Browsing by Author "Erkec, Ozlem Ergul"

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Effects of Chronic Ghrelin Administration on Il-1, Il-6 and Tnf- Levels in Ptz Kindling Epilepsy Model in Rats
(Karger, 2021) Erkec, Ozlem Ergul; Huyut, Zubeyir; Yunusoglu, Oruc; Kara, Busra
The Effects of Cichorium Intybus Extract on Cerebellar Apoptosis and Oxidative Stress in Pentylenetetrazole-Induced Kindling Model in Rats
(Wiley-blackwell, 2016) Meral, Ismail; Gurevin, Ebru Gurel; Ustunova, Savas; Demirtas, Murat; Ozturk, Nilgun; Kiyan, Hulya Tuba; Erkec, Ozlem Ergul
Effects of Cichorium Intybus Extract on Seizure Development, Bcl-2, Siklin B1 Ve Β-Tubulin Levels in Pentylenetetrazole-Kindling Model of Epilepsy in Rats
(Wiley-blackwell, 2015) Erkec, Ozlem Ergul; Meral, Ismail; Kara, Mehmet; Kocyigit, Abdurrahim; Armagan, Metin; Ozer, Omer Faruk
Effects of Cichorium Intybus on Serum Oxidative Stress, Liver and Kidney Volume, and Cyclin B1 and Bcl-2 Levels in the Brains of Rats With Ethanol Induced Damage
(C M B Assoc, 2018) Erkec, Ozlem Ergul; Arihan, Okan; Colcimen, Nese; Kara, Mehmet; Karatas, Ersin; Demir, Halit; Ragbetli, Murat Cetin
We investigated the effects of an aqueous root extract of Cichonum iniyhus on Bcl-2 and cyclin B1 levels in the brain, kidney and liver volumes and changes of serum total antioxidant status (TAS) and total oxidant status (TOS) levels in ethanol induced damage in rats. The rats were divided into five groups non-treated controls (C), maltodextrin in tap water treated (MD), 6.4% ethanol in tap water treated (ET), Cichorium iniyhus + maltodextrin in tap water treated (CI+MD), and Cichorium intybus + 6.4% ethanol in tap water treated (CI+ET). Rats in the CI+MD and CI+ET groups were treated with 200 mg/kg water extract of Cichorium intybus. Chronic ethanol aMDinistration significantly increased cyclin B1 and decreased Bcl-2 levels in the brain and significantly decreased TAS values, increased TOS values of serum and significantly decreased kidney volume in the ET group. There was no significant difference in the liver volume or liver cell count. Our data rev ealed that ethanol aMDinistration induces an overexpression of cyclin B1 and decreases levels of Bcl-2 in rat brains and induced oxidative stress in the blood. C. intybus treatment possessed a partial amelioration effect on cyclin B1 levels and TAS values.
Effects of Leontice Leontopetalum and Bongardia Chrysogonum Extracts on Pentylenetetrazole Kindling Epilepsy Modelin Rats
(Wiley-blackwell, 2016) Erkec, Ozlem Ergul; Arihan, Okan; Bulut, Gulay; Karatas, Ersin; Meral, Ismail; Ozgokce, Fevzi
Effects of Leontice Leontopetalum and Bongardia Chrysogonum on Oxidative Stress and Neuroprotection in Ptz Kindling Epilepsy in Rats
(C M B Assoc, 2018) Erkec, Ozlem Ergul; Arihan, Okan; Kara, Mehmet; Karatas, Ersin; Erten, Remzi; Demir, Halit; Ozgokce, Fevzi
We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABA(A)) receptor positive cell number. cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ.) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats. Wistar albino rats were divided into 4 groups; Control, PTZ treated (PTZ), PTZ ;L. leontopetalum extract treated (PTZ FILE) and PTZ ;B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ, injection. PTZ treatment significantly decreased the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and bc1-2 levels and increased the total oxidant status (TOS), malondialdehyde (MDA), cyclin B1, oxidative stress index (OSI) and number of neurons that expressed GABA(A) receptors when compared to the control. LLE and BCE possessed antioxidant activity in the brain and ameliorated PTZ induced oxidative stress, decreased cyclin-B1, increased bcl-2 levels, and kept the GABA(A) receptor number similar to that of the control despite the PTZ application.
