Browsing by Author "Erdogan, Ender"

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Analgesic and Hepatotoxic Effects of Ononis Spinosa
(Wiley, 2006) Yilmaz, Betul Sever; Ozbek, Hanefi; Citoglu, Gulcin Saltan; Ugras, Serdar; Bayram, Irfan; Erdogan, Ender
The present study investigated the analgesic and hepatoprotective activities of a water extract of Ononis spinosa L. (OS) in mice. Analgesic activity was based on the pain thresholds measured with the tail-flick test before administration at 30, 90 and 150 min. The results were analysed with one-way variance analysis. The extract of Ononis spinosa showed analgesic activity equivalent to aspirin at 30 and 90 min and even higher than aspirin with the 50 mg/kg dose. At a dose of 100 mg/kg OS showed an analgesic effect equivalent to aspirin at all time points. The hepatoprotective influence of OS on carbon tetrachloride (CCl4)-induced acute liver toxicity was also studied. The extract had no significant effect on the increased levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and bilirubin in CCI4 treated animals (p > 0.05). Thus, the results reveal that the extract of OS had no hepatoprotective effect on CCI4-induced acute liver toxicity. Copyright (c) 2006 John Wiley & Sons, Ltd.
Are Nigella Sativa and Urtica Dioica Fixed Oils Effective on Carbon Tetrachloride-Induced Liver Injury Model in Rats
(Studium Press Llc, 2008) Ozbek, Hanefi; Ozturk, Mustafa; Erdogan, Ender; Kosem, Mustafa
Effectiveness of Nigella sativa and Urtica dioica. in prevention of carbon tetrachloride-induced liver injury were investigated in this study. Sprague-Dawley rats were given carbon tetrachloride dissolved in olive oil intraperitoneally three times a week for 11 weeks. After liver fibrosis was developed, carbon tetrachloride was discontinued and rats were randomized in four groups. They were treated with Nigella sativa fixed oil (2 ml/kg), Urtica dioica fixed oil (2 ml/kg) or Nigella sativa (2 ml/kg) and Urtica dioica (2 ml/kg) per orally for four weeks. The control group was untreated. The animals were sacrificed and specimens were taken for biochemical and histopathological study at the end. Cessation of carbon tetrachloride allowed for partial recovery of liver tissue by autoregeneration in control group. Liver fibrosis was further progressed in all three treatment groups and resulted in cirrhosis in some animals. In Urtica dioica group alanine aminotransferase level was significantly higher compared to other groups. Serum indirect bilirubin levels were higher in Urtica dioica and Nigella sativa groups compared to control and Nigella sativa+Urtica dioica groups. Present study indicate that fixed oils of Nigella sativa, Urtica dioica and Nigella sativa +Urtica dioica did not show a therapeutic effect in experimental liver fibrosis in rats.
Comparison of Techniques for Long-Term Storage of Fat Grafts: an Experimental Study
(Lippincott Williams & Wilkins, 2006) Atik, Bekir; Ozturk, Gurkan; Erdogan, Ender; Tan, Onder
Background: Absorption of autologous fat graft in the recipient area necessitates recurrent fat transplantation. Harvesting extra tissue during the first operation and storing it for future use is considered a solution to this problem. Methods: Fat tissue was removed from the inguinal region of 40 Swiss albino mice, which were arranged into four equal groups, and treated as follows: immediately transplanted to the donor animal; dry frozen; immersed in glycerol; or frozen in liquid nitrogen. The grafts that were frozen or immersed in glycerol were stored at -35 degrees C for 6 months and then transplanted to their original donors. Transplantations were performed by injecting the fat under the scalp. Viability of the fat tissue was evaluated with the MTT reduction test before transplantation, and histology of the transplanted tissue was examined at the end of the study. Results: The viability and histology of grafts frozen in liquid nitrogen were similar to those of fresh tissue, whereas with other methods the grafts had a considerable loss of viability during storage that was reflected in the low number of adipocytes and high proportion of vacuolar and fibrotic areas. Conclusion: Freezing fat grafts in liquid nitrogen and storing them at -35 degrees C is an effective way of preserving tissue for future use, with clear superiority over other methods.
Consequences of Neurite Transection in Vitro
(Mary Ann Liebert inc, 2012) Cengiz, Nurettin; Ozturk, Gurkan; Erdogan, Ender; Him, Aydin; Oguz, Elif Kaval
In order to quantify degenerative and regenerative changes and analyze the contribution of multiple factors to the outcome after neurite transection, we cultured adult mouse dorsal root ganglion neurons, and with a precise laser beam, we transected the nerve fibers they extended. Cell preparations were continuously visualized for 24 h with time-lapse microscopy. More distal cuts caused a more elongated field of degeneration, while thicker neurites degenerated faster than thinner ones. Transected neurites degenerated more if the uncut neurites of the same neuron simultaneously degenerated. If any of these uncut processes regenerated, the transected neurites underwent less degeneration. Regeneration of neurites was limited to distal cuts. Unipolar neurons had shorter regeneration than multipolar ones. Branching slowed the regenerative process, while simultaneous degeneration of uncut neurites increased it. Proximal lesions, small neuronal size, and extensive and rapid neurite degeneration were predictive of death of an injured neuron, which typically displayed necrotic rather than apoptotic form. In conclusion, this in vitro model proved useful in unmasking many new aspects and correlates of mechanically-induced neurite injury.
