Browsing by Author "Erdenlig, Sevil"

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Investigation of Brucella Spp. Presence of Amphibians Collected From Some Regions in Türkiye
(2024) Akar, Kadir; Yücetepe, Ayfer Güllü; Öz, Gülseren Yıldız; Baklan, Emin Ayhan; Eroglu, Buket; Atıl, Eray; Erdenlig, Sevil
Brucellosis is a zoonotic disease that causes economic losses in many countries worldwide, especially in livestock. Firstly, the African bullfrog and then B. inopinata and B. microti-like strains have been identified in various frog species worldwide. This study aimed to determine the presence of Brucella in amphibian frogs for the first time in Türkiye by bacteriological and molecular methods. Internal organ samples (spleen, liver, lung, kidney, etc.) of 150 frogs from different regions of Türkiye (Edirne/Ipsala (53 pieces), Adıyaman/Kâhta (97 pieces)) were used for the isolation of Brucella spp. As a result of Brucella genus-specific PCR (Polymerase Chain Reaction) and Multiplex PCR methods performed with these extracts, no positivity was detected in the frog samples taken from Edirne province, while in 4 of the frog samples taken from Adıyaman province, bands of approximately 250, 600, 700, 1000, 1500 and 3000 bp were observed in Multiplex PCR. Sphingomonas paucimobilis (S. paucimobilis) was identified by analysing the culture obtained from these samples with VITEK®2:Healtcare. As a result, the presence of Brucella spp. could not be detected both bacteriologically and molecularly in the study samples. However, observing similar multiple bands in multiplex PCR suggested that this bacterium and Brucella species are phylogenetically close. It was determined that S. paucimobilis, which belongs to the same class as Brucella species under the Alphaproteobacteria, may be dangerous for people who work on farms where frogs are raised for human consumption. This is because the bacteria can cause opportunistic infections, particularly in immunocompromised humans. Therefore, it may be imperative to take the appropriate precautions.
A Serological Diagnostic Survey for Brucella Canis Infection in Turkish Patients With Brucellosis-Like Symptoms
(Natl inst infectious Diseases, 2011) Sayan, Murat; Erdenlig, Sevil; Stack, Judy; Kilic, Selcuk; Guducuoglu, Huseyin; Aksoy, Yavuz; Etiler, Nilay
The incidence of Brucella canis infection in humans is unknown in Turkey. In this study, we investigated the prevalence of B. canis infection in human sera obtained from six regions in Turkey and comparatively evaluated the results obtained by agglutination-based techniques using standardized antigens made from B. canis. The patients (n = 1,746) presented with clinical symptoms that were similar to those of brucellosis. All patients who tested negative in the Rose Bengal test for the smooth Brucella strains (abortus, melitensis, and suis) were screened for evidence of B. canis infection using the rapid slide agglutination test (RSAT), the microagglutination test (MAT), and the 2-mercaptoethanol RSAT test (2ME-RSAT). Of the samples tested, 157(8.9%), 68(3.8%), and 66(3.7%) were positive for B. canis, as determined by RSAT, MAT, and 2ME-RSAT, respectively. The diagnostic sensitivity, specificity, positive predictive value, and negative predictive value of RSAT were 100%, 94.6%, 42%, and 100%, respectively, and of MAT were 100%, 99.9%, 970, 70 and 100%, respectively. We recommend the routine use of MAT and 2ME-RSAT to check the sera of all patients with symptoms of brucellosis who are negative for brucellosis using a smooth Brucella antigen.