Browsing by Author "Duz, E."

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The Effect of Platelet Rich Plasma on Wound Healing in the Repair of Experimental Penile Fractures
(Agricultural Research Communication Centre, 2022) Duz, E.; Beger, B.; Saglam, K.; Yildirim, S.
Background: Penile fractures are caused by the rupture of the tunica albuginea and the underlying layers of corpus cavernosum as a result of the exposure of the erect penis to blunt trauma. Although conservative treatment methods have been recommended in former studies, recent studies favor early surgical options. Platelet-rich plasma (PRP) is a blood product rich in cytokines and several growth factors, obtained from the patient's own blood commonly used to stimulate healing in acute and chronic wound treatments. The efficiency of homologous PRP treatment in rats with penile fractures have been assessed in terms of clinical manifestation and histopathological findings in the present study. Methods: Thirty-five rats were randomly divided into five groups, each containing seven rats as follows: 1. Conservative group: Following the formation of penile fracture, medical interventions were not initiated on fracture area and the wound was left for secondary healing. 2. Bandage group: Following the formation of penile fracture, self-adherent elastic bandage (3 M Coban) was applied to the fracture area for three days. 3. Primary repair group: Following the formation of a penile fracture, the lacerated area was repaired with 7/0 vicryl primary sutures. 4. Primary repair + Homologous Platelet Rich Plasma group: Before the operation, 10 cc of blood was taken from one rat under general anesthesia by intracardiac puncture and PRP material was prepared from this blood. Homologous PRP was topically administered on the wound in the fracture area and then the penile fracture was repaired with 7/0 vicryl as simple separate stitches. 5. Non-operative control group: Surgical procedures were not applied to this group; it was used as the control group. Result: The effect of the PRP treatments on the surgical repair options in the rats with penile fractures was investigated. The present findings revealed that following the repair with conservative treatments, the fracture format persisted and various complications like infection, oedema, excessive fibrosis and hyperemia were encountered. On the other hand, the best healing was achieved in primary repair + PRP group in terms of clinical manifestation and histopathological findings.
Effects of Sildenafil Citrate on the Hematological Parameters in the Early Phase of Wound Healing in Diabetic Rats
(Academic Journals inc, 2011) Tas, A.; Karasu, A.; Comba, B.; Aksu, D. S.; Duz, E.; Tanritanir, P.
In this study Investigation of effects of sildenafil citrate on some of the hematological parameters in the early phase of wound healing in diabetic rats was aimed in this study. Total 30 rats were used in the study. Rats were divided into three groups equal in number (control-non diabetic, diabetic and diabetic+Sc). While no treatment was given to the control-non diabetic and diabetic groups, sildenafil citrate was given to the diabetic+Sc group intraperitoneally with a dosage of 0.7 mg/kg/day dosage for 3 days after creating wounds: under ketamine anesthesia, a circular, full thickness, standard wound was created on the back of each rat by using a 0.8 cm sterile punch. Cardiac blood was drawn in tubes containing EDTA from each group before the creation of wounds and on days 1, 2 and 3 following the creation of wounds. Differential leukocyte counts were determined in blood samples containing anticoagulant. For this purpose, blood smears were prepared from blood samples and stained with Giemsa method. It was found that, while neutrophil counts increased in all the groups during the wound-healing periods with the increasing number of days, lymphocyte counts reduced and in control and Sc+diabetic groups, monocyte counts increased. As a result, it was concluded that Sc plays an important role in eliminating the pressure on the cellular activities in the early period of wound healing and continuance of the inflammatory process and wound healing within normal limits and also that this could be demonstrated with hematological analyses.
Extremity Problems in Ostrich Chicks and Their Treatment
(2009) Asian, L.; Genccelep, M.; Karasu, A.; Duz, E.; Alkan, I.; Bakir, B.
Extremity problems in 120 ostrich chicks were investigated in this study. Fledglings were followed for 1 year and extremity problems were evaluated as congenital or acquired. In the study, congenital lower extremity problems were found in 17.5% of the cases (both extremities extended sideways m 10 cases, 1 extremity extended sideways in 3 cases, one leg extended forward and the other back in 4 cases, big toe retrovert in 2 cases, 5th toe deviated towards underneath of the big toe, totaling to 21 cases) and acquired lower extremity problems in 19.64% of the cases (tibiotarsal luxation in 6 cases, tarsal bone fracture in 1 case, fractures in radius-ulna in 1 case, injuries of the extremities in 9 cases and arthritis in the tarsal joint in 2 cases and in proximal mterphalangeal joint in 3 cases, totaling to 22 cases). Extremity problems were found in 43 (35.83%) ostrich chicks out of 120 (congenital in 21 cases and acquired in 22). Of these 43 cases, it was observed that 29 (67.44%) were healed with the treatment applied, whereas, 14 (32.56%) did not heal. It was concluded in the study that the extremity problems causing great economic losses in ostrich husbandry can be minimized with prevention, early diagnosis and proper treatment. © Medwell Journals, 2009.
Histopathological and Immunohistochemical Study of the Effect of Sildenafil Citrate, Vitamin A, Vitamin C and Vitamin E on Wound Healing in Alloxan-Induced Diabetic Rats
(Univ West indies Faculty Medical Sciences, 2021) Tas, A.; Karasu, A.; Yener, Z.; Yildirim, S.; Atasoy, N.; Duz, E.; Yavuz, E.
