Browsing by Author "Evyapan, G."

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[1]Vitamin B12 Enhances Cisplatin Efficacy via Apoptosis and MAPK/ERK1-2, P38, PARP-1 Modulation in Prostate Cancer
(Yuzuncu Yil Universitesi Tip Fakultesi, 2025) Evyapan, G.; Ozdem, B.; Tekedereli, I.
Introduction: Prostate cancer (PC) is the most common malignancy among men and remains a major cause of cancer-related mortality worldwide. Cisplatin is a widely used chemotherapeutic agent in cancer treatment. Vitamin B12 has been shown to play a role i n enhancing the efficacy of certain cancer drugs when used in combination therapies. This study investigates the antitumor effects and mechanisms of action of B12 and Cisplatin combination therapy in prostate cancer cells. Materials and Methods: The clonogenic assay was used to determine the fraction of surviving cells after treatment. The MTS assay and flow cytometry were performed to assess the impact of B12 and Cisplatin on cell proliferation and apoptosis, while Western bl ot analysis was used to examine the expression of key signaling proteins involved in these processes. Results: Our results revealed that the combination treatment of B12 and Cispalatin significantly inhibited the proliferation and viability o f the PC cell line. Also, clonogenic assay indicated that B12 and Cisplatin combination treatment inhibited the colony formation. Moreover, the combined treatment showed a 2.3-fold increase in P38 and a 1.8-fold increase in PARP-1 protein expression compared to control. In addition, MAPK/ERK1-2 and Bcl-2 protein expression were significantly reduced by approximately 40% and 45% respectively in the combination treatment. Conclusion: Our findings suggest that the combination of B12 and Cisplatin enhances the antitumor effects of Cisplatin by promoting apoptosis and modulating key signaling pathways, including P38, PARP-1, and MAPK/ERK1-2. These findings, supported by significant reductions in cell viability (up to 50%), suggest a promising role for B12 and Cisplatin combination therapy. Further in vivo and clinical studies are warranted to validate these preliminary in vitro findings. © 2025, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Education and Regular Examination on the Prevalence of Head Louse Infestations in Adana
(Galenos Publishing House, 2022) Kavur, H.; Özkurt, H.; Büyükkatran, F.; Evyapan, G.; Kalkan, S.; Çelik, Z.; Alptekin, D.
Objective: The current study provides training to parents and teachers about pediculosis in schools in three villages in Adana to measure their knowledge level by conducting surveys and to determine the prevalence of pediculosis in these foci. Methods: Pre-and post-questionnaires including 30 questions about pediculosis were handed to parents and teachers. The answers were analyzed with the Pearson correlation analysis. Overall, 418 school pupils s were examined for lice. The results of the head louse control were analyzed by the chi-square test and t-test. Results: We observed that the level of awareness increased in parents and teachers. Additionally, the gender of both teachers and parents was determined as the most important factor in increasing this awareness. Because of interventions for the control of head and lice, the prevalence of pediculosis decreased from 15.22% to 1.71%. Conclusion: It is very important that parents and teachers are aware of the health problems related to pediculosis, while regular combing of school children may be essential for the control of this common infestation. © 2022 Turkish Society for Parasitology.
Gum Arabic Suppresses Proliferation and Induces Mitochondrial-Mediated Apoptosis in MCF-7 Breast Cancer Cells via the Bcl-2/Bax Signaling Pathway
(Ondokuz Mayis Universitesi, 2025) Evyapan, G.; Özdem, B.
Breast cancer is the most frequently diagnosed cancer among women worldwide, and despite advances in treatment modalities, there remains a critical need for more effective therapeutic strategies. This study investigated the impact of Gum Arabic (GA) on the proliferation and apoptosis of MCF-7 breast cancer cells. The impact of Gum Arabic on cellular viability was assessed using an MTS assay, while its effect on long-term proliferative potential was evaluated via a colony formation assay. To determine the mode of cell death, caspase-3/7 activity assays and Annexin V-FITC/propidium iodide staining were employed. Since MCF-7 cells lack functional caspase-3, the observed caspase-3/7 activity is likely attributable to caspase-7. Additionally, Western blotting was conducted to analyze changes in the expression of key apoptotic proteins. The results revealed that treatment with Gum Arabic led to a dose-dependent reduction in both cell viability and colony formation ability. Moreover, apoptosis was significantly induced in the treated cells. At the molecular level, Gum Arabic administration resulted in a pronounced downregulation of the anti-apoptotic protein B-cell lymphoma 2, along with upregulation of the pro-apoptotic proteins Bcl-2-associated X and caspase-9. These findings demonstrate that Gum Arabic not only suppresses proliferation but also promotes programmed cell death in breast cancer cells through modulation of intrinsic apoptotic pathways. While the results provide preliminary evidence of anticancer potential, further studies in additional breast cancer models, as well as in vivo and clinical investigations, are required before any translational or therapeutic conclusions can be drawn. © 2025 Elsevier B.V., All rights reserved.
Inhibition of Autophagy by ATG5 siRNA Transfection Enhances Anti-Cancer Effects of Gum Arabic, Promotes Oxidative Stress-Mediated Apoptosis and Affects DNA Damage and Mitochondrial Membrane Potential in Ovarian Cancer Cells
(Springer International Publishing, 2025) Evyapan, G.; Özdem, B.
Gum Arabic (GA) is a clinically safe plant-derived polysaccharide with potential anti-cancer activity. We evaluated the effects of GA alone and in combination with ATG5 siRNA-mediated inhibition of autophagy in chemoresistant A2780-ADR ovarian cancer cells. GA at a concentration of 30.68 µM reduced cell viability to 47 ± 3% at 72 h and increased intracellular ROS 2.3-fold (n = 3, p < 0.001). The GA + ATG5 siRNA combination further decreased viability to ~ 30% and markedly enhanced apoptosis (Annexin V/PI, p < 0.001). Western blot analysis revealed increased p53 protein levels and decreased Bcl-2 protein levels, as well as altered P-Chk1 protein levels, which are consistent with apoptosis associated with DNA damage. GA also caused a loss of mitochondrial membrane potential and treatment-dependent changes in UCP4/5 expression, indicating mitochondrial stress. These findings identify GA, particularly in combination with autophagy inhibition, as a low-toxicity agent with significant anti-proliferative effects in vitro. The study is limited to cell models; in-vivo validation and pharmacokinetic/delivery studies are required before clinical translation. © 2025 Elsevier B.V., All rights reserved.