Browsing by Author "Ekin, S"

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Comparative Study of Total Protein, and Total and Lipid-Associated Serum Sialic Acid Levels in Patients With Type 2 Diabetes Mellitus
(Wiley-liss, 2003) Ekin, S; Meral, I; Gunduz, H; Mert, N
The aim of the present study was to investigate the serum total protein (TP), total sialic acid (TSA), lipid-associated sialic acid (LSA), LSA/TP, and LSA/TP values in type 2 diabetes mellitus (DM) patients. Two study groups (healthy controls and type 2 DM subjects) were examined. For the type 2 DM group, 120 patients (60 females and 60 males) who had been diagnosed and treated for type 2 DM in the Yuzuncu Yil University Hospital, Van, Turkey, were selected consecutively. Forty healthy individuals (20 females and 20 males) were selected from hospital staff and other outpatient clinics to serve as the control group. They were matched for age, sex, body mass index, and smoking status. None of the participants had taken vitamin or mineral supplements for at least 2 weeks before sampling. To determine serum glucose, TP, TSA, and LSA levels, blood samples were drawn after all of the subjects fasted overnight. It was found that diabetics had higher TSA, LSA, TSA/TP, and LSA/TP levels than controls. However, the TP levels were not significantly different between the groups. Our results showed that TSA, LSA, TSA/TP, and LSA/TP have interactive connections with DM. These parameters can be used as a diagnostic index for patients with DM. J. Clin. Lab. Anal. 17:124-126, 2003. (C) 2003 Wiley-Liss, Inc.
Concentration of Zn, Cu, Mn and Mg in Cattle With Natural Psoroptes Bovis Scabiei
(indian veterinary Journal, 2005) Kozat, S; Ekin, S; Yuksek, N; Kaya, A; Agaoglu, Z
Scabies is caused by Psoroptes bovis. The most cited causes of the disease are the over crowded accommodation of the animals in the barns, malnutrition and imbalanced feeding (Sargison et al., 1995; Sekin et al., 1998). Trace elements and minerals are of a prominent value in terms of increasing the resistance against the disease in the biological organisms, causing severe clinical conditions. in case of their shortage or excess (Underwood and Suttle, 2001). The aim of this study is to find out the levels of zinc (Zn), copper (Cu) Manganese (Mn) and Magnesium (Mg) in Psoroptes scabiei in cattle.
Effects of Dexamethasone on Trace Elements and Serum Protein Patterns Following Brain Trauma in Rats
(Humana Press inc, 2005) Ekin, S; Berber, I; Kiymaz, N
The effect of dexamethasone (Dxm) on trace elements and serum protein patterns was investigated in male Sprague-Dawley rats subjected to brain trauma. After 6- or 24 h of the traumatic incident, the level of serum copper was significantly higher in the Dxm-treated rats, compared to controls (p < 0.05). The corresponding levels of zinc and iron did not show significant differences. The zinc level returned to normal 24 h after trauma. After 6 and 24 h of trauma, the sodium dodecyl sulfate (SDS)-polyacrylamide gel patterns of serum proteins showed that a 41.6-kDa protein was significantly increased in the Dxm-treated animals. Two proteins weighing 26.6 kDa and 55.1 kDa did not show Dxm-induced changes. These results suggest that increases in the copper-zinc ratio and the changes of the 26.6-kDa, 41.6-kDa, and 55.1-kDa proteins might be a useful prognostic indicator for severe traumatic brain injury.
Levels of Some Trace Elements and Rheumatoid Factor in Sheep With Brucellosis
(Humana Press inc, 2004) Ekin, S; Kozat, S; Gunduz, H; Mert, N; Karakaya, C
Brucellosis is a zoonotic disease that is encountered in sheep rather frequently. In this study, 100 sheep diagnosed with brucellosis that had aborts and 40 healthy sheep were used as materials. Analyses for Cu, Zn, Fe, Cr, Ca, Mg, and K were performed with the atomic absorption spectrophotometric method on blood collected from vena jugularis of all the sheep and rheumatoid factor levels were determined by the nephelometry method. Although it was found that Cu, Ca, and rheumatoid factor values in the sera of the sheep with brucellosis were high when compared to the control group (p<0.001, p<0.05, p<0.001, respectively), their serum Zn values were low (p<0.05). No significant changes in serum Cr, Fe, K, and Mg levels were found.
