Browsing by Author "Erdogan, E"

Now showing 1 - 7 of 7

The Autonomic Innervation of the Testicular Parenchyma
(Scandinavian Federation Laboratory Animal Science, 2003) Erdogan, E; Aydinlioglu, A; Ragbetli, MC; Özbek, H; Göçer, F
The specific roles and direct involvements of autonomic innervations on the spermatogenic process are poorly understood. The aim of this study was to investigate stereologically the relative importance of sympathetic innervations in testicular parenchyma rats in chemically sympathectomized with guanethidine. Treated animals (n=10) were injected intraperitoneally with guanethidine at doses of 10mg/kg/day for 15 days while control animals (n= 5) received an equivalent volume of saline. After routine histological procedures, 5mum thick sections of the testes were selected for examination. Organ volumes were estimated using the Cavalieri Principle of volume measurement by means of consecutive serial sections, using "J Images" software in a computer. At least 10 seminiferous tubules were selected randomly and measured per cross section for evaluation of epithelial heights, luminal diameter and total seminiferous tubule diameter. Testicular volumes and seminiferous tubule measurements of treated animals were found to be affected by the chemical sympathectomy with guanethidine with a a statistically significant difference between experimental and control group (p<0.01). Our findings indicate that chemical sympathectomy with-short term low dose guanethidine might display morphometric changes in the rat testis which indicate the presence of autonomic innervation of its parenchyma.
The Effect of Ginkgo Extract Egb761 in Cisplatin-Induced Peripheral Neuropathy in Mice
(Academic Press inc Elsevier Science, 2004) Öztürk, G; Anlar, Ö; Erdogan, E; Kösem, M; Özbek, H; Türker, A
Neuroprotective effect of Ginkgo biloba extract EGb761 in cisplatin (cis-diamminedi-chloroplatinum, or CDDP)-induced peripheral neuropathy was investigated. Swiss albino mice were treated with CDDP, 2 mg/kg ip twice a week for nine times. One group of the animals also received EGb761 in the drinking water at an estimated dosage of 100 mg/kg per day. Two other groups received vehicle (control) or EGb761 only. Development of neuropathy was evaluated with changes in sensory nerve conduction velocity (NCV). Following the treatments, dorsal root ganglia (DRGs) were microscopically examined and some were cultured for 3 days. EGb761 proved effective in preventing the reduction in NCV (P < 0.0001) caused by CDDP. CDDP caused a decrease in the number of migrating cells (P < 0.01) and in the length of outgrowing axons (P < 0.01) while EGb761 treatment prevented the latter. CDDP led to smaller nuclear and somatic sizes in neurons (P < 0.01), while with EGb761 co-administration, both were close to control values. Animals having EGb761 only had similar results with controls. In conclusion, EGb761 was found to be effective in preventing some functional and morphological deteriorations in CDDP-induced peripheral neuropathy. (C) 2004 Elsevier Inc. All rights reserved.
Effect of Leukemia Inhibitory Factor in Experimental Cisplatin Neuropathy in Mice
(Academic Press Ltd- Elsevier Science Ltd, 2005) Öztürk, G; Erdogan, E; Anlar, Ö; Kösem, M; Taspinar, M
In this study, the effect of leukemia inhibitory factor (LIF) on cisplatin (CDDP)-induced neuropathy was evaluated. Mice were treated with CDDP, 2 mg/kg i.p. twice a week nine times. During the last week some of the mice were also injected with LIF, 2 mug/kg s.c. every other day for a total of four injections. Development of neuropathy was evaluated with changes in tail flick latency and sensory nerve conduction velocity (NCV). At the end of the treatment period dorsal root ganglia (DRG) were microscopically examined. Some of the DRGs were explanted into extracellular matrix, covered with culture medium and incubated for 3 days. During and at the end of the incubation, cellular migration and axonal outgrowth from the DRGs were quantified. LIF proved effective in reversing the increase in tail flick latency (p < 0.05) and improving the reduction in NCV induced by CDDP. CDDP led to smaller nuclear and somatic size in neurons, while with LIF, the latter was restored to control values (p<0.01). No apoptotic nucleus was observed among DRG neurons while very few and moderate numbers detected among satellite and Schwann cells, respectively. With LIF, none of the cells had apoptosis. CDDP caused a decrease in the number of migrating cells and in the length of outgrowing axons while LIF treatment restored both capacities (p < 0.05) In conclusion, in CDDP-induced neuropathy, LIF was found to be effective in correcting some functional and morphological deteriorations related with major involvement of Schwann cells. (C) 2004 Elsevier Ltd. All rights reserved.
