Browsing by Author "Gülbahar, MY"

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Avipoxvirus Infection in Quails
(Tubitak Scientific & Technological Research Council Turkey, 2005) Gülbahar, MY; Çabalar, M; Boynukara, B
The present study describes clinical, virological, histopathological, immunohistochemical and electron microscopic findings of pox infection encountered in a quail flock. Lesions consisted of single or multiple nodules with a crust in variable size of gray to yellow or dark brown discoloration on the comb, eyelids, and the other poorly feathered areas of the body. Histopathological changes consisted of hyperplastic epithelium overlying, with ballooning degeneration of keratinocytes, many of which had eosinophilic intracytoplasmic inclusion bodies (Bollinger bodies), and heterophilic infiltrations admixed with mononuclear cells extending into dermis. Avipoxvirus infection was confirmed by positive immunostaining in the cytoplasm of affected cells for poxvirus antigen, excluded no immunostaining of inclusion bodies, using streptavidin-biotin peroxidase complex method and by demonstration of brick-shaped virus particles with a central core using direct electron microscopy. Typical thickness of chorio-allantoic membranes (CAMs) infected with virus, compared with controls, was observed, and the harvested virus on CAMs was detected as positive against known fowlpox virus using gel diffusion test.
Comparative Study of Discolysis and Disc Fenestration in Dogs
(Scientific Technical Research Council Turkey, 1999) Besalti, Ö; Özak, A; Akin, F; Gülbahar, MY; Bumin, A
Postoperative clinical, histopathological and radiological findings were evaluated for disc fenestration, open discolysis (chymopapain) and percutan discolysis which had been performed on three groups of dogs, each group containing 6 healthy mongrels of different ages, sexes and breeds. The degrees of intervertebral space narrowing were classified as mild, medium and severe according to postoperative radiological examinations. Sections which were prepared from intervertebral areas which had been operated on, were investigated histopathologically, It was considered that discolysis might be cost effective on type-2 disc diseases, cases without advanced paralysis and cases requiring prophylactic disc fenestration, According to clinical examination results neurological disorders were not observed in the groups with disc fenestration or discolysis. Radiological and hisyopathological findings with respect to disc fenestration were more uniform than in the group which had undergone discolysis.
Dicephalus, Arnold-Chiari Malformation, Spinal Dysraphism and Other Associated Anomalies in a Newborn Holstein Calf
(Tubitak Scientific & Technological Research Council Turkey, 2005) Gülbahar, MY; Yüksel, H; Soygüder, Z; Erçin, ÖF
A case of dicephalus, Arnold-Chiari malformation, spinal dysraphism and other associated defects was described in a newborn Holstein calf. The heads were medially and caudally joined with temporal bones and one common flattened occipital bone respectively. Cranial cavities became narrow dorsoventrally and included elongation of the compressed brains, and caudal displacement and hypoplasia of cerebelli. In the spinal column. variable degrees of dysraphism such as cervico-thoracal diplomyelia with hydromyelia and syringomyelia, lumbar spina bifida with spinal cord agenesis in the region were observed. The calf had also arthrogryposis of hind limbs and cleft palate of both heads.
Immunohistochemical Localization of Cytokeratin, Vimentin and Alpa-Smooth Muscle Actin Micro and Intermediate Filaments in Canine Mammary Tumors
(Scientific Technical Research Council Turkey, 2000) Milli, UH; Haziroglu, R; Yilmaz, A; Aydin, Y; Gülbahar, MY
Forty-six formalin-fixed paraffin-embedded canine mammary tumors, 15 benign and 31 malignant, were studied immunohistochemically. Mouse monoclonal anti-alpha-cytokeratin 18, mouse monoclonal anti-alpha-cytokeratin (pan cytokeratin), mouse monoclonal anti-alpha-smooth muscle actin and mouse monoclonal anti-vimentin antibodies were used. The ductal and acinar epithelium stained positively for cytokeratin 18 and pan cytokeratin, although there was no immunoreactivity in some tumors. Metaplastic squamous cells in two solid carcinomas were positive for both cytokeratin 18 and pan cytokeratin. It was a notable finding that there were unreactive epithelial cells in some tumors for broad spectrum cytokeratin antibody (pan cytokeratin). Myofibroblasts in benign and malignant tumors were positive for both vimentin and alpha-smooth muscle actin antibodies. Stromal mesenchymal tissues, including metaplastic cartilage and bone, stained strongly positive for vimentin antibody. The majority of cells in two spindle cell carcinomas were immunoreactive with alpha-smooth muscle actin, being indicative of the myoepithelial origin of these tumors. Myoepithelial proliferation was prominent in ductal papilloma (1/1), benign mixed tumor (1/5), tubular adenocarcinomas (3/6), papillary adenocarcinomas (3/4), papillary cystic adenocarcinomas (2/3), solid carcinomas (4/8) and malignant mixed tumors (2/4). These proliferated cells stained positively for alpha-smooth muscle actin antibody except for those of a tubular adenocarcinoma, papillary adenocarcinoma and solid carcinoma. The absence of immunoreactivity with alpha-smooth muscle actin in chondromucinous stroma, cartilage and bone indicated that cartilage and bone formation in canine mammary tumors results from stromal metaplasia. The results of this study provide further evidence for the value and usefulness of immunohistochemical methods using monoclonal antibodies in formalin-fixed paraffin-embedded canine mammary tumors.
