Browsing by Author "Furan, M. Alp"

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Bazı Makarnalık Buğday Çeşitleri ile Yeni Geliştirilen Hatlarda Genetik İlişkilerin Rapd Markörleriyle İncelenmesi
(2008) Tonk, Fatma Aykut; Akçalı, R. Refika; Furan, M. Alp; Yüce, Süer
Ege Bölgesi’nde verim yönünden ön plana çıkan dört makarnalık buğday çeşidi ile uzun yıllar yapılan denemeler sonucu ümitvar görülen CIMMYT kökenli üç introdüksiyon hattında, moleküler markör yöntemlerinden RAPD metodu ile söz konusu genotipler arasında genetik olarak benzerliklerin ve farklılıkların incelenmesi bu çalışmanın amacını oluşturmuştur.Yapılan DNA analizleri sonucunda, çalışmada incelenen çeşit ve hatların genetik olarak birbirine yakın olduğu ve bunlara ait ortalama genetik benzerlik oranının 0.86 olduğu saptanmıştır. Ayrıca Ege-88 ile Yavaros-79 çeşitlerinin birbirlerine en yakın genotipler olduğu anlaşılmıştır.
Buğdayda Sarı Pasa Dayanıklı ve Duyarlı Bazı Çeşit ve Hatların Ssr Analizleri
(2009) Yüce, Süer; Furan, M. Alp
Bu çalışmada amaç, sarı pasa dayanıklı ve duyarlı bazı ekmeklik buğday genotipleri arasında pasa dayanıklılık açısından DNA düzeyinde SSR markörleri yardımıyla genetik analizlerin incelenmesidir. Sarı pasa dayanıklı ve duyarlı olan bu genotiplerin arasındaki genetik ilişki SSR (Simple Sequence Repeat) markörlerinin kullanılması ile incelenerek belirlenmeye çalışılmıştır. Araştırmada sarı pasa duyarlı yerli çeşit ile dayanıklılık geni barındıran yakın izogenik test hattı melezlenmiş, ebeveynler ile bunların F1 ve F2 dölleri üzerinde SSR moleküler markörleri kullanılarak genetik analizleri yapılmıştır. Çalışmanın SSR analizleri forward ve reverse olmak üzere 5 SSR primeri kullanılarak gerçekleştirilmiştir. Yapılan moleküler markör analizi sonucunda sarı pasa duyarlı yerli çeşit ile tek gen dayanıklı hat arasındaki genetik benzerlik ve farklılıklar moleküler düzeyde ortaya konmuştur. Ayrıca bu çalışmayla moleküler markör kullanımının dayanıklılık ıslahındaki önemi ve gerekliliği birkez daha vurgulanmıştır.
Characterization of Various Hypericum Perforatum Clones by Hypericin and Rapd Analyses
(Friends Science Publ, 2011) Tonk, Fatma Aykut; Giachino, R. Refika Akcali; Sonmez, Cigdem; Yuce, Suer; Bayram, Emine; Telci, Isa; Furan, M. Alp
Hypericum perforatum L. is a pharmaceutically important medicinal plant with antidepressant, antiviral, antibacterial and anticancer effects of its metabolites. The hypericin contents and genetic structures of superior H. perforatum clones were identified by spectrophotometric analysis and random amplified polymorphic DNA (RAPD) markers, respectively. At the first harvest the hypericin content of the clones was higher than second harvest and higher in 2007 compared to 2008. For initial screening, 38 random primers were used and 26 of them detected a total of 378 scorable fragments. Effective multiplex ratio (EMR), average heterozygosity (H-av), marker index (MI), resolving power (RP) and polymorphic information content (PIC) of the primers were calculated for RAPD marker system. Genetic similarity value among the clones varied from 0.40 to 0.93 and the average genetic similarity values was determined as 0.60. Clustering based on RAPD data showed a partial relationship on a regional basis in the clones. Cluster analyses of hypericin contents and RAPD markers grouped the clones in two major clusters and significant correlations were observed between them. In conclusion, combined analysis of RAPD markers and hypericin content is an optimal approach for further progress and breeding programs. (C) 2011 Friends Science Publishers
Exploring Secondary Structures Within the Nuclear Ribosomal Dna Internal Transcribed Spacers (Nr Dna Its) for Enhanced Phylogenetic Insights in Medicago Sativa (Alfalfa)
(Springer, 2024) Furan, M. Alp; Genli, Gulistan; Yildiz, Mehtap; Sun, Hyeon-Jin
ITS sections, which are situated inside the ribosomal DNA operon, are recognized for their high levels of diversity and slow rate of evolution, which makes them important for plant phylogenetic studies. The objective of this work is to use the internal transcribed spacer (ITS) regions of 18 alfalfa (Medicago sativa) genotypes/varieties to determine their genetic relationships and to verify possible genetic markers for variety identification and classification. This has been done by validating the secondary structures of the ITS regions and observing the effects of the relationships among the lowest energy state, the total number of possible secondary structure hairpins, and (GC) content. The results indicated that the length of the ITS sequences varied between 426 and 629 nucleotides and that the G + C content of the