The Evaluation of Fetal Hepatocyte Transplantation for Metabolic Support
(indian veterinary Journal, 2005) Erdogan, E; Soylu, R; Duman, S
Severe liver diseases threat the life and have a high mortality rate (Dixit, 1993). The most effective therapy is orthotopic liver transplantation (Hillan et al., 1989). Especially in the childhood period, donor scarcity is the major problem (Vacanti, 1988). As an alternative approach., hepatocyte transplantation is being widely studied (Aiken et al., 1990). Fetal liver cell transplantation, with many advantage, have been used and preferred as bridge transplantation (Dixit, et al., 1993) as these cells give more time and a choice for regeneration (Demetriou et al., 1986). In this study, we evaluated the metabolic and survival efficiency of intrasplenic fetal hepatocyte transplantation in the surgically induced acute hepatic failure in rats.
Morphological Quantitative Changes in the Number of Lymphocytes, Macrophages and Plasma Cells in the Uterus and Lymph Nodes of Rats Exposed To the Systemic Administration of Bcg
(Tohoku Univ Medical Press, 2003) Kanter, M; Gul, A; Meral, I; Koc, A; Ilhan, M; Erdogan, E
KANTER, M., GUL, A., MERAL, I., Koc, A., ILHAN, M. and ERDOGAN, E. Morphological Quantitative Changes in the Number of Lymphocytes, Macrophages and Plasma Cells in the Uterus and Lymph Nodes of Rats Exposed to the Systemic Administration of BCG. Tohoku J. Exp. Med., 2003, 199 (4), 219-228 - This study was designed to investigate the effect of systemic administration of BCG on the morphological quantitative changes in the number of lymphocytes, macrophages and plasma cells in the uterus and lymph nodes of rats. Thirty female virgin Wistar Albino rats, aging 6 months and weighing between 200-250 g, were assigned to the two experimental groups; BCG treated and controls (n = 15). BCG group received 0.1 ml BCG in tail skin and control group received 0.1 ml saline at the same place. Two weeks after injections, rats in both groups were anesthesized with a high dose of ether and decapitated. Uterus and ileocecal lymph nodes were processed to determine alpha napthyl acid esterase (ANAE)-positive T lymphocytes and macrophages. The plasma cells were stained with the methyl green-pyronin method. It was found that the numbers of T lymphocytes, macrophages and plasma cells in the uterus and the ileocecal lymph nodes of BCG treated group significantly increased indicating the presence of an immune response to the systemic BCG administration. It was concluded that the systemic administration of BCG increases humoral and cellular immunity in endometrium, myometrium and regional lymph nodes. The immune deficiency system plays an important role in the pathogenesis of endometriosis. Therefore, the endometriosis might be prevented by using periodical administration of BCG. However, further experimental and clinical studies associated with these issue are required.
Multidimensional Long-Term Time-Lapse Microscopy of in Vitro Peripheral Nerve Regeneration
(Wiley, 2004) Öztürk, G; Erdogan, E
In order to test the effectiveness of a new advanced time-lapse microscopy imaging and image processing and analysis system, and to do quantitative and qualitative temporal analyses of in vitro peripheral nerve regeneration, long-term time-lapse imaging of cultures of mouse dorsal root ganglia (DRGs) was performed. DRGs were placed in a Petri dish, covered with collagen gel, their attached peripheral nerves were cut in the middle, creating a gap, and the dish was filled with culture medium. Six preparations were kept on the time-lapse imaging system, which provides a suitable incubation environment and enables to capture images from multiple coordinates at x,y,z axes at desired time intervals for 13 days. In general, the time-lapse imaging system proved quite stable and efficient, although some improvements are certainly required. Two main components of peripheral nerve regeneration, outgrowth of axons and activities of resident cells, were examined. Axons started to grow during the first hour of incubation with a 16.5 mum/h rate and showed the slowest rates (0.7 mum/h) on days 8 and 9, after which they resumed higher speeds again. The first cell came out of the proximal end of the cut nerve on the second day and it was a Schwann cell (SC), which was the prominent cell type in the preparations throughout the experiment. SCs were higher in number (83.15% of all cells) but slower in migration (3.4 vs. 7.3 mum/h, P < 0.001) than other cells. Other observed characteristics of axonal outgrowth and cellular activity and interactions between axons and the cells are discussed. (C) 2004 Wiley-Liss, Inc.
The Relationships of Dog Hippocampus To Sex and Paw Preference
(Taylor & Francis Ltd, 2006) Aydinlioglu, A; Arslan, K; Cengiz, N; Ragbetli, M; Erdogan, E
Previous studies have described paw preference and asymmetry in dog, brains. Electrical activity of the dorsal hippocampus also indicated the existence of hippocampal asymmetry in dogs. In the present study, the possible paw and sex-related asymmetries and right-left differences in dog hippocampus were investigated. The hippocampus was dissected and weighed. Each hippocampus was cut into slices by the slicing apparatus placed horizontally on the tissues. The volumetric measurements were performed using the formula modified from the Cavalieri principle. The present study indicated the significant sex and paw differences and no right-left asymmetry in dog hippocampi. The morphological asymmetries in normal subjects might be related to functional hippocampal asymmetries in memory or in cognitive skills.