Effects of Thymus Vulgaris L. in Acute and Chronic Epilepsy Models in Rats Induced by Pentylenetetrazole
(Kafkas Univ, veteriner Fakultesi dergisi, 2019) Ozdemir, Hulya; Sagmanligil, Vedat; Erkec, Ozlem Ergul; Oto, Gokhan; Basbugan, Yildiray; Uyar, Hasan
The aim of this study was to investigate the effects of Thymus vulgaris L. (TVL) on oxidative stress, motor coordination and learning/memory in acute and chronic epilepsy models in rats induced by Pentylenetetrazole (PTZ). To this end, 64 male Wistar-albino rats were randomly divided into eight groups with 8 rats each: (1) acute control (AC), (2) acute PTZ (APTZ), (3) acute PTZ + sodium valproate (APTZ+VPA), (4) acute PTZ + TVL (APTZ+TVL), (5) chronic control (CC), (6) PTZ kindling (PTZk), (7) PTZ kindling + VPA (PTZk+VPA) and (8) PTZ kindling + TVL (PTZk+TVL). Seizures were observed for 30 min after each PTZ injection and were scored. Acute PTZ-induced seizures were created by injecting a single convulsive dose of PTZ (60 mg/kg, ip) in acute groups. PTZ kindling was produced by injecting a subconvulsant dose of PTZ (35 mg/kg, ip) every other day, with 14 injections in total. No significant difference was found among the PTZk + VPA, PTZk, and PTZk + TVL groups with regard to seizure scores. No significant difference was found among all the 8 groups in the learning/memory tests conducted using the Morris Water Maze (MWM) test and the motor activity tests conducted using the rotarod test (P>0.05). The analysis of total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) indicated that the administration of PTZ decreased the antioxidant capacity and increased the oxidant capacity. Moreover, the TVL administration established the oxidant/antioxidant balance, particularly in the chronic groups. Further studies are needed to investigate whether high doses of TVL have an effect on PTZ-induced seizure scores.
Evaluation of Dose-Dependent Effects of Nesfatin-1 in Pentyleneterazole Induced Seizures in Mice
(Kare Publ, 2021) Erkec, Ozlem Ergul
Objectives: It is well known that neuropeptides have powerful modulator effects on inhibitory and excitatory systems in the brain. Serum nesfatin-1 levels are reported to be increased in epileptic patients and experimental models of epilepsy. However, the effects of nesfatin-1 administration on acute pentylenetetrazole (PTZ)-induced seizures are unknown. The aim of this study is to investigate the dose-dependent effects of nesfatin-1 in PTZ-induced acute seizure model in mice. Methods: Animals were divided into six groups: (1) Control, (2) PTZ, (3, 4, 5, 6) PTZ+Nesfatin-1 (1, 10, 20 veya 40 mu g/kg, i.p). Physiological saline (PS, 1 mL/kg, i.p) was administrated to the mice in the control group. Nesfatin-1 or PS was administered to the experimental groups. Thirty minutes after injection of nesfatin-1 or SF, PTZ (80 mg/kg, i.p) was injected into all experimental groups to induce epileptic seizures. Latencies of the first myoclonic jerk (FMJ), generalized clonic seizures (GCS), and tonic generalized extension (TGE), and duration of TGE were determined after PTZ injection. Results: No significant difference was found between the groups in terms of latencies of FMJ, GCS, TGE, and duration of TGE. Conclusion: Nesfatin-1 did not show anti or pro-convulsant effects at all doses. In conclusion, our data demonstrated that nesfatin-1 administration had no anti/pro-convulsant effect on PTZ (80 mg/kg) induced acute seizures in mice.
Evaluation of Ghrelin, Nesfatin-1 and Irisin Levels of Serum and Brain After Acute or Chronic Pentylenetetrazole Administrations in Rats Using Sodium Valproate
(Taylor & Francis Ltd, 2018) Erkec, Ozlem Ergul; Algul, Sermin; Kara, Mehmet
Objectives: In this study, we aim to reveal the alterations (due to seizure) in the serum and brain levels of nesfatin-1, ghrelin and irisin after acute or chronic pentylenetetrazole administrations in rats using sodium valproate. Methods: 35 Wistar albino rats were randomly divided into five groups: Control, Acute Pentylenetetrazole group (APTZ), Acute Pentylenetetrazole+ Valproate group (AVPA), PTZ kindling group (PTZk) and PTZ kindling+ Valproate group (KVPA). Serum and brain levels of ghrelin, nesfatin-1 and FNDC5/irisin were determined with ELISA. Results: Serum levels of ghrelin were significantly decreased in APTZ and PTZk groups compared to the control (p < 0.01). There was a statistically significant decrease in brain levels of ghrelin in all groups compared to the control group (p < 0.01). There was a statistically significant increase in serum nesfatin-1 levels in the APTZ and PTZk groups compared to the control (p < 0.05). Serum levels of nesfatin-1 were similar to the control group in both the acute and the chronic treatment groups. There was a statistically significant increase in brain nesfatin-1 levels of the KVPA group compared to the control (p < 0.05). Serum and brain levels of FNDC5/irisin were found significantly increased in APTZ, AVPA and PTZk groups compared to the control (p < 0.01). Conclusions: Statistically significant alterations were detected in the serum and brain levels of these three peptides in both the PTZ-induced chronic epilepsy model and acute seizure model. The results of this study may suggest that the increase in FNDC5/irisin and nesfatin-1 levels, and the decrease in ghrelin levels may contribute to seizure pathophysiology. However, further studies are needed in order to confirm our hypothesis.