ITS region varied between 46.6 and 50%. In this study, the analysis of molecular variance (AMOVA) was used to validate the effectiveness of configuration analysis and revealed that fluctuations in the data accounted for a substantial amount of the observed total variance. Moreover, 94 percent of the genotypes analyzed exhibited a significant degree of variety. This shows that a large fraction of the observed variation is likely attributable to genetic variables. Population four, comprising Bilensoy80, Emily, Quin, Vendor, and Felicia, had the highest degree of heterozygosity at 68.8%, whereas population two, including Gozlu1, Prosementi, Nimet and Local (Van), had the lowest level at 37.5%. The original Shannon's approach as an unbiased estimator employed in population genetics research validated the differences among alfalfa genotypes with the AMOVA analysis results of this study. Individual differences were found to be 59%, whereas population differences were found to be 41%. There have been a few ITS studies on Medicago sativa that have utilized ITS as a phylogenetic marker to estimate connections and define new taxonomic categories (e.g., tribes). However, our research also includes an analysis of the secondary structure of these sequences and the results of this study imply that ITS sequence and secondary structure data can be utilized to understand the intraspecific genetic makeup of different alfalfa varieties.
Phylogenetic Inference and Secondary Structure Predictions of Turkish Genotypes of Coriandrum Sativum (L.) Based on Its4 and Its5 Nrdna Sequences
(Springer, 2022) Furan, M. Alp; Yildiz, Mehtap; Karatas, Merve Dilek; Chung, Yong Suk
Coriander, Coriandrum sativum L., is an important medicinal plant belonging to the Apiaceae family, which is grown in many parts of Turkey. In this study, seven Turkish coriander varieties (Antakya, Arslan, Erbaa, Gurbuz, Kerkuk, Kudret-K, and Pelmus) were examined for phylogenetic relations using two ITS region (ITS4-ITS5) sequence information and their secondary structure analysis. The length of the nrDNA ITS sequences obtained in this study varied between 677 and 692 nucleotides for seven samples. The G + C content of the nrDNA ITS region ranged between 47.86 (ITS4) and 48.67% (ITS5). Secondary structural analysis revealed that variation between varieties is more unique. The AMOVA study confirmed the effectiveness of configuration analysis as variants accounted for the majority of the variance. In addition, variation within cultivars was significant (97%). This shows that a significant part of the variance detected in this study occurred among genotypes. Data analysis, including Shannon information index and scaled diversity overlap graph annotation, also showed that the highest level of heterozygosity occurred in the Arslan, Erbaa and Kudret-K genotypes, with cultivars showing more pronounced differences genotypically rather than geographically, consistent with the expected heterozygosity (80%). By verification of the PCoA analysis, the genotypes of Gurbuz and Arslan are very different from those of other groups. This supports the results of the cluster analysis, which showed that the Ankara-origin Gurbuz variety had a very different genotypic difference. Our analysis recommends the use of ITS4 sequence and secondary structure data at the intraspecific levels of coriander taxonomical classification. For the first time, a study has used both sequence information and secondary structural information to describe coriander at the varietal level of taxonomic classification.
Recovering Wheat (Triticum Aestivum L.) To Intake Mineral Nutrient Components Under Drought Stress With Salicylic Acid
(Parlar Scientific Publications (p S P), 2016) Arpali, Digdem; Furan, M. Alp; Ozturkci, Yusuf
This research was conducted to determine the effect of changes of certain minerals (Ca, Mg, Mn, Fe, Cu, Zn) in salicylic acid doses on different wheat varieties (Dogu-88, Altay-2000, Bezostaya-1, Alparslan and Tir mixed population) in drought stress. Drought was created in plants by irrigating 40% of field capacity. It was found that plants were differently responding to drought of mineral accumulation. 1 mM kg-1 dose of salicylic acid, compared to 0.1 mM kg-1 dose significantly increased the mineral content in flag leaf of all varieties to be statistically. The increase was found to be highest in Bezostaya 1 and Altay-2000 varieties. This research has shown that mineral contents are significantly decreased with the drought in wheat varieties, and it was concluded that salicylic acid treatment affected to all varieties positively although this effect is different according to the varieties.