Objective: To examine the impact of sildenafil citrate, vitamin A (Vit A), vitamin C (Vt C) and vitamin E (Vit E) on wound healing in a rat model of diabetes (n = 100). Methods: Diabetes was induced in the rats by intraperitoneal (i.p.) administration of alloxan. After anaesthesia, a standard wound was created on the back of each rat using a 10-mm sterile punch. The rats were randomly divided into 10 groups (n = 10 in each), as follows: normal saline, glibenclamide, insulin, sildenafil, Vit A, Vit C, Vit E, Vit A + sildenafil, Vit C + sildenafil, Vit E + sildenafil daily for 15 days. The rats were sacrificed after being anaesthetized 3, 7 and 15 days later Wounded skin tissue samples were collected for histopathological and immunohistochemical analyses. Results: On the 7th day, epithelial regeneration was completed in groups 8 and 9. Angiogenesis was insufficient in group 2. In terms of connective tissue proliferation, partially matured connective tissue was observed in group 4. On the 15th day of the study, groups 8, 9 and 10 had mature connective tissue. However; group 1 still had exudate-containing neutrophils. Immunohistochemically, on the 3rd day, the level of inducible nitric oxide synthase (iNOS) reactivity was intense in macrophages and neutrophils surrounding the wound edges in groups 4, 8, 9 and 10. The level of iNOS reactivity was moderate in group 6 and less distinct in groups 1, 2, 3, 5 and 7. Conclusion: Sildenafil citrate, together with Vit A and Vit C, is beneficial in wound healing of diabetic rats.
The Investigation on the Effect of the Vegetal Origin Ankaferd Blood Stopper in Experimental Intra-Abdominal Surgery Over Rabbits
(2010) Duz, E.; Asian, L.; Alkan, I.; Bayram, I.; Kaya, A.; Ayhan, H.; Özer, E.
With this study, it is aimed to conduct a research on the haemostatic effects of the herbal originated blood stopper product which is titled as Ankaferd Blood Stopper (ABS) in intra-abdominal surgery in rabbits. The materials of the study consist of 20 healthy adult New Zeeland rabbits, 10 male and 10 female ones. The rabbits were divided into two groups as working group and control group. The left abdominal part of rabbits was incised by 5 cm, their skin, cutaneous connective tissue and muscles were incised, respectively. About 1-2 mL gauzed bandage tampons which were saturated with Ankaferd Blood Stopper applied on all of 10 cases. The time passed for the blood to stop was measured by the chronometer. Moreover, bleeding spots with 1 cm length and 5 mm depth were made by bistoury on the liver and spleen. The tampons saturated with ABS were applied on the liver and spleen for 90 sec with medium pressure. The tampons with the dimensions of 4X4 cm were applied on bleeding spots of the control group with medium pressure. The time passed for the blood to stop was recorded. The time passed for blood stopping was compared. With Ankaferd Blood Stopper, the haemostasis was achieved within 9 sec for application in skm, 19 sec in coetaneous connective tissue, 42 sec in muscles, 147 sec in liver, 162 sec in spleen in experimental group while haemostasis was achieved within 220 sec for application in skin, 256 sec in coetaneous connective tissue, 238 sec in muscles, 550 sec in liver and 400 sec in spleen. As a conclusion, when the blood stopper product which is known as Ankaferd Blood Stopper is compared to the control group in external bleeding, it is seen that it achieves an effective haemostasis. © Medwell Journals, 2010.
A New Enema for Treatment of Intussusception With Hydrostatic Reduction: Olive Oil
(Wolters Kluwer Medknow Publications, 2019) Beger, B.; Duz, E.; Kizilyildiz, B.; Akdeniz, H.; Melek, M.; Agengin, K.; Sonmez, B.
Objectives: Intussusception is routinely treated using ultrasound-guided hydrostatic reduction (USGHR) with normal saline in our paediatric surgery department. With this study, olive oil was added to normal saline in ultrasound-guided reduction of intussusception. Materials and Methods: Forty patients who were diagnosed and treated for intussusception in Van Yuzuncu Yil University Faculty of Medicine Pediatric Surgery Department from March 2017 to May 2017 were included in the study. During this retrospective study, randomly chosen 20 patients that treated with USGHR using normal saline were marked as Group 1. Moreover, 20 patients that treated with USGHR using a mixture of olive oil and saline (10% olive oil 90% normal saline) were marked as Group 2. Patients' age, gender, symptoms, treatment techniques, complications and hospitalisation periods were retrospectively noted. Results: Forty patients were included in the study. In Group 1, 14 patients were treated in the first session, 4 patients were treated in the second session and 2 patients required laparotomy. Mean reduction time in this group was 15 min mean fluid volume used in each reduction was 80 ml/kg and mean hospitalisation period was 38 h. In Group 2, 19 patients were treated in the first session, and only 1 patient required a second session. Mean reduction time was 12 min, used fluid volume was 58 ml/kg for each reduction and hospitalisation period was 24 h. Conclusion: The average volume of fluid used for reduction, average reduction time, numbers of recurrent reductions and hospitalisation were less when a mixture of olive oil and normal saline were used in comparison with when normal saline was used alone to reduction the intussusception under ultrasound guidance. Using olive oil mixed with normal saline as a new enema fluid is likely to increase the success rate of ultrasound-guided reduction of intussusception. © 2019 Wolters Kluwer Medknow Publications. All rights reserved.