Browsing by Author "Erdogan, E."

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Acute Myotoxic Effects of by Infusion of Prilocaine and Lidocaine in Rats
(Medwell online, 2009) Ragbetli, M. C.; Yalama, M.; Erdogan, E.; Cengiz, N.; Kati, I.; Ragbetli, C.
We assumed to examine the acute myotoxic effects of infusion of the local anesthetic lidocaine and prilocaine on the gluteus maximus muscle after continuous peripheral nerve blockade in rats. Eight adult female Sprague-Dawley average weighing 150-200 g rats were used in this study. Firstly, all of the animals were anesthetized with ketamine (50 mg kg(-1)) for 6 h. Then, prilocaine (right) and lidocaine (left) were used in equal volume (5 mg mL(-1)) for continuous peripheral nerve blockades of the posterior extremity at a rate of 0.3 mL h(-1) for a total period of 6 h in 5 animals. The remaining 3 animals as control group were treated with physiological saline on both sides at 0.3 mL h(-1) for a total period of 6 h. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. For routine histological observation, the infusion area was dissected and tissue samples including peripheral muscle were taken. Method histological hazards as myotoxicite were not observed in skeletal muscle tissue after the infusion of prilocaine and lidocaine. There were no complications cases of local anesthesia. Prilocaine and lidocaine as local anesthetics might be applied safely by infusion.
Determination of Hormones Inducing Oocyte Maturation in Chalcalburnus Tarichi (Pallas, 1811)
(Springer, 2008) Unal, G.; Erdogan, E.; Oguz, A. R.; Kaptaner, B.; Kankaya, E.; Elp, M.
Chalcalburnus tarichi is an endemic cyprinid species living in the Lake Van basin, in eastern Anatolia, Turkey. The present study was undertaken to determine which hormones induce oocyte maturation in C. tarichi. The levels of 17 alpha,20 beta,21-trihydroxyprogesterone (20 beta-S), progesterone (P), 17 alpha-hydroxyprogesterone (17 alpha-HOP), 11-deoxycortisol (11-DOC), and 17 alpha-hydroxy-20 beta-dihydroprogesterone (17,20 beta-P) were measured in fish caught from Lake Van and the Karasu River, and injected with human chorionic hormone (hCG) (1,000 and 1,500 IU/kg). Oocytes of fish caught from the lake were also incubated in vitro with different doses (50, 200, and 1,000 ng/ml) of 20 beta-S, 17 alpha-HOP, 11-DOC, and 17,20 beta-P. 11-DOC was found to be the most effective hormone among those measured for inducing oocyte maturation in vivo and in vitro. 17,20 beta-P could not be determined in the plasma of any fish in vivo (P < 0.05). 1,000 IU/kg dose of hCG given by injection caused a statistically significant increase in all plasma hormone levels (P < 0.05). It was found that there was a significant decrease in the P level only at 1,500 IU/kg dose of hCG injected (P < 0.05), while the level of other hormones increased at this dose (P < 0.05). It was also determined that all the hormones were effective in germinal vesicle breakdown (GVBD) in in vitro oocyte culture (P < 0.05). However, 11-DOC was found to be the most effective hormone in GVBD at a dose of 200 ng/ml (70% GVBD). In conclusion, 11-DOC synthesized during final oocyte maturation in C. tarichi was found to be a potent inducer of GVBD, which shows that 11-DOC may be described as an oocyte maturation steroid in this species.
Differential Analysis of Effect of High Glucose Level in the Development of Neuropathy in a Tissue Culture Model of Diabetes Mellitus: Role of Hyperosmolality
(Johann Ambrosius Barth verlag Medizinverlage Heidelberg Gmbh, 2008) Ozturk, C.; Erdogan, E.; Oesztuerk, M.; Cengiz, N.; Him, A.