Evaluation of Serum Ghrelin, Nesfatin-1, Irisin, and Vasoactive Intestinal Peptide Levels in Temporal Lobe Epilepsy Patients With and Without Drug Resistance: a Cross-Sectional Study
(Assoc Medica Brasileira, 2021) Erkec, Ozlem Ergul; Milanlioglu, Aysel; Komuroglu, Ahmet Ufuk; Kara, Mehmet; Huyut, Zubeyir; Keskin, Siddik
OBJECTIVE: Epilepsy is a common disorder that affects the nervous systems of 1% of worldwide population. In epilepsy, one-third of patients are unresponsive to current drug therapies and develop drug-resistant epilepsy. Alterations in ghrelin, nesfatin-1, and irisin levels with epilepsy were reported in previous studies. Vasoactive intestinal peptide is among the most common neuropeptides in the hippocampus, which is the focus of the seizures in temporal lobe epilepsy. However, there is also lack of evidence of whether these four neuropeptide levels are altered with drug resistant temporal lobe epilepsy or not. The aim herein was the evaluation of the serum levels of nesfatin-1, ghrelin, irisin, and Vasoactive intestinal peptide in drug-resistant temporal lobe epilepsy patients and temporal lobe epilepsy (TLE) without drug resistance, and to compare them to healthy controls. METHODS: This cross-sectional study group included 58 temporal lobe epilepsy patients (24 with drug resistant temporal lobe epilepsy and 34 with temporal lobe epilepsy who were not drug-resistant) and 28 healthy subjects. Nesfatin-1, ghrelin, irisin, and Vasoactive intestinal peptide serum levels were determined using enzyme-linked immunosorbent assay. RESULTS: The serum ghrelin levels of patients with drug resistant temporal lobe epilepsy were seen to have significantly decreased when compared to those of the control group (p<0.05). Serum nesfatin-1, vasoactive intestinal peptide, and irisin levels were seen to have decreased in the drug resistant temporal lobe epilepsy group when compared to those of the control and temporal lobe epilepsy groups; however, the difference was non-significant (p>0.05). CONCLUSIONS: The results herein suggested that ghrelin might contribute to the pathophysiology of drug resistant temporal lobe epilepsy. However, further studies are needed to confirm this hypothesis.
Evaluation of the Effects of Ghrelin and Metformin on Sperm Parameters, Testosterone Hormones, and Immunohistochemical and Immunofluorescent Markers in an Experimental Diabetes Model
(Tubitak Scientific & Technological Research Council Turkey, 2023) Belhan, Saadet; Huyut, Zubeyir; Yildirim, Serkan; Erkec, Ozlem Ergul; Ozdek, Ugur
Testicular dysfunction, a severe secondary complication of diabetes, negatively affects reproductive health. Ghrelin is a peptide that plays a role in many metabolic events, such as stimulating growth hormones and regulating appetite, blood pressure, and reproduction. Metformin has antiinflammatory and antioxidant properties and is widely used to treat type 2 diabetes. Therefore, in this study, to investigate the effects of ghrelin and metformin on testicular function in experimental diabetic rats given streptozotocin, placed on a high-fat diet, and that had their sperm parameters evaluated, testosterone hormone, caspase 3 and iNOS expression, and histopathological examinations were performed. In total, 38 Wistar albino male rats were randomized into 4 groups (a control group, diabetes group, diabetes + ghrelin group, and diabetes + metformin group) with different numbers. It was determined that spermatozoa concentration, motility, and testosterone levels were lower (p < 0.001), caspase 3 and iNOS expression levels (p < 0.05) were higher, and abnormal sperm rates were higher (p < 0.001) in the diabetic group compared to the other groups. Metformin and ghrelin administered to the diabetes group increased spermatozoa concentration, motility, and testosterone levels (p < 0.001) and decreased caspase 3 and iNOS expression levels, as well as abnormal sperm rates (p < 0.05). Based on these findings, we propose that ghrelin and metformin can be used to protect reproductive health in testicular dysfunction caused by diabetes.
