Browsing by Author "Ergul, Ali"

Now showing 1 - 4 of 4

Bazı Elma (Malus Domestica Borkh.) Genotiplerinin Ssr’lara (Simple Sequence Repeats) Dayalı Genetik Karakterizasyonu
(2018) Altıntaş, Serdar; Akpınar, Ali Emre; Karataş, Merve Dilek; Altunok, Sümeyye; Akçay, Mehmet Emin; Ergul, Ali
Türkiye, alan ve üretim potansiyelinin yanında, zengin bir elma genetik çeşitliliğine sahiptir. Ancak, farklıisimlendirmelerden ve çeşit içi varyasyonlardan kaynaklanan çeşit karmaşası yaşanmaktadır. Bu nedenle,yetiştiricilikte kullanılmayan bazı eski elma çeşitlerinin kaybolma riski görülmektedir. Ülkemiz bitki genkaynaklarının tanımlanması amacıyla; meyve türlerindeki tanımlamaların morfolojik özelliklere dayandığı, DNAdüzeyinde yürütülen çalışmalar ise sınırlı sayıda olduğu görülmektedir. Bu çalışmada, söz edilen olumsuzluklarınazaltılması amacıyla, “Atatürk Bahçe Kültürleri Araştırma Enstitüsü-Yalova” elma koleksiyonundan alınan 35 yerlive 2 referans elma genotipi, 17 SSR (Simple Sequence Repeats) markör kullanılarak tanımlanmıştır. Elde edilenverilerde benzer ve sinonim genotiplere rastlanmazken, Tavşanbaşı, Tokat, Yaz Elması ve Demir gruplarındahomonim durum tespit edilmiştir. CH01d08 lokusu, tanımlama olasılığı en yüksek lokus olarak göze çarparken,genotipler arası en yüksek benzerlik oranı %94 olarak tespit edilmiştir. Ülkemiz elma kaynaklarının SSR düzeyindetanımlanmasına yönelik, gerçekleştirilen bu çalışma bulguları, ileride yürütülecek diğer çalışmalara ön verioluşturacaktır. Aynı zamanda, bitki genetiği (genetik haritalama, gen klonlama vb.), ıslahı (kombinasyon ıslahındakullanılacak olan ebeveynlerin genetik benzerliklerinin belirlenmesi, mevcut çeşitlerde ana-baba-hibrid olasıilişkilerinin ortaya çıkarılması vb.) ve yetiştiricilik (bitkilerin tanısı ve DNA düzeyinde kontrol vb.) alanlarındakatkılar sağlayacaktır.
The Comparison of Regeneration From Root Node Explants in Solanaceae
(Hard, 2021) Oguz, Muhammad Cagri; Karata, Merve Dilek; Oguz, Ezgi; Mujtaba, Muhammad; Altinta, Serdar; Ergul, Ali
Tissue culture techniques in tomato, pepper and eggplant are important for the development of disease-resistant and high yielding varieties, which require a suitable regeneration protocol. Although shoot regeneration has been achieved by using different explants and cytokinin doses in Solanaceae species, very few studies have reported in vitro regeneration using root tissues. The current study is the first report to compare direct shoot regeneration capabilities using root node explants in three Solanaceae species (tomato, pepper, and eggplant) under three cytokinins (BAP, TDZ, and GA3) hormone. Plantlets were regenerated from the root node explants of tomato, pepper and eggplant in the media containing 0, 0.5, 1, 1.5 and 2mg/L of BAP, TDZ and GA3. Results revealed that the shoot regeneration of root node explants varied according to the species, cytokinins (BAP, TDZ and GA3) and doses of hormones. Among the species, the best shoot regeneration was observed in tomato followed by eggplant and pepper plants. While the shoot length was statistically significant in tomato, it was observed to be insignificant in pepper and eggplant. The highest number of root regeneration and root length was observed in tomatoes. The results obtained from the study will contribute to the development of successful/reproducible tissue culture protocols from roots node explants.
Identification of S-Allele Based Self-Incompatibility of Turkish Pear Gene Resources
(Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources Using Allele-Specific Pcr
(Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.