To analyse the contributions of metabolic toxicity of high glucose level and accompanying hyperosmolality to the development of diabetic neuropathy, mouse dorsal root ganglion (DRG) cultures were used. DRGs from postnatal day 7 mice were embedded in collagen gel and incubated in RPMI 1640 Culture medium with increasing concentrations of glucose or equimolar amounts of mannitol which would create similar osmolalities. Outgrowth of axons from the peripheral nerve attached to DRG and migration of cells into the gel were quantified. The extent of cell death, apoptosis and mitosis among the migrating cells and apoptosis among DRG neurons following exposure to high glucose or mannitol were also evaluated. The growth of axons was almost equally affected by increasing concentrations Of glucose or mannitol up to 395 mOsm/kg H2O. Number of migrating cells was close to control values with mannitol between 340-395 mOsm/kg H2O while high concentrations of glucose always decreased it. Exposure to high glucose or mannitol led to increased proportions of dead and apoptotic migrating cells and apoptotic DRG neurons. Mitotic activity was also negatively affected by high glucose or mannitol. While glucose proved significantly more detrimental to migrating cells than mannitol in the latter tests, the extent of apoptosis was similar among DRG neurons in both conditions. In conclusion, the contribution of hyperosmolality to the development of neuropathy in high glucose condition appears to be quite significant. The peripheral nerve cells and neurons, however, are differentially affected by hyperosmolality and metabolic toxicity of high glucose.
Elastic Characteristics of the Aorta in Patients With a New Diagnosis of Metabolic Syndrome
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Çakmak, E.Ö.; Findikcioglu, U.; Erdogan, E.; Zencirci, E.; Karagoz, A.; Celik, F.S.E.; Soylu, Ö.
Metabolic syndrome (MS) is a known risk factor of cardiovascular disease. However, it is not identified whether MS made alterations in the elasticity of the aorta in the early period before significant atherosclerosis occurred. The purpose of the study was to evaluate aortic elastic properties of patients who were newly diagnosed with MS. The research was performed among 100 patients of newly diagnosed MS (49 males; mean age 46 ± 9 years) with normal sinus rhythm, and 55 cases without MS (29 males; mean age 45 ± 9 years), matched by age. All participants underwent comprehensive physical and cardiological examination, biochemical examination, anthropological measurement and echocardiography. Aortic diameter change was significantly lower in MS group compared to control group (0.014 ± 0.04 vs 0.25 ± 0.1, p <0.01), aortic stiffness was significantly higher in the MS group (10.65 ± 4.52 vs 5.7 ± 2.42, p<0.01) compared to the age-matched control group. Multiple regression analysis shows that there is an independent relationship with each of the age, body mass index, HDL cholesterol and systolic blood pressure. Aortic stiffness index was higher in newly diagnosed MS patients compared to the control group. The vascular system can be affected even without diabetes, hypertension, hyperlipidemia, and coronary artery disease, which is excluded by history and noninvasive evaluation. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Short-Term Effect of Percutaneous Recanalization of Chronic Total Occlusions on Qt Dispersion and Heart Rate Variability Parameters
(2013) Erdogan, E.; Akkaya, M.; Bacaksiz, A.; Tasal, A.; Sönmez, O.; Asoglu, E.; Göktekin, O.
Background: QT dispersion (QTd), which is a measure of inhomogeneity of myocardial repolarization, increases following impaired myocardial perfusion. Its prolongation may provide a suitable substrate for life-threatening ventricular arrhythmias. We investigated the changes in QTd and heart rate variability (HRV) parameters after successful coronary artery revascularization in a patient with chronic total occlusions (CTO). Material/Methods: This study included 139 successfully revascularized CTO patients (118 men, 21 women, mean age 58.3±9.6 years). QTd was measured from a 12-lead electrocardiogram and was defined as the difference between maximum and minimum QT interval. HRV analyses of all subjects were obtained. Frequency domain (LF: HF) and time domain (SDNN, pNN50, and rMSSD) parameters were analyzed. QT intervals were also corrected for heart rate using Bazett's formula, and the corrected QT interval dispersion (QTcd) was then calculated. All measurements were made before and after percutaneous coronary intervention (PCI). Results: Both QTd and QTcd showed significant improvement following successful revascularization of CTO (55.83±14.79 to 38.87±11.69; p<0.001 and 61.02±16.28 to 42.92±13.41; p<0.001). The revascularization of LAD (n=38), Cx (n=28) and RCA (n=73) resulted in decrease in HRV indices, including SDDN, rMSSD, and pNN50, but none of the variables reached statistical significance. Conclusions: Successful revascularization of CTO may result in improvement in regional heterogeneity of myocardial repolarization, evidenced as decreased QTcd after the PCI. The revascularization in CTO lesions does not seem to have a significant impact on HRV. © Med Sci Monit, 2013.
