Browsing by Author "Görgişen, G."

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Comprehensive Bioinformatical Analysis of Association of Igfl2 Expression and Methylation Profiles With Prognosis of Head and Neck Cancer
(Yuzuncu Yil Universitesi Tip Fakultesi, 2025) Görgişen, G.; Yabalak, S.
Insulin-like growth factor like (IGFL) family is a gene family that contains 11 cysteine residues, including two CC mo tifs, and has structural homology with Insulin like Growth Factor (IGF) family. Expression of IGFL genes has been detected in various tissues, including fetal skin, spinal cord, cerebellum, placenta, spleen, stomach, testis, and fetal heart. The biological functions and gene interactions of IGFL molecules have not yet been fully elucidated, but they are thought to perform similar functions to IGFs due to their structural similarities with the IGF family. Therefore, it is aimed to determined the relationship between IGFL2 expression and Head and Neck Cancer (HNSC) through bioinformatical approaches. GEPIA2, UALCAN, OncoDB, Mexpress, Cancersea and Kaplan-Meier Plotter databases were used for bioinformatical analyses. Our results showed that expression of IGFL2 increased in tumor tissues especially early stages of HNSC. In addition, we detected that promotor methylation status of IGFL2 decreased in tumor tissues. Gene correlation analyses also showed that IGFL2 expression is positively correlated with gene produ cts that are responsible for the development of HNSC such as IRS1, EGFR, CDK6. As a result, IGFL2 induces tumorigenesis in early stages of HNSC and may be used as a prognostic factor in the development of HNSC. © 2025, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Acetaminophen on Viability of Hela Cells
(Yuzuncu Yil Universitesi Tip Fakultesi, 2019) Görgişen, G.; Gülaçar, İ.M.; Öksüz, E.
Acetaminophen is a widely used analgesic to release pain and reduce fever. Overdose medication could lead to serious medical conditions such as hepatotoxicity and liver failure. In addition to its cytotoxicity effects, it has an anti-proliferative effect in human cancer cells. In this study it is aimed to determine the apoptotic effect of acetaminophen in HeLa cells. In this perspective, HeLa cells were treated with different concentrations of acetaminophen at three different exposure times. Cell viability was determined by MTT analysis. Results showed that acetaminophen inhibited viability of HeLa cells. Its cytotoxic effect depends on exposure time and concentration. Prolonged exposure of acetaminophen led to decrease in cell survival even at minimum concentrations. © 2019, Yuzuncu Yil Universitesi Tip Fakultesi. All Rights Reserved.
Overexpression of Insulin Receptor Substrate 1 (Irs1) Promotes Radioresistance in A172 Glioblastoma Cell Line
(Yuzuncu Yil Universitesi Tip Fakultesi, 2019) Görgişen, G.; Çakır, T.; Ateş, C.; Gülaçar, İ.M.; Yaren, Z.
Insulin receptor substrate 1 (IRS1) is the main adaptor molecule in insulin and insulin like growth factor signaling. Increased level of expression of IRS1 and IGF-1R proteins are associated with many human malignancies. They also induced resistance to therapeutic approaches such as chemo-and radiotherapy in cancer treatment however their molecular mechanisms are still unclear. Glioblastoma Multiforme (GBM) is the most common primary brain tumor in adults and radiotherapy has a pivotal role in its treatment. In this study, it is aimed to determine the relationship between IRS1 expression and radiosensitivity of GBM cells. Therefore, A172 cells transfected with pcDNA3.1-HA-tagged human IRS1 gene. Its overexpression was confirmed by western blot. IRS1 overexpressed A172 cells were irradiated with 5,8 and 10 Gray ionizing radiation and their effects on cell viability determined by MTT and clonogenic assay. Our results showed that overexpression of IRS1 led a decrease on sensitivity of the radiation in GBM cel ls. As a conclusion, this study should be confirmed by further molecular analysis and in vivo studies. IRS1 may be a predictive marker of radiosensitivity for GBM. © 2019, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.