Browsing by Author "Esrefoglu, Mukaddes"

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The Effect of Prenatal Exposure of a Non-Steroidal Anti-Inflammatory Drug on the Optic Nerve of Female Rats: a Stereological, Histological, and Electron Microscopic Study
(Taylor & Francis Ltd, 2013) Kaplan, Suleyman; Esrefoglu, Mukaddes; Aktas, Abit; Gul, Mehmet; Onger, Mehmet Emin; Altunkaynak, M. Eyup; Ragbetli, Murat Cetin
Objective: Non-steroidal anti-inflammatory drugs (NSAIDs) can have adverse effects for in both mother and fetus following administration during the prenatal period. If given during pregnancy, diclofenac sodium (DS), an NSAID, is given during the pregnancy, may also affect the development of the central nervous system (CNS) or related structures. Methods: Pregnant rats were separated into pure control (PG), saline (SG) and diclofenac groups (DG). A daily dose of 1 mg/kg of DS and 1 mL/kg saline was injected intraperitoneally to the DG and SG groups, respectively, from the 5th gestation day for a 15 day of period; the PG group received no treatment. After spontaneous delivery, female offspring were obtained from all groups. After the 20th week of postnatal life, the animals (n = 6 for each group) were perfused and the right optic nerves were resected. Sections were subjected to stereological and histological analysis. Results: There were no significant differences (p > 0.05) between PG, SG and DG groups with respect to myelin thickness, axonal cross-sectional area, axon numerical density, total section area of optic nerve and axon number. Conclusions: Histological and stereological results indicated that treatment with DS or saline produced undesirable effects on female rat optic nerve development and myelinization with respect to morphology.
Effects of Cichorium Intybus on Gabaa Receptors and Apoptosis in Pentylenetetrazole-Induced Kindling in Rats
(Kare Publ, 2021) Ergul Erkec, Ozlem; Meral, Ismail; Kara, Mehmet; Esrefoglu, Mukaddes; Tok, Olgu Enis; Ustunova, Savas; Armagan, Metin
Objectives: This study was designed to determine the effects of Cichorium intybus (CI) on apoptosis and GABA(A) receptor density in the brains of rats in pentyleneterazole induced kindling. Methods: The rats were divided into three groups: Control group, pentylenetetrazol administered (PTZ) group, and PTZ+CI extract administered (PTZ+CI) group. Control group received only physiological saline (0.5 ml). PTZ (35 mg/kg) injected to the animals in the PTZ and PTZ+CI groups. The CI extract (200 mg/kg) was also administered to the PTZ+CI group. A 75 mg/kg challenge dose of PTZ was administrated to the PTZ treated groups, on the 12th injection. Results: A significant increase was found in the number of neurons expressing the GABA(A) receptor in the brain tissue (hippocampus and cerebral cortex) of the PTZ group when compared to the control. The density of GABA(A) receptor of the neurons in the cerebral cortex significantly increased in PTZ administered groups compared to the control.The number of apoptotic neurons was found non-significant between groups in the brain. Conclusion: CI treatment prolonged the onset of the first seizure activity and seizure latency at a convulsive dose, and kept the number of GABA(A) receptors close to that of the control in the hippocampus.