Browsing by Author "Erdem, Sinem Aslan"

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Effects of Arbutin on Fatty Acid Levels of Erythrocyte and Serum in Wistar Albino Rats Treated With Potassium Bromate
(Assoc Pharmaceutical Teachers india, 2022) Akkoyun, H. Turan; Aydin, Sevinc; Akkoyun, Mahire Bayramoglu; Bengu, Aydin Sukru; Ekin, Suat; Erdem, Sinem Aslan
Background: In the presented study; the Effects of Arbutin (ARB) on the Rat Erythrocyte and serum fatty acid profile which is exposed to potassium bromate (KBrO3) were investigated. Materials and Methods: In this study, 32 Wistar albino rats weighing 250-300 g were used divided into 4 groups. Groups 1: control, group 2: KBrO3 (single dose 100 mg / kg gavage), group 3: ARB (50 mg / kg / day (ip) for 5 days), group 4: KBrO3 + ARB. At the end of the 5(th) day, alteration of fatty acid profile in erythrocyte and serum of rats in all groups was examined. Results: Rat serum essential fatty acid; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), erythrocyte major fatty acids; palmitic acid (C16:0), myristic acid (C14:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), arachidic acid (C20:0), eicosenoic acid (C20:1), and lignoceric acid (C24:0). In addition, in our studied serum and erythrocytes; Total monounsaturated fatty acids (MUFA) varied between 8.91 +/- 0.53- 11.71 +/- 2.55 and 33.71 +/- 2.12- 37.11 +/- 2.12, respectively. It was determined that total polyunsaturated fatty acids (PUFA) varied between 5.90 +/- 1.29- 9.96 +/- 1.18 and 14.72 +/- 3.66- 22.13 +/- 4.82, respectively. Conclusion: In our study, alterations in fatty acid contents were observed, and results suggesting that arbutine affects the enzymes involved in Fatty acid metabolism and has an effect on fatty acid amounts.
The Protective Effect of Arbutin Against Potassium Bromate-Induced Oxidative Damage in the Rat Brain
(Wiley, 2023) Akkoyun, H. Turan; Uyar, Ahmet; Akkoyun, Mahire Bayramoglu; Bengu, Aydin Sukru; Melek, Sule; Karagozoglu, Fatma; Erdem, Sinem Aslan
This study aimed to investigate the protective effects of arbutin (ARB) against brain injury induced in rats with potassium bromate (KBrO3). The rats were divided into four groups as Group 1: Control (0.9% NaCl ml/kg/day p.), Group 2: KBrO3 (100 mg/kg (gavage), Group 3: ARB (50 mg/kg/day p.), and Group 4: KBrO3 + ARB (100 mg/kg (gavage) + 50 mg/kg/day p.). At the end of the fifth day of the study, the rats in all groups were killed, and their brain tissues were collected. In the collected brain tissues, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels were measured, and routine histopathological examinations were made. The MDA levels in the group that was exposed to KBrO3 were significantly higher than those in the control group (p < 0.001). In comparison to the KBrO3 group, the MDA levels in the KBrO3 + ARB group were significantly lower (p < 0.001). It was observed that SOD and CAT enzyme activity levels were significantly lower in the KBrO3 group compared to the control group (p < 0.001), while these levels were significantly higher in the KBrO3 + ARB group than in the KBrO3 group (p < 0.001). Additionally, the group that was subjected to KBrO3 toxicity, as well as ARB administration, had much lower levels of histopathologic signs than the group that was subjected to KBrO3 toxicity only. Consequently, it was found that KBrO3 exposure led to injury in the brain tissues of the rats, and using ARB was effective in preventing this injury.
Tyrosinase and Cholinesterase Inhibitory Potential and Flavonoid Characterization of Viola Odorata L. (Sweet Violet)
(Wiley, 2015) Orhan, Ilkay Erdogan; Senol, Fatma Sezer; Erdem, Sinem Aslan; Tatli, I. Irem; Kartal, Murat; Alp, Sevket
Inhibitory potential of the dichloromethane, ethyl acetate, ethanol, and aqueous extracts of Viola odorata L. (VO) was investigated against tyrosinase (TYR) and cholinesterases by microplate assays. The antioxidant activity was tested using six in vitro assays. Only the ethanol extract inhibited TYR (80.23 +/- 0.87% at 100 mu gmL(-1)), whereas none of them were able to inhibit cholinesterases. The extracts were more able to scavenge NO radical (31.98 +/- 0.53-56.68 +/- 1.10%) than other radicals tested, and displayed low to moderate activity in the rest of the assays. HPLC analysis revealed that the aqueous extract of VO contained a substantial amount of vitexin (18.81 +/- 0.047mgg(-1) extract), while the ethanol extract also possessed rutin (1.31 +/- 0.013mgg(-1) extract) and vitexin (4.65 +/- 0.103mgg(-1) extract). Furthermore, three flavonoids (rutin, isovitexin, and kaempferol-6-glucoside) were isolated from the ethanol extract. This is the first report on TYR inhibitory activity of VO as well as presence of vitexin and isovitexin in this species. Copyright (c) 2015 John Wiley & Sons, Ltd.