Search for the New Genetic Resources Against To Drought
(Parlar Scientific Publications (p S P), 2016) Furan, M. Alp; Arpali, Digdem
The main purpose of plant breeders is to develop efficient genotypes that are high and stable under drought stress. Tir wheat (Triticum aestivum L. ssp vulgare Vill. v. leucospermum Korn) is mostly of a mixed population and it grows rapidly after germination, which minimizes yield losses caused by late planting. Because it has a long coleoptile it is capable of germinating from 12 to 15cm deep cultivation. In the study, some of bread wheat varieties and Tir genotypes were compared at three different levels of osmotic pressure and classified with SSR markers according to the coleoptile length, as well as the germination percentage of the fourth and eighth days and relative water content, root length, shoots length, and fresh weight. The aboveground and underground dry weights were also tested for differences. When considered from this perspective, in arid conditions there is an important relationship between coleoptile length and the drought resistance index and that can be used in the evaluation of drought resistance. Thus, Tir wheat should be given priority in the breeding programs against drought resistance. Tir wheat, with its long coleoptile feature, shows tolerance against drought; it should take place in breeding programs with its genetic values.
Species Identification and Germplasm Conservation of Origanum Based on Chloroplast Genes
(Springer, 2024) Furan, M. Alp
DNA barcoding is a molecular approach for species identification and germplasm conservation in the Origanum genus. This study proposes a DNA-based method to accurately identify the numerous species within this diverse genus. Four chloroplast genes (matK, rbcL, rpoB, trnL) and a non-coding sequence (psbA) were analyzed. Among the applied barcoding loci, matK exhibited the longest aligned sequence length, followed by rpoB, rbcL, psbA-trnH, and trnL. The rbcL gene showed the highest number of informative sites, while psbA-trnH displayed the highest proportion of variable sites within the Origanum genus. Phylogenetic analysis indicated that rbcL, rpoB, trnL, and psbA sequences were more effective than matK for identifying the Origanum genus, species, and subspecies. Combining multiple sequences improved accuracy. This study provides a molecular reference for herbal product identification and valuable tools for species identification and germplasm conservation within the Lamiaceae family.
Yetiştiriciliği Yapılan Bazı Türk Kişniş (Coriandrum Sativum L.) Çeşitlerinde Genetik Çeşitliliğin Issr ve Srap Marköleri Yardımıyla Değerlendirilmesi
(2017) Furan, M. Alp; Geboloğlu, Merve Dilek
Coriandrum sativum L., Umbelliferae / Apiaceae ailesine ait uçucu yağ dahil olmak üzere tıbbi bitki olarak kullanılan bir baharat bitkisidir. Farklı kısımlarındaki çeşitli kimyasal bileşenleri, antibakteriyel, antifungal ve antioksidatif olarak kullanışlıdır. Dolayısıyla gıdaların bozulmasını önleyerek raf ömrünün uzatılmasında önemli bir rol oynamaktadır. Kişnişin yaprak ve tohumları çoğunlukla geleneksel tedavide kullanılır ve uçucu yağları insanlar için toksik olmaması nedeniyle farmasötik ürünler ve parfüm bileşenlerinde yer alır. Kişniş, moleküler olarak yeterince tanımlanmamıştır. Kişniş ıslah çalışmalarıyla genetik olarak tek düze Coriandrum sativum L çeşitlerine ulaşılabilecektir. Kabul edilebilir Genotipler, germplazmdaki genetik değişkenlik miktarıyla belirlenir ve kişniş için kullanılabilir bilgiler sınırlıdır. Genetik farklılığının belirlenmesinin çalışılması ve ıslah programlarında uygun genotip kullanımının öneminin vurgulanması, kişnişteki genetik tabanı genişletmek için gereklidir. Bu çalışmada; bazı Türk kişniş çeşitleri arasındaki genetik varyasyonlar iki farklı markör tekniği kullanılarak (ISSR ve SRAP) DNA düzeyinde araştırılmış ve ticareti yapılan bu çeşitler arasındaki genetik ilişkinin belirlenmesi amaçlanmıştır. Her iki primer toplam 473 skorlanabilir bant vermiş ve her markörden elde edilen sonuçlar genotipler arasındaki filogenetik ilişkileri göstermiştir