Browsing by Author "Güdücüoğlu, H."

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The Determination of Meca Gene Presence in Mrsa Strains Isolated From Intensive Care Unit by Conventional, Automated and Pcr Method
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Deniz, N.Y.; Bayram, Y.; Parlak, M.; Irden, Ş.; Güdücüoğlu, H.
Meticillin-resistant S. aureus (MRSA) strains are becoming increasingly important as a cause of hospital and community-acquired infections. The aim of this study is to compare PCR, gradient tests and automated system which are the methods for determining methicillin resistance in S. aureus strains. The study included 50 MRSA strains isolated from various samples (wound, blood, sputum, respiration, abscess, osteomyelitis, etc.) from the microbiology laboratory of Van Yüzüncü Yıl University Medical Faculty between 2010-2016. A single isolate was obtained from each patient. In our study, the presence of mecA gene in MRSA strains was investigated by conventional, automated and PCR methods. The presence of methicillin resistance was found in 49 of 50 MRSA strains which were examined by Oxacillin E-test method (MIC˃2). All of the strains which were tested by cefoxitin E-test were found resistant to methicillin (MIC˃4). Vitek 2 automated system detected that all of the strains are resistant to methicillin. All samples examined by PCR method were positive, the presence of mecA gene was determined. Sensitivity rate of strains examined by oxacillin E-test method was 98%, while the susceptibility rate of strains examined by cefoxitin E-test, Vitek 2 automated system and PCR methods was 100%. In order to obtain the necessary precautions in hospitals with high MRSA ratio, mecA investigation is of great importance in achieving the correct results. Since the results of the three methods are almost close to each other, three methods can be used to determine the correct results. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Does the Coronavirus a Global Threat
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Güdücüoğlu, H.; Korkmaz, D.
Hospital Infections Related With Hospital Microbial Environment
(Yuzuncu Yil Universitesi Tip Fakultesi, 2015) Güdücüoğlu, H.
This review discusses the important role of contaminated environmental surfaces in contributing to transmission of healthcare-associated pathogens, including the ability of pathogens on dry environmental surfaces, touched by patients and healthcare workers, and so transmission to patients. Contamination of hospital equipment, medicines and water supplies with hospital pathogens is common source of outbreaks of infection. This study reviews several important pathogens including Clostridium difficile, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin- resistant enterococci (VRE), Acinetobacter baumannii, Pseudomonas aeruginosa, and norovirus that have the ability to survive in the dry-surface environment, which may then become a source for transmission. The role of contaminated environmental surfaces in transmission of healthcare-associated pathogens is also supported by the fact that cleaning and/or disinfection of the environment can reduce the incidence of healthcare-associated colonization or infection. © 2015, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Phenotypic and Genotypic Analysis for Antibiotic Resistance of Enterococcus Species With Chicken and Gull Origin
(Chartered Inst. of Building Services Engineers, 2016) Akgül, Ö.; Gülhan, T.; Güdücüoğlu, H.
In this study, faecal samples of backyard chickens in the city of Van and its districts and gulls in contact with humans in Van Lake basin have been obtained and examined for Enterecoccus species. For this purpose, 1000 faecal samples have been obtained as 500 from chickens and 500 from gulls. In the study, Entrerecoccus has been isolated and identified from a total of 311 (31.1%) faecal samples as 192 (38.4%) from chickens and 119 (23.8%) from gulls. 41 (21.3%) of chicken-origin isolates have been identified as Enterococcus faecalis, 110 (57.3%) as E. faecium, 9 (4.7%) as E. casseliflavus/gallinarum, 27 (14.1%) as E. hirae, 5 (2.6%) E. durans while 78 (65.5%) of gull-origin isolates have been identified as E. faecalis, 21 (17.6%) as E. faecium, 10 (8.4%) as E. hirae, 7 (5.9%) as E. casseliflavus/gallinarum, 2 (1.7%) as E. raffinosus and 1 (0.8%) as E. durans. Phenotypically antibiotic susceptibility testing (disc diffusion method) was performed for analysis. Genotypically 16S rRNA, 16S and 23S intergenic transcribed spacer region, esp, vanA, vanB and vanC (C-1, C-2, C-3) was analyzed genes. When antibiotic resistance of whole isolates have been taken into consideration; the highest level of resistance was determined to cefadroxil (99.5%) while the lowest resistance was determined to imipenem (0.8%). While 9 (2.9%) of the isolates have been determined as resistant to vancomycin; genotypically vancomycin resistance gene (van) has been determined 20 (6.4%) of the isolates. 6 of E. faecalis (1 chicken, 5 gull origin) and 3 of E. faecium (gull origin) isolates have been determined as carrying vanA, 6 E. casseliflavus/gallinarum as carrying vanC1 (2 chicken, 4 gull origin) and 5 E. casseliflavus/gallinarium (chicken origin) as carrying vanC2/3 gene. In gull isolates, while vanC2/3 gene was not determined in gull isolates; all chicken and gull origin isolates have been found negative for vanB gene. As a result, for the first time in our region, this research has revealed the presence of vancomycin-resistant Enterococcus species. © 2016, Chartered Inst. of Building Services Engineers. All rights reserved.