Browsing by Author "Ertas, M."

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Antimicrobial Properties of Multimetallic of Silver Compounds Immobilized on Active Carbon and Nano Carbon Tubes
(Elsevier B.V., 2021) Kazici, H.Ç.; Yönten, V.; Kivanç, M.R.; Ertas, M.; Salman, F.; Yayla, M.
In this paper, some bi- and tri-metallic silver compounds (Ag50Ni50/CNT, Ag90Ni10/CNT, Ag70Ni30/CNT, Pd10Co10Ag80/AC, Pd20Co30Ag50/AC, Pd30Co30Ag40/AC, (AC; active carbon) (CNT; carbon nanotube)) were synthesized with the polyol method. Antibacterial activities against Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25952, Pseudomonas aeruginosa ATCC 27853 and Bacillus cereus ATCC 29213, and antifungal activity against Candida albicans ATCC 90028 were investigated. The standard protocols were evaluated in the experimental process. The antibacterial activity assay results were correlated with the morphological characterization of the material (scanning electron microscope energy dispersive X-ray spectroscopy). It was determined that all nanomaterials (NMs) had effective antifungal activity against C. Albican fungi, but Ag70Ni30/CNT had better antifungal effects than the other NMs. Only Ag70Ni30/CNT had antibacterial activity against B. cereus and P. aeruginosa bacteria pathogens. Therefore, the relevant NMs were introduced to the literature with this feature of antifungal and antibacterial activities. © 2021
Identification of Biodiversity of Some Streptomyces Species and Determination of a Restriction Fragment Length Polymorphism (Rflp) Profile of 16s Rdna Gene Region
(Medwell Journals, 2014) Ozdemir, K.; Ogun, E.; Ertas, M.; Acar, S.; Atalan, E.
In this study, soil samples from the roots and peripheral regions of some agricultural plant species growing in the Van region (such as sainfoin, bean, malus, wheat and sugar beet) were collected between May and June 2004 and 139 strains of Streptomyces bacteria were isolated from these samples. Moreover, researchers determined the physicochemical characteristics of these soil samples. Streptomyces bacteria were color-grouped and the Diaminopimelic Acid (DAP) forms were detected followed by the determination of then phenotypic characteristics. When these isolates were divided into 39 color groups, 20 strains were found to contain L-DAP and therefore were confirmed as members of the genus Streptomyces. The species were identified using the TAXON Software and similarity dendrograms were constructed by the Unweighted Pair-Group Method with an Arithmetic average (UPGMA) cluster analysis using the Multi-Variate Statistical Package (MVSP 3.1). Genomic DNA was isolated from these bacteria and 16S rDNA gene was amplified by PCPv followed by restriction digestion with Bspl43I, Haelll, Mnll and SphI for Restriction Fragment Length Polymorphism (RFLP) analysis. In the dendrogram obtained at the end of the RFLP analysis, four groups of two or more members and six 1 -member groups were established according to the similarity ratio criterion. © Medwell Journals, 2014.
Sium Sisarum L. Var. Lancifolium ( Bieb.) Thell a Traditional Spice From Eastern Anatolia: Chemical Composition and Biological Activities
(Pamukkale University, 2022) Mukemre, M.; Dalar, A.; Taylan, S.B.; Ertas, M.
Traditionally consumed food and spices are significant sources in the daily life diet and constitute a large portion of the cuisine in Eastern Anatolia in Turkey. However, limited data available necessitate further analysis of their chemical composition and health attributing properties. This study aims to present phytochemical composition and biological activities of Sium sisarum var. lancifolium, a commonly consumed spice and food species in the region. Analytical studies to date have revealed the presence of high levels of phenolics (chlorogenic acid and isoquercetin) and volatiles (a-terpinene, camphene, cyclohexene, carene and p-cymene), which exhibits significant potential of digestive enzyme suppressive and antioxidant abilities. Data collected in this study suggest the use of Sium sisarum plant to obtain nutraceuticals and/or biotherapeutic agents that are able to regulate oxidative stress and enzyme activities. © IJSM 2022.
Use of Random Amplified Polymorphic Dna Pcr To Distinguish Micromonospora Species Isolated From Soil
(Medwell Journals, 2014) Ertas, M.; Ozdemir, K.; Atalan, E.
Recently with the advancement of molecular methods, studies that determine intraspecific genetic polymorphisms have yielded substantial results. The aim of this study was to use Random Amplified Polymorphic DNA PCR (RAPD-PCR) analysis to determine the differences among Micromonospora species isolated from the soil. M3 medium with antibiotics was used to isolate the species from the soil and 65 Micromonospora species were identified. Total DNA analysis was performed for the different color groups which were obtained as a result analysis of the species. Sm5R, Sm6F, DAF4, Ml 3, 27F and 1492R primers were used for RAPD-PCR analysis and the band profiles of the species were obtained. The dendrogram generated according to the simple matching coefficient method clearly showed the differences detected among the species. A total of seven groups were identified along with two main groups. Five of these groups had a single member, one had 3 and one had 2 members. © Medwell Journals, 2014.