Ghrelin Ameliorates Neuronal Damage, Oxidative Stress, Inflammatory Parameters, and Gfap Expression in Traumatic Brain Injury
(Taylor & Francis Ltd, 2024) Erkec, Ozlem Ergul; Acikgoz, Eda; Huyut, Zubeyir; Akyol, Mehmet Edip; Ozyurt, Enver Onat; Keskin, Siddik
ObjectiveThis study investigated the effects of ghrelin on oxidative stress, working memory, inflammatory parameters, and neuron degeneration.MethodsTBI was produced with the weight-drop technique. Rats in the G+TBI and TBI+G groups received ghrelin for 7 days. The control group received saline. On the 8th day of the study, the brain and blood tissue were taken under anesthesia.ResultsA significant increase in brain GSH-PX, MDA, IL-1 beta, TGF-beta 1, and IL-8 levels and a significant decrease in CAT levels were found in the TBI group compared to the control. Serum MDA, GSH, IL-1 beta, and IL-8 levels were increased with TBI. Ghrelin treatment after TBI significantly increased the serum GSH, CAT, GSH-PX, and brain GSH and CAT levels, while it significantly decreased the serum MDA, IL-1 beta, and brain MDA, TGF-beta 1, and IL-8 levels. Histological evaluations revealed that ghrelin treatment led to a reduction in inflammation, while also significantly ameliorating TBI-induced neuron damage and vascular injuries. Immunohistochemistry staining showed that GFAP staining intensity was significantly increased in the cortex and hippocampus in TBI, and GFAP immunoreactivity was decreased with ghrelin treatment.ConclusionThe results from this study suggested that ghrelin may have curative effects on TBI.
Investigation of Effect of Exercise on Physical Parameters on Football Referees at Van Region
(Wiley-blackwell, 2015) Kina, Erol; Arihan, Okan; Erkec, Ozlem Ergul; Kara, Mehmet; Arihan, Seda Karaoz; Bektas, Yener
Investigation of Role of Vascular Endothelial Growth Factor, Annexin A5 and Apelin by Immunohistochemistry Method in Placenta of Preeclampsia Patients
(C M B Assoc, 2017) Colcimen, Nese; Bulut, Gulay; Erkec, Ozlem Ergul; Ragbetli, Murat Cetin
Preeclampsia is a disease characterized by hypertension and proteinuria occurred after 20 weeks of gestation. Preeclampsia is a major cause of maternal and fetal morbidity and mortality. The pathophysiological mechanism of preeclampsia is not known exactly yet. Preeclampsia endothelial cell dysfunction, associated with inadequate trophoblastic invasion is characterized by abnormal placentation. Vascular endothelial growth factor (VEGF) according to is an angiogenic cytokine, Annexin A5 is among endogenous peptides are both expressed from placental trophoblasts and Apelin is a multifunctional peptide and expressed by placental trophoblasts and endothelial cells. It was aimed to investigate roles of these parameters occurring in preeclampsia and to compare immunoreactivity of them in normal and preeclamptic placenta. In this study, placentas were collected from 20 normotensive pregnant women as controls, 16 mild-preeclamptic pregnant women, and 16 severe preeclamptic women. VEGF, Annexin A5 and Apelin were examined in samples of placenta tissues by streptavidin-biotin-peroxidase complex immunohistochemical methods. Immunoreactivity scores (IRS) were obtained for each parameter. VEGF and Apelin IRS were increased significantly in preeclamptic groups compared with control group (p <0.026, p<0.002 respectively). But Annexin A5 IRS was decreased significantly in preeclamptic groups compared with control group (p<0.04). In correlation with the intensity of disease, increase in VEGF and Apelin, and decrease in Annexin A5 supports roles of hemo-dynamic alterations in fetoplacental circulation and structural alterations in uteroplacental bed occurring in preeclampsia.
