Browsing by Author "Eray, Nese"

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Determination of Total Phenolic and Flavonoid Contents of Some Medicinal Plants Growing in Caldiran Province of Van
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2020) Irtem Kartal, Deniz; Eray, Nese; Ozturk, Fazli
The aim of the study was to determine the total phenolic (TPC) and flavonoid (TF) contents of some medicinal plants grown in Caldiran/Van that are frequently consumed by people and to reveal possible differences in the water extracts obtained from different organs. Alcea pallida (Willd.) Waldst. &. Kit. Rumex scutatus L., Mentha longifolia (L.) L., Erigeron acris L., Achillea vermicularis Trin. were collected from Caldiran/Van. TPC and TF contents of the extracts were measured spectrophotometrically. The highest TPC and TF were obtained from the seed extract of E. acris. Stem extracts of the same plant and the stem and seed extracts of M. longifolia were also the samples carrying high phenolic and flavonoids. As a result, it was shown that the flower and seed extracts of five different plants were more efficient than the stem extracts. Biochemical profiles of E. acris and M. longifolia showed that these plants can be used for medicinal purposes and both seed and stem parts contain notably high levels of phenolic and flavonoids.
Effect of Isgin (Rheum Ribes L.) on Biochemical Parameters, Antioxidant Activity and Dna Damage in Rats With Obesity Induced With High-Calorie Diet
(Taylor & Francis Ltd, 2023) Bati, Bedia; Celik, Ismail; Turan, Abdullah; Eray, Nese; Alkan, Elif Ebru; Zirek, Ali Kemal
The present study was designed to investigate the effects ofRheum ribesL. plant root extracts on DNA damage, biochemical and antioxidant parameters in rats with experimental obesity induced with a high-calorie diet. The study groups were divided as "normal control(NC)", "obese control(OC)", "obese +Rheum ribes(OR1)(200 mg/kg)" and "obese +Rheum ribes(OR2)(400 mg/kg)". At the end of the application, rats were sacrificed and blood and tissue samples were obtained. According to the results obtained, the marker of DNA damage in tissues of 8-OHdG was determined to be significantly reduced in brain tissue of the OR1 and OR2 groups compared to the NC group. However, fluctuations were identified in the MDA activity, antioxidant defense system elements and serum biomarkers in tissues. In conclusion,Rheum ribesplant root extract ensured improvements in DNA damage in brain tissues and MDA levels and showed positive effects on antioxidant parameter activities in different tissues.
The Effects of Abiotic Stressors and Signal Molecules on Phenolic Composition and Antioxidant Activities of in Vitro Regenerated Hypericum Perforatum (St. John's Wort)
(Elsevier, 2020) Eray, Nese; Dalar, Abdullah; Turker, Musa
Hypericum perforatum L. is a pharmaceutically and industrially significant natural source due to its rich phenolic content and antioxidant potential. This study aimed to establish an optimized development protocol through plant tissue culture methods and evaluate the effects of abiotic stressors and signal molecules on phenolic composition and antioxidant responses. Methanol based extracts prepared from lyophilized powders of plant samples obtained from nature and aseptic seedlings, and calluses obtained from abiotic stress factors and signal molecules were analysed for phenolic composition using HPLC-MS/MS and antioxidant responses through complementary methods (FCR, FRAP and ORAC). Experimental analysis revealed the use of B5 and SH media for in vitro plant regeneration as the most proper chemical environments for the development and standardization of Hypericum perforatum. Chromatographic findings showed that quercetin, pseudohypericin and partially chlorogenic acid compounds as key phenolic compounds of calluses and plant samples of Hypericum perforatum. The analyses deduced that UV and sucrose increased the level of chlorogenic acid, while ascorbic acid increased the level of quercetin and drought applications increased the concentration of pseudohypericin compared to the control. Rutin compound was only produced in pot samples, which indicates a significant response to the drought stress. Additionally, it was found that B5, SH, and MS media containing of 2 mg/L NAA+1 mg BA with elicitors of 50 mu mol of ascorbic acid and 6% sucrose and a UV stress of 5 (or 10) min-24 h harvesting might be a proper protocol for the optimization and standardization of Hypericum perforatum L. Findings obtained within this study revealed novel contributions for optimization, standardization and production of antioxidative phenolic compounds of Hypericum perforatum plant samples for pharmaceutical and industrial utilization. (C) 2020 SAAB. Published by Elsevier B.V. All rights reserved.
Total Nitrogen Content of Cucumber (Cucumis Sativus L.) Calluses
(Elsevier Science Bv, 2016) Turker, Musa; Eray, Nese; Gormez, Gul