Browsing by Author "Eroglu, Ayten"

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Dna Methylation Change and Antioxidant Enzyme Activity of Drought Stress and Putrescine Treatment in Ancient Wheat (Triticum Monococcum L.)
(Springer Heidelberg, 2024) Demirel, Serap; Eroglu, Ayten; Eren, Baris; Demirel, Fatih
Drought stress reduces crop productivity and harms cereals on morphological, physiological, biochemical, and molecular levels. Yet, exogenous application of certain active compounds like putrescine may mitigate these drought-related consequences. In this study, five distinct drought treatments (0, - 2, - 4, - 6, and - 8 bar) and four distinct priming treatments with putrescine (0, 0.01, 0.1, and 1 mM) were used. According to the findings of the enzyme activity, the activities of enzymes like superoxide dismutase (SOD) and catalase (CAT) all reduced when the plants were subjected to drought stress; however, it was determined that the putrescine treatment at - 2 bar PEG6000 resulted in an increase in the activity of CAT. iPBS (inter-primer binding site) markers were employed to detect cytosine methylation in treated wheat plants (Triticum monococcum L). The results confirmed that DNA methylation alterations were triggered by drought stress. The study revealed a positive correlation between drought intensity and cytosine methylation levels, while it was found that the application of putrescine resulted in a decrease in methylation levels and conferred a protective effect against drought-induced stress. Moreover, results of in silico docking that showed putrescine can bind to DNA methyltransferase2, that is, the decrease in DNA methylation may be related to this. Drought-stressed wheat is less methylated after being treated with putrescine, according to in silico docking and CRED-iPBS data. Testing the protective effects of polyamines on plants is an area that needs further attention.
Natural Diversity of Crataegus Monogyna Jacq. in Northeastern Türkiye Encompassing Morphological, Biochemical, and Molecular Features
(Mdpi, 2025) Erkek, Bora; Yaman, Mehmet; Sumbul, Ahmet; Demirel, Serap; Demirel, Fatih; Coskun, Omer Faruk; Eroglu, Ayten
Hawthorn (Cratageus monogyna Jacq.), one of the important wild fruit species in Turkey, is an important fruit species in many countries due to its use in traditional medicine, landscaping studies, and the food and beverage industry. In this study, morphological characterization revealed significant variation among genotypes, with fruit weight ranging from 0.55 g to 2.87 g, fruit width from 9.74 mm to 18.39 mm, and leaf width from 19.53 mm to 41.19 mm. Genotype-dependent variations were observed in the total phenolic content (151.34 mg-491.88 mg GAE 100 g-1 flesh), the total flavonoid content (16.74-48.13 mg CAE 100 g-1 flesh), the total anthocyanin content (26.34-79.79 mg cyn-3 gluc 100 g-1 flesh), and the antioxidant activity (29.20% to 56.70%). HPLC-based phenolic profiling identified substantial variations in key bioactive compounds, with the highest levels of chlorogenic acid (16.16 mg 100 g-1 fw), caffeic acid (10.21 mg 100 g-1 fw), epicatechin (13.83 mg 100 g-1 fw), rutin (74.05 mg 100 g-1 fw), and protocatechuic acid (2.00 mg 100 g-1 fw). ISSR marker-based molecular analysis revealed a high degree of genetic polymorphism (89.12%), with 55 out of 62 bands classified as polymorphic. The polymorphic information content values ranged from 0.34 to 0.44. The Jaccard similarity coefficient ranged from 0.04 (M9 and M16) to 0.63 (M17 and M3), indicating substantial genetic variability. The tanglegram analysis comparing genetic and morphological-biochemical dendrograms yielded an entanglement score of 0.714, indicating an alignment between molecular and phenotypic data. These findings show that hawthorn genotypes from the Kelkit Valley exhibit extensive genetic and biochemical diversity, which is critical for conservation efforts, breeding programs, and the development of high-value medicinal and functional food products.
Phenolic Compound Production Increased in Vitro Regenerated Cichorium Intybus L
(Mdpi, 2023) Abas, Yousif Abdullah; Eroglu, Ayten; Dalar, Abdullah; Turker, Musa; Ozdemir, Fethi Ahmet; Solowski, Gawel
Chicory (Cichorium intybus L.) is a low-height perennial or biennial herb from the family of Asteraceae. Investigation of different in vitro regeneration strategies of Cichorium intybus and increasing the number of secondary metabolites in vitro regenerated plant samples were the aims of the research. Callus and plant regenerations were achieved in basal plant growth media supplemented with plant growth regulators (PGRs). Whole plant regeneration was carried out by direct organogenesis from leaf explant in Murashige and Skoog (MS) and B5 media supplemented with naphthalene acetic (NAA) acid and indole-3-butyric acid (IBA). The highest callus quantity was produced in MS medium supplemented with indole-3-acetic acid (IAA) and benzyl amino purine (BAP). The combination and concentrations of PGRs used in MS and B5 media not only provided root and shoot formation with callus, but also caused a change in the amounts of phenolic components. In addition, some PGRs used caused an increase in the number of phenolic compounds in callus and shoots developed from the leaf explant. When plants that grow in vitro and in vivo are compared with each other, it has been determined that plants grown in vivo contain higher amounts of some phenolic compounds. In vivo and in vitro samples were extracted in ethanol/water (80:20 v/v). The analysis of phenolic compounds (caftaric, chicoric, and chlorogenic acids and esculin) were performed in high-performance liquid chromatography (HPLC) and inulin was in UV spectrophotometry. The caftaric and chlorogenic acids and inulin concentrations were higher in vivo samples than that in vitro. Contrarily, esculin, and chicoric acid concentrations were higher in the in vitro regenerated samples. The higher concentration of valuable compounds in the in vitro regenerated samples, especially in callus tissue, gives hope for large-scale production of secondary metabolites under laboratory conditions.
Vector Construction for Transformation of Tobacco With a Nac-Type Transcription Factor, Tanac69-1
(Elsevier Science Bv, 2016) Eroglu, Ayten; Baloglu, Mehmet Cengiz; Yucel, Meral