Browsing by Author "Ertas, Fatma"

Now showing 1 - 4 of 4

Detection of Cryptosporidium Spp. in Calves Through Nested Pcr and Kinyoun's Acid-Fast Methods in Igdir, Turkey
(Health & Environment Assoc, 2021) Ertas, Fatma; Ayan, Adnan
The present study was conducted to determine the prevalence of cryptosporidiosis by using Kinyoun's acid-fast and Nested PCR methods in 0-01 month aged calves in Igdir Province, Turkey. A total of 100 calves were used in this study. The calves belonging to the Igdir Provincial Directorate of Agriculture and Forestry, were diagnosed to have diarrhea on the basis of their clinical and anamnesis. In the microscopic examinations of 100 samples, 34 (34%) Cryptosporidium spp. were detected in Kinyoun's acid-fast whereas in the Nested PCR method it was found 38 (38%). Accordingly, attention was drawn to the necessity of complying with protective measures due to its zoo notic importance.
Effect of Imidocarb on Dna Damage in Sheep With Babesiosis
(Kafkas Univ, veteriner Fakultesi dergisi, 2022) Oner, Ahmet Cihat; Ayan, Adnan; Orunc Kilinc, Ozlem; Usta, Ayse; Ertas, Fatma
In this study, it was aimed to determine the DNA damage using the comet assay, which specifically shows DNA damage in naturally Babesia spp.-infected sheep and to evaluate the damage before and after imidocarb application. Blood samples obtained from 10 infected sheep with positive clinical signs and symptoms of babesiosis and whose diagnosis was confirmed by Giemsa staining and PCR methods, and blood samples from 10 healthy sheep were used as study material. DNA damage was examined by the comet assay from the blood samples of the infected patient group and the control group obtained during the disease and after the treatment, and the results were compared with statistical methods. When DNA damage was examined in sick animals diagnosed with babesiosis, the tail length and the tail moment values were found to be statistically significantly higher than the control group (P<0.0001). According to the results obtained after imidocarb application, it was determined that DNA damage and tail moment decreased statistically with imidocarb, and the difference was statistically significant, and the values were higher than the control group (P<0.0001). As a result, Babesia infection can cause DNA damage, has been confirmed by the determination of direct DNA damage using the comet assay, and imidocarb given for treatment was successful and reduced the damage.
The Effects of Some Cephalosporins on Acetylcholinesterase and Glutathione S-Transferase: an in Vivo and in Vitro Study
(Taylor & Francis Ltd, 2019) Turkan, Fikret; Huyut, Zubeyir; Demir, Yeliz; Ertas, Fatma; Beydemir, Sukru
Background: Glutathione S-transferase (GST) and acetylcholinesterase (AChE) are important enzymes in the metabolism. GSTs are primarily available in phase II metabolism. AChE is vital for neurodegenerative disorders. Subjects and methods: The in vitro and in vivo effects of cefoperazone sodium (CFP), cefuroxime (CXM), and cefazolin (CZO) were investigated on GST and AChE activity in the present study. GST was purified using Glutathione-Agarose affinity chromatography. Results: K-i constants of CFP, CXM, and CZO were 0.1392 +/- 0.02, 1.5179 +/- 0.33, and 1.006 +/- 0.11 mM for GST and 0.3010 +/- 0.07, 0.3561 +/- 0.09, and 0.3844 +/- 0.04 mM, for AChE, respectively. The most effective inhibitor was CFP for both enzymes in in vitro. CZO (50 mg/kg), CXM (25 mg/kg), and CFP (100 mg/kg) inhibit in vivo GST and AChE activities. CXM had the most effective in vivo inhibition on AChE and GST. Conclusions: CZO, CXM, and CFP are effective AChE and GST inhibitors in both in vitro and in vivo.
Evaluation of Oxidant, Antioxidant, Interleukin and Biochemical Parameters in Sheep With Cystic Echinococcosis and Dicrocoeliosis
(Polish Soc veterinary Sciences Editorial office, 2025) Ertas, Fatma; Komuroglu, Ahmet Ufuk; Kilinc, Ozlem Orunc; Basbugan, Yildiray; Yuksek, Nazmi
Aim: Endoparasitic infections have long posed a significant threat to the sheep industry, leading to substantial economic losses. Among these parasites, Dicrocoelium dendriticum and, particularly, Echinococcus granulosus are globally important zoonotic parasites. This study aimed to investigate the levels of oxidative stress markers, antioxidant parameters, interleukins, and biochemical indicators, as well as their correlations, in sheep affected by cystic echinococcosis and dicrocoeliosis. Methods: In this study, various biochemical and immune-related parameters were assessed. Serum samples collected from infected sheep were analyzed using ELISA to measure interleukin-18 (IL-18), interleukin-10 (IL-10), interleukin-6 (IL-6), interleukin-4 (IL-4), NGAL, neopterin, myeloperoxidase, SOD, GPX, catalase, GSH, and MDA. Additionally, spectrometric analysis was performed to determine the levels of AST, ALT, GGT, albumin, globulin, total protein, ALP, and iron. Results: Serum MDA levels were found to be significantly elevated in infected sheep compared to the healthy group. In contrast, serum SOD and GSH levels in infected sheep were significantly lower than those in healthy sheep. Additionally, serum AST and ALT activities were markedly higher in the infected group. Interleukin levels showed no significant differences between the two groups. Overall, markers of liver function and oxidative stress were notably increased in infected sheep. Conclusion: It was concluded that oxidative stress and liver damage occurred in the infected group, and their immune system was actively involved in the response. This study not only adds to academic knowledge, but should also help veterinarians and breeders working in the field to make more accurate decisions. Thus it is expected to contribute directly to herd health, animal welfare, economic efficiency, and public health. Correlation data may also be useful in guiding the treatment process.