Browsing by Author "Gülendağ, Erman"

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An Investigation of Polymorphism on the FASN, SCD1, and SLC27A3 Genes in Sheep
(2024) Ayan, Adnan; Eroglu, Mehmet; Küçük, Mürsel; Gülendağ, Erman; Koca, Davut; Turgut, Ali Osman; Önen, Muhammed Furkan
Milk traits of sheep are affected by many environmental and genetic factors. These traits are quantitative traits and are determined by many genes. This study aimed to investigate polymorphisms of milk-related Fatty-acid synthase (FASN), Stearoyl-CoA desaturase (SCD1), and solute carrier 27A3 (SLC27A3) genes in cross-bred Hamdani sheep. Blood samples were collected from 100 healthy cross-bred Hamdani ewes from the jugular vein into K3-EDTA-containing tubes. Genomic DNA was extracted using a commercial DNA isolation kit. PCR products of FASN (275 bp), SCD1 (225 bp), SLC27A3-P1 (341 bp), and SLC27A3-P2 (319 bp) were subjected to restriction fragment length polymorphism (RFLP) analysis using SSil (AciI), Cfr13I, AluI, and Hpy188III the restriction enzyme, respectively. However, all gene regions were found to be monomorphic. In the study, only TT, AA, AA, and GG genotypes were detected for FASN, SCD1, SLC27A3-P1, and SLC27A3-P2, respectively. Allele and genotype frequencies were 1.00 for all genotypes and alleles. Although this study did not reveal favorable genotypes in FASN, SCD1, and SLC27A3 genes that can be used for milk traits, more comprehensive studies with larger sample sizes should be conducted to investigate polymorphisms in cross-bred Hamdani sheep.
Milk Composition Traits of Hamdani Crossbreed Sheep Raised Under Extensive Management
(2023) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
This study aimed to reveal the milk composition traits of Hamdani crossbred sheep (Hcs) raised under extensive conditions. A total of 96 sheep milk samples were collected during the early lactation stage. Selected ewes did not have clinical mastitis or subclinical mastitis. Milk composition analyses were performed using milk autoanalyzer. Mean values for milk fat (%), solid non-fat (SNF) (%), protein (%), lactose (%), salt (%) and pH, conductivity (mS/cm), freezing point (⸰C) and density (kg/m3) in Hcs were determined as 7.49±0.15, 8.69±0.08, 4.13±0.04, 3.89±0.04, 0.62±0.01, 6.93±0.04, 4.05±0.07, -0.5±0.01, and 1.027.84±0.38 respectively. In correlation analysis, negative and significant correlations were detected between milk fat and protein (r=- 0.368, p<0.001), milk fat and SNF (r=-0375, p<0.001) milk fat and lactose (r=-0.380, p<0.001). On the other hand, positive and significant correlations were detected between SNF and milk protein (r=0.999, p<0.001), SNF and salt (r=0.963, p<0.001), and SNF and lactose (r=0.976, p<0.001). As a result, the milk fat percentage of Hcs was higher compared to other native sheep breeds of Turkey. In addition, phenotypic variation for milk fat percentage was found to be high among individuals in the same flock. These findings may contribute to new studies to improve milk composition traits in Hamdani crossbred sheep.
Norduz Koyunlarında Sütün Kimyasal Yapısı ve Fiziksel Özellikleri
(2023) Koca, Davut; Turgut, Ali Osman; Çetin, Nebi; Üner, Sefa; Gülendağ, Erman; Karagülle, Bahtiyar
Bu çalışmada, Van ili Gürpınar ilçesinde yetiştirilen Norduz koyunlarının orta laktasyon döneminde süt kompozisyon özelliklerinin ortaya konulması amaçlanmıştır. Koyunlar klinik ve subklinik mastitis yönünden değerlendirilmiş ve örnekler yalnızca sağlıklı koyunlardan toplanmıştır. Bu amaçla toplam 104 koyun sütü örneği toplandı. Norduz koyun sütünün kimyasal ve fiziksel özellikleri, süt otoanalizörü kullanılarak belirlenmiştir. Çalışmada Norduz sütünde süt yağı (%), 2.48±0.11; yağsız kuru madde (SNF) (%), 10.76±0,08; süt proteini (%), 5.09±0.04; laktoz (%), 4.79±0.04; pH, 6.93±0.02; iletkenlik (mS/cm), 4.41±0.04; donma noktası (°C),-0.602±0.80; tuz (%), 0.8035±0.66; ve yoğunluk (kg/m3), 1039.73±0.39 olarak belirlenmiştir. Süt yağı ile protein (r=-0.36, p<0.001), süt yağı ile SNF (r=-0.32, p<0.001) ve süt yağı ile laktoz (r=-0.36, p<0.001) arasında negatif ve istatistiksel olarak anlamlı korelasyonlar gözlenmiştir. SNF ile süt proteini (r=0.90, p<0.001), SNF ile tuz (r=0.87, p<0.001) ve SNF ile laktoz (r=0.90, p<0.001) arasında ise pozitif ve anlamlı ilişkiler belirlenmiştir. Özetle, Norduz koyun sütünün protein içeriği Türkiye'de yetiştirilen koyun ırklarının çoğunluğunun protein içeriğinden yüksek olduğu tespit edildi. Bu bulgular Norduz koyunlarının süt bileşiminin arttırılmasına değerli bir katkı yapma potansiyeli taşımaktadır.
Paep Gene Restriction Fragment Length Polymorphism and Its Effects on Milk Composition in Cross-Bred Hamdani Sheep
(2024) Turgut, Ali Osman; Gülendağ, Erman; Koca, Davut; Üner, Sefa
Β-lactoglobulin (βLG) stands as the primary whey protein in ruminant milk, synthesized by mammary gland cells during lactation and encoded by the βLG gene. This study aimed to assess the impact of βLG gene exon II polymorphism on milk composition traits in crossbreed Hamdani sheep. Sheep were examined for clinical diseases and mastitis. Milk and blood samples were only collected from healthy ewes. The composition and physical properties of milk were analyzed using milk autoanalyzer. The βLG gene exon II region's 452 bp PCR products were subjected to RFLP analysis using the RsaI restriction enzyme. Two genotypes, AA and AB, were identified for the βLG gene exon II region, with A and B allele frequencies of 0.7 and 0.3, respectively. Statistical analysis, conducted with Minitab® (Version: 19.2020.2.0), revealed that the AA genotype is associated with a higher milk fat percentage p<0.05). However, no significant genotype effect was observed for other milk composition traits in cross-bred Hamdani sheep. These results suggest that PAEP genotypes could serve as valuable indicators for enhancing milk composition in cross-bred Hamdani sheep through breeding programs.