Pentylenetetrazole Kindling Epilepsy Model
(Kare Publ, 2015) Erkec, Ozlem Ergul; Arihan, Okan
Epilepsy is one of the most common neurologic disorders affecting approximately 1% of the general population, and no alleviation was achieved in one third of the patients medicated with antiepileptic drugs. Current treatments of epilepsy are symptomatic and have more anti- seizure effects than antiepileptic effects. These therapies do not cure epilepsy. Basic mechanisms of epilepsy have not entirely been clarified yet. Using seizure and epilepsy animal models, our comprehension about basic mechanisms underlying epileptogenesis has improved. In addition, animal models of epilepsy and seizures are very useful in the discovery and development of new antiepileptic drugs. Pentylenetetrazole (PTZ) is widely used in antiepileptic drug discovery studies, and PTZ kindling model is very important to understand the pathophysiology of epilepsy. In this review, current information about PTZ kindling model was given in this aspect.
Protective Effects of Omega-3 Against Acute Tributyltin Toxicity in Rat
(Parlar Scientific Publications (p S P), 2017) Dogan, Abdulahad; Arihan, Okan; Erkec, Ozlem Ergul; Celik, Ismail
The aim of this study was to determine the in vivo healing and possible protective effects of Omega-3 against acute Tributyltin (TBT) toxicity in different tissues of rats. TBT, a tin-containing organometallic antifouling compound, is known for its adverse effects on aquatic as well as terrestrial organisms. Five groups, with seven rats each, were used in the experiments. The groups were designed as: (I) normal control, (II) vehicle control, (III) TBT (5 mg/kg/day) (IV) Omega-3 (250 mg/kg/day), and (V) TBT+Omega-3 (5 mg/kg/day + 250 mg/kg/day). Liver, kidney, spleen, and gonad tissues were assessed for their superoxide dismutase, glutathione peroxidase (GPx), glutathione reductase, and glutathione S-transferase activity levels, and changes in the glutathione (GSH) and malondialdehyde (MDA) levels, as well as serum biochemical parameters. TBT administration was found to alter the lactate dehydrogenase, total cholesterol, and low-density lipoprotein cholesterol significantly. MDA in the kidney showed a significant increase in the TBT administration compared to the control and vehicle, and a significant decrease in the Omega-3-administered groups. In the liver and spleen, the GPx showed a significantly low level of TBT compared to the control and vehicle, whereas it was significantly increased in the Omega-3 and TBT+Omega-3 groups compared to the TBT group. As a result, TBT was found to cause an increase in the lipid peroxidation, a deterioration in the lipid profile and biochemical parameters, a decrease in the GSH, and a loss of antioxidant defense enzyme activity, whereas Omega-3 was found to ameliorate some of those hazardous impacts.
Relationships Between the 2d:4d Digit Ratio, Waist Circumference, Hand Preferences, Weight, Height, Waist-To Ratio and Bmi in a Turkish Population
(Soc Chilena Anatomia, 2019) Erkec, Ozlem Ergul
The 2D:4D is thought as a biomarker for prenatal androgen exposure. Some studies have reported a significant difference between the sexes. Earlier studies reported variations in different ethnic and geographic groups. 2D:4D is related to several medical conditions including cardiovascular diseases (CVD) and metabolic syndrome (MetS). The present study investigated the relationships between hand preference, ratio of the index finger (2nd digit:2D) and ring finger (4th digit:4D) lengths (2D:4D), height, waist-to-height ratio (WHtR), waist circumference (WC) and weight, body mass index (BMI) in a Turkish population. The study comprised 118 healthy subjects (68 males and 50 females). 2D and 4D finger lengths and some anthropometric traits (height, weight, WC) were measured. The BMI and WHtR were calculated. Hand preference was determined by using a questionnaire (Edinburgh handedness inventory). Geschwind scores were calculated to evaluate the degree of hand preference. The mean age was 26.74 (female 27.86, male 25.89). The right hand 2D:4D was found significantly lower in males (0.9797) than in females (0.9922) (p<0.001), but the left hand 2D:4D was insignificant. Significant correlations (negative) were observed between the 2D:4D (both left and right) and WHtR; the 2D:4D ratio (both left and right) and WC in males. However, in females, these correlations were insignificant. The R2D:4D was sexually dimorphic in a Turkish population. There were significant differences between strong right (SR) and weak left (WL) in terms of the R2D:4D. However only 5 subject's hand preference was found WL. Therefore, further studies are needed to determine the relationships between hand preference and R2D:4D in Turkish population. Further studies are needed to determine whether a larger sample population alters these possible associations between the ratio of 2D:4D and other investigated traits in a Turkish population.