Serum Adenosine Deaminase Activity and Biochemical Parameters in Chronic Fascioliasis in Sheep
(indian veterinary Journal, 2006) Kozat, S; Ekin, S; Kaya, A; Denizhan, V
Serum Sialic Acid Levels and Selected Mineral Status in Patients With Type 2 Diabetes Mellitus
(Humana Press inc, 2003) Ekin, S; Mert, N; Gunduz, H; Meral, I
The aim of the present study was to investigate whether altered serum total sialic acid (TSA), lipid-associated sialic acid (LSA), copper (Cu), manganese (Mn), zinc (Zn), chromium (Cr), iron (Fe), and magnesium (Mg) levels had an interactive connection with diabetes and also whether they were correlated with each other in diabetic patients. Two study groups (control and type 2 diabetic subjects) were included. Two hundred patients (108 female and 92 male), diagnosed and treated for type 2 diabetes in the Yuzuncu Yil University Hospital (Van, Turkey), were selected consecutively to represent type 2 diabetic patients. Fifty healthy individuals (29 female and 21 male) served as the control group matched for age, sex, body mass index, and smoking status were selected from hospital staff and other outpatient clinics. All participants had not taken vitamin or mineral supplements for at least 2 wk before sampling. Blood samples were drawn after an overnight fasting in both groups for the determination of serum glucose, TSA, LSA, Cu, Zn, Mn, Cr, Fe, and Mg. It was found that diabetics had higher TSA, LSA, Fe, Mn, Fe/Zn, and Cu/Zn levels, and lower Zn and Mg levels than those of controls. Although, Cu levels were higher, and Cr levels were lower in total and male diabetic patients, they were not different in female diabetic patients than in controls. The Cu/Fe ratio was lower in total and female diabetic patients, but not different in male diabetic patients than controls. The Zn/Cr ratio, on the other hand, was not different in diabetics than in controls. There was only a positive correlation between Fe-Mn levels in male diabetic patients. There was a negative correlation in LSA-Mn, Fe-Cu, Cu-Fe/Zn, and Mn-Cu/Zn levels in total diabetic patients. There was a positive correlation in TSA-Cr, TSA-Mg, LSA-Cu/Fe, LSA-Zn/Cr levels, and a negative correlation in TSA-Cu/Zn, LSA-Mn, Fe-Cu, Mn-Cu, Cu-Fe/Zn, Fe-cholesterol, and Cr-cholesterol in female diabetic patients. Our results showed that TSA, LSA, and selected minerals have interactive connections with diabetes mellitus (DM). There are also many sex-related positive or negative correlations between the altered parameters in diabetic patients. These parameters might be used as diagnostic index in patients with DM.
A Study on Esterase Activity of Carbonic Anhydrase From Van Lake Fish (Calcalburnus Tarichi)
(Tubitak Scientific & Technological Research Council Turkey, 1999) Testereci, H; Sekin, S; Ekin, S
No study has been reported on carbonic anhydrase enzyme of the fish (Calcalburnus tarichi) living in the carbonated Van Lake. Having partial purification of Carbonic anhydrase from gill and liver of Van Lake fish by ethanol-chloroform and acetone-chloroform, the esterase activity was performed by using p-nitrophenyl acetate as a substrate on spectrophotometer. The effects of the some kinetic parameters (km, Vo, Vmax, Na2CO3 activation, optimum pH etc.) have been examined When Lineweaver-Burk diagram of liver extracts (at 25 degrees C) is examined, ethanol has an apparent competitive inhibition compare to acetone extracts. So Km of the substrate (p-nitrophenyl acetate) is 0.95 mM is for ethanol extract and 0.54 mM of that is found for acetone extracts. In two extracts from gill (at 25 degrees C), ethanol seems to have a non-competitive inhibition comparing to acetone, Km for both extracts is 0.39 mM. Na+/CO32- ions induce esterase activity, Na+/CO3-2 ions are not competitive with the inhibitory effects of ethanol. Infect, inhibition of ethanol on gill esterase activity appears to be competitive which is understood by addition of Na+/CO3-2 Since carbonate ions and p-nitrophenyl acetate act on two different catalytic site of this enzyme, both have formed a cooperative effect. It is clear that carbonic anhydrase activity has not been affected till pH:7.4. Although pH above 8.0 enzyme activity has seem to increase, this increase is due to kinetic reaction between substrate (p-nitropheny acetate) and buffer at pH:8-11.5. So. the esteraseactivity of this enzyme at this high pH, can not be evaluated. for the esterase activity of this enzyme. The optimal and unaffected pH for the substrate is 7 for the esterase activity of carbonic anhydrase. "Esterase" activity could be useful for determining "anhydrase" activity of Carbonic anhydrase enzyme of Van lake fish. Stimulation of esterase activity of carbonic anhydrase has been shown by addition of CO32- ions. Carbonic anhydrase present in gill and liver of Van Lake fish has a significant regulatory role for internal pH balance.