The Effect of Non-Enzymatic Glycation of Extracellular Matrix Proteins on Axonal Regeneration in Vitro
(Springer, 2006) Ozturk, Gurkan; Sekeroglu, Mehmet Ramazan; Erdogan, Ender; Ozturk, Mustafa
Non-enzymatic glycation of peripheral nerve extracellular matrix (ECM) may contribute to the development of diabetic distal sensory neuropathy (DNP). We investigated the relative importance of glycation of collagen types I and IV, laminin and fibronectin in DNP-related impairment in peripheral nerve regeneration. Dorsal root ganglia (DRGs) from young adult mice were embedded in collagen type I modified by 10% substitution with normal or glycated forms of the proteins and incubated for 3 days. Outgrowth of axons and migration of cells into the ECM were quantified. Mean length of growing axons was significantly reduced by glycation of laminin and collagen type IV. The sum of lengths of all axons from each DRG was greatly reduced with glycated laminin, collagen types IV and I. Glycation of fibronectin had no effect on axonal growth. The number of migrating cells was not affected by glycation. We conclude that non-enzymatic glycation of laminin and collagen types IV and I (in decreasing order) impairs peripheral nerve regeneration in vitro.
Effects of Topical Mitomycin and Trimetazidine on Myringosclerosis
(B C decker inc, 2008) Cankaya, Hakan; Kosem, Mustafa; Kiroglu, Faruk; Kiris, Muzaffer; Yuca, Koeksal; Ozturk, Guerkan; Erdogan, Ender
Introduction: Myringosclerosis, one of the most common complications of ventilation tube placement, is a kind of tympanosclerosis and is defined as subepithelial hyalinization of the tympanic membrane. There are two arguments in the development of myringosclerosis: inflammation triggering the development of myringosclerosis and free oxygen radicals released during inflammation causing myringosclerosis. Objective: The aim of the present study was to explore the effects on the development of myringosclerosis of mitomycin, which has anti-inflammatory effects, and trimetazidine, which is believed to inhibit free oxygen radicals when given systemically. Materials and Method: The study was carried out on rabbits. Animals were divided into five groups, with six rabbits in each group: three control groups (paracentesis only, paracentesis + tube placement, and no intervention), a trimetazidine group, and a mitomycin group. Mitomycin (0.4 mg/mL) and trimetazidine (20 mg/mL) were applied topically to the tympanic membrane, and the presence and degree of sclerosis were graded histopathologically after Masson's trichrome staining. Results: In the histopathologic examination, sclerosis that developed in the tympanic membranes of rabbits that had undergone paracentesis or paracentesis plus tube application or received trimetazidine was significantly more extensive than sclerosis in the membranes of unoperated animals and those that had been administered mitomycin. Conclusions: Paracentesis in rabbits, independent of tube placement, causes sclerosis of the tympanic membrane. Results show that topical use of mitomycin, due to its anti-inflammatory effect, had alleviating effects on myringosclerosis, whereas topical trimetazidine did not.
A New Method in Cns (Central Nervous System) in Vitro Cultures in the Mouse: Study of Effectiveness
(Kafkas Univ, veteriner Fakultesi dergisi, 2010) Erdogan, Ender; Ozturk, Gurkan; Ragbetli, Murat Cetin
In this study: to evaluate the effectiveness of collagen coating method, using in the peripheral nervous system cultures. and its involving factors caused from manipulations in central nervous system (CNS) cultures was aimed. Via frontal approach, brains, transected from young Swiss albino mice, were taken into artificial cerebro-spinal fluid immediately and made blocks in agarose gel. With a vibration microtome, 200 pm thickness horizontally live slices were taken in to the dishes filled with culture medium. Tissue sections were analyzed as two groups. In the group 1 (control): fresh slices were evaluated directly. In the group 2: sections were covered with collagen gel (Type I) and left in the incubator (5% CO(2)) for 3 days. These sections were dyed with calcein and propidium iodide for viability and non-viability and then observed with confocal laser scanning microscope. Images were captured digitally and examined. Since negative effects of high melting temperature of standard agar on the livability, using low melting agar to tissue blocking and high frequency - low speed vibrotome setting to cut were more preferably. In the 3 days cultures, viability/nonviability rates were indicated better values. It is concluded that, in the CNS slicing cultures, collagen coating method was an easier, effective, useful and alternative method to present techniques.