Pathological and Immunohistochemical Studies in Rabbits Experimentally Infected With Toxoplasma Gondii
(Scientific Technical Research Council Turkey, 2003) Haziroglu, R; Altintas, K; Atasever, A; Gülbahar, MY; Kul, O; Tunca, R
Experimental toxoplasmosis was induced in rabbits using different routes. A total of 42 animals were used; 36 of them were in the experimental groups and the other six comprised the control group. In addition, five guinea pigs were used for preparing hyperimmune sera against Toxoplasma gondii. The Ankara strain was inoculated intravenously (group 1, n: 12), intraperitoneally (group 11, n: 12) and intradermally (group III, n: 12) into the rabbits. Group IV animals were kept as controls (n: 6). Clinical and macroscopical findings varied according to the routes of inoculation and organs affected. Microscopically, multifocal necrosis with T. gondii tachyzoites was the main lesion in group I. In group 11, necrotic lesions started at the serosal surfaces of the abdominal organs. In both groups, lesions were distributed in and invaded the deep parts of organs in accordance with post-inoculation days. In group 111, lesions characterized by chronic inflammatory changes and tissue cysts were detected in the brain, liver and spleen in particular. Immurohistochemically, it was observed that positive reactions were not only observed in the tachyzoites and tissue cysts but also detected in antigenic clusters of destroyed organism on the serosal surfaces of the organs in the abdominal cavity.
Renal Oxalosis in a Calf
(Scientific Technical Research Council Turkey, 2002) Gülbahar, MY; Kaya, A; Gölen, I
A case of neonatal renal oxalosis in a 7-day-old calf is described. Clinical biochemistry revealed increased blood urea nitrogen. Gross findings included thin renal medulla and dilated renal calyces containing numerous, small, pale yellow and granular calculi. Histologically, most cortical and medullary tubules were dilated with birefringent crystals, whereas the glomeruli appeared unaffected. The crystals were morphologically consistent with calcium oxalate by the Pizzolato technique. Diffuse interstitial fibrosis and focal accumulations of lymphocytes were also noted.
Splenic Hemangiosarcoma With Abdominal Dissemination in a Dog
(Scientific Technical Research Council Turkey, 1998) Gülbahar, MY; Güvenç, T; Besalti, Ö
A case of spontaneosly ruptured splenic hemangiosarcoma with abdominal dissemination in a 12-year-old male German shepherd dog is described. At necropsy, a ruptured tumor mass was seen in the ventral region of the Visceral surface of the spleen, and disseminated tumor masses were seen throughout the peritoneal cavity. Histologically, in both the primary tumor mass and disseminated tumor masses, atypical endothelial cells lining vascular spaces as small clefts or as cavernous channels were observed. Some immunological properties of tumor cells were determined for endothelial cell markers (factor VII-related antigen=von Willebrand's factor [vWf] and CD31 antigen), mesenchymal tissue marker (vimentin) and pericyte-smooth muscle marker (a-smooth muscle actin [alpha-SMA]). Tumor cells were positive for CD31, vimentin and a-SMA, whereas they were negative for vWf. According to morphological and immunohistochemical results, this case was diagnosed as hemangiosarcoma arising from the spleen with abdominal dissemination.