; Dogan, Abdulahad; Kaptaner, Burak
In this original article, we aimed to assess the ameliorative role of Cyanus depressus (CD) plant ethanolic extract treatment of streptozotocin (STZ)-induced liver, kidney, and pancreas damage in rats. The rats were divided into five groups (n = 7): control, CD, Diabetes mellitus (DM), DM + CD, and DM + glibenclamide (Gly). The DM groups were injected with a single dose of 50 mg/kg STZ intraperitoneally (i.p.). While the CD and DM + CD groups received 400 mg/kg/day intragastrically for 21 days, the DM + Gly group received 3 mg/kg/day of Gly intragastrically throughout the experiment. Statistically significance was accepted as p < .05. According to our liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) data, quinic acid, cosmosiin, nicotiflorin, apigenin, and protocatechuic acid were the major compounds, in descending order. Weekly blood glucose, serum glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH) and urea, malondialdehyde (MDA) (liver and pancreas), and blood glycosylated hemoglobin % (HbA1c %) were significantly decreased, whereas finally live body weights (LBWs), reduced glutathione (GSH), glutathione S-transferase (GST) and catalase (CAT) (pancreas), and pancreatic islet diameter and area were increased significantly in the CD-treated diabetic group. Moreover, CD administration was found to be effective in the protection of the histology of the liver, kidneys, and pancreatic islets in the STZ-induced rats. Consequently, we concluded that CD administration reduces hyperglycemia, oxidative stress, and histopathology in STZ-induced experimental rats by improving antioxidant defenses. Practical applications Today, the prevalence of diabetes is increasing rapidly throughout the world and it causes complications such as kidney damage, blindness, amputations, and cardiovascular diseases. Despite medical technological advances, people's interest in medicinal herbal products is gradually increasing. Biochemical and histopathological findings showed that the use of the plant CD at the determined dose (400 mg/kg/day) in rats with DM by STZ had strong antioxidant and antidiabetic effects. CD may have a drug potential in preventing DM and its complications because of its phytochemical content including some phenolic acids such as quinic acid, cosmosiin, nicotiflorin, apigenin, and protocatechuic acid. Isolation of bioactive compounds from CD and investigation of their therapeutic effects could be planned as further studies.
Investigation of the Antidiabetic and Antioxidant Effects of Cornflower (cyanus Depressus) Plant Extract Against Streptozotocin-Induced in Diabetic
(2021) Duman, Kendal Erdem; Doğan, Abdulahad; Kaptaner, Burak
Bu çalışmada, streptozotosin (STZ) ile diyabet oluşturulan ratlarda Cyanus depressus (CD) çiçeği liyofilize etanolik ekstresinin antioksidan ve antidiyabetik etkileri araştırıldı. Çalışmada otuz beş rat 7 gruba ayrıldı; Grup I: Kontrol grubu, Grup II: CD-400 mg/kg grubu (C. depressus 400 mg/kg çiçek ekstresi uygulanan ratlar); Grup III: DM grubu (50 mg/kg tek doz STZ intraperitonal yolla uygulanan ratlar); Grup IV: DM + CD-400 mg/kg grubu (C. depressus çiçek ekstresi 400 mg/kg uygulanan diyabetik ratlar); Grup V: DM + Gly-3 mg/kg grubu (Antidiyabetik ilaç glibenklamid 3 mg/kg uygulanan diyabetik ratlar). Yirmi bir günlük muameleler sonrasında; CD çiçek ekstresinin antidiyabetik rolünun belirlenebilmesi için; serum biyokimyasal parametrelerden glukoz, glikolize hemoglobin (HbA1c), aspartat amino transferaz (AST), alanin amino transferaz (ALT), laktat dehidrogenaz (LDH), kreatinin, üre, trigliserit (TG), kolestreol (TC) ve yüksek yoğunluklu lipoprotein (HDL) ölçüldü. Ayrıca karaciğer, böbrek ve pankreas dokularında antioksidan savunma unsurlarından glutatyon-S-transferaz (GST), glutatyon reduktaz (GR) ve katalaz (CAT) aktiviteleri ile redükte glutatyon seviyeleri ve malondialdehit (MDA) içerikleri değerlendirildi. Daha sonra serum insulin ve C-peptid hormon düzeyleri ölçüldü. Karaciğer, böbrek ve pankreas dokularında histolojik incelemeler yapıldı ve pankraesta adacık çapı ve adacık alanı hesaplandı. Elde edilen bulgulara göre CD ekstresi ile tedavi edilen rat grupların, diyabetik gruba kıyasla, biyokimyasal ve MDA parametrelerinde düşüş, antioksidan enzim aktivitesinde ve GSH (karaciğer hariç) düzeyinde artış istatistiksel olarak anlamlı bulundu. Yapılan histolojik incelemelerde, DM grubunda gözlenen lezyonların CD çiçek ekstresi uygulanan gruba göre hafifletildiği gözlenmiştir. Ayrıca, pankreas adacık çapı ve alanı DM grubuna kıyasla diğer tedavi gruplarında anlamlı artışlar gösterdiği belirlendi. Sonuç olarak, CD çiçek esktresi diyabet ve diyabetle ilişkili komplikasyonların tedavisinde umut verici bir terapötik ajanı temsil edebilir