Successful Surgical Treatment of a Rare Case of Acute Isolated Right Ventricle Wall Rupture Caused by Distal Circumflex Coronary Artery Occlusion
(Sociedade Brasileira de Cirurgia Cardiovascular, 2022) Erdogan, E.; Kilic, Y.; Tuner, H.; Sahinalp, S.; Ramasamy, A.
We describe a rare case of isolated right ventricular inferior free-wall rupture and cardiogenic shock caused by occlusion of the distal left circumflex coronary artery. Our case highlights the central role of transthoracic echocardiography in identifying unexpected conditions that can guide management – in our case involving early surgical intervention, thus leading to favourable patient outcomes. © 2022, Sociedade Brasileira de Cirurgia Cardiovascular. All rights reserved.
Superficial Femoral Artery Mirror Lesions; Risk Factors, Diagnosis, and Clinical Presentation
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Erdogan, E.; Cakmak, E.O.; İmanov, E.; Tosun, A.; Tekatlı, A.F.; Geçmen, Ç.; Koksal, C.
Two-sided atherosclerotic involvement of peripheral arteries is common in clinical practice; however, the data about it in the literature is limited. This study aimed to investigate the frequency, clinical characteristics, and predictors of bilateral symmetric total superficial femoral artery (SFA) occlusions (mirror lesions). Between January 2015 and April 2020, 167 patients with symptomatic total SFA occlusions were retrospectively analyzed. Unilateral and bilateral SFA occlusions were determined, and the risk factors and clinical presentation were investigated between the two groups. Among 167 patients, 95 (57%) had bilateral SFA lesions, and 30 (18%) presented with critical limb ischemia (CLI). In the bilateral SFA occlusion group, male sex was dominant (p=0.002), and peripheral artery disease (PAD) history was higher (p=0.030). Additional iliac lesions (p = 0.003) and below-knee lesions (p <0.001) were more common in the bilateral SFA occlusion group. The sensitivity of Doppler ultrasonography in detecting SFA occlusions was 83.9%. In multivariate logistic regression analysis, younger age (OR; 0.95 CI; 0.91-0.99 p = 0.030) and history of PAD (OR; 2.08 CI; 1.02-4, 24 p = 0.040) were identified as independent markers for predicting mirror SFA occlusions Our study demonstrates that the mirror lesion pattern is common in patients with symptomatic SFA total occlusion. Thus, the non-index leg evaluation should be recommended. The younger the age, and PAD history were the predictors of mirror total SFA occlusions. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Two Distinct Types of Dying Back Axonal Degeneration in Vitro
(Wiley, 2013) Ozturk, G.; Cengiz, N.; Erdogan, E.; Him, A.; Oguz, E. K.; Yenidunya, E.; Aysit, N.
G. ozturk, N. Cengiz, E. Erdoan, A. Him, E. K. Ouz, E. Yenidunya and N. Ayit (2013) Neuropathology and Applied Neurobiology39, 362376 Two distinct types of dying back axonal degeneration in vitro Aims: In many neurodegenerative diseases and following traumas, dying back degeneration is a common phenomenon that aggravates the pathology and may eventually lead to death of the affected neurone. We aimed to investigate the mechanism of dying back degeneration with an in vitro axonal injury model. Methods: We cultured adult mouse dorsal root ganglion neurones and with a precise laser beam, cut the axons they extended. Preparations were imaged continuously and images were analysed to describe and quantify ensuing events. Potential contributions of calpains and caspases to the degeneration were explored using specific inhibitors and immunohistochemistry. In vivo implications of the results were sought in nerve sections after sciatic nerve cut. Results: The proximal part of the transected axons went under basically two types of dying back degeneration, fragmentation and retraction. In fragmentation the cytoplasm became condensed and with concomitant axial collapse the axon disintegrated into small pieces. In retraction, the severed axon was pulled back to the soma in an organized manner. We demonstrated that fragmentation was associated with a high risk of cell death, while survival rate with retraction was as high as those of uninjured neurones. Regeneration of transected axon was more likely after retraction than following fragmentation. Activities of caspase-3 and calpains but not of caspase-6 were found linked with retraction and regeneration but not with the fragmentation. Conclusions: This study describes two quite distinct types of dying back degeneration that lead an injured